MicroRNAs(miRNAs) are a class of small endogenous non-coding RNAs that direct post-transcriptional gene silencing.In plants,numerous miRNAs have been demonstrated to be regulated under drought-induced stress.However,t...MicroRNAs(miRNAs) are a class of small endogenous non-coding RNAs that direct post-transcriptional gene silencing.In plants,numerous miRNAs have been demonstrated to be regulated under drought-induced stress.However,the role of miRNAs in drought regulation remains unclear in potato.In this work,the function of stu-miR159a was investigated in responding to drought stress in potato.Upon examination,StGAMyb-like1 was identified as the target gene for stu-miR159a.Overexpression of stu-miR159a(stu-miR159a OE plants) increased sensitivity to drought,interference with stu-miR159a activity by target mimics(stu-miR159a ST plants) resulted in drought resistance.During drought treatment,the target gene StGAMyb-like1 showed increased activation in stu-miR159a ST plants compared to non-transgenic plants.In contrast,drought stress induced weaker activation of the target gene in stu-miR159a OE plants.In stu-miR159a ST plants,the expression of critical genes in the anthocyanin biosynthesis pathway(StF3'5'H,StF3'H and StCHS2)was increased by decreasing stu-miR159a activity and simultaneously increasing that of StGAMyb-like1.Meanwhile,with drought treatment,stu-miR159a ST plants exhibited higher anthocyanin accumulation than non-transgenic ones,indicating enhanced antioxidant capacity and improved drought tolerance.The above data support that stu-miR159a is a negative regulator of drought stress and provide new insights into the stu-miR159a-mediated regulation of the anthocyanin biosynthesis pathway in controlling drought tolerance in potato.展开更多
Objective: To verify the existence of microRNAs(miRNAs) extracted from fresh ginseng decoction.Methods: Fresh ginseng was prepared into decoction according to the conventional method. The miRNA were extracted from the...Objective: To verify the existence of microRNAs(miRNAs) extracted from fresh ginseng decoction.Methods: Fresh ginseng was prepared into decoction according to the conventional method. The miRNA were extracted from the condensed ginseng decoction by plant microRNA extraction kit. Then miRNA were treated by DNase I and subjected to agarose gel electrophoresis and Agilent 2100 bioanalysis. Mi R-159 and mi R-6135, which were highly expressed in ginseng, were selected and verified by real-time quantitative PCR to detect the expression in the decoction.Results: Ginseng miRNA were successfully extracted from fresh decoction. Mi R-159 and mi R-6135 were expressed in fresh decoction with lower levels than those of fresh ginseng.Conclusion: miRNAs stably existed after processing, and retained some stability after high-temperature treatment. The findings provide a valuables basis for the further studies on ginseng miRNAs.展开更多
基金supported by the Gansu Science and Technology Major Project(Grant No.22ZD6NA009)the Key Program of Natural Science Foundation of Gansu Province(Grant No.22JR5RA832)+1 种基金the Gansu Science and Technology Major Project(Grant No.23ZDNA006)National Natural Science Foundation of China(Grant No.31860399).
文摘MicroRNAs(miRNAs) are a class of small endogenous non-coding RNAs that direct post-transcriptional gene silencing.In plants,numerous miRNAs have been demonstrated to be regulated under drought-induced stress.However,the role of miRNAs in drought regulation remains unclear in potato.In this work,the function of stu-miR159a was investigated in responding to drought stress in potato.Upon examination,StGAMyb-like1 was identified as the target gene for stu-miR159a.Overexpression of stu-miR159a(stu-miR159a OE plants) increased sensitivity to drought,interference with stu-miR159a activity by target mimics(stu-miR159a ST plants) resulted in drought resistance.During drought treatment,the target gene StGAMyb-like1 showed increased activation in stu-miR159a ST plants compared to non-transgenic plants.In contrast,drought stress induced weaker activation of the target gene in stu-miR159a OE plants.In stu-miR159a ST plants,the expression of critical genes in the anthocyanin biosynthesis pathway(StF3'5'H,StF3'H and StCHS2)was increased by decreasing stu-miR159a activity and simultaneously increasing that of StGAMyb-like1.Meanwhile,with drought treatment,stu-miR159a ST plants exhibited higher anthocyanin accumulation than non-transgenic ones,indicating enhanced antioxidant capacity and improved drought tolerance.The above data support that stu-miR159a is a negative regulator of drought stress and provide new insights into the stu-miR159a-mediated regulation of the anthocyanin biosynthesis pathway in controlling drought tolerance in potato.
基金supported by the National Natural Science Foundation for Young Scholars of China(no.81403195)Natural Science Foundation of Guangdong Province(no.S2013010015418)
文摘Objective: To verify the existence of microRNAs(miRNAs) extracted from fresh ginseng decoction.Methods: Fresh ginseng was prepared into decoction according to the conventional method. The miRNA were extracted from the condensed ginseng decoction by plant microRNA extraction kit. Then miRNA were treated by DNase I and subjected to agarose gel electrophoresis and Agilent 2100 bioanalysis. Mi R-159 and mi R-6135, which were highly expressed in ginseng, were selected and verified by real-time quantitative PCR to detect the expression in the decoction.Results: Ginseng miRNA were successfully extracted from fresh decoction. Mi R-159 and mi R-6135 were expressed in fresh decoction with lower levels than those of fresh ginseng.Conclusion: miRNAs stably existed after processing, and retained some stability after high-temperature treatment. The findings provide a valuables basis for the further studies on ginseng miRNAs.
