Bacillus species have been widely used as probiotics in a number of food products.However,these are vulnerable to bacteriophage infections,which poses fermentation failure and eventually result in economic losses.Give...Bacillus species have been widely used as probiotics in a number of food products.However,these are vulnerable to bacteriophage infections,which poses fermentation failure and eventually result in economic losses.Given this,we designed this study in which the bacteriophage of lysogenic Bacillus methylotrophicus,phage BM-P1,was induced successfully,and its biological characteristics and genome information were researched.The obtained results showed that phage BM-P1 belonged to Myoviridae family.The maximum number of infections for this phage was 10,with a burst size of 104.48±2.70 counts expressed per milliter per infective center.Additionally,this phage was temperature-and pH-sensitive and divalent ions(Ca2+,Mg2+)and chloramphenicol did not have a significant influence on its adsorption capacity.Genomic analysis revealed that the genome size of phage BM-P1 was 153087 bp with 41.94%GC content,including 258 coding sequences.Furthermore,52 bac-teriophages of Bacillus were classified into two clades by using phylogenetic analysis.Among them,phage BM-P1 with phage VB_BsuM-Goe3 and vB_BveM-Goe7 had the highest average nucleotide identity values,95.23%and 95.28%,respectively.However,synteny analysis revealed transposition,deletion,and insertion in the genome of BM-P1.Considering this,it could be extrapolated that this phage is a new lysogenic phage.In conclusion,this study furthers the knowledge on the properties of B.methylotrophicus phages and provides seminal insights for designingeffective antiphage strategiesforfermentation industry.展开更多
With Fusarium oxysporum f.sp. phaseoli as the target bacterium,the bacterial strain with strong antagonistic activity was screened from crop rhizosphere soil using plate confrontation method and pot efficiency test. ...With Fusarium oxysporum f.sp. phaseoli as the target bacterium,the bacterial strain with strong antagonistic activity was screened from crop rhizosphere soil using plate confrontation method and pot efficiency test. This strain was identified according to morphological observation, physiological and biochemical characteristics and sequence analysis of 16S rRNA gene and gyrB gene. The results showed that LZF-5-3 strain was identified as Bacillus methylotrophicus and the control effect of LZF-5-3 against F.oxysporum in snap bean was 71.42% in pot test.展开更多
基金supported by the Natural Science Foundation of The Department of Food Science and Engineering,Inner Mongolia Agricultural University,China(No.SPKJ202006)the Natural Science Foundation of China(Nos.32160550 and 31760447)+2 种基金the Central-directed Local Projects,China(No.2021ZY0022)the Natural Science Foundation of Inner Mongolia,China(No.2021MS03014)the Inner Mongolia Autonomous Region Science and Technology Project,China(No.2021GG0080).
文摘Bacillus species have been widely used as probiotics in a number of food products.However,these are vulnerable to bacteriophage infections,which poses fermentation failure and eventually result in economic losses.Given this,we designed this study in which the bacteriophage of lysogenic Bacillus methylotrophicus,phage BM-P1,was induced successfully,and its biological characteristics and genome information were researched.The obtained results showed that phage BM-P1 belonged to Myoviridae family.The maximum number of infections for this phage was 10,with a burst size of 104.48±2.70 counts expressed per milliter per infective center.Additionally,this phage was temperature-and pH-sensitive and divalent ions(Ca2+,Mg2+)and chloramphenicol did not have a significant influence on its adsorption capacity.Genomic analysis revealed that the genome size of phage BM-P1 was 153087 bp with 41.94%GC content,including 258 coding sequences.Furthermore,52 bac-teriophages of Bacillus were classified into two clades by using phylogenetic analysis.Among them,phage BM-P1 with phage VB_BsuM-Goe3 and vB_BveM-Goe7 had the highest average nucleotide identity values,95.23%and 95.28%,respectively.However,synteny analysis revealed transposition,deletion,and insertion in the genome of BM-P1.Considering this,it could be extrapolated that this phage is a new lysogenic phage.In conclusion,this study furthers the knowledge on the properties of B.methylotrophicus phages and provides seminal insights for designingeffective antiphage strategiesforfermentation industry.
文摘With Fusarium oxysporum f.sp. phaseoli as the target bacterium,the bacterial strain with strong antagonistic activity was screened from crop rhizosphere soil using plate confrontation method and pot efficiency test. This strain was identified according to morphological observation, physiological and biochemical characteristics and sequence analysis of 16S rRNA gene and gyrB gene. The results showed that LZF-5-3 strain was identified as Bacillus methylotrophicus and the control effect of LZF-5-3 against F.oxysporum in snap bean was 71.42% in pot test.
