Objective To investigate the role of iron death in paraquat(PQ)-induced alveolar epithelial mesangialization(EMT).Methods In August 2023,the appropriate PQ staining concentration as well as the intervention concentrat...Objective To investigate the role of iron death in paraquat(PQ)-induced alveolar epithelial mesangialization(EMT).Methods In August 2023,the appropriate PQ staining concentration as well as the intervention concentration of lipoinhibitor-1(Lip-1)were screened by CCK8 method.The RLE-6TN cells were divided into three groups,which were control group,PQ group and iron death inhibition group,200μmol/L PQ solution was given to the PQ group,and PQ 200μmol/L and 0.1μmol/L Lip-1 solution was given to the iron death inhibition group,the control group was added the same amount of cell medium.morphological changess and migratory viability of the cells in each group were observed at 12 h,24 h and 48 h after the poisoning,and the contents of ferrous ions(Fe^(2+)),reactive oxygen radicals(ROS),glutathione(GSH),superoxide dismutase(SOD),and malondialdehyde(MDA)were detected in each group;meanwhile,qRT-PCRRand western-blot were used to determine the molecular expression of E-cadherin,α-smooth muscle actin(α-SMA),and Collagen I in the cells in each group.The difference between group was compared by ANOVA,and the further pairwise comparison was conducted by Bonferroni method.Results Cell viability was detected using CCK8,and the results showed that the cell survival rate of RLE-6TN cells treated with 200μmol/L PQ+0.1μmol/L Lip-1 solution was 56.6%.The migration activity of RLE-6TN cells in the iron death inhibition group was weaker than that in the PQ group after 24 and 48 hours of exposure,and the degree of EMT changes in the cells was reduced compared to the PQ group.After 12,24,and 48 hours of exposure,the Fe^(2+)concentration,ROS fluorescence intensity,and MDA content in the iron death inhibition group decreased compared to the corresponding time points in the PQ group(P<0.05/3),while compared with PQ group,the GSH concentration and SOD concentration increased compared to the corresponding time points in the PQ group(P<0.05/3).The results showed that compared with the control group,the expression levels of E-cadherin mRNA and protein in PQ group and iron death inhibition group were both decreased(P<0.05/3),while compared with PQ group,the expression levels of E-cadherin mRNA and protein in iron death inhibition group were increased(P<0.05/3);Compared with the control group,the expression levels ofα-SMA,Collagen I mRNA and protein in PQ group and iron death inhibition group cells increased(P<0.05/3),while compared with PQ group,the expression levels ofα-SMA,Collagen I mRNA and protein in iron death inhibition group cells decreased(P<0.05/3).Conclusion Ferroptosis is involved in the EMT process of alveolar epithelial cells induced by PQ.Inhibiting ferroptosis can reduce cellular oxidative damage and alleviate the degree of cellular EMT.展开更多
ObjectiveToinvestigatethe interactionof workplace noise,body mass index(BMI)and systemic inflammatory response on hypertension.Methods In January 2019,1124 male workers from an automobile factory in Wuhan were selecte...ObjectiveToinvestigatethe interactionof workplace noise,body mass index(BMI)and systemic inflammatory response on hypertension.Methods In January 2019,1124 male workers from an automobile factory in Wuhan were selected by cluster random sampling method.The study population was divided into normal body weight group(BMI<24 kg/m^(2))and overweight group(BMI≥24 kg/m^(2))according to BMI,and were followed up for 3 years.The occupational health examination of the workers was carried out every year,blood routine and blood biochemical indexes were collected,and the information of the workers'age,BMI,type of work and age of exposure to injury were collected through field questionnaires.The noise intensity of the workplace of theenterpriseewas measured for 3 consecutive years,and the equivalent sound level was calculated according to the working time.Normal and skewness measurements were described by Mean±SD and[M(Q_(1),Q_(3))],respectively.t-test or Wilcoxon rank sumtestwasusedfor inter-group comparisons.Categorical variables were expressed by frequency and percentage(%),and Chi-square test was used for intergroup comparisons.Mixed effects models and generalized estimationequations were fitted toanalyzethe relationshipbetween occupational noise,systemic inflammation,and their interactionterms with blood pressure and hypertensionrisk.Results Univariate analysis revealed that the white blood cell counts,neutrophil counts,lymphocyte counts and eosinophil counts were positively associated with systolic blood pressure and diastolic blood pressure in overweight group(n=467),respectively(P<0.05).The participants with white blood cell counts≥5.94×10^(9)/L,neutrophil counts≥3.31×10^(9)/L,lymphocyte counts≥2.03×10^(9)/L,and eosinophil counts≥0.12×10^(9)/L had increased risk of hypertension by 52%(RR=1.52,95%CI:1.11-2.05),37%(RR=1.37,95%CI:1.01-1.83),58%(RR=1.58,95%CI:1.18-2.09),and 52%(RR=1.52,95%CI:1.12-2.06),respectively(all P<0.05).Multivariate analysis found a positive association between BMI and the risk of hypertension in the overweight group(P<0.05),the participants with lymphocyte counts≥2.03×10^(9)/L and eosinophil counts≥0.12×10^(9)/L had increased risk of hypertension by 39%(RR=1.39,95%CI:1.01-1.89)and 46%(RR=1.46,95%CI:1.06-1.98),respectively(all P<0.05).There was no correlation between BMI and the risk of hypertension in normal weight group(P>0.05).The multiplicative interaction of occupational noise(≥80 dB)with white blood cell counts(≥5.94×10^(9)/L)and lymphocyte counts(≥2.03×10^(9)/L)increased the risk of hypertension by 100%(RR=2.00,95%CI:1.06-3.55)and 89%(RR=1.89,95%CI:1.01-3.32)(all P<0.05)in overweight group,respectively.Additionally,a significant additive interaction was observed between noise exposure(≥80 dB)and basophil counts(≥0.03×10^(9)/L)on hypertension risk in the overweight group(RERI=0.49,95%CI:0.02-0.96,P<0.05).Conclusion The combined exposure of occupational noise and systemic inflammation may increase the risk of hypertension in overweight workers in automobile manufacturing plants,and the two have additive and multiplicative interactions.展开更多
This article deals with the methods of finding partial discharge(PD)location in power transformers using ultra high frequency(UHF)measurements.The UHF technique utilises two methods to find the PD location,that is,the...This article deals with the methods of finding partial discharge(PD)location in power transformers using ultra high frequency(UHF)measurements.The UHF technique utilises two methods to find the PD location,that is,the shortest path method and hyperbolic method.The shortest path method works based on the comparison of the measured data and the ones in the database.In the hyperbolic method,a hyperbolic equation is obtained between each two element subset of sensors.The coordinate that best fits all equations is known as the PD location,and can be obtained in three different ways,that is,iterative algorithms,the Fang method and Chan method.The convergence of iterative algorithms is limited by poor initial estimate,overshoot,mitigation of non-convergence etc.The Fang and Chan methods are two closed-form solutions that are used in the communication system to find the radiation source location.This article explains how to use these two methods to obtain the PD coordinate inside the power transformer.These two methods can find exactly the coordinate that best fits all hyperbolic equations.At the end of this article,several tests are carried out through CST software and the PD locations is estimated by all presented methods.The simulation results show how the Fang and Chan methods can overcome the limitations of the iterative method.展开更多
The coupled nonlocal nonlinear Schrödinger equations with variable coefficients are researched using the nonstandard Hirota bilinear method.The two-soliton and double-hump one-soliton solutions for the equations ...The coupled nonlocal nonlinear Schrödinger equations with variable coefficients are researched using the nonstandard Hirota bilinear method.The two-soliton and double-hump one-soliton solutions for the equations are first obtained.By assigning different functions to the variable coefficients,we obtain V-shaped,Y-shaped,wave-type,exponential solitons,and so on.Next,we reveal the influence of the real and imaginary parts of the wave numbers on the double-hump structure based on the soliton solutions.Finally,by setting different wave numbers,we can change the distance and transmission direction of the solitons to analyze their dynamic behavior during collisions.This study establishes a theoretical framework for controlling the dynamics of optical fiber in nonlocal nonlinear systems.展开更多
The palaeogeographic map is a graphic representation of physical geographical character- istics in geological history periods and human history periods. It is the most important result of palaeogeographic study. The a...The palaeogeographic map is a graphic representation of physical geographical character- istics in geological history periods and human history periods. It is the most important result of palaeogeographic study. The author, as the Editor-in-Chief oflournal of Palaeogeography, Chinese Edition and English Edition, aimed at the problems of the articles submitted to and published in the]ournal of Palaeogeography in recent years and the relevant papers and books of others, and integrated with his practice of palaeogeographic study and mapping, wrote this paper. The content mainly includes the data of palaeogeographic mapping, the prob- lems of palaeogeographic mapping method, the ＂Single factor analysis and multifactor comprehensive mapping method ---- Methodology of quantitative lithofacies palae- ogeography＇, i.e., the ＂4 steps mapping method＂, the nomenclature of each palaeogeographic unit in palaeogeographic map, the explanation of each palaeogeographic unit in palae- ogeographic map, the explanation of significance of palaeogeographic map and palae- ogeographic article, the evaluative standards of palaeogeographic map and palaeogeographic article, and the self-evaluation. Criticisms and corrections are welcome. 2016 China University of Petroleum {Beijing）. Production and hosting by Elsevier B.V. on behalf of China University of Petroleum （Beijing）. This is an open access article under展开更多
文摘Objective To investigate the role of iron death in paraquat(PQ)-induced alveolar epithelial mesangialization(EMT).Methods In August 2023,the appropriate PQ staining concentration as well as the intervention concentration of lipoinhibitor-1(Lip-1)were screened by CCK8 method.The RLE-6TN cells were divided into three groups,which were control group,PQ group and iron death inhibition group,200μmol/L PQ solution was given to the PQ group,and PQ 200μmol/L and 0.1μmol/L Lip-1 solution was given to the iron death inhibition group,the control group was added the same amount of cell medium.morphological changess and migratory viability of the cells in each group were observed at 12 h,24 h and 48 h after the poisoning,and the contents of ferrous ions(Fe^(2+)),reactive oxygen radicals(ROS),glutathione(GSH),superoxide dismutase(SOD),and malondialdehyde(MDA)were detected in each group;meanwhile,qRT-PCRRand western-blot were used to determine the molecular expression of E-cadherin,α-smooth muscle actin(α-SMA),and Collagen I in the cells in each group.The difference between group was compared by ANOVA,and the further pairwise comparison was conducted by Bonferroni method.Results Cell viability was detected using CCK8,and the results showed that the cell survival rate of RLE-6TN cells treated with 200μmol/L PQ+0.1μmol/L Lip-1 solution was 56.6%.The migration activity of RLE-6TN cells in the iron death inhibition group was weaker than that in the PQ group after 24 and 48 hours of exposure,and the degree of EMT changes in the cells was reduced compared to the PQ group.After 12,24,and 48 hours of exposure,the Fe^(2+)concentration,ROS fluorescence intensity,and MDA content in the iron death inhibition group decreased compared to the corresponding time points in the PQ group(P<0.05/3),while compared with PQ group,the GSH concentration and SOD concentration increased compared to the corresponding time points in the PQ group(P<0.05/3).The results showed that compared with the control group,the expression levels of E-cadherin mRNA and protein in PQ group and iron death inhibition group were both decreased(P<0.05/3),while compared with PQ group,the expression levels of E-cadherin mRNA and protein in iron death inhibition group were increased(P<0.05/3);Compared with the control group,the expression levels ofα-SMA,Collagen I mRNA and protein in PQ group and iron death inhibition group cells increased(P<0.05/3),while compared with PQ group,the expression levels ofα-SMA,Collagen I mRNA and protein in iron death inhibition group cells decreased(P<0.05/3).Conclusion Ferroptosis is involved in the EMT process of alveolar epithelial cells induced by PQ.Inhibiting ferroptosis can reduce cellular oxidative damage and alleviate the degree of cellular EMT.
文摘ObjectiveToinvestigatethe interactionof workplace noise,body mass index(BMI)and systemic inflammatory response on hypertension.Methods In January 2019,1124 male workers from an automobile factory in Wuhan were selected by cluster random sampling method.The study population was divided into normal body weight group(BMI<24 kg/m^(2))and overweight group(BMI≥24 kg/m^(2))according to BMI,and were followed up for 3 years.The occupational health examination of the workers was carried out every year,blood routine and blood biochemical indexes were collected,and the information of the workers'age,BMI,type of work and age of exposure to injury were collected through field questionnaires.The noise intensity of the workplace of theenterpriseewas measured for 3 consecutive years,and the equivalent sound level was calculated according to the working time.Normal and skewness measurements were described by Mean±SD and[M(Q_(1),Q_(3))],respectively.t-test or Wilcoxon rank sumtestwasusedfor inter-group comparisons.Categorical variables were expressed by frequency and percentage(%),and Chi-square test was used for intergroup comparisons.Mixed effects models and generalized estimationequations were fitted toanalyzethe relationshipbetween occupational noise,systemic inflammation,and their interactionterms with blood pressure and hypertensionrisk.Results Univariate analysis revealed that the white blood cell counts,neutrophil counts,lymphocyte counts and eosinophil counts were positively associated with systolic blood pressure and diastolic blood pressure in overweight group(n=467),respectively(P<0.05).The participants with white blood cell counts≥5.94×10^(9)/L,neutrophil counts≥3.31×10^(9)/L,lymphocyte counts≥2.03×10^(9)/L,and eosinophil counts≥0.12×10^(9)/L had increased risk of hypertension by 52%(RR=1.52,95%CI:1.11-2.05),37%(RR=1.37,95%CI:1.01-1.83),58%(RR=1.58,95%CI:1.18-2.09),and 52%(RR=1.52,95%CI:1.12-2.06),respectively(all P<0.05).Multivariate analysis found a positive association between BMI and the risk of hypertension in the overweight group(P<0.05),the participants with lymphocyte counts≥2.03×10^(9)/L and eosinophil counts≥0.12×10^(9)/L had increased risk of hypertension by 39%(RR=1.39,95%CI:1.01-1.89)and 46%(RR=1.46,95%CI:1.06-1.98),respectively(all P<0.05).There was no correlation between BMI and the risk of hypertension in normal weight group(P>0.05).The multiplicative interaction of occupational noise(≥80 dB)with white blood cell counts(≥5.94×10^(9)/L)and lymphocyte counts(≥2.03×10^(9)/L)increased the risk of hypertension by 100%(RR=2.00,95%CI:1.06-3.55)and 89%(RR=1.89,95%CI:1.01-3.32)(all P<0.05)in overweight group,respectively.Additionally,a significant additive interaction was observed between noise exposure(≥80 dB)and basophil counts(≥0.03×10^(9)/L)on hypertension risk in the overweight group(RERI=0.49,95%CI:0.02-0.96,P<0.05).Conclusion The combined exposure of occupational noise and systemic inflammation may increase the risk of hypertension in overweight workers in automobile manufacturing plants,and the two have additive and multiplicative interactions.
文摘This article deals with the methods of finding partial discharge(PD)location in power transformers using ultra high frequency(UHF)measurements.The UHF technique utilises two methods to find the PD location,that is,the shortest path method and hyperbolic method.The shortest path method works based on the comparison of the measured data and the ones in the database.In the hyperbolic method,a hyperbolic equation is obtained between each two element subset of sensors.The coordinate that best fits all equations is known as the PD location,and can be obtained in three different ways,that is,iterative algorithms,the Fang method and Chan method.The convergence of iterative algorithms is limited by poor initial estimate,overshoot,mitigation of non-convergence etc.The Fang and Chan methods are two closed-form solutions that are used in the communication system to find the radiation source location.This article explains how to use these two methods to obtain the PD coordinate inside the power transformer.These two methods can find exactly the coordinate that best fits all hyperbolic equations.At the end of this article,several tests are carried out through CST software and the PD locations is estimated by all presented methods.The simulation results show how the Fang and Chan methods can overcome the limitations of the iterative method.
基金supported by the National Key R&D Program of China(Grant No.2022YFA1604200)the National Natural Science Foundation of China(Grant No.12261131495)Institute of Systems Science,Beijing Wuzi University(Grant No.BWUISS21).
文摘The coupled nonlocal nonlinear Schrödinger equations with variable coefficients are researched using the nonstandard Hirota bilinear method.The two-soliton and double-hump one-soliton solutions for the equations are first obtained.By assigning different functions to the variable coefficients,we obtain V-shaped,Y-shaped,wave-type,exponential solitons,and so on.Next,we reveal the influence of the real and imaginary parts of the wave numbers on the double-hump structure based on the soliton solutions.Finally,by setting different wave numbers,we can change the distance and transmission direction of the solitons to analyze their dynamic behavior during collisions.This study establishes a theoretical framework for controlling the dynamics of optical fiber in nonlocal nonlinear systems.
文摘The palaeogeographic map is a graphic representation of physical geographical character- istics in geological history periods and human history periods. It is the most important result of palaeogeographic study. The author, as the Editor-in-Chief oflournal of Palaeogeography, Chinese Edition and English Edition, aimed at the problems of the articles submitted to and published in the]ournal of Palaeogeography in recent years and the relevant papers and books of others, and integrated with his practice of palaeogeographic study and mapping, wrote this paper. The content mainly includes the data of palaeogeographic mapping, the prob- lems of palaeogeographic mapping method, the ＂Single factor analysis and multifactor comprehensive mapping method ---- Methodology of quantitative lithofacies palae- ogeography＇, i.e., the ＂4 steps mapping method＂, the nomenclature of each palaeogeographic unit in palaeogeographic map, the explanation of each palaeogeographic unit in palae- ogeographic map, the explanation of significance of palaeogeographic map and palae- ogeographic article, the evaluative standards of palaeogeographic map and palaeogeographic article, and the self-evaluation. Criticisms and corrections are welcome. 2016 China University of Petroleum {Beijing）. Production and hosting by Elsevier B.V. on behalf of China University of Petroleum （Beijing）. This is an open access article under
