Offshore floating photovoltaic systems have tremendous potential to address the energy crisis.As a novel type of float-ing photovoltaic system,membrane structures are increasingly applied due to their advantages of be...Offshore floating photovoltaic systems have tremendous potential to address the energy crisis.As a novel type of float-ing photovoltaic system,membrane structures are increasingly applied due to their advantages of being lightweight and cost-effective.A 1:40 scaled model for laboratory experiments was designed and developed,considering Ocean Sun’s membrane structure.The study aims to investigate the hydrodynamic characteristics of the membrane structure under wave loading by testing its various mo-tion responses and mooring forces at different wave heights and periods.The conclusions indicate that as the wave period decreases within the range of 1.75 to 1.25 s,the heave motion response of the structure decreases,whereas pitch,surge motion response,heave acceleration,and mooring force increase.The amplitudes of various motions and mooring forces of the structure decrease with de-creasing wave height.The hydrodynamic responses under irregular and regular waves follow similar patterns,but the responses and mooring forces induced by irregular waves are more significant.The structure should be designed based on the actual wave height.In addition,the same frequency resonance phenomenon is avoided because the movement period of each degree of freedom is close to the wave period.展开更多
The determination of stress distribution is important for the safe use of membrane structures in practical engineering,which is difficult to be obtained by existing measurement methods and analysis methods.This paper ...The determination of stress distribution is important for the safe use of membrane structures in practical engineering,which is difficult to be obtained by existing measurement methods and analysis methods.This paper proposes a rigid-membrane method to determine the stress distribution of the membrane,which expands the stiffness of the membrane,applies the load of the membrane in equilibrium to the membrane shape of the equilibrium state,and performs nonlinear finite element analysis.The rigid-membrane method inversely acquires the stress distribution of the membrane based only on the shape and load distribution in equilibrium obtained from the numerical simulation of a membrane structure under water loads,and determines the modulus magnitude and mesh size required to rigidize the membrane.The accuracy of the rigid-membrane method is verified by the small differences between the stress distributions obtained from the proposed method and numerical simulations.The equilibrium membrane shape in the actual project can be scanned and reconstructed by the laser scanner system without any pre-processing,and the load is determined by the water level,internal pressure,etc.Based on the actual membrane shape and water load distribution,the rigid-membrane method determines the real stress distribution of the membrane in the test of flat membrane subjected to ponding water,which verifies that the rigid-membrane method is a practical method to determine the stress distribution only by the membrane shape and external load distribution.展开更多
Because of the small stiffness and high flexibility, the tension membrane structure is easy to relax and damage or even destroy under the action of external load, which leads to the occurrence of engineering accidents...Because of the small stiffness and high flexibility, the tension membrane structure is easy to relax and damage or even destroy under the action of external load, which leads to the occurrence of engineering accidents. In this paper, the damped nonlinear vibration of tensioned membrane structure under the coupling action of wind and rain is approximately solved, considering the geometric nonlinearity of membrane surface deformation and the influence of air damping. Applying von Karman’s large deflection theory and D’Alembert’s principle, the governing equations are established for an analytical solution, and the experimental results are compared with the analytical results. The feasibility of this method is verified, which provides some theoretical reference for practical membrane structure engineering design and maintenance.展开更多
By the optimum theory, a new cutting analytical method of the membrane structure is developed. The B-spline curve is applied to make smooth the boundary of the membrane strip. By this method, the cutting accuracy is i...By the optimum theory, a new cutting analytical method of the membrane structure is developed. The B-spline curve is applied to make smooth the boundary of the membrane strip. By this method, the cutting accuracy is improved. Finally, a cutting analysis example of a tension membrane structure is given.展开更多
The interaction between membrane structures and their environment can be either static or dynamic. Static interaction refers to interaction with static air, while dynamic interaction refers to wind and its effects. Th...The interaction between membrane structures and their environment can be either static or dynamic. Static interaction refers to interaction with static air, while dynamic interaction refers to wind and its effects. They can be evaluated by two parameters, added mass and radiation/aerodynamic damping, which are experimentally investigated in this study. The study includes the effects of both the static and dynamic interaction on structural dynamic characteristics, and the relationship between the interaction parameters and the covered area of a membrane structure for the static interaction and the relationship between the interaction parameters and wind direction and speed for the dynamic interaction. Experimental data show that the dynamic interaction is strongly correlated with the structural modes, i.e., the interaction of the symmetric modes is much larger than the anti-synmletric modes; and the influence of the dynamic interaction is significant in wind-induced response analysis and cannot be ignored. In addition, it is concluded that the structural natural frequency is remarkably decreased by this interaction, and the frequency band is significantly broadened.展开更多
A multi-objective optimization method based on Pareto Genetic Algorithm is presented for shape design of membrane structures from a structural view point.Several non-dimensional variables are defined as optimization v...A multi-objective optimization method based on Pareto Genetic Algorithm is presented for shape design of membrane structures from a structural view point.Several non-dimensional variables are defined as optimization variables,which are decision factors of shapes of membrane structures.Three objectives are proposed including maximization of stiffness,maximum uniformity of stress and minimum reaction under external loads.Pareto Multi-objective Genetic Algorithm is introduced to solve the Pareto solutions.Consequently,the dependence of the optimality upon the optimization variables is derived to provide guidelines on how to determine design parameters.Moreover,several examples illustrate the proposed methods and applications.The study shows that the multi-objective optimization method in this paper is feasible and efficient for membrane structures;the research on Pareto solutions can provide explicit and useful guidelines for shape design of membrane structures.展开更多
In routine design of tensioned membrane structures, the membrane is generally modeled using space membrane elements and the cables by space cable elements, with no sliding allowed between the membrane and the cables. ...In routine design of tensioned membrane structures, the membrane is generally modeled using space membrane elements and the cables by space cable elements, with no sliding allowed between the membrane and the cables. On the other hand, large deflections are expected and sliding between the membrane and the cables is inevitable. In the present paper, the general finite element code ABAQUS was employed to investigate the influence of cable sliding on membrane surface on the structural behavior. Three analysis models were devised to fulfill this purpose: (1) The membrane element shares nodes with the cable element; (2) The cable can slide on the membrane surface freely (without friction) and (3) The cable can slide on the membrane surface, but with friction between the cable and the membrane. The sliding problem is modeled using a surface - based contact algorithm. The results from three analysis models are compared, showing that cable sliding has only little influence on the structure shape and on the stress distributions in the membrane. The main influence of cable sliding may be its effect on the dynamic behavior of tensioned membrane structures.展开更多
The Enterovirus 71(EV71)VP4 is co-translationally linked to myristic acid at its amino-terminal glycine residue.However,the role of this myristoylation in the EV71 life cycle remains largely unknown.To investigate thi...The Enterovirus 71(EV71)VP4 is co-translationally linked to myristic acid at its amino-terminal glycine residue.However,the role of this myristoylation in the EV71 life cycle remains largely unknown.To investigate this issue,we developed a myristoylation-deficient virus and reporter(luciferase)pseudovirus with a Gly-to-Ala mutation(G2A)on EV71 VP4.When transfecting the EV71-G2 A genome encoding plasmid in cells,the loss of myristoylation on VP4 did not affect the expression of viral proteins and the virus morphology,however,it did significantly influence viral infectivity.Further,in myristoylation-deficient reporter pseudovirus-infected cells,the luciferase activity and viral genome RNA decreased significantly as compared to that of wild type virus;however,cytopathic effect and viral capsid proteins were not detected in myristoylation-deficient virus-infected cells.Also,although myristoylation-deficient viral RNA and proteins were detected in the second blind passage of infection,they were much fewer in number compared to that of the wild type virus.The replication of genomic RNA and negative-strand viral RNA were both blocked in myristoylation-deficient viruses,suggesting that myristoylation affects viral genome RNA release from capsid to cytoplasm.Besides,loss of myristoylation on VP4 altered the distribution of VP4-green fluorescent protein protein,which disappeared from the membrane structure fraction.Finally,a liposome leakage assay showed that EV71 myristoylation mediates the permeability of the model membrane.Hence,the amino-terminal myristoylation of VP4 is pivotal to EV71 infection and capsidmembrane structure interaction.This study provides novel molecular mechanisms regarding EV71 infection and potential molecular targets for antiviral drug design.展开更多
Conical membrane structures are a typical form of tensile membrane structures. In the past, most studies focused on the static performance, but few on dynamic performance. In this paper, systematic parameter analysis ...Conical membrane structures are a typical form of tensile membrane structures. In the past, most studies focused on the static performance, but few on dynamic performance. In this paper, systematic parameter analysis of wind-induced response of conical membrane structures has been performed with nonlinear random simulation method in a time domain, by considering some parameters, such as span, rise-span ratio, prestress of membrane, and characteristic of the approaching wind flow. Moreover, formulas of the dynamic coefficient and nonlinear adjustment factor are advised, which can be conveniently used in wind-resistant design of conical membrane structures.展开更多
Practical techniques for smooth geodesic patterning of membrane structures were investigated.For the geodesic search,adjustment of the subplane of the extracted elements series was proposed,and various spline approxim...Practical techniques for smooth geodesic patterning of membrane structures were investigated.For the geodesic search,adjustment of the subplane of the extracted elements series was proposed,and various spline approximation methods were used to flatten the strip for the generation of a smooth pattern.This search approach is very simple,and the geodesic line could be easily attained by the proposed method without the need for a difficult computation method.Smooth cutting patterning can also be generated by spline approximation without the noise in discrete nodal information.Additionally,the geodesic cutting pattern saved about 21%of the required area for the catenary model due to the reduction of the curvature of the planar pattern seam line.展开更多
An analytical approach based on the bifurcation theory is presented,in which the wrinkles are treated as the local buckling phenomena of the elastic thin plate with little bending stiffness.The average wrinkling wavel...An analytical approach based on the bifurcation theory is presented,in which the wrinkles are treated as the local buckling phenomena of the elastic thin plate with little bending stiffness.The average wrinkling wavelength was determined by incorporating the stress field and the out-of-plane force equilibrium condition of the wrinkled membrane.The wrinkling amplitude was then obtained by associating the characteristics of wrinkling texture with the obtained wrinkling wavelength.Results reveal that the wrinkled pattern exhibits a noticeable difference when the tension load is changed gradually,and two wrinkling styles are identified.The first style occurs for symmetric and moderately asymmetric loading,and it is characterized by small,radial corner wrinkles;the second style occurs for strongly asymmetric loading and is characterized by a deep,large diagonal wrinkle.The analytical predictions on the wrinkling characteristics and the developed rules are validated against wrinkling experimental observations.展开更多
The purpose of this paper is to demonstrate and investigate the concepts of new deployable boom systems, which consist of the BCON （braid coated bi-convex tape） boom and the SMA-BCON （braid coated bi-shape memory a...The purpose of this paper is to demonstrate and investigate the concepts of new deployable boom systems, which consist of the BCON （braid coated bi-convex tape） boom and the SMA-BCON （braid coated bi-shape memory alloy convex tape） boom. Both booms are developed for the deployable membrane structures such as solar sails, thin membrane solar array panels, deorbit mechanisms for small satellites and reflectors of space solar power satellite, etc. BCON booms can store around polygonal or cylindrical center hub, and the booms can deploy by the stepwise manner by releasing a constraint mechanism which pins the boons into two or three points for the total length. SMA-BCON booms are mainly developed for a square center body systems, and SMA is adapted on the bent po^nts of the booms where stored around each edge of the center hub. Through the deployment experiments of both booms, the stepwise deployment behavior and its tendency are obtained. The design concept of BCON boom and SMA-BCON hnnm i~ demonstrated through this study.展开更多
Loading analysis,as one of the most crucial procedures in the assessment of safety of the membrane structures,is to check the adequacy of structural stiffness and the extent of membrane stress and deformation,thus ens...Loading analysis,as one of the most crucial procedures in the assessment of safety of the membrane structures,is to check the adequacy of structural stiffness and the extent of membrane stress and deformation,thus ensuring the structural stability and avoiding the wrinkling of membrane structures.In this paper,the analysis procedures are presented and the wrinkling of membrane structure,representing a major behavioral performance of the structure,is included with the modified constitutive relationship method.As for the judgment of the membrane wrinkling,the principal stress-strain criteria are incorporated.Thereafter,an integrated algorithm is developed,in which the Newton-Raphson method and updated Lagrange formulation are adopted.With the proposed algorithm,two fabric roof structures are analyzed.Results show that the presented method is feasible and reliable.展开更多
The whole analysis process of pneumatic stressed membrane structure contains nine states and seven analysis processes.The zero-stress state is the corner-stone of analysis and design of pneumatic stressed structure,an...The whole analysis process of pneumatic stressed membrane structure contains nine states and seven analysis processes.The zero-stress state is the corner-stone of analysis and design of pneumatic stressed structure,and has significant impact on the pre-stressed state and load state.According to the logical model of the whole numerical analysis process of pneumatic stressed structure,a numerical analysis method to solve the zero-stress state from the elasticized equilibrium state was firstly proposed,called linear compatibility matrix M-P inverse method.Firstly,the pneumatic membrane stressed structure was transferred into grid structure by using membrane link to simulate membrane surface.Secondly,on the basis of equilibrium matrix theory of pin joint structure and small deformation assumption,compatibility equation of system was established.Thirdly,the unstressed length and elongation of links were calculated from the tension and material parameters of elasticized equilibrium state.Finally,using compatibility matrix M-P inverse,the nodal displacement was calculated by solving compatibility equation,the configuration of zero-stress state could be obtained through reverse superposition,and the stress was released.According to the algorithm,the program was coded with MATLAB.The correctness and efficiency of this method were verified by several numerical examples,and it could be found that one elasticized equilibrium state corresponded to one configuration of the zero-stress state.The work has theoretical significance and practical guidance value for pneumatic membrane structural design.展开更多
Microporous membranes of low-high density polyethylene and their blends were prepared bythermally-induced phase separation of polymer/long-aliphatic chain alcohol (diluent) mixtures.The microstructures of this particu...Microporous membranes of low-high density polyethylene and their blends were prepared bythermally-induced phase separation of polymer/long-aliphatic chain alcohol (diluent) mixtures.The microstructures of this particular membrane, which depends on the diluent properties,polymer concentration and cooling rate, were observed by scanning electron microscopy.'Beehive-type,'leafy-like, and lacy porous structure morphologies can be formed,depending onthe blend composition and phase separation conditions, which were discussed by the polymer anddiluent crystallization processes.展开更多
Focal adhesions play an important role in cell spreading,migration,and overall mechanical integrity.The relationship of cell structural and mechanical properties was investigated in the context of focal adhesion proce...Focal adhesions play an important role in cell spreading,migration,and overall mechanical integrity.The relationship of cell structural and mechanical properties was investigated in the context of focal adhesion processes.Combined atomic force microscopy(AFM) and laser scanning confocal microscopy(LSCM) was utilized to measure single cell mechanics,in correlation with cellular morphology and membrane structures at a nanometer scale.Characteristic stages of focal adhesion were verified via confocal fluorescent studies,which confirmed three representative F-actin assemblies,actin dot,filaments network,and long and aligned fibrous bundles at cytoskeleton.Force-deformation profiles of living cells were measured at the single cell level,and displayed as a function of height deformation,relative height deformation and relative volume deformation.As focal adhesion progresses,single cell compression profiles indicate that both membrane and cytoskeleton stiffen,while spreading increases especially from focal complex to focal adhesion.Correspondingly,AFM imaging reveals morphological geometries of spherical cap,spreading with polygon boundaries,and elongated or polarized spreading.Membrane features are dominated by protrusions of 41-207 nm tall,short rods with 1-6 μm in length and 10.2-80.0 nm in height,and long fibrous features of 31-246 nm tall,respectively.The protrusion is attributed to local membrane folding,and the rod and fibrous features are consistent with bilayer decorating over the F-actin assemblies.Taken collectively,the reassembly of F-actin during focal adhesion formation is most likely responsible for the changes in cellular mechanics,spreading morphology,and membrane structural features.展开更多
Water management has been proven significant for enhancing both performance and durability of anion exchange membrane fuel cell. Besides searching new material, decreasing membrane thickness and modifying operation pa...Water management has been proven significant for enhancing both performance and durability of anion exchange membrane fuel cell. Besides searching new material, decreasing membrane thickness and modifying operation parameters, a simple and universal method of altering membrane structure is proposed in this work. Composite membranes made by unique processing method that includes both casting and electrospinning processes were compared with traditional casting membrane, all with the same thickness of 40 μm. Comparing to traditional casting membrane, the composite membrane put at proper position shows a higher water permeability, thus even more than 50% enhancement of the peak performance.展开更多
To know the effects of irradiation on the konjac glucomannan （KGM） molecular chain membrane, KGM membrane solution was treated with the irradiation dose of 0-20 kGy in this study, and the structure and properties of...To know the effects of irradiation on the konjac glucomannan （KGM） molecular chain membrane, KGM membrane solution was treated with the irradiation dose of 0-20 kGy in this study, and the structure and properties of KGM membrane were analyzed with Infrared spectrum, Raman spectrum, X-ray, SEM scanning and so on. The results revealed that the effects of different irradiation doses on the KGM molecular chain structure were different. Higher irradiation dose （20 kGy） resulted in partial damage against KGM membrane crystal structure, and there was no obvious change for the amorphous structure; with membrane property test, the tensile strength of KGM membrane gradually increased with the increase of irradiation dose and its elongation at break reduced, but these changes were not significant, WVP value reduced; with SEM, the membrane surface treated with irradiation was smoother even than the membrane without treatment. In addition, when increasing the irradiation dose, membrane surface became more even, and arrangement was more orderly and compact. KGM membrane nrooerties, and it is an ideal Irradiation modification could effectively improve the modification method.展开更多
In this article, the mathematical model of the coupling of the three-dimensional fluid flow and the large deformation of membrane structure is established. The fluid-structure coupling interaction is simulated using t...In this article, the mathematical model of the coupling of the three-dimensional fluid flow and the large deformation of membrane structure is established. The fluid-structure coupling interaction is simulated using the computational codes developed by the authors. By analyzing the interactions of membrane and flow field, the aeroelasticity of the airship is detailed. All the results are adopted in the focused study of the stratosphere airship in trimmed state.展开更多
A second shape finding method was developed to improve the nonlinear finite element based shape finding method. The curved shape is obtained by raising the control points above the projection plane. The convergence wa...A second shape finding method was developed to improve the nonlinear finite element based shape finding method. The curved shape is obtained by raising the control points above the projection plane. The convergence was improved using pseudo material properties to get a preliminary shape, and then using the real properties to get the final shape. A large number of examples were analyzed to verify the validity and practicality of this method. The results show that the final curved surface after the second shape finding process is always quite similar to the first one. Moreover, the curved surface obtained after the second shape finding process is accurate and will be realized in real materials.展开更多
基金supported by the National Natural Science Foundation of China(No.52271287).
文摘Offshore floating photovoltaic systems have tremendous potential to address the energy crisis.As a novel type of float-ing photovoltaic system,membrane structures are increasingly applied due to their advantages of being lightweight and cost-effective.A 1:40 scaled model for laboratory experiments was designed and developed,considering Ocean Sun’s membrane structure.The study aims to investigate the hydrodynamic characteristics of the membrane structure under wave loading by testing its various mo-tion responses and mooring forces at different wave heights and periods.The conclusions indicate that as the wave period decreases within the range of 1.75 to 1.25 s,the heave motion response of the structure decreases,whereas pitch,surge motion response,heave acceleration,and mooring force increase.The amplitudes of various motions and mooring forces of the structure decrease with de-creasing wave height.The hydrodynamic responses under irregular and regular waves follow similar patterns,but the responses and mooring forces induced by irregular waves are more significant.The structure should be designed based on the actual wave height.In addition,the same frequency resonance phenomenon is avoided because the movement period of each degree of freedom is close to the wave period.
基金the National Natural Science Foundation of China(No.51978395)。
文摘The determination of stress distribution is important for the safe use of membrane structures in practical engineering,which is difficult to be obtained by existing measurement methods and analysis methods.This paper proposes a rigid-membrane method to determine the stress distribution of the membrane,which expands the stiffness of the membrane,applies the load of the membrane in equilibrium to the membrane shape of the equilibrium state,and performs nonlinear finite element analysis.The rigid-membrane method inversely acquires the stress distribution of the membrane based only on the shape and load distribution in equilibrium obtained from the numerical simulation of a membrane structure under water loads,and determines the modulus magnitude and mesh size required to rigidize the membrane.The accuracy of the rigid-membrane method is verified by the small differences between the stress distributions obtained from the proposed method and numerical simulations.The equilibrium membrane shape in the actual project can be scanned and reconstructed by the laser scanner system without any pre-processing,and the load is determined by the water level,internal pressure,etc.Based on the actual membrane shape and water load distribution,the rigid-membrane method determines the real stress distribution of the membrane in the test of flat membrane subjected to ponding water,which verifies that the rigid-membrane method is a practical method to determine the stress distribution only by the membrane shape and external load distribution.
文摘Because of the small stiffness and high flexibility, the tension membrane structure is easy to relax and damage or even destroy under the action of external load, which leads to the occurrence of engineering accidents. In this paper, the damped nonlinear vibration of tensioned membrane structure under the coupling action of wind and rain is approximately solved, considering the geometric nonlinearity of membrane surface deformation and the influence of air damping. Applying von Karman’s large deflection theory and D’Alembert’s principle, the governing equations are established for an analytical solution, and the experimental results are compared with the analytical results. The feasibility of this method is verified, which provides some theoretical reference for practical membrane structure engineering design and maintenance.
文摘By the optimum theory, a new cutting analytical method of the membrane structure is developed. The B-spline curve is applied to make smooth the boundary of the membrane strip. By this method, the cutting accuracy is improved. Finally, a cutting analysis example of a tension membrane structure is given.
基金National Natural Science Foundation of China Under Grant No. 50725826, 90815021
文摘The interaction between membrane structures and their environment can be either static or dynamic. Static interaction refers to interaction with static air, while dynamic interaction refers to wind and its effects. They can be evaluated by two parameters, added mass and radiation/aerodynamic damping, which are experimentally investigated in this study. The study includes the effects of both the static and dynamic interaction on structural dynamic characteristics, and the relationship between the interaction parameters and the covered area of a membrane structure for the static interaction and the relationship between the interaction parameters and wind direction and speed for the dynamic interaction. Experimental data show that the dynamic interaction is strongly correlated with the structural modes, i.e., the interaction of the symmetric modes is much larger than the anti-synmletric modes; and the influence of the dynamic interaction is significant in wind-induced response analysis and cannot be ignored. In addition, it is concluded that the structural natural frequency is remarkably decreased by this interaction, and the frequency band is significantly broadened.
基金Sponsored by the National Natural Science Foundation of China(Grant No.50608022)
文摘A multi-objective optimization method based on Pareto Genetic Algorithm is presented for shape design of membrane structures from a structural view point.Several non-dimensional variables are defined as optimization variables,which are decision factors of shapes of membrane structures.Three objectives are proposed including maximization of stiffness,maximum uniformity of stress and minimum reaction under external loads.Pareto Multi-objective Genetic Algorithm is introduced to solve the Pareto solutions.Consequently,the dependence of the optimality upon the optimization variables is derived to provide guidelines on how to determine design parameters.Moreover,several examples illustrate the proposed methods and applications.The study shows that the multi-objective optimization method in this paper is feasible and efficient for membrane structures;the research on Pareto solutions can provide explicit and useful guidelines for shape design of membrane structures.
文摘In routine design of tensioned membrane structures, the membrane is generally modeled using space membrane elements and the cables by space cable elements, with no sliding allowed between the membrane and the cables. On the other hand, large deflections are expected and sliding between the membrane and the cables is inevitable. In the present paper, the general finite element code ABAQUS was employed to investigate the influence of cable sliding on membrane surface on the structural behavior. Three analysis models were devised to fulfill this purpose: (1) The membrane element shares nodes with the cable element; (2) The cable can slide on the membrane surface freely (without friction) and (3) The cable can slide on the membrane surface, but with friction between the cable and the membrane. The sliding problem is modeled using a surface - based contact algorithm. The results from three analysis models are compared, showing that cable sliding has only little influence on the structure shape and on the stress distributions in the membrane. The main influence of cable sliding may be its effect on the dynamic behavior of tensioned membrane structures.
基金supported by the National Natural Science Foundation of China(Grant No.31770184)
文摘The Enterovirus 71(EV71)VP4 is co-translationally linked to myristic acid at its amino-terminal glycine residue.However,the role of this myristoylation in the EV71 life cycle remains largely unknown.To investigate this issue,we developed a myristoylation-deficient virus and reporter(luciferase)pseudovirus with a Gly-to-Ala mutation(G2A)on EV71 VP4.When transfecting the EV71-G2 A genome encoding plasmid in cells,the loss of myristoylation on VP4 did not affect the expression of viral proteins and the virus morphology,however,it did significantly influence viral infectivity.Further,in myristoylation-deficient reporter pseudovirus-infected cells,the luciferase activity and viral genome RNA decreased significantly as compared to that of wild type virus;however,cytopathic effect and viral capsid proteins were not detected in myristoylation-deficient virus-infected cells.Also,although myristoylation-deficient viral RNA and proteins were detected in the second blind passage of infection,they were much fewer in number compared to that of the wild type virus.The replication of genomic RNA and negative-strand viral RNA were both blocked in myristoylation-deficient viruses,suggesting that myristoylation affects viral genome RNA release from capsid to cytoplasm.Besides,loss of myristoylation on VP4 altered the distribution of VP4-green fluorescent protein protein,which disappeared from the membrane structure fraction.Finally,a liposome leakage assay showed that EV71 myristoylation mediates the permeability of the model membrane.Hence,the amino-terminal myristoylation of VP4 is pivotal to EV71 infection and capsidmembrane structure interaction.This study provides novel molecular mechanisms regarding EV71 infection and potential molecular targets for antiviral drug design.
基金Sponsored by the National Natural Science Foundation of China(Grant No.50338010).
文摘Conical membrane structures are a typical form of tensile membrane structures. In the past, most studies focused on the static performance, but few on dynamic performance. In this paper, systematic parameter analysis of wind-induced response of conical membrane structures has been performed with nonlinear random simulation method in a time domain, by considering some parameters, such as span, rise-span ratio, prestress of membrane, and characteristic of the approaching wind flow. Moreover, formulas of the dynamic coefficient and nonlinear adjustment factor are advised, which can be conveniently used in wind-resistant design of conical membrane structures.
基金Project(12 High-tech Urban C22)supported by High-tech Urban Development Program,Ministry of Land,Transport and Moritime Affairs of Korea
文摘Practical techniques for smooth geodesic patterning of membrane structures were investigated.For the geodesic search,adjustment of the subplane of the extracted elements series was proposed,and various spline approximation methods were used to flatten the strip for the generation of a smooth pattern.This search approach is very simple,and the geodesic line could be easily attained by the proposed method without the need for a difficult computation method.Smooth cutting patterning can also be generated by spline approximation without the noise in discrete nodal information.Additionally,the geodesic cutting pattern saved about 21%of the required area for the catenary model due to the reduction of the curvature of the planar pattern seam line.
基金the Postdoctoral Science Foundation of China(Grant No.20070420163)the Development Program for Outstanding Young Teachers in Harbin Institute of Technology(Grant No.HITQNJS.2008.004)
文摘An analytical approach based on the bifurcation theory is presented,in which the wrinkles are treated as the local buckling phenomena of the elastic thin plate with little bending stiffness.The average wrinkling wavelength was determined by incorporating the stress field and the out-of-plane force equilibrium condition of the wrinkled membrane.The wrinkling amplitude was then obtained by associating the characteristics of wrinkling texture with the obtained wrinkling wavelength.Results reveal that the wrinkled pattern exhibits a noticeable difference when the tension load is changed gradually,and two wrinkling styles are identified.The first style occurs for symmetric and moderately asymmetric loading,and it is characterized by small,radial corner wrinkles;the second style occurs for strongly asymmetric loading and is characterized by a deep,large diagonal wrinkle.The analytical predictions on the wrinkling characteristics and the developed rules are validated against wrinkling experimental observations.
文摘The purpose of this paper is to demonstrate and investigate the concepts of new deployable boom systems, which consist of the BCON （braid coated bi-convex tape） boom and the SMA-BCON （braid coated bi-shape memory alloy convex tape） boom. Both booms are developed for the deployable membrane structures such as solar sails, thin membrane solar array panels, deorbit mechanisms for small satellites and reflectors of space solar power satellite, etc. BCON booms can store around polygonal or cylindrical center hub, and the booms can deploy by the stepwise manner by releasing a constraint mechanism which pins the boons into two or three points for the total length. SMA-BCON booms are mainly developed for a square center body systems, and SMA is adapted on the bent po^nts of the booms where stored around each edge of the center hub. Through the deployment experiments of both booms, the stepwise deployment behavior and its tendency are obtained. The design concept of BCON boom and SMA-BCON hnnm i~ demonstrated through this study.
基金supported by the National Natural Science Foundation of China (Grant Nos. 90815021,50725826)
文摘Loading analysis,as one of the most crucial procedures in the assessment of safety of the membrane structures,is to check the adequacy of structural stiffness and the extent of membrane stress and deformation,thus ensuring the structural stability and avoiding the wrinkling of membrane structures.In this paper,the analysis procedures are presented and the wrinkling of membrane structure,representing a major behavioral performance of the structure,is included with the modified constitutive relationship method.As for the judgment of the membrane wrinkling,the principal stress-strain criteria are incorporated.Thereafter,an integrated algorithm is developed,in which the Newton-Raphson method and updated Lagrange formulation are adopted.With the proposed algorithm,two fabric roof structures are analyzed.Results show that the presented method is feasible and reliable.
基金supported by the National Natural Science Foundation of China (Grant Nos. 50878128, 50808122)
文摘The whole analysis process of pneumatic stressed membrane structure contains nine states and seven analysis processes.The zero-stress state is the corner-stone of analysis and design of pneumatic stressed structure,and has significant impact on the pre-stressed state and load state.According to the logical model of the whole numerical analysis process of pneumatic stressed structure,a numerical analysis method to solve the zero-stress state from the elasticized equilibrium state was firstly proposed,called linear compatibility matrix M-P inverse method.Firstly,the pneumatic membrane stressed structure was transferred into grid structure by using membrane link to simulate membrane surface.Secondly,on the basis of equilibrium matrix theory of pin joint structure and small deformation assumption,compatibility equation of system was established.Thirdly,the unstressed length and elongation of links were calculated from the tension and material parameters of elasticized equilibrium state.Finally,using compatibility matrix M-P inverse,the nodal displacement was calculated by solving compatibility equation,the configuration of zero-stress state could be obtained through reverse superposition,and the stress was released.According to the algorithm,the program was coded with MATLAB.The correctness and efficiency of this method were verified by several numerical examples,and it could be found that one elasticized equilibrium state corresponded to one configuration of the zero-stress state.The work has theoretical significance and practical guidance value for pneumatic membrane structural design.
文摘Microporous membranes of low-high density polyethylene and their blends were prepared bythermally-induced phase separation of polymer/long-aliphatic chain alcohol (diluent) mixtures.The microstructures of this particular membrane, which depends on the diluent properties,polymer concentration and cooling rate, were observed by scanning electron microscopy.'Beehive-type,'leafy-like, and lacy porous structure morphologies can be formed,depending onthe blend composition and phase separation conditions, which were discussed by the polymer anddiluent crystallization processes.
基金initiated by a UCD Alzheimer's Disease Center (ADC) pilotgranta CRCC (Cancer Research Coordination Committee) Research Grantthe support of W. M. Keck Foundation
文摘Focal adhesions play an important role in cell spreading,migration,and overall mechanical integrity.The relationship of cell structural and mechanical properties was investigated in the context of focal adhesion processes.Combined atomic force microscopy(AFM) and laser scanning confocal microscopy(LSCM) was utilized to measure single cell mechanics,in correlation with cellular morphology and membrane structures at a nanometer scale.Characteristic stages of focal adhesion were verified via confocal fluorescent studies,which confirmed three representative F-actin assemblies,actin dot,filaments network,and long and aligned fibrous bundles at cytoskeleton.Force-deformation profiles of living cells were measured at the single cell level,and displayed as a function of height deformation,relative height deformation and relative volume deformation.As focal adhesion progresses,single cell compression profiles indicate that both membrane and cytoskeleton stiffen,while spreading increases especially from focal complex to focal adhesion.Correspondingly,AFM imaging reveals morphological geometries of spherical cap,spreading with polygon boundaries,and elongated or polarized spreading.Membrane features are dominated by protrusions of 41-207 nm tall,short rods with 1-6 μm in length and 10.2-80.0 nm in height,and long fibrous features of 31-246 nm tall,respectively.The protrusion is attributed to local membrane folding,and the rod and fibrous features are consistent with bilayer decorating over the F-actin assemblies.Taken collectively,the reassembly of F-actin during focal adhesion formation is most likely responsible for the changes in cellular mechanics,spreading morphology,and membrane structural features.
基金supported by the National Key Research and Development Program of China (Grant No. 2016YFB0101205)the Natural Science Foundation-Liaoning United Fund (Grant No. U1508202)+1 种基金the Key Laboratory of Fuel Cells&Hybrid Power SourcesChinese Academy of Sciences。
文摘Water management has been proven significant for enhancing both performance and durability of anion exchange membrane fuel cell. Besides searching new material, decreasing membrane thickness and modifying operation parameters, a simple and universal method of altering membrane structure is proposed in this work. Composite membranes made by unique processing method that includes both casting and electrospinning processes were compared with traditional casting membrane, all with the same thickness of 40 μm. Comparing to traditional casting membrane, the composite membrane put at proper position shows a higher water permeability, thus even more than 50% enhancement of the peak performance.
基金supported by the National Natural Science Foundation of China(30871749,30901004 and 31071518)the Natural Science Foundation of Fujian Province(2011J01285)+3 种基金the Key Research Project of Guangdong Province(2010B080701079)the doctoral program of higher education of the specialized research fund for the project funded by the United(20113515110010)the Yangcheng Scholars Technology Project of Guangzhou(10B005D)the Fuzhou Science and Technology Plan Project(2011-N-44)
文摘To know the effects of irradiation on the konjac glucomannan （KGM） molecular chain membrane, KGM membrane solution was treated with the irradiation dose of 0-20 kGy in this study, and the structure and properties of KGM membrane were analyzed with Infrared spectrum, Raman spectrum, X-ray, SEM scanning and so on. The results revealed that the effects of different irradiation doses on the KGM molecular chain structure were different. Higher irradiation dose （20 kGy） resulted in partial damage against KGM membrane crystal structure, and there was no obvious change for the amorphous structure; with membrane property test, the tensile strength of KGM membrane gradually increased with the increase of irradiation dose and its elongation at break reduced, but these changes were not significant, WVP value reduced; with SEM, the membrane surface treated with irradiation was smoother even than the membrane without treatment. In addition, when increasing the irradiation dose, membrane surface became more even, and arrangement was more orderly and compact. KGM membrane nrooerties, and it is an ideal Irradiation modification could effectively improve the modification method.
文摘In this article, the mathematical model of the coupling of the three-dimensional fluid flow and the large deformation of membrane structure is established. The fluid-structure coupling interaction is simulated using the computational codes developed by the authors. By analyzing the interactions of membrane and flow field, the aeroelasticity of the airship is detailed. All the results are adopted in the focused study of the stratosphere airship in trimmed state.
文摘A second shape finding method was developed to improve the nonlinear finite element based shape finding method. The curved shape is obtained by raising the control points above the projection plane. The convergence was improved using pseudo material properties to get a preliminary shape, and then using the real properties to get the final shape. A large number of examples were analyzed to verify the validity and practicality of this method. The results show that the final curved surface after the second shape finding process is always quite similar to the first one. Moreover, the curved surface obtained after the second shape finding process is accurate and will be realized in real materials.
