Due to various reasons, the inspection methods often need to be changed, and the detection reagents often need to be replaced. In this study, a comparative experiment was conducted between the ethanol-based and ether-...Due to various reasons, the inspection methods often need to be changed, and the detection reagents often need to be replaced. In this study, a comparative experiment was conducted between the ethanol-based and ether-based determination methods for oil content in imported wool. The determination results obtained from the two methods were treated as abscissa and ordinate respectively,and their linear relationship was analyzed. According to the linear regression analysis, the conversion equation of determination result between the two methods was obtained. In addition, the repeatability admissible error and reproducibility admissible error were established through analyzing the comparative experimental results by scientific software. This study will bring new ideas for further researches in this field, and provide reference for solving the similar problems in actual inspection work.展开更多
The developed technique of diffusive gradients in thin film( DGT) has been suggested as a promising tool for evaluation of cadmium( Cd) availability in soils,but there exists considerable debate on its suitability. In...The developed technique of diffusive gradients in thin film( DGT) has been suggested as a promising tool for evaluation of cadmium( Cd) availability in soils,but there exists considerable debate on its suitability. In this study,Cd bioavailability in soils was systemically investigated by comparing this technique with seven traditional methods, including soil solution concentration and six commonly used extraction methods( HAc,EDTA,Na c,NH4Ac,CaCl2 and MgCl2). Two typical plants( wheat and maize) were examined for Cd uptake. Maize was more sensitive to increasing exposure to Cd in soils than wheat when the added amounts of Cd ranged from 3. 5 to 5. 0 mg · kg-1,accompanied by the significant decreases of shoot and root biomasses. Cd concentrations in shoots and roots of two plants increased continuously with increasing levels of Cd in soils. Cd uptake was higher in wheat than that in maize. The bioavailable concentrations of Cd measured by all methods increased nearly linearly with increasing addition of Cd in soils. Positive correlations were observed between various bioavailable indicators of Cd in soils and Cd concentrations in two plants. The extractable Cd by six chemical extractants was considerably higher for maize than for wheat,while the DGT-measured and soil solution concentrations of Cd were lower for maize than for wheat,following the same trend as plant Cd uptake. The results imply that DGT measurement can effectively predict the bioavailable levels of Cd in soil solutions and that it is an ideal tool for prediction of Cd bioavailability in soils.展开更多
Modal parameters can accurately characterize the structural dynamic properties and assess the physical state of the structure.Therefore,it is particularly significant to identify the structural modal parameters accordi...Modal parameters can accurately characterize the structural dynamic properties and assess the physical state of the structure.Therefore,it is particularly significant to identify the structural modal parameters according to the monitoring data information in the structural health monitoring(SHM)system,so as to provide a scientific basis for structural damage identification and dynamic model modification.In view of this,this paper reviews methods for identifying structural modal parameters under environmental excitation and briefly describes how to identify structural damages based on the derived modal parameters.The paper primarily introduces data-driven modal parameter recognition methods(e.g.,time-domain,frequency-domain,and time-frequency-domain methods,etc.),briefly describes damage identification methods based on the variations of modal parameters(e.g.,natural frequency,modal shapes,and curvature modal shapes,etc.)and modal validation methods(e.g.,Stability Diagram and Modal Assurance Criterion,etc.).The current status of the application of artificial intelligence(AI)methods in the direction of modal parameter recognition and damage identification is further discussed.Based on the pre-vious analysis,the main development trends of structural modal parameter recognition and damage identification methods are given to provide scientific references for the optimized design and functional upgrading of SHM systems.展开更多
Effects of different enucleation methods on developmental potency of pig handmade clone（HMC）reconstructed embryo was investigated in the paper.We compared enucleation efficiency of blind cut method,first polar body...Effects of different enucleation methods on developmental potency of pig handmade clone（HMC）reconstructed embryo was investigated in the paper.We compared enucleation efficiency of blind cut method,first polar body（Pb1）positioning method and demecolcine（DM）assisted enucleation,as well as their effects on development of HMC reconstructed embryos.The results showed that overall enucleation efficiency of Pb1 positioning method was significantly higher than that of blind cut method（P〈0.05）.The protuberance rate and overall enucleation efficiency of 0.4μg/mL DM treated group for 60 min was significantly higher than that of other concentrations and time treatment groups（P〈0.05）.For effects on development of HMC reconstructed embryos,there was no significant difference between DM-assisted enucleation and Pb1 positioning method.In conclusion,appropriate addition of DM could enhance enucleation efficiency of HMC,which had no significant influence on developmental potency of reconstructed embryos.展开更多
First, the high-rise building structure design process is divided into three relevant steps, that is, scheme generation and creation, performance evaluation, and scheme optimization. Then with the application of relat...First, the high-rise building structure design process is divided into three relevant steps, that is, scheme generation and creation, performance evaluation, and scheme optimization. Then with the application of relational database, the case database of high-rise structures is constructed, the structure form-selection designing methods such as the smart algorithm based on CBR, DM, FINS, NN and GA is presented, and the original forms system of this method and its general structure are given. CBR and DM are used to generate scheme candidates; FINS and NN to evaluate and optimize the scheme performance; GA to create new structure forms. Finally, the application cases are presented, whose results fit in with the real project. It proves by combining and using the expert intelligence, algorithm intelligence and machine intelligence that this method makes good use of not only the engineering project knowledge and expertise but also much deeper knowledge contained in various engineering cases. In other words, it is because the form selection has a strong background support of vast real cases that its results prove more reliable and more acceptable. So the introduction of this method provides an effective approach to improving the quality, efficiency, automatic and smart level of high-rise structures form selection design.展开更多
目的:研究低氧状态下自噬对2型糖尿病(Diabetes mellitus type 2, T2DM)合并股骨骨折大鼠模型不同时间点的骨折愈合程度及低氧诱导因子-1α (HIF-1α)、碱性磷酸酶(alkaline phosphatase, ALP)、Runt-相关转录因子-2 (Runx2)、微管相关...目的:研究低氧状态下自噬对2型糖尿病(Diabetes mellitus type 2, T2DM)合并股骨骨折大鼠模型不同时间点的骨折愈合程度及低氧诱导因子-1α (HIF-1α)、碱性磷酸酶(alkaline phosphatase, ALP)、Runt-相关转录因子-2 (Runx2)、微管相关蛋白1A/1B-轻链3 (LC3II/I)表达情况。方法:大鼠随机分为高糖高脂饲养前(Control)组、高糖高脂饲养8周后(HFD)组及T2DM模型(T2DM)组,采用高糖髙脂饲料联合链脲佐菌素制备T2DM模型,在T2DM模型基础上制作股骨骨折模型。实验大鼠分为对照组(T2DM合并股骨骨折模型组,Model)及治疗组(氯化钴(CoCl2)治疗的T2DM合并股骨骨折模型组,Treatment),根据检测时间点每组继续分为造模后7 d、28 d、42 d的三个亚组。X线评估股骨愈合情况,WB检测HIF-1α、ALP、Runx2、LC3II/I表达。结果:T2DM合并股骨骨折模型随时间的增长,治疗组在第28 d相比于对照组已有较好的恢复,骨组织中ALP、Runx2表达增加,自噬蛋白LC3II/I的检测结果显示随时间增长自噬水平有所减弱。结论:低氧可以诱导自噬来促进T2DM大鼠骨折愈合。Objective: To investigate fracture healing and hypoxia-inducible factor-1α (HIF-1α), alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), and microtubule associated protein-II/I (LC3II/I) expression at different time points in T2DM rats with femoral fracture under hypoxic conditions. Methods: The rats were randomly divided into high-sugar and high-fat pre-feeding groups, high-sugar and high-fat feeding groups after 8 weeks of feeding and a T2DM model group, T2DM was prepared with high-glucose and high-fat diet combined with streptavidin. Femoral fractures were modeled on the basis of T2DM. Experimental rats were divided into a control group (T2DM with femoral fracture model group, Model) and a treatment group (CoCl2 treatment group, Treatment) and continued to be divided into three subgroups on days 7, 28, and 42 post-molecularization for each group at the time of measurement. Femoral healing was assessed by X-ray. HIF-1α, ALP, Runx2, and LC3II/I expression was measured by Western blotting. Result: In the T2DM combined femoral fracture model, as time progresses, the treatment group shows better recovery compared to the control group at day 28. The expression of ALP and Runx2 in bone tissue increases, and the detection results of autophagy protein LC3II/I indicate that autophagy levels weaken over time. Conclusion: Hypoxia can induce autophagy to promote fracture healing in T2DM rats.展开更多
According to the World Health Organization(WHO),cancer is the leading cause of death for children in low and middle-income countries.Around 400,000 kids get diagnosed with this illness each year,and their survival rat...According to the World Health Organization(WHO),cancer is the leading cause of death for children in low and middle-income countries.Around 400,000 kids get diagnosed with this illness each year,and their survival rate depends on the country in which they live.In this article,we present a Pythagorean fuzzy model that may help doctors identify the most likely type of cancer in children at an early stage by taking into account the symptoms of different types of cancer.The Pythagorean fuzzy decision-making techniques that we utilize are Pythagorean Fuzzy TOPSIS,Pythagorean Fuzzy Entropy(PF-Entropy),and Pythagorean Fuzzy PowerWeighted Geometric(PFPWG).Ourmodel is fed with nineteen symptoms and it diagnoses the risk of eight types of cancers in children.We develop an algorithm for each method and calculate its complexity.Additionally,we consider an example to make a clear understanding of our model.We also compare the final results of various tests that prove the authenticity of this study.展开更多
光伏发电系统的发电效率与最大功率点的捕捉与跟踪技术密切相关。当辐照度、温度及其他外部环境变化时,光伏电池的开路电压、短路电流和最大功率点电压及电流也随之变化。为提高光伏发电效率,寻求光伏电池的最优工作状态,大多数的最大...光伏发电系统的发电效率与最大功率点的捕捉与跟踪技术密切相关。当辐照度、温度及其他外部环境变化时,光伏电池的开路电压、短路电流和最大功率点电压及电流也随之变化。为提高光伏发电效率,寻求光伏电池的最优工作状态,大多数的最大功率点跟踪(Maximum power point tracking,MPPT)主要作用于DC-DC变换器的输入端,该方式在MPPT时会受到变换器损耗的影响,降低最大功率点寻优精度。基于Boost电路,在DC-DC变换器的输出端搭建MPPT系统,通过调节输出电压参数来实现对效果变化的控制,且在MPPT系统引入了导纳微分法(Admittance differentiation method,ADM),实现对MPPT控制器的重构,实施中搭建不同的仿真模型,再借助仿真模型达到对ADM的修正与优化,综合论证该方法具有更优的指标和更好的效果。展开更多
Minocycline has been widely used in clinical treatment and its residues were considered to have environmental safety risks due to complex chemical structure.Therefore,it is necessary to find an efficient and environme...Minocycline has been widely used in clinical treatment and its residues were considered to have environmental safety risks due to complex chemical structure.Therefore,it is necessary to find an efficient and environmentally friendly method to remove minocycline from the environment.This study screened and isolated a minocycline degrading strain DM13 from the activated sludge for municipal sewage pipeline,and optimized the biodegradation of minocycline by DM13 under various environmental conditions.The maximum biodegradation efficiency of 50 mg/L minocycline reached 93%at 72 h with the temperature of 30℃,the initial pH of 7.0,and the inoculation rate of 3%.Two potential biotransformation pathways were proposed,including deamination,demethylation,and decarbonylation.The acute toxicity assessment showed that the biotransformation products of minocycline had lower toxicity than the parent compound.In addition,the first-generation tetracycline antibiotics could be removed,suggesting that strain DM13 has the potential for application in treating antibiotic wastewater.展开更多
文摘Due to various reasons, the inspection methods often need to be changed, and the detection reagents often need to be replaced. In this study, a comparative experiment was conducted between the ethanol-based and ether-based determination methods for oil content in imported wool. The determination results obtained from the two methods were treated as abscissa and ordinate respectively,and their linear relationship was analyzed. According to the linear regression analysis, the conversion equation of determination result between the two methods was obtained. In addition, the repeatability admissible error and reproducibility admissible error were established through analyzing the comparative experimental results by scientific software. This study will bring new ideas for further researches in this field, and provide reference for solving the similar problems in actual inspection work.
基金National Natural Science Foundation of China(No.41001334)Fundamental Research Funds for the Central Universities,China(No.2009B00814)+1 种基金the Project of Knowledge Innovation for the 3rd period,the Chinese Academy of Sciences(No.KZCX2-YW-JS304)Project of the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),China
文摘The developed technique of diffusive gradients in thin film( DGT) has been suggested as a promising tool for evaluation of cadmium( Cd) availability in soils,but there exists considerable debate on its suitability. In this study,Cd bioavailability in soils was systemically investigated by comparing this technique with seven traditional methods, including soil solution concentration and six commonly used extraction methods( HAc,EDTA,Na c,NH4Ac,CaCl2 and MgCl2). Two typical plants( wheat and maize) were examined for Cd uptake. Maize was more sensitive to increasing exposure to Cd in soils than wheat when the added amounts of Cd ranged from 3. 5 to 5. 0 mg · kg-1,accompanied by the significant decreases of shoot and root biomasses. Cd concentrations in shoots and roots of two plants increased continuously with increasing levels of Cd in soils. Cd uptake was higher in wheat than that in maize. The bioavailable concentrations of Cd measured by all methods increased nearly linearly with increasing addition of Cd in soils. Positive correlations were observed between various bioavailable indicators of Cd in soils and Cd concentrations in two plants. The extractable Cd by six chemical extractants was considerably higher for maize than for wheat,while the DGT-measured and soil solution concentrations of Cd were lower for maize than for wheat,following the same trend as plant Cd uptake. The results imply that DGT measurement can effectively predict the bioavailable levels of Cd in soil solutions and that it is an ideal tool for prediction of Cd bioavailability in soils.
基金supported by the Innovation Foundation of Provincial Education Department of Gansu(2024B-005)the Gansu Province National Science Foundation(22YF7GA182)the Fundamental Research Funds for the Central Universities(No.lzujbky2022-kb01)。
文摘Modal parameters can accurately characterize the structural dynamic properties and assess the physical state of the structure.Therefore,it is particularly significant to identify the structural modal parameters according to the monitoring data information in the structural health monitoring(SHM)system,so as to provide a scientific basis for structural damage identification and dynamic model modification.In view of this,this paper reviews methods for identifying structural modal parameters under environmental excitation and briefly describes how to identify structural damages based on the derived modal parameters.The paper primarily introduces data-driven modal parameter recognition methods(e.g.,time-domain,frequency-domain,and time-frequency-domain methods,etc.),briefly describes damage identification methods based on the variations of modal parameters(e.g.,natural frequency,modal shapes,and curvature modal shapes,etc.)and modal validation methods(e.g.,Stability Diagram and Modal Assurance Criterion,etc.).The current status of the application of artificial intelligence(AI)methods in the direction of modal parameter recognition and damage identification is further discussed.Based on the pre-vious analysis,the main development trends of structural modal parameter recognition and damage identification methods are given to provide scientific references for the optimized design and functional upgrading of SHM systems.
基金Supported by National Natural Science Foundation of China(31360544)
文摘Effects of different enucleation methods on developmental potency of pig handmade clone（HMC）reconstructed embryo was investigated in the paper.We compared enucleation efficiency of blind cut method,first polar body（Pb1）positioning method and demecolcine（DM）assisted enucleation,as well as their effects on development of HMC reconstructed embryos.The results showed that overall enucleation efficiency of Pb1 positioning method was significantly higher than that of blind cut method（P〈0.05）.The protuberance rate and overall enucleation efficiency of 0.4μg/mL DM treated group for 60 min was significantly higher than that of other concentrations and time treatment groups（P〈0.05）.For effects on development of HMC reconstructed embryos,there was no significant difference between DM-assisted enucleation and Pb1 positioning method.In conclusion,appropriate addition of DM could enhance enucleation efficiency of HMC,which had no significant influence on developmental potency of reconstructed embryos.
文摘First, the high-rise building structure design process is divided into three relevant steps, that is, scheme generation and creation, performance evaluation, and scheme optimization. Then with the application of relational database, the case database of high-rise structures is constructed, the structure form-selection designing methods such as the smart algorithm based on CBR, DM, FINS, NN and GA is presented, and the original forms system of this method and its general structure are given. CBR and DM are used to generate scheme candidates; FINS and NN to evaluate and optimize the scheme performance; GA to create new structure forms. Finally, the application cases are presented, whose results fit in with the real project. It proves by combining and using the expert intelligence, algorithm intelligence and machine intelligence that this method makes good use of not only the engineering project knowledge and expertise but also much deeper knowledge contained in various engineering cases. In other words, it is because the form selection has a strong background support of vast real cases that its results prove more reliable and more acceptable. So the introduction of this method provides an effective approach to improving the quality, efficiency, automatic and smart level of high-rise structures form selection design.
文摘目的:研究低氧状态下自噬对2型糖尿病(Diabetes mellitus type 2, T2DM)合并股骨骨折大鼠模型不同时间点的骨折愈合程度及低氧诱导因子-1α (HIF-1α)、碱性磷酸酶(alkaline phosphatase, ALP)、Runt-相关转录因子-2 (Runx2)、微管相关蛋白1A/1B-轻链3 (LC3II/I)表达情况。方法:大鼠随机分为高糖高脂饲养前(Control)组、高糖高脂饲养8周后(HFD)组及T2DM模型(T2DM)组,采用高糖髙脂饲料联合链脲佐菌素制备T2DM模型,在T2DM模型基础上制作股骨骨折模型。实验大鼠分为对照组(T2DM合并股骨骨折模型组,Model)及治疗组(氯化钴(CoCl2)治疗的T2DM合并股骨骨折模型组,Treatment),根据检测时间点每组继续分为造模后7 d、28 d、42 d的三个亚组。X线评估股骨愈合情况,WB检测HIF-1α、ALP、Runx2、LC3II/I表达。结果:T2DM合并股骨骨折模型随时间的增长,治疗组在第28 d相比于对照组已有较好的恢复,骨组织中ALP、Runx2表达增加,自噬蛋白LC3II/I的检测结果显示随时间增长自噬水平有所减弱。结论:低氧可以诱导自噬来促进T2DM大鼠骨折愈合。Objective: To investigate fracture healing and hypoxia-inducible factor-1α (HIF-1α), alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), and microtubule associated protein-II/I (LC3II/I) expression at different time points in T2DM rats with femoral fracture under hypoxic conditions. Methods: The rats were randomly divided into high-sugar and high-fat pre-feeding groups, high-sugar and high-fat feeding groups after 8 weeks of feeding and a T2DM model group, T2DM was prepared with high-glucose and high-fat diet combined with streptavidin. Femoral fractures were modeled on the basis of T2DM. Experimental rats were divided into a control group (T2DM with femoral fracture model group, Model) and a treatment group (CoCl2 treatment group, Treatment) and continued to be divided into three subgroups on days 7, 28, and 42 post-molecularization for each group at the time of measurement. Femoral healing was assessed by X-ray. HIF-1α, ALP, Runx2, and LC3II/I expression was measured by Western blotting. Result: In the T2DM combined femoral fracture model, as time progresses, the treatment group shows better recovery compared to the control group at day 28. The expression of ALP and Runx2 in bone tissue increases, and the detection results of autophagy protein LC3II/I indicate that autophagy levels weaken over time. Conclusion: Hypoxia can induce autophagy to promote fracture healing in T2DM rats.
基金funding this work through General Research Project under Grant No.(R.G.P.2/48/43).
文摘According to the World Health Organization(WHO),cancer is the leading cause of death for children in low and middle-income countries.Around 400,000 kids get diagnosed with this illness each year,and their survival rate depends on the country in which they live.In this article,we present a Pythagorean fuzzy model that may help doctors identify the most likely type of cancer in children at an early stage by taking into account the symptoms of different types of cancer.The Pythagorean fuzzy decision-making techniques that we utilize are Pythagorean Fuzzy TOPSIS,Pythagorean Fuzzy Entropy(PF-Entropy),and Pythagorean Fuzzy PowerWeighted Geometric(PFPWG).Ourmodel is fed with nineteen symptoms and it diagnoses the risk of eight types of cancers in children.We develop an algorithm for each method and calculate its complexity.Additionally,we consider an example to make a clear understanding of our model.We also compare the final results of various tests that prove the authenticity of this study.
文摘光伏发电系统的发电效率与最大功率点的捕捉与跟踪技术密切相关。当辐照度、温度及其他外部环境变化时,光伏电池的开路电压、短路电流和最大功率点电压及电流也随之变化。为提高光伏发电效率,寻求光伏电池的最优工作状态,大多数的最大功率点跟踪(Maximum power point tracking,MPPT)主要作用于DC-DC变换器的输入端,该方式在MPPT时会受到变换器损耗的影响,降低最大功率点寻优精度。基于Boost电路,在DC-DC变换器的输出端搭建MPPT系统,通过调节输出电压参数来实现对效果变化的控制,且在MPPT系统引入了导纳微分法(Admittance differentiation method,ADM),实现对MPPT控制器的重构,实施中搭建不同的仿真模型,再借助仿真模型达到对ADM的修正与优化,综合论证该方法具有更优的指标和更好的效果。
基金supported by the Natural Science Basic Research Program of Shaanxi Province(No.2023-JC-JQ-36).
文摘Minocycline has been widely used in clinical treatment and its residues were considered to have environmental safety risks due to complex chemical structure.Therefore,it is necessary to find an efficient and environmentally friendly method to remove minocycline from the environment.This study screened and isolated a minocycline degrading strain DM13 from the activated sludge for municipal sewage pipeline,and optimized the biodegradation of minocycline by DM13 under various environmental conditions.The maximum biodegradation efficiency of 50 mg/L minocycline reached 93%at 72 h with the temperature of 30℃,the initial pH of 7.0,and the inoculation rate of 3%.Two potential biotransformation pathways were proposed,including deamination,demethylation,and decarbonylation.The acute toxicity assessment showed that the biotransformation products of minocycline had lower toxicity than the parent compound.In addition,the first-generation tetracycline antibiotics could be removed,suggesting that strain DM13 has the potential for application in treating antibiotic wastewater.
