Essentially clearing the structure-activity relationship between iron carbide catalysts involving multiple active centers to understand the reaction mechanism of CO hydrogenation conversion process is still a great ch...Essentially clearing the structure-activity relationship between iron carbide catalysts involving multiple active centers to understand the reaction mechanism of CO hydrogenation conversion process is still a great challenge.Here,two main micro-environment factors,namely electronic properties and geometrical effects were found to have an integrated effect on the mechanism of CO hydrogenation conversion,involving active sites on multiple crystal phases.The Bader charge of the surface Fe atoms on the active sites had a guiding effect on the CO activation pathway,while the spatial configuration of the active sites greatly affected the energy barriers of CO activation.Although the defective surfaces were more conducive to CO activation,the defective sites were not the only sites to dissociate CO,as CO always tended to dissociate in a wider area.This synergistic effect of the micro-environment also occurred during the CO conversion process.Surface C atoms on relatively flat configurations were more likely to form methane,while the electronic properties of the active sites could effectively describe the C-C coupling process,as well as distinguish the coupling mechanisms.展开更多
The irrigation method used in winter wheat fields affects micro-environment factors, such as relative humidity（RH） within canopy, soil temperature, topsoil bulk density, soil matric potential, and soil nutrients, an...The irrigation method used in winter wheat fields affects micro-environment factors, such as relative humidity（RH） within canopy, soil temperature, topsoil bulk density, soil matric potential, and soil nutrients, and these changes may affect plant root growth.An experiment was carried out to explore the effects of irrigation method on micro-environments and root distribution in a winter wheat field in the 2007–2008 and 2008–2009 growing seasons.The results showed that border irrigation（BI）, sprinkler irrigation（SI）, and surface drip irrigation（SDI） had no significant effects on soil temperature.Topsoil bulk density, RH within the canopy, soil available N distribution, and soil matric potential were significantly affected by the three treatments.The change in soil matric potential was the key reason for the altered root profile distribution patterns.Additionally, more fine roots were produced in the BI treatment when soil water content was low and topsoil bulk density was high.Root growth was most stimulated in the top soil layers and inhibited in the deep layers in the SDI treatment, followed by SI and BI, which was due to the different water application frequencies.As a result, the root profile distribution differed, depending on the irrigation method used.The root distribution pattern changes could be described by the power level variation in the exponential function.A good knowledge of root distribution patterns is important when attempting to model water and nutrient movements and when studying soil-plant interactions.展开更多
Essentially developing and regulating the unique electronic property of iron carbide catalysts involving multi-metal promoters to understand the structure–activity relationship of the CO_(2)activation process is stil...Essentially developing and regulating the unique electronic property of iron carbide catalysts involving multi-metal promoters to understand the structure–activity relationship of the CO_(2)activation process is still a great challenge.Here,metals and transition metals were introduced as strategies for electronic structure regulation to theoretically understand the underlying nature of CO_(2)adsorption and activation behaviors over Fe_(3)C catalysts.The distinctive micro-environment on the Fe_(3)C surface made it more conducive to CO_(2)adsorption and activation compared to other Fe-based catalysts,which could be further enhanced by the addition of multi-metal promotors.The electronic properties of promoters well explained their influence on CO_(2)adsorption behavior,while the binding energy of promoters had a good linear relationship with the CO_(2)activation barrier.Specifically,there existed a distance effect by the doped multi-metals.When the doped metals were adjacent to each other,it would be more beneficial for CO_(2)dissociation,and when they were far away from each other,it facilitated CO_(2)adsorption.Moreover,Mn atom is a good choice for forming dual-atom catalysts,which could significantly increase the adsorption stability of CO_(2)and facilitate the direct dissociation of CO_(2).展开更多
Elucidation the relationship between electrode potentials and heterogeneous electrocatalytic reactions has attracted widespread attention.Herein we construct the well-defined Mn single-atom(MnSA)catalyst with four N-c...Elucidation the relationship between electrode potentials and heterogeneous electrocatalytic reactions has attracted widespread attention.Herein we construct the well-defined Mn single-atom(MnSA)catalyst with four N-coordination through a simple thermal pyrolysis preparation method to investigate the electrode potential micro-environments effect on carbon dioxide reduction reactions(CO_(2)RR)and oxygen reduction reactions(ORR).MnSA catalysts generate higher CO production Faradaic efficiency of exceeding 90%at-0.9 V for CO_(2)RR and higher H_(2)O_(2)yield from 0.1 to 0.6 V with excellent ORR activity.Density functional theory(DFT)calculations based on constant potential models were performed to study the mechanism of MnSA on CO_(2)RR.The thermodynamic energy barrier of CO_(2)RR is lowest at-0.9 V vs.reversible hydrogen electrode(RHE).Similar DFT calculations on the H_(2)O_(2)yield of ORR showed that the H_(2)O_(2)yield at 0.2 V was higher.This study provides a reasonable explanation for the role of electrode potential micro-environments.展开更多
Background:Recurrent implantation failure(RIF)is a difficult problem with a multifaceted cause.Recent studies have demonstrated that stimulator of interferon genes-induced immune-related genes(STIRGs)are associated wi...Background:Recurrent implantation failure(RIF)is a difficult problem with a multifaceted cause.Recent studies have demonstrated that stimulator of interferon genes-induced immune-related genes(STIRGs)are associated with immune disorders that may affect the endometrial immune micro-environment.However,the effect of STRIGs on RIF remains unknown.Methods:Training(GSE111974)and validation(GSE106602)cohorts were acquired from the Gene Expression Omnibus database.STIRGs were extracted from the Molecular Signatures Database and relevant studies.Consensus clustering analysis was used to identify RIF molecular subtypes.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment and immune infiltration analyses were performed between RIF subtypes.Drug-related potential therapeutic target genes were discovered.Results:Two distinct molecular subtypes were discovered in both the training and validation groups according to STIRGs.In subtype C2,there was a notable decrease in the presence of different types of immune cells,such as natural killer cells and macrophages.Furthermore,the examination of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes indicated a decrease in numerous immune-related biological processes within subtype C2.Finally,nine hub genes(CXCR4,POU5F1,PPARG,TLR2,EGFR,CSF1,BCL2A1,BTK,and SRGN)were identified as potential therapeutic targets for RIF.Conclusion:Based on STIRGs,we identified a new molecular subtype with significantly reduced immune infiltration in RIF.Nine genes might be potential therapeutic targets for RIF.展开更多
Surface functionalization of Cu-based catalysts has demonstrated promising potential for enhancing the electrochemical CO_(2)reduction reaction(CO_(2)RR)toward multi-carbon(C2+)products,primarily by suppressing the pa...Surface functionalization of Cu-based catalysts has demonstrated promising potential for enhancing the electrochemical CO_(2)reduction reaction(CO_(2)RR)toward multi-carbon(C2+)products,primarily by suppressing the parasitic hydrogen evolution reaction and facilitating a localized CO_(2)/CO concentration at the electrode.Building upon this approach,we developed surface-functionalized catalysts with exceptional activity and selectivity for electrocatalytic CO_(2)RR to C_(2+)in a neutral electrolyte.Employing CuO nanoparticles coated with hexaethynylbenzene organic molecules(HEB-CuO NPs),a remarkable C_(2+)Faradaic efficiency of nearly 90%was achieved at an unprecedented current density of 300 mA cm^(-2),and a high FE(>80%)was maintained at a wide range of current densities(100-600 mA cm^(-2))in neutral environments using a flow cell.Furthermore,in a membrane electrode assembly(MEA)electrolyzer,86.14%FEC2+was achieved at a partial current density of 387.6 mA cm^(-2)while maintaining continuous operation for over 50 h at a current density of 200 mA cm^(-2).In-situ spectroscopy studies and molecular dynamics simulations reveal that reducing the coverage of coordinated K⋅H2O water increased the probability of intermediate reactants(CO)interacting with the surface,thereby promoting efficient C-C coupling and enhancing the yield of C_(2+)products.This advancement offers significant potential for optimizing local micro-environments for sustainable and highly efficient C_(2+)production.展开更多
Dual atomic catalysts(DAC),particularly copper(Cu_(2))-based nitrogen(N)doped graphene,show great potential to effectively convert CO_(2)and nitrate(NO_(3)-)into important industrial chemicals such as ethylene,glycol,...Dual atomic catalysts(DAC),particularly copper(Cu_(2))-based nitrogen(N)doped graphene,show great potential to effectively convert CO_(2)and nitrate(NO_(3)-)into important industrial chemicals such as ethylene,glycol,acetamide,and urea through an efficient catalytical process that involves C–C and C–N coupling.However,the origin of the coupling activity remained unclear,which substantially hinders the rational design of Cu-based catalysts for the N-integrated CO_(2)reduction reaction(CO_(2)RR).To address this challenge,this work performed advanced density functional theory calculations incorporating explicit solvation based on a Cu_(2)-based N-doped carbon(Cu_(2)N_(6)C_(10))catalyst for CO_(2)RR.These calculations are aimed to gain insight into the reaction mechanisms for the synthesis of ethylene,acetamide,and urea via coupling in the interfacial reaction micro-environment.Due to the sluggishness of CO_(2),the formation of a solvation electric layer by anions(F^(-),Cl^(-),Br^(-),and I^(-))and cations(Na+,Mg^(2+),K+,and Ca^(2+))leads to electron transfer towards the Cu surface.This process significantly accelerates the reduction of CO_(2).These results reveal that*CO intermediates play a pivotal role in N-integrated CO_(2)RR.Remarkably,the Cu_(2)-based N-doped carbon catalyst examined in this study has demonstrated the most potential for C–N coupling to date.Our findings reveal that through the process of a condensation reaction between*CO and NH_(2)OH for urea synthesis,*NO_(3)-is reduced to*NH_(3),and*CO_(2)to*CCO at dual Cu atom sites.This dual-site reduction facilitates the synthesis of acetamide through a nucleophilic reaction between NH_(3)and the ketene intermediate.Furthermore,we found that the I-and Mg^(2+)ions,influenced by pH,were highly effective for acetamide and ammonia synthesis,except when F-and Ca^(2+)were present.Furthermore,the mechanisms of C–N bond formation were investigated via ab-initio molecular dynamics simulations,and we found that adjusting the micro-environment can change the dominant side reaction,shifting from hydrogen production in acidic conditions to water reduction in alkaline ones.This study introduces a novel approach using ion-H_(2)O cages to significantly enhance the efficiency of C–N coupling reactions.展开更多
Invasive inflammation and excessive scar formation are the main reasons for the difficulty in repairing nervous tissue after spinal cord injury.Microglia and astrocytes play key roles in the spinal cord injury micro-e...Invasive inflammation and excessive scar formation are the main reasons for the difficulty in repairing nervous tissue after spinal cord injury.Microglia and astrocytes play key roles in the spinal cord injury micro-environment and share a close interaction.However,the mechanisms involved remain unclear.In this study,we found that after spinal cord injury,resting microglia(M0)were polarized into pro-inflammatory phenotypes(MG1 and MG3),while resting astrocytes were polarized into reactive and scar-forming phenotypes.The expression of growth arrest-specific 6(Gas6)and its receptor Axl were significantly down-regulated in microglia and astrocytes after spinal cord injury.In vitro experiments showed that Gas6 had negative effects on the polarization of reactive astrocytes and pro-inflammatory microglia,and even inhibited the cross-regulation between them.We further demonstrated that Gas6 can inhibit the polarization of reactive astrocytes by suppressing the activation of the Yes-associated protein signaling pathway.This,in turn,inhibited the polarization of pro-inflammatory microglia by suppressing the activation of the nuclear factor-κB/p65 and Janus kinase/signal transducer and activator of transcription signaling pathways.In vivo experiments showed that Gas6 inhibited the polarization of pro-inflammatory microglia and reactive astrocytes in the injured spinal cord,thereby promoting tissue repair and motor function recovery.Overall,Gas6 may play a role in the treatment of spinal cord injury.It can inhibit the inflammatory pathway of microglia and polarization of astrocytes,attenuate the interaction between microglia and astrocytes in the inflammatory microenvironment,and thereby alleviate local inflammation and reduce scar formation in the spinal cord.展开更多
This editorial aimed to consolidate current evidence on the role of major endogenous modulators—nitric oxide(NO),prostaglandins(PGs),thromboxanes(TXs),and endothelins(ETs)in the lung carcinogenesis,their receptor-spe...This editorial aimed to consolidate current evidence on the role of major endogenous modulators—nitric oxide(NO),prostaglandins(PGs),thromboxanes(TXs),and endothelins(ETs)in the lung carcinogenesis,their receptor-specific actions,compensatory feedback mechanisms,and their role in tumor immune evasion and angiogenesis.We searched PubMed and Google Scholar with free-text and MeSH combinations of terms including"lung cancer","nitric oxide","inducible NOS","COX-2","prostaglandin E2","thromboxane A2","endothelin","angiogenesis",and"immunosuppression".We examined English-language publications for mechanistic data,preclinical models,and clinical correlates,and synthesized findings from both animal and human tissue studies.We highlight here the dual,concentration-dependent actions of NO,PG-E2's immunosup-pressive and pro-angiogenic actions via E-Prostanoid(EP2/EP4)receptors,thromboxane A2's pro-metastatic functions by thromboxane receptor signaling and interaction with platelet-tumor interaction,and the underappreciated roles of ETs.We also point to gaps in the existing literature on the differential roles of other prostanoid subtypes(e.g.,PGI2,PGD2),hypoxia-inducible factor-1α's role in regulation of inflammatory cascades,and clinical significance of compensatory upregulation of TX synthase following cycloxygenase-2 inhibition.These obse-rvations underscore the potential need for receptor-targeted therapies,biomarker-guided patient stratification,and improved translational models to inform the development of personalized anti-inflammatory interventions in lung cancer.展开更多
The integrated lipopeptide(RVA)/gene complexes are fabricated with bi-directional regulation on tumor cells and micro-environment.After self-assembling and target coating modification,the poly(γ-glutamic acid)(γ-PGA...The integrated lipopeptide(RVA)/gene complexes are fabricated with bi-directional regulation on tumor cells and micro-environment.After self-assembling and target coating modification,the poly(γ-glutamic acid)(γ-PGA)/RVA nano-vectors can sequentially respond to pH&redox stimuli,and guarantee efficient therapeutic gene delivery and control release of all-trans retinoic acid.The design provides a facile but promising strategy to treat refractory cancers.展开更多
A three-dimensional （3D） mathematical model of tumour growth at the avascular phase and vessel remodelling in host tissues is proposed with emphasis on the study of the interactions of tumour growth and hypoxic micr...A three-dimensional （3D） mathematical model of tumour growth at the avascular phase and vessel remodelling in host tissues is proposed with emphasis on the study of the interactions of tumour growth and hypoxic micro-environment in host tissues. The hybrid based model includes the continuum part, such as the distributions of oxygen and vascular endothelial growth factors （VEGFs）, and the discrete part of tumour cells （TCs） and blood vessel networks. The simulation shows the dynamic process of avascular tumour growth from a fewinitial cells to an equilibrium state with varied vessel networks. After a phase of rapidly increasing numbers of the TCs, more and more host vessels collapse due to the stress caused by the growing tumour. In addition, the consumption of oxygen expands with the enlarged tumour region. The study also discusses the effects of certain factors on tumour growth, including the density and configuration of pre- existing vessel networks and the blood oxygen content. The model enables us to examine the relationship between early tumour growth and hypoxic micro-environment in host tissues, which can be useful for further applications, such as tumour metastasis and the initialization of tumour angiogenesis.展开更多
Three kinds of packaging bags composited with barrier film and moisture absorbents were designed in order to find out how to create an internal micro-environment with low O2content and relative humidity( RH). Peanuts ...Three kinds of packaging bags composited with barrier film and moisture absorbents were designed in order to find out how to create an internal micro-environment with low O2content and relative humidity( RH). Peanuts with different moisture contents were filled into packaging bags and stored in an environmental chamber for a certain period of time. An RH recorder was sealed in each bag and the RH inside the bag was recorded. The moisture contents of peanuts before and after storage were measured as well.It is found that peanuts with moisture content of 13% can reduce O2content inside the bag by the aerobic respiration,achieving the self modified atmosphere packaging( MAP) effect. A microenvironment with lower RH can be created by using super-absorbent fiber( SAF) or mineral bag as the moisture absorbent. The moisture contents of peanuts stored in the bags with SAF and mineral bag as moisture absorbent decreased from 13% to 10. 5%and 11. 3%,respectively. SAF is a promising material of packaging bags composited with barrier film,in which a low RH and high CO2content can be created.展开更多
A small English class can be called as a micro-environment for English study. In this small micro-environment, the relationship between teachers and students is subtle. How to achieve the good interaction and create a...A small English class can be called as a micro-environment for English study. In this small micro-environment, the relationship between teachers and students is subtle. How to achieve the good interaction and create a good micro-environment in English class between teachers and students is very important for developing students＇ learning ability. This thesis is based on Rogers＇ humanistic teaching theory, which uses questionnaires, inteview and comparative analysis to analyze the current situation of micro-environment in college English learning. It discusses the construction of micro-environment in college English learning based on Rogers＇ humanistic teaching theory and the ways to construct it from software conditions （roles of teachers and students, relationship between teachers and students, the classroom atmosphere） and hardware conditions（teaching resources, a classroom）.展开更多
Cancer is the most common cause of human mortality and has created an unbridgeable health gap due to its unrestrained aberrant proliferation,rapid growth,metastasis,and high heterogeneity.Conventional two-dimensional ...Cancer is the most common cause of human mortality and has created an unbridgeable health gap due to its unrestrained aberrant proliferation,rapid growth,metastasis,and high heterogeneity.Conventional two-dimensional cell culture and animal models for tumor diagnostic and therapeutic studies have extremely low clinical translation rates due to their intrinsic limitations.Appropriate tumor models are therefore required for cancer research.Engineered human three-dimensional(3D)cancer models stand out for their ability to better replicate the spatial organization,cellular resources,and microenvironmental features(e.g.,hypoxia,necrosis,and delayed proliferation)of actual human tumors.Further,the fabrication of these models can be achieved by an emerging technology known as 3D bioprinting,which allows for the fabrication of living structures by precisely regulating the spatial distribution of cells,biomolecules,and matrix components.The aim of this paper is to review the current technologies and bioinks associated with 3D bioprinted cancer models for glioma,breast,liver,intestinal,cervical,ovarian,and neuroblastoma,as well as,advances in the applications of 3D bioprinted-based tumor models in the fields of tumor microenvironment,tumor vascularization,tumor stem cells,tumor resistance and therapeutic drug screening,tumorimmunotherapy,and precision medicine.展开更多
BACKGROUND Colorectal cancer(CRC)prognosis prediction is currently a major challenge.Epigenetic regulation has been widely reported for its role in cancer development.AIM To construct a robust prognostic signature,we ...BACKGROUND Colorectal cancer(CRC)prognosis prediction is currently a major challenge.Epigenetic regulation has been widely reported for its role in cancer development.AIM To construct a robust prognostic signature,we used developed and validated across datasets.METHODS After constructing the signature,the prognostic value of the signature was evaluated in the TCGA cohort and six independent datasets(GSE17526,GSE17537,GSE33113,GSE37892,GSE39048 and GSE39582).The clinical,genomic and transcriptomic features related to the signature were identified.The correlations of the signature score with immune cell infiltration and cell-cell interactions were analyzed.The correlations between the signature score and the sensitivity to different drugs were also predicted.RESULTS In the TCGA cohort,patients in the low-risk group according to the signature score had longer survival than those in the high-risk group,and this finding was validated in the validation datasets.The signature was a prognostic factor independent of age and sex and was correlated with stage and PD-1/PD-L1 expression.Area under the receiving operating characteristic curve was 0.72.Genomic association analyses revealed that samples from highrisk patients exhibited chromosomal instability.Transcriptomic analyses revealed that the signature score was significantly associated with multiple cellular pathways.Bulk RNA-seq and single-cell sequencing data revealed that the signature reflected differences in infiltrating immune cell-tumor cell interactions,especially for macrophages.The signature also predicted the putative drug sensitivity of CRC samples.CONCLUSION The signature is a valuable biomarker for predicting CRC prognosis and reflects multiple features of CRC,especially macrophage infiltration in the microenvironment.展开更多
Optical sensors are widely used in the field of analytical sensing and optical imaging because of their high sensitivity, fast response time, and technical simplicity. The advantages of sensors lay in their non- invas...Optical sensors are widely used in the field of analytical sensing and optical imaging because of their high sensitivity, fast response time, and technical simplicity. The advantages of sensors lay in their non- invasive approach within biological tissues and inherent reliability in fluorescence imaging tests. In recent years the development of optical sensors for the sensing of ions, neutral molecules and especially tumor micro-environment-related parameters has been an active research area. This review focuses on recent contributions concerning fluorescent or colorimetric sensors and is organized according to their target classifications.展开更多
Colorectal cancer(CRC) constitutes the third most frequently reported malignancy in the male population and the second most common in women in the last two decades. Colon carcinogenesis is a complex, multifactorial ev...Colorectal cancer(CRC) constitutes the third most frequently reported malignancy in the male population and the second most common in women in the last two decades. Colon carcinogenesis is a complex, multifactorial event, resulting from genetic and epigenetic aberrations, the impact of environmental factors, as well as the disturbance of the gut microbial ecosystem. The relationship between the intestinal microbiome and carcinogenesis was relatively undervalued in the last decade. However, its remarkable effect on metabolic and immune functions on the host has been in the spotlight as of recent years. There is a strong relationship between gut microbiome dysbiosis, bowel pathogenicity and responsiveness to anti-cancer treatment;including immunotherapy. Modifications of bacteriome consistency are closely associated with the immunologic response to immunotherapeutic agents. This condition that implies the necessity of gut microbiome manipulation. Thus, creatingan optimal response for CRC patients to immunotherapeutic agents. In this paper, we will review the current literature observing how gut microbiota influence the response of immunotherapy on CRC patients.展开更多
Based on previous research, the sampling and analysis methods for phthalate esters （PAEs） were improved by increasing the sampling flow of indoor air from 1 to 4 L/min, shortening the sampling duration from 8 to 2 h...Based on previous research, the sampling and analysis methods for phthalate esters （PAEs） were improved by increasing the sampling flow of indoor air from 1 to 4 L/min, shortening the sampling duration from 8 to 2 hr. Meanwhile, through the optimization of chromatographic conditions, the concentrations of 9 additional PAE pollutants in indoor air were measured. The optimized chromatographic conditions required a similar amount of time for analysis as before, but gave high responsivity, the capability of simultaneously distinguishing 15 kinds of PAEs, and a high level of discrimination between individual sample peaks, as well as stable peak generation. The recovery rate of all gas-phase and particle-phase samples of the 15 kinds of PAEs ranged from 91.26% to 109.42%, meeting the quantitative analysis requirements for indoor and outdoor air sampling and analysis. For the first time, investigation of the concentration levels as well as characteristics of 15 kinds of PAEs in the indoor air from four different traffic micro-environments （private vehicles, busses, taxis and subways） was carried out, along with validation of the optimized sampling and analytical method. The results show that all the 9 additional PAEs could be detected at relatively high pollution levels in the indoor air from the four traffic micro-environments. As none of the pollution levels of the 15 kinds of PAEs in the indoor air from the 4 traffic micro-environments should be neglected, it is of great significance to increase the types of PAEs able to be detected in indoor air.展开更多
Human T cells are a highly heterogeneous population and can recognize a wide variety of antigens by their T cell receptors(TCRs). Tumor cells display a large repertoire of antigens that serve as potential targets for ...Human T cells are a highly heterogeneous population and can recognize a wide variety of antigens by their T cell receptors(TCRs). Tumor cells display a large repertoire of antigens that serve as potential targets for recognition,thus making T cells in the tumor micro-environment more complicated. Making a connection between TCRs and the transcriptional information of individual T cells will be interesting for investigating clonal expansion within T cell populations under pathologic conditions. Advances in single cell RNA-sequencing(scRNA-seq) have allowed for comprehensive analysis of T cells. In this review, we briefly describe the research progress on tumor microenvironment T cells using single cell RNA sequencing, and then discuss how scRNA-seq can be used to resolve immune system heterogeneity in health and disease. Finally, we point out future directions in this field and potential for immunotherapy.展开更多
Hyperplastic scar is a common fibrotic disease that may ultimately lead to severe dysfunction anddeformity,causing physical and psychological distress.Therefore,we aim to evaluate the effect of the mechanicalmicroenvi...Hyperplastic scar is a common fibrotic disease that may ultimately lead to severe dysfunction anddeformity,causing physical and psychological distress.Therefore,we aim to evaluate the effect of the mechanicalmicroenvironment of scar substrates on the morphology of human fibroblasts(HFbs).The micro-modular fabrication technique was used to design a new cross-groove topology and to construct four elastic substrates withthe stiffness of 19.3 kPa and 90.1 kPa coupled with parallel groove and cross groove,respectively,to simulate themechanical microenvironment of skin wounds and scar tissues.The morphological changes in HFbs in differentsubstrates were observed,and the changes in the cell-long axis length,area,and the angle between cell-long axisand grooves were recorded.Immunofluorescence staining was performed to observe the distribution of microfilaments.The results indicated that substrate stiffness and topography affected the morphology of HFbs.The cellswere elongated in parallel grooves as well as in the area where cross grooves restricted groove length,the celllength was restricted,and the angle between the long axis and the groove was increased.The topography exertedno significant effect on the cell area,but the cell area increased with increasing the stiffness.The parallel groovepromoted the expression of the F-actin to a certain extent,and the fluorescence intensity of F-actin decreased withincreasing the stiffness.Studying the effect of the mechanical microenvironment of substrates on HFb morphologyis of great importance for understanding the mechanisms of scar formation and prevention.展开更多
基金supported by the Research Fund for National Key Research and Development Program of China(2022YFA1503804,2021YFA1501403)the Natural Science Foundation of China(22208094,21922803,92034301,22008066 and 21776077)+2 种基金the China Postdoctoral Science Foundation(BX20190116)the Innovation Program of Shanghai Municipal Education Commission(17ZR1407300)the Program of Shanghai Academic/Technology Research Leader(21XD1421000).
文摘Essentially clearing the structure-activity relationship between iron carbide catalysts involving multiple active centers to understand the reaction mechanism of CO hydrogenation conversion process is still a great challenge.Here,two main micro-environment factors,namely electronic properties and geometrical effects were found to have an integrated effect on the mechanism of CO hydrogenation conversion,involving active sites on multiple crystal phases.The Bader charge of the surface Fe atoms on the active sites had a guiding effect on the CO activation pathway,while the spatial configuration of the active sites greatly affected the energy barriers of CO activation.Although the defective surfaces were more conducive to CO activation,the defective sites were not the only sites to dissociate CO,as CO always tended to dissociate in a wider area.This synergistic effect of the micro-environment also occurred during the CO conversion process.Surface C atoms on relatively flat configurations were more likely to form methane,while the electronic properties of the active sites could effectively describe the C-C coupling process,as well as distinguish the coupling mechanisms.
基金the National Natural Science Foundation of China(51109214,31101074 and 51309211)the National Key Technology Research and Development Program of China(2011BAD32B)the Basic Scientific Research Foundation of National Non-Profit Scientific Institute of China(BSRF201303)
文摘The irrigation method used in winter wheat fields affects micro-environment factors, such as relative humidity（RH） within canopy, soil temperature, topsoil bulk density, soil matric potential, and soil nutrients, and these changes may affect plant root growth.An experiment was carried out to explore the effects of irrigation method on micro-environments and root distribution in a winter wheat field in the 2007–2008 and 2008–2009 growing seasons.The results showed that border irrigation（BI）, sprinkler irrigation（SI）, and surface drip irrigation（SDI） had no significant effects on soil temperature.Topsoil bulk density, RH within the canopy, soil available N distribution, and soil matric potential were significantly affected by the three treatments.The change in soil matric potential was the key reason for the altered root profile distribution patterns.Additionally, more fine roots were produced in the BI treatment when soil water content was low and topsoil bulk density was high.Root growth was most stimulated in the top soil layers and inhibited in the deep layers in the SDI treatment, followed by SI and BI, which was due to the different water application frequencies.As a result, the root profile distribution differed, depending on the irrigation method used.The root distribution pattern changes could be described by the power level variation in the exponential function.A good knowledge of root distribution patterns is important when attempting to model water and nutrient movements and when studying soil-plant interactions.
基金supported by the Research Fund for National Key Research and Development Program of China(Nos.2021YFA1501403 and 2021YFB3500903)the National Natural Science Foundation of China(Nos.22208094,21922803,92034301,22008066,and 21776077)+2 种基金the China Postdoctoral Science Foundation(No.BX20190116)the Innovation Program of Shanghai Municipal Education Commission(No.17ZR1407300)the Program of Shanghai Academic/Technology Research Leader(No.21XD1421000).
文摘Essentially developing and regulating the unique electronic property of iron carbide catalysts involving multi-metal promoters to understand the structure–activity relationship of the CO_(2)activation process is still a great challenge.Here,metals and transition metals were introduced as strategies for electronic structure regulation to theoretically understand the underlying nature of CO_(2)adsorption and activation behaviors over Fe_(3)C catalysts.The distinctive micro-environment on the Fe_(3)C surface made it more conducive to CO_(2)adsorption and activation compared to other Fe-based catalysts,which could be further enhanced by the addition of multi-metal promotors.The electronic properties of promoters well explained their influence on CO_(2)adsorption behavior,while the binding energy of promoters had a good linear relationship with the CO_(2)activation barrier.Specifically,there existed a distance effect by the doped multi-metals.When the doped metals were adjacent to each other,it would be more beneficial for CO_(2)dissociation,and when they were far away from each other,it facilitated CO_(2)adsorption.Moreover,Mn atom is a good choice for forming dual-atom catalysts,which could significantly increase the adsorption stability of CO_(2)and facilitate the direct dissociation of CO_(2).
基金supported by the National Natural Science Foundation of China(Nos.52073214 and 22075211)Guangxi Natural Science Fund for Distinguished Young Scholars(No.2024GXNSFFA010008).
文摘Elucidation the relationship between electrode potentials and heterogeneous electrocatalytic reactions has attracted widespread attention.Herein we construct the well-defined Mn single-atom(MnSA)catalyst with four N-coordination through a simple thermal pyrolysis preparation method to investigate the electrode potential micro-environments effect on carbon dioxide reduction reactions(CO_(2)RR)and oxygen reduction reactions(ORR).MnSA catalysts generate higher CO production Faradaic efficiency of exceeding 90%at-0.9 V for CO_(2)RR and higher H_(2)O_(2)yield from 0.1 to 0.6 V with excellent ORR activity.Density functional theory(DFT)calculations based on constant potential models were performed to study the mechanism of MnSA on CO_(2)RR.The thermodynamic energy barrier of CO_(2)RR is lowest at-0.9 V vs.reversible hydrogen electrode(RHE).Similar DFT calculations on the H_(2)O_(2)yield of ORR showed that the H_(2)O_(2)yield at 0.2 V was higher.This study provides a reasonable explanation for the role of electrode potential micro-environments.
文摘Background:Recurrent implantation failure(RIF)is a difficult problem with a multifaceted cause.Recent studies have demonstrated that stimulator of interferon genes-induced immune-related genes(STIRGs)are associated with immune disorders that may affect the endometrial immune micro-environment.However,the effect of STRIGs on RIF remains unknown.Methods:Training(GSE111974)and validation(GSE106602)cohorts were acquired from the Gene Expression Omnibus database.STIRGs were extracted from the Molecular Signatures Database and relevant studies.Consensus clustering analysis was used to identify RIF molecular subtypes.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment and immune infiltration analyses were performed between RIF subtypes.Drug-related potential therapeutic target genes were discovered.Results:Two distinct molecular subtypes were discovered in both the training and validation groups according to STIRGs.In subtype C2,there was a notable decrease in the presence of different types of immune cells,such as natural killer cells and macrophages.Furthermore,the examination of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes indicated a decrease in numerous immune-related biological processes within subtype C2.Finally,nine hub genes(CXCR4,POU5F1,PPARG,TLR2,EGFR,CSF1,BCL2A1,BTK,and SRGN)were identified as potential therapeutic targets for RIF.Conclusion:Based on STIRGs,we identified a new molecular subtype with significantly reduced immune infiltration in RIF.Nine genes might be potential therapeutic targets for RIF.
基金supported by the National Natural Science Foundation of China(22101182)the Shenzhen Science and Technology Program(Nos.JCYJ20210324095202006,JCYJ20220531095813031,and JCYJ20230807140700001)Guangdong Basic and Applied Basic Research Foundation(2022A1515010318).
文摘Surface functionalization of Cu-based catalysts has demonstrated promising potential for enhancing the electrochemical CO_(2)reduction reaction(CO_(2)RR)toward multi-carbon(C2+)products,primarily by suppressing the parasitic hydrogen evolution reaction and facilitating a localized CO_(2)/CO concentration at the electrode.Building upon this approach,we developed surface-functionalized catalysts with exceptional activity and selectivity for electrocatalytic CO_(2)RR to C_(2+)in a neutral electrolyte.Employing CuO nanoparticles coated with hexaethynylbenzene organic molecules(HEB-CuO NPs),a remarkable C_(2+)Faradaic efficiency of nearly 90%was achieved at an unprecedented current density of 300 mA cm^(-2),and a high FE(>80%)was maintained at a wide range of current densities(100-600 mA cm^(-2))in neutral environments using a flow cell.Furthermore,in a membrane electrode assembly(MEA)electrolyzer,86.14%FEC2+was achieved at a partial current density of 387.6 mA cm^(-2)while maintaining continuous operation for over 50 h at a current density of 200 mA cm^(-2).In-situ spectroscopy studies and molecular dynamics simulations reveal that reducing the coverage of coordinated K⋅H2O water increased the probability of intermediate reactants(CO)interacting with the surface,thereby promoting efficient C-C coupling and enhancing the yield of C_(2+)products.This advancement offers significant potential for optimizing local micro-environments for sustainable and highly efficient C_(2+)production.
基金National Natural Science Foundation of China(U22B20149,22308376)Outstanding Young Scholars Foundation of China University of Petroleum(Beijing)(2462023BJRC015)Foundation of United Institute for Carbon Neutrality(CNIF20230209)。
文摘Dual atomic catalysts(DAC),particularly copper(Cu_(2))-based nitrogen(N)doped graphene,show great potential to effectively convert CO_(2)and nitrate(NO_(3)-)into important industrial chemicals such as ethylene,glycol,acetamide,and urea through an efficient catalytical process that involves C–C and C–N coupling.However,the origin of the coupling activity remained unclear,which substantially hinders the rational design of Cu-based catalysts for the N-integrated CO_(2)reduction reaction(CO_(2)RR).To address this challenge,this work performed advanced density functional theory calculations incorporating explicit solvation based on a Cu_(2)-based N-doped carbon(Cu_(2)N_(6)C_(10))catalyst for CO_(2)RR.These calculations are aimed to gain insight into the reaction mechanisms for the synthesis of ethylene,acetamide,and urea via coupling in the interfacial reaction micro-environment.Due to the sluggishness of CO_(2),the formation of a solvation electric layer by anions(F^(-),Cl^(-),Br^(-),and I^(-))and cations(Na+,Mg^(2+),K+,and Ca^(2+))leads to electron transfer towards the Cu surface.This process significantly accelerates the reduction of CO_(2).These results reveal that*CO intermediates play a pivotal role in N-integrated CO_(2)RR.Remarkably,the Cu_(2)-based N-doped carbon catalyst examined in this study has demonstrated the most potential for C–N coupling to date.Our findings reveal that through the process of a condensation reaction between*CO and NH_(2)OH for urea synthesis,*NO_(3)-is reduced to*NH_(3),and*CO_(2)to*CCO at dual Cu atom sites.This dual-site reduction facilitates the synthesis of acetamide through a nucleophilic reaction between NH_(3)and the ketene intermediate.Furthermore,we found that the I-and Mg^(2+)ions,influenced by pH,were highly effective for acetamide and ammonia synthesis,except when F-and Ca^(2+)were present.Furthermore,the mechanisms of C–N bond formation were investigated via ab-initio molecular dynamics simulations,and we found that adjusting the micro-environment can change the dominant side reaction,shifting from hydrogen production in acidic conditions to water reduction in alkaline ones.This study introduces a novel approach using ion-H_(2)O cages to significantly enhance the efficiency of C–N coupling reactions.
基金supported by the National Natural Science Foundation of China, Nos.81971151 (to YW), 82102528 (to XL), 82102583 (to LW)the Natural Science Foundation of Guangdong Province, China, Nos.2020A1515010265 (to YW), 2020A1515110679 (to XL), and 2021A1515010358 (to XL)
文摘Invasive inflammation and excessive scar formation are the main reasons for the difficulty in repairing nervous tissue after spinal cord injury.Microglia and astrocytes play key roles in the spinal cord injury micro-environment and share a close interaction.However,the mechanisms involved remain unclear.In this study,we found that after spinal cord injury,resting microglia(M0)were polarized into pro-inflammatory phenotypes(MG1 and MG3),while resting astrocytes were polarized into reactive and scar-forming phenotypes.The expression of growth arrest-specific 6(Gas6)and its receptor Axl were significantly down-regulated in microglia and astrocytes after spinal cord injury.In vitro experiments showed that Gas6 had negative effects on the polarization of reactive astrocytes and pro-inflammatory microglia,and even inhibited the cross-regulation between them.We further demonstrated that Gas6 can inhibit the polarization of reactive astrocytes by suppressing the activation of the Yes-associated protein signaling pathway.This,in turn,inhibited the polarization of pro-inflammatory microglia by suppressing the activation of the nuclear factor-κB/p65 and Janus kinase/signal transducer and activator of transcription signaling pathways.In vivo experiments showed that Gas6 inhibited the polarization of pro-inflammatory microglia and reactive astrocytes in the injured spinal cord,thereby promoting tissue repair and motor function recovery.Overall,Gas6 may play a role in the treatment of spinal cord injury.It can inhibit the inflammatory pathway of microglia and polarization of astrocytes,attenuate the interaction between microglia and astrocytes in the inflammatory microenvironment,and thereby alleviate local inflammation and reduce scar formation in the spinal cord.
文摘This editorial aimed to consolidate current evidence on the role of major endogenous modulators—nitric oxide(NO),prostaglandins(PGs),thromboxanes(TXs),and endothelins(ETs)in the lung carcinogenesis,their receptor-specific actions,compensatory feedback mechanisms,and their role in tumor immune evasion and angiogenesis.We searched PubMed and Google Scholar with free-text and MeSH combinations of terms including"lung cancer","nitric oxide","inducible NOS","COX-2","prostaglandin E2","thromboxane A2","endothelin","angiogenesis",and"immunosuppression".We examined English-language publications for mechanistic data,preclinical models,and clinical correlates,and synthesized findings from both animal and human tissue studies.We highlight here the dual,concentration-dependent actions of NO,PG-E2's immunosup-pressive and pro-angiogenic actions via E-Prostanoid(EP2/EP4)receptors,thromboxane A2's pro-metastatic functions by thromboxane receptor signaling and interaction with platelet-tumor interaction,and the underappreciated roles of ETs.We also point to gaps in the existing literature on the differential roles of other prostanoid subtypes(e.g.,PGI2,PGD2),hypoxia-inducible factor-1α's role in regulation of inflammatory cascades,and clinical significance of compensatory upregulation of TX synthase following cycloxygenase-2 inhibition.These obse-rvations underscore the potential need for receptor-targeted therapies,biomarker-guided patient stratification,and improved translational models to inform the development of personalized anti-inflammatory interventions in lung cancer.
基金financially supported by National Natural Science Foundation of China(NSFC,Nos.81873921 and 51903174)Sino-German Cooperation Group Project(No.GZ1512)+2 种基金China Postdoctoral Science Foundation(No.2021M702772)Chengdu Science and Technology Program(No.2020-GH02-00007-HZ)Sichuan University Postdoctoral Interdisciplinary Innovation Startup Foundation and the Fundamental Research Funds for Central Universities(No.2021SCU12070)。
文摘The integrated lipopeptide(RVA)/gene complexes are fabricated with bi-directional regulation on tumor cells and micro-environment.After self-assembling and target coating modification,the poly(γ-glutamic acid)(γ-PGA)/RVA nano-vectors can sequentially respond to pH&redox stimuli,and guarantee efficient therapeutic gene delivery and control release of all-trans retinoic acid.The design provides a facile but promising strategy to treat refractory cancers.
基金supported by the National 973 Basic Research Program of China(No.2013CB733800)the National Natural Science Foundation of China(Nos.11272091 and 11102113)
文摘A three-dimensional （3D） mathematical model of tumour growth at the avascular phase and vessel remodelling in host tissues is proposed with emphasis on the study of the interactions of tumour growth and hypoxic micro-environment in host tissues. The hybrid based model includes the continuum part, such as the distributions of oxygen and vascular endothelial growth factors （VEGFs）, and the discrete part of tumour cells （TCs） and blood vessel networks. The simulation shows the dynamic process of avascular tumour growth from a fewinitial cells to an equilibrium state with varied vessel networks. After a phase of rapidly increasing numbers of the TCs, more and more host vessels collapse due to the stress caused by the growing tumour. In addition, the consumption of oxygen expands with the enlarged tumour region. The study also discusses the effects of certain factors on tumour growth, including the density and configuration of pre- existing vessel networks and the blood oxygen content. The model enables us to examine the relationship between early tumour growth and hypoxic micro-environment in host tissues, which can be useful for further applications, such as tumour metastasis and the initialization of tumour angiogenesis.
文摘Three kinds of packaging bags composited with barrier film and moisture absorbents were designed in order to find out how to create an internal micro-environment with low O2content and relative humidity( RH). Peanuts with different moisture contents were filled into packaging bags and stored in an environmental chamber for a certain period of time. An RH recorder was sealed in each bag and the RH inside the bag was recorded. The moisture contents of peanuts before and after storage were measured as well.It is found that peanuts with moisture content of 13% can reduce O2content inside the bag by the aerobic respiration,achieving the self modified atmosphere packaging( MAP) effect. A microenvironment with lower RH can be created by using super-absorbent fiber( SAF) or mineral bag as the moisture absorbent. The moisture contents of peanuts stored in the bags with SAF and mineral bag as moisture absorbent decreased from 13% to 10. 5%and 11. 3%,respectively. SAF is a promising material of packaging bags composited with barrier film,in which a low RH and high CO2content can be created.
文摘A small English class can be called as a micro-environment for English study. In this small micro-environment, the relationship between teachers and students is subtle. How to achieve the good interaction and create a good micro-environment in English class between teachers and students is very important for developing students＇ learning ability. This thesis is based on Rogers＇ humanistic teaching theory, which uses questionnaires, inteview and comparative analysis to analyze the current situation of micro-environment in college English learning. It discusses the construction of micro-environment in college English learning based on Rogers＇ humanistic teaching theory and the ways to construct it from software conditions （roles of teachers and students, relationship between teachers and students, the classroom atmosphere） and hardware conditions（teaching resources, a classroom）.
基金partially supported by the National Natural Science Foundation of China(No.82473256)the Jiangxi Provincial Natural Science Foundation,China(No.20242BAB25521)+7 种基金Ganpo Promising Talents Supporting Plan—Talent Development Project of Leading Academic and Technological Researchers in Key Disciplines(No.20243BCE51060)the Anhui Province Higher Education Scientific Research Project,China(No.2024AH050818)the Anhui Provincial Natural Science Foundation,China(No.2208085MH251)the Fundamental Research Funds for the Anhui Medical University,China(No.2021xkj131)the Research Fund of Anhui Institute of Translational Medicine,China(No.2023zhyx-C19)the Health Research Program of Anhui,China(No.AHWJ2023A30007)the Anhui Provincial Department of Education,Provincial Quality Engineering Project for Higher Education(Nos.2022jyxm761 and 2023jyxm1106)the Basic and Clinical Cooperative Research and Promotion Program of the Anhui Medical University,China(No.2022xkj T024)。
文摘Cancer is the most common cause of human mortality and has created an unbridgeable health gap due to its unrestrained aberrant proliferation,rapid growth,metastasis,and high heterogeneity.Conventional two-dimensional cell culture and animal models for tumor diagnostic and therapeutic studies have extremely low clinical translation rates due to their intrinsic limitations.Appropriate tumor models are therefore required for cancer research.Engineered human three-dimensional(3D)cancer models stand out for their ability to better replicate the spatial organization,cellular resources,and microenvironmental features(e.g.,hypoxia,necrosis,and delayed proliferation)of actual human tumors.Further,the fabrication of these models can be achieved by an emerging technology known as 3D bioprinting,which allows for the fabrication of living structures by precisely regulating the spatial distribution of cells,biomolecules,and matrix components.The aim of this paper is to review the current technologies and bioinks associated with 3D bioprinted cancer models for glioma,breast,liver,intestinal,cervical,ovarian,and neuroblastoma,as well as,advances in the applications of 3D bioprinted-based tumor models in the fields of tumor microenvironment,tumor vascularization,tumor stem cells,tumor resistance and therapeutic drug screening,tumorimmunotherapy,and precision medicine.
文摘BACKGROUND Colorectal cancer(CRC)prognosis prediction is currently a major challenge.Epigenetic regulation has been widely reported for its role in cancer development.AIM To construct a robust prognostic signature,we used developed and validated across datasets.METHODS After constructing the signature,the prognostic value of the signature was evaluated in the TCGA cohort and six independent datasets(GSE17526,GSE17537,GSE33113,GSE37892,GSE39048 and GSE39582).The clinical,genomic and transcriptomic features related to the signature were identified.The correlations of the signature score with immune cell infiltration and cell-cell interactions were analyzed.The correlations between the signature score and the sensitivity to different drugs were also predicted.RESULTS In the TCGA cohort,patients in the low-risk group according to the signature score had longer survival than those in the high-risk group,and this finding was validated in the validation datasets.The signature was a prognostic factor independent of age and sex and was correlated with stage and PD-1/PD-L1 expression.Area under the receiving operating characteristic curve was 0.72.Genomic association analyses revealed that samples from highrisk patients exhibited chromosomal instability.Transcriptomic analyses revealed that the signature score was significantly associated with multiple cellular pathways.Bulk RNA-seq and single-cell sequencing data revealed that the signature reflected differences in infiltrating immune cell-tumor cell interactions,especially for macrophages.The signature also predicted the putative drug sensitivity of CRC samples.CONCLUSION The signature is a valuable biomarker for predicting CRC prognosis and reflects multiple features of CRC,especially macrophage infiltration in the microenvironment.
基金supported by the National Natural Science Foundation of China (Nos. 21462050 and 21672185)the Foundation of the Department of Science and Technology of Yunnan Province of China (No. 2016FB020)the Program for Excellent Youth Talents (No. C6176102), Yunnan University
文摘Optical sensors are widely used in the field of analytical sensing and optical imaging because of their high sensitivity, fast response time, and technical simplicity. The advantages of sensors lay in their non- invasive approach within biological tissues and inherent reliability in fluorescence imaging tests. In recent years the development of optical sensors for the sensing of ions, neutral molecules and especially tumor micro-environment-related parameters has been an active research area. This review focuses on recent contributions concerning fluorescent or colorimetric sensors and is organized according to their target classifications.
文摘Colorectal cancer(CRC) constitutes the third most frequently reported malignancy in the male population and the second most common in women in the last two decades. Colon carcinogenesis is a complex, multifactorial event, resulting from genetic and epigenetic aberrations, the impact of environmental factors, as well as the disturbance of the gut microbial ecosystem. The relationship between the intestinal microbiome and carcinogenesis was relatively undervalued in the last decade. However, its remarkable effect on metabolic and immune functions on the host has been in the spotlight as of recent years. There is a strong relationship between gut microbiome dysbiosis, bowel pathogenicity and responsiveness to anti-cancer treatment;including immunotherapy. Modifications of bacteriome consistency are closely associated with the immunologic response to immunotherapeutic agents. This condition that implies the necessity of gut microbiome manipulation. Thus, creatingan optimal response for CRC patients to immunotherapeutic agents. In this paper, we will review the current literature observing how gut microbiota influence the response of immunotherapy on CRC patients.
基金support by the Hi-Tech Research and Development Program(863) of China(No.2010AA064902)the National Key Technologies R&D Program(No.2016YFC0207100)+1 种基金the National Natural Science Foundation of China(No.21207116)the Brain Bridge project with Philips(No.BB3-2016-01)
文摘Based on previous research, the sampling and analysis methods for phthalate esters （PAEs） were improved by increasing the sampling flow of indoor air from 1 to 4 L/min, shortening the sampling duration from 8 to 2 hr. Meanwhile, through the optimization of chromatographic conditions, the concentrations of 9 additional PAE pollutants in indoor air were measured. The optimized chromatographic conditions required a similar amount of time for analysis as before, but gave high responsivity, the capability of simultaneously distinguishing 15 kinds of PAEs, and a high level of discrimination between individual sample peaks, as well as stable peak generation. The recovery rate of all gas-phase and particle-phase samples of the 15 kinds of PAEs ranged from 91.26% to 109.42%, meeting the quantitative analysis requirements for indoor and outdoor air sampling and analysis. For the first time, investigation of the concentration levels as well as characteristics of 15 kinds of PAEs in the indoor air from four different traffic micro-environments （private vehicles, busses, taxis and subways） was carried out, along with validation of the optimized sampling and analytical method. The results show that all the 9 additional PAEs could be detected at relatively high pollution levels in the indoor air from the four traffic micro-environments. As none of the pollution levels of the 15 kinds of PAEs in the indoor air from the 4 traffic micro-environments should be neglected, it is of great significance to increase the types of PAEs able to be detected in indoor air.
基金supported by grants from the National Natural Science Foundation of China (No. 81770152, 91642111 and 81570143)the Guangzhou Science and Technology Project (No. 201510010211, 201807010004 and 201803040017)
文摘Human T cells are a highly heterogeneous population and can recognize a wide variety of antigens by their T cell receptors(TCRs). Tumor cells display a large repertoire of antigens that serve as potential targets for recognition,thus making T cells in the tumor micro-environment more complicated. Making a connection between TCRs and the transcriptional information of individual T cells will be interesting for investigating clonal expansion within T cell populations under pathologic conditions. Advances in single cell RNA-sequencing(scRNA-seq) have allowed for comprehensive analysis of T cells. In this review, we briefly describe the research progress on tumor microenvironment T cells using single cell RNA sequencing, and then discuss how scRNA-seq can be used to resolve immune system heterogeneity in health and disease. Finally, we point out future directions in this field and potential for immunotherapy.
基金the National Natural Science Foundation of China(Nos.12002232 and 31870934)the Shanxi Bethune Hospital Hospital Fund(No.2019YJ12)。
文摘Hyperplastic scar is a common fibrotic disease that may ultimately lead to severe dysfunction anddeformity,causing physical and psychological distress.Therefore,we aim to evaluate the effect of the mechanicalmicroenvironment of scar substrates on the morphology of human fibroblasts(HFbs).The micro-modular fabrication technique was used to design a new cross-groove topology and to construct four elastic substrates withthe stiffness of 19.3 kPa and 90.1 kPa coupled with parallel groove and cross groove,respectively,to simulate themechanical microenvironment of skin wounds and scar tissues.The morphological changes in HFbs in differentsubstrates were observed,and the changes in the cell-long axis length,area,and the angle between cell-long axisand grooves were recorded.Immunofluorescence staining was performed to observe the distribution of microfilaments.The results indicated that substrate stiffness and topography affected the morphology of HFbs.The cellswere elongated in parallel grooves as well as in the area where cross grooves restricted groove length,the celllength was restricted,and the angle between the long axis and the groove was increased.The topography exertedno significant effect on the cell area,but the cell area increased with increasing the stiffness.The parallel groovepromoted the expression of the F-actin to a certain extent,and the fluorescence intensity of F-actin decreased withincreasing the stiffness.Studying the effect of the mechanical microenvironment of substrates on HFb morphologyis of great importance for understanding the mechanisms of scar formation and prevention.
