Background Circular RNAs(circRNAs)participate in the development of periodontitis.The present work aims to reveal the role and mechanism of circ_0087199 in human periodontal ligament cell(PDLC)injury during periodonti...Background Circular RNAs(circRNAs)participate in the development of periodontitis.The present work aims to reveal the role and mechanism of circ_0087199 in human periodontal ligament cell(PDLC)injury during periodontitis.Methods PDLCs were treated with lipopolysaccharides(LPS)to establish a periodontitis cell model.Quantitative real-time polymerase chain reaction was used to detect the expression of circ_0087199,miR-527,toll-like receptor 4(TLR4).Western blotting assay was performed to assess protein expression.Cell viability,proliferation,apoptosis and inflammation were investigated by cell counting kit-8,EdU assay,flow cytometry and enzyme-linked immunosorbent assay,respectively.Oxidative stress was evaluated by malondialdehyde assay kit and superoxide dismutase activity assay kit.The interaction between miR-527 and circ_0087199 or TLR4 was confirmed by a dual-luciferase reporter assay.Results Circ_0087199 and TLR4 expression levels were significantly increased,while miR-527 was decreased in the periodontal ligament tissues of periodontitis patients and LPS-stimulated PDLCs when compared with controls.LPS treatment inhibited cell viability and proliferation but induced cell apoptosis,inflammation and oxidative stress,whereas these effects were attenuated after circ_0087199 knockdown.Circ_0087199 bound to miR-527 and regulated LPS-caused PDLC damage by targeting miR-527.Additionally,the overexpression of TLR4,a target gene of miR-527,rescued miR-527 mimic-mediated effects on LPS-treated PDLCs.Further,the regulation of circ_0087199 toward TLR4 involved miR-527.Conclusion Circ_0087199 knockdown attenuated LPS-induced apoptosis,inflammation and oxidative stress of PDLCs by regulating the miR-527/TLR4 pathway.展开更多
基金supported by the Key Special Projects of National Key R&D Plan (2022YFC3103202)
文摘Background Circular RNAs(circRNAs)participate in the development of periodontitis.The present work aims to reveal the role and mechanism of circ_0087199 in human periodontal ligament cell(PDLC)injury during periodontitis.Methods PDLCs were treated with lipopolysaccharides(LPS)to establish a periodontitis cell model.Quantitative real-time polymerase chain reaction was used to detect the expression of circ_0087199,miR-527,toll-like receptor 4(TLR4).Western blotting assay was performed to assess protein expression.Cell viability,proliferation,apoptosis and inflammation were investigated by cell counting kit-8,EdU assay,flow cytometry and enzyme-linked immunosorbent assay,respectively.Oxidative stress was evaluated by malondialdehyde assay kit and superoxide dismutase activity assay kit.The interaction between miR-527 and circ_0087199 or TLR4 was confirmed by a dual-luciferase reporter assay.Results Circ_0087199 and TLR4 expression levels were significantly increased,while miR-527 was decreased in the periodontal ligament tissues of periodontitis patients and LPS-stimulated PDLCs when compared with controls.LPS treatment inhibited cell viability and proliferation but induced cell apoptosis,inflammation and oxidative stress,whereas these effects were attenuated after circ_0087199 knockdown.Circ_0087199 bound to miR-527 and regulated LPS-caused PDLC damage by targeting miR-527.Additionally,the overexpression of TLR4,a target gene of miR-527,rescued miR-527 mimic-mediated effects on LPS-treated PDLCs.Further,the regulation of circ_0087199 toward TLR4 involved miR-527.Conclusion Circ_0087199 knockdown attenuated LPS-induced apoptosis,inflammation and oxidative stress of PDLCs by regulating the miR-527/TLR4 pathway.
