Micro-communities are supposed to have more potential functions of biodegradation of polysaccharides than single strain; however, the intestinal mi ties involved in the biodegradation of Enteromorpha polysaccharides ...Micro-communities are supposed to have more potential functions of biodegradation of polysaccharides than single strain; however, the intestinal mi ties involved in the biodegradation of Enteromorpha polysaccharides （EP） were sel- dom reported. In order to obtain the EP-degrading micro-community, the intestines of Siganus oramin was obtained to isolate the micro-communities, which were enriched by 0.3% of EP as the sole carbon source. A stable micro-community with EP degradative capability was achieved after seven generations of subculture, named H1. Results showed that H1 was able to degrade 75% of EP within 24 hours, and the activity of EP lyases reached 500 U mL-1 in 32 hours. With denaturing gradient gel electrophoresis （DGGE） and 16S rRNA gene clone library analysis, ten bacteria closely related to Marinomonas pontica, Microbacterium sp., Leucobacter chironomi, Cyclobacterium sp., Algoriphagus winogradskyi, Pseudoalteromonas sp. and Vibrio sp. were determined. Furthermore, compared with the DGGE bands sequence and the clone library analysis, the dominant bacteria of the EP-biodegrading mi- cro-community were Pseudoalteromonas sp. and Vibrio sp., with the respective proportion of 38% and 46%, and they should play an important role in EP degradation together with other degrading bacteria in the micro-community H1.展开更多
Thermostatically controlled loads(TCLs)on the demand side have been a vital energy resource in smart grids.To efficiently utilize the large-scale TCLs and enhance the flexibility of micro-community systems,this paper ...Thermostatically controlled loads(TCLs)on the demand side have been a vital energy resource in smart grids.To efficiently utilize the large-scale TCLs and enhance the flexibility of micro-community systems,this paper proposes a distributed coordinated control strategy based on the distributed model predictive control(MPC).To achieve the adaptive coordinated control among TCLs and consider user comfort constraints,a distributed dual-layer internal control strategy based on MPC is established on a scalable communication network.This strategy achieves the efficient utilization of TCLs in a distributed manner and notably improves the convergence speed through sparse network communication between neighbors.For external resource utilization of TCLs,a multi-timescale scheduling framework is proposed to realize the pre-allocation of electricity.Furthermore,the feasibility of the proposed distributed coordinated control strategy is confirmed through comparative case analysis.展开更多
基金supported by the National Natural Science Foundation of China (Nos.41476150 and 41276179)Guangdong Natural Science Foundation (No.S2011030005257)the Science and Technology Project of Guangdong Province (Nos.2012A031100009 and 2012B060400016)
文摘Micro-communities are supposed to have more potential functions of biodegradation of polysaccharides than single strain; however, the intestinal mi ties involved in the biodegradation of Enteromorpha polysaccharides （EP） were sel- dom reported. In order to obtain the EP-degrading micro-community, the intestines of Siganus oramin was obtained to isolate the micro-communities, which were enriched by 0.3% of EP as the sole carbon source. A stable micro-community with EP degradative capability was achieved after seven generations of subculture, named H1. Results showed that H1 was able to degrade 75% of EP within 24 hours, and the activity of EP lyases reached 500 U mL-1 in 32 hours. With denaturing gradient gel electrophoresis （DGGE） and 16S rRNA gene clone library analysis, ten bacteria closely related to Marinomonas pontica, Microbacterium sp., Leucobacter chironomi, Cyclobacterium sp., Algoriphagus winogradskyi, Pseudoalteromonas sp. and Vibrio sp. were determined. Furthermore, compared with the DGGE bands sequence and the clone library analysis, the dominant bacteria of the EP-biodegrading mi- cro-community were Pseudoalteromonas sp. and Vibrio sp., with the respective proportion of 38% and 46%, and they should play an important role in EP degradation together with other degrading bacteria in the micro-community H1.
基金supported in part by the National Key Research and Development Program of China(No.2023YFB2406600)the Joint Funds of the National Natural Science Foundation of China(No.U22A6007)the National Science Fund for Excellent Young Scholars of China(No.52222703).
文摘Thermostatically controlled loads(TCLs)on the demand side have been a vital energy resource in smart grids.To efficiently utilize the large-scale TCLs and enhance the flexibility of micro-community systems,this paper proposes a distributed coordinated control strategy based on the distributed model predictive control(MPC).To achieve the adaptive coordinated control among TCLs and consider user comfort constraints,a distributed dual-layer internal control strategy based on MPC is established on a scalable communication network.This strategy achieves the efficient utilization of TCLs in a distributed manner and notably improves the convergence speed through sparse network communication between neighbors.For external resource utilization of TCLs,a multi-timescale scheduling framework is proposed to realize the pre-allocation of electricity.Furthermore,the feasibility of the proposed distributed coordinated control strategy is confirmed through comparative case analysis.
