MicroRNAs(miRNAs) are a class of small endogenous non-coding RNAs that direct post-transcriptional gene silencing.In plants,numerous miRNAs have been demonstrated to be regulated under drought-induced stress.However,t...MicroRNAs(miRNAs) are a class of small endogenous non-coding RNAs that direct post-transcriptional gene silencing.In plants,numerous miRNAs have been demonstrated to be regulated under drought-induced stress.However,the role of miRNAs in drought regulation remains unclear in potato.In this work,the function of stu-miR159a was investigated in responding to drought stress in potato.Upon examination,StGAMyb-like1 was identified as the target gene for stu-miR159a.Overexpression of stu-miR159a(stu-miR159a OE plants) increased sensitivity to drought,interference with stu-miR159a activity by target mimics(stu-miR159a ST plants) resulted in drought resistance.During drought treatment,the target gene StGAMyb-like1 showed increased activation in stu-miR159a ST plants compared to non-transgenic plants.In contrast,drought stress induced weaker activation of the target gene in stu-miR159a OE plants.In stu-miR159a ST plants,the expression of critical genes in the anthocyanin biosynthesis pathway(StF3'5'H,StF3'H and StCHS2)was increased by decreasing stu-miR159a activity and simultaneously increasing that of StGAMyb-like1.Meanwhile,with drought treatment,stu-miR159a ST plants exhibited higher anthocyanin accumulation than non-transgenic ones,indicating enhanced antioxidant capacity and improved drought tolerance.The above data support that stu-miR159a is a negative regulator of drought stress and provide new insights into the stu-miR159a-mediated regulation of the anthocyanin biosynthesis pathway in controlling drought tolerance in potato.展开更多
Objective: To verify the existence of microRNAs(miRNAs) extracted from fresh ginseng decoction.Methods: Fresh ginseng was prepared into decoction according to the conventional method. The miRNA were extracted from the...Objective: To verify the existence of microRNAs(miRNAs) extracted from fresh ginseng decoction.Methods: Fresh ginseng was prepared into decoction according to the conventional method. The miRNA were extracted from the condensed ginseng decoction by plant microRNA extraction kit. Then miRNA were treated by DNase I and subjected to agarose gel electrophoresis and Agilent 2100 bioanalysis. Mi R-159 and mi R-6135, which were highly expressed in ginseng, were selected and verified by real-time quantitative PCR to detect the expression in the decoction.Results: Ginseng miRNA were successfully extracted from fresh decoction. Mi R-159 and mi R-6135 were expressed in fresh decoction with lower levels than those of fresh ginseng.Conclusion: miRNAs stably existed after processing, and retained some stability after high-temperature treatment. The findings provide a valuables basis for the further studies on ginseng miRNAs.展开更多
Fusarium wilt,caused by the soil-borne fungal pathogen,Fusarium oxysporum f.sp.cubense(Foc),is considered as one of the most threatening diseases of banana.The Cavendish variety,resistant to Foc race 1(R1),is suscepti...Fusarium wilt,caused by the soil-borne fungal pathogen,Fusarium oxysporum f.sp.cubense(Foc),is considered as one of the most threatening diseases of banana.The Cavendish variety,resistant to Foc race 1(R1),is susceptible to tropical race 4(TR4),an aggressive race of the pathogen which is of increasing concern worldwide.Previous studies have revealed that plant small RNAs(sRNAs)play crucial roles in the host response to pathogen infection.To investigate the roles of sRNAs involved in the interaction of the banana-Foc pathosystem,small RNA profiles of Cavendish banana roots inoculated with Foc TR4 and Foc R1 were obtained and analyzed in the present study using Next-Generation Sequencing(NGS)technology.A total of 112 discrete mature microRNAs(miRNAs)belonging to 22 known miRNA families were found across all constructed sRNA libraries.The expression of miR166,miR159 and miR156 was upregulated in TR4-inoculated samples as compared to mock-inoculated samples,while the expression of these miRNAs was approximately the same in R1-inoculated and mock-inoculated samples.Consistent with the sequencing data,qRT-PCR results demonstrated up-regulation of these miRNAs and down-regulation of their target genes in TR4-inoculated samples,but not in R1-inoculated samples.Considering Cavendish banana is resistant to R1 and susceptible to TR4,it is possible that these sRNAs and their target genes are involved in particular plant defence pathways such as salicylic acid-based defence.The findings will pave way for future investigations of the defence mechanism and potential approaches of resistance improvement.展开更多
基金supported by the Gansu Science and Technology Major Project(Grant No.22ZD6NA009)the Key Program of Natural Science Foundation of Gansu Province(Grant No.22JR5RA832)+1 种基金the Gansu Science and Technology Major Project(Grant No.23ZDNA006)National Natural Science Foundation of China(Grant No.31860399).
文摘MicroRNAs(miRNAs) are a class of small endogenous non-coding RNAs that direct post-transcriptional gene silencing.In plants,numerous miRNAs have been demonstrated to be regulated under drought-induced stress.However,the role of miRNAs in drought regulation remains unclear in potato.In this work,the function of stu-miR159a was investigated in responding to drought stress in potato.Upon examination,StGAMyb-like1 was identified as the target gene for stu-miR159a.Overexpression of stu-miR159a(stu-miR159a OE plants) increased sensitivity to drought,interference with stu-miR159a activity by target mimics(stu-miR159a ST plants) resulted in drought resistance.During drought treatment,the target gene StGAMyb-like1 showed increased activation in stu-miR159a ST plants compared to non-transgenic plants.In contrast,drought stress induced weaker activation of the target gene in stu-miR159a OE plants.In stu-miR159a ST plants,the expression of critical genes in the anthocyanin biosynthesis pathway(StF3'5'H,StF3'H and StCHS2)was increased by decreasing stu-miR159a activity and simultaneously increasing that of StGAMyb-like1.Meanwhile,with drought treatment,stu-miR159a ST plants exhibited higher anthocyanin accumulation than non-transgenic ones,indicating enhanced antioxidant capacity and improved drought tolerance.The above data support that stu-miR159a is a negative regulator of drought stress and provide new insights into the stu-miR159a-mediated regulation of the anthocyanin biosynthesis pathway in controlling drought tolerance in potato.
基金supported by the National Natural Science Foundation for Young Scholars of China(no.81403195)Natural Science Foundation of Guangdong Province(no.S2013010015418)
文摘Objective: To verify the existence of microRNAs(miRNAs) extracted from fresh ginseng decoction.Methods: Fresh ginseng was prepared into decoction according to the conventional method. The miRNA were extracted from the condensed ginseng decoction by plant microRNA extraction kit. Then miRNA were treated by DNase I and subjected to agarose gel electrophoresis and Agilent 2100 bioanalysis. Mi R-159 and mi R-6135, which were highly expressed in ginseng, were selected and verified by real-time quantitative PCR to detect the expression in the decoction.Results: Ginseng miRNA were successfully extracted from fresh decoction. Mi R-159 and mi R-6135 were expressed in fresh decoction with lower levels than those of fresh ginseng.Conclusion: miRNAs stably existed after processing, and retained some stability after high-temperature treatment. The findings provide a valuables basis for the further studies on ginseng miRNAs.
基金funded by the Accelerated Partnership Grant,Queensland Government(2014000652)Shulang Fei’s PhD programme is supported by the China Scholarship Council and the University of Queensland。
文摘Fusarium wilt,caused by the soil-borne fungal pathogen,Fusarium oxysporum f.sp.cubense(Foc),is considered as one of the most threatening diseases of banana.The Cavendish variety,resistant to Foc race 1(R1),is susceptible to tropical race 4(TR4),an aggressive race of the pathogen which is of increasing concern worldwide.Previous studies have revealed that plant small RNAs(sRNAs)play crucial roles in the host response to pathogen infection.To investigate the roles of sRNAs involved in the interaction of the banana-Foc pathosystem,small RNA profiles of Cavendish banana roots inoculated with Foc TR4 and Foc R1 were obtained and analyzed in the present study using Next-Generation Sequencing(NGS)technology.A total of 112 discrete mature microRNAs(miRNAs)belonging to 22 known miRNA families were found across all constructed sRNA libraries.The expression of miR166,miR159 and miR156 was upregulated in TR4-inoculated samples as compared to mock-inoculated samples,while the expression of these miRNAs was approximately the same in R1-inoculated and mock-inoculated samples.Consistent with the sequencing data,qRT-PCR results demonstrated up-regulation of these miRNAs and down-regulation of their target genes in TR4-inoculated samples,but not in R1-inoculated samples.Considering Cavendish banana is resistant to R1 and susceptible to TR4,it is possible that these sRNAs and their target genes are involved in particular plant defence pathways such as salicylic acid-based defence.The findings will pave way for future investigations of the defence mechanism and potential approaches of resistance improvement.
