Photoacoustic mesoscopy(PAMe) offers high-sensitivity in vivo imaging based on the rich optical contrast in biological tissues,with sub-100-micron resolutions at a few millimeters depth. By benefiting from low ultraso...Photoacoustic mesoscopy(PAMe) offers high-sensitivity in vivo imaging based on the rich optical contrast in biological tissues,with sub-100-micron resolutions at a few millimeters depth. By benefiting from low ultrasonic scattering,this emerging technology has pushed the penetration depth beyond the optical diffuse limit unprecedented for high-resolution optical methods.Here,we review ed the state-of-art implementations of PAMe and their achievements in biological and primary clinical applications. With the high-frequency focused ultrasonic detector,the high-resolution optical visualization can be achieved by utilizing various PAMe systems. These capabilities of PAMe have made it well applicable for understanding the biological mechanisms,exploring the pathological features and analyzing the characteristics of human skin. Future improvements and prospects of PAMe are also mentioned,suggesting its great potential tow ards the corresponding emerging biomedical and clinical applications.展开更多
Mesoscopy refers to imaging methodologies that provide a field of view(FOV)ranging from several millimeters to centimeters while achieving cellular or even subcellular resolution(Figure 1).This technological framework...Mesoscopy refers to imaging methodologies that provide a field of view(FOV)ranging from several millimeters to centimeters while achieving cellular or even subcellular resolution(Figure 1).This technological framework employs specially designed large-scale objective lenses to correct aberrations across extended FOVs,synchronized with light-field acquisition modalities through either scanning point detection or large-format array detection.Conventional microscopes,constrained by the limitations of objective lenses,exhibit a trade-off between the FOV and resolution.To achieve both high resolution and a large FOV,common approaches such as FOV stitching and Fourier ptychography were employed.However,these methods were extremely slow and imposed numerous constraints on samples.In 2016,a mesoscopic objective lens was introduced to address these challenges,achieving a 6 mm FOV and 0.7 mm resolution,thereby increasing the imaging throughput of conventional objective lenses by orders of magnitude.1 In the same year,this technology was recognized as one of the top ten physics breakthroughs worldwide by Physics World.Since then,mesoscopic imaging technology has gradually gained momentum and has been applied in various fields.展开更多
Whole-body optical imaging of post-embryonic stage model organisms is a challenging and long sought-after goal.It requires a combination of high-resolution performance and high-penetration depth.Optoacoustic(photoacou...Whole-body optical imaging of post-embryonic stage model organisms is a challenging and long sought-after goal.It requires a combination of high-resolution performance and high-penetration depth.Optoacoustic(photoacoustic)mesoscopy holds great promise,as it penetrates deeper than optical and optoacoustic microscopy while providing high-spatial resolution.However,optoacoustic mesoscopic techniques only offer partial visibility of oriented structures,such as blood vessels,due to a limited angular detection aperture or the use of ultrasound frequencies that yield insufficient resolution.We introduce 3601 multi orientation(multi-projection)raster scan optoacoustic mesoscopy(MORSOM)based on detecting an ultra-wide frequency bandwidth(up to 160 MHz)and weighted deconvolution to synthetically enlarge the angular aperture.We report unprecedented isotropic inplane resolution at the 9–17μm range and improved signal to noise ratio in phantoms and opaque 21-day-old Zebrafish.We find that MORSOM performance defines a new operational specification for optoacoustic mesoscopy of adult organisms,with possible applications in the developmental biology of adulthood and aging.展开更多
基金the National Natural Science Foundation of China (Grant Nos.81401453,81371602,61475115,61475116,61575140,81571723,and 81671728)the Tianjin Municipal Government of China (Grant Nos.14JCQNJC14400,15JCZDJC31800,15JCQNJC14500,and 16JCZDJC31200)
文摘Photoacoustic mesoscopy(PAMe) offers high-sensitivity in vivo imaging based on the rich optical contrast in biological tissues,with sub-100-micron resolutions at a few millimeters depth. By benefiting from low ultrasonic scattering,this emerging technology has pushed the penetration depth beyond the optical diffuse limit unprecedented for high-resolution optical methods.Here,we review ed the state-of-art implementations of PAMe and their achievements in biological and primary clinical applications. With the high-frequency focused ultrasonic detector,the high-resolution optical visualization can be achieved by utilizing various PAMe systems. These capabilities of PAMe have made it well applicable for understanding the biological mechanisms,exploring the pathological features and analyzing the characteristics of human skin. Future improvements and prospects of PAMe are also mentioned,suggesting its great potential tow ards the corresponding emerging biomedical and clinical applications.
基金supported by the Chinese Academy of Sciences Project for Young Scientists in Basic Research(YSBR067)the Natural Science Foundation of Jiangsu Province(BK20240024)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(Y2023087)。
文摘Mesoscopy refers to imaging methodologies that provide a field of view(FOV)ranging from several millimeters to centimeters while achieving cellular or even subcellular resolution(Figure 1).This technological framework employs specially designed large-scale objective lenses to correct aberrations across extended FOVs,synchronized with light-field acquisition modalities through either scanning point detection or large-format array detection.Conventional microscopes,constrained by the limitations of objective lenses,exhibit a trade-off between the FOV and resolution.To achieve both high resolution and a large FOV,common approaches such as FOV stitching and Fourier ptychography were employed.However,these methods were extremely slow and imposed numerous constraints on samples.In 2016,a mesoscopic objective lens was introduced to address these challenges,achieving a 6 mm FOV and 0.7 mm resolution,thereby increasing the imaging throughput of conventional objective lenses by orders of magnitude.1 In the same year,this technology was recognized as one of the top ten physics breakthroughs worldwide by Physics World.Since then,mesoscopic imaging technology has gradually gained momentum and has been applied in various fields.
基金sponsored by the Federal Ministry of Education and Research,Photonic Science Germany,Tech2See-13N12624.
文摘Whole-body optical imaging of post-embryonic stage model organisms is a challenging and long sought-after goal.It requires a combination of high-resolution performance and high-penetration depth.Optoacoustic(photoacoustic)mesoscopy holds great promise,as it penetrates deeper than optical and optoacoustic microscopy while providing high-spatial resolution.However,optoacoustic mesoscopic techniques only offer partial visibility of oriented structures,such as blood vessels,due to a limited angular detection aperture or the use of ultrasound frequencies that yield insufficient resolution.We introduce 3601 multi orientation(multi-projection)raster scan optoacoustic mesoscopy(MORSOM)based on detecting an ultra-wide frequency bandwidth(up to 160 MHz)and weighted deconvolution to synthetically enlarge the angular aperture.We report unprecedented isotropic inplane resolution at the 9–17μm range and improved signal to noise ratio in phantoms and opaque 21-day-old Zebrafish.We find that MORSOM performance defines a new operational specification for optoacoustic mesoscopy of adult organisms,with possible applications in the developmental biology of adulthood and aging.
