It is well known that in most cases, a reference is necessary for structural health diagnosis, and it is very difficult to obtain such a reference for a given structure. In this paper, a clan member signal method （C...It is well known that in most cases, a reference is necessary for structural health diagnosis, and it is very difficult to obtain such a reference for a given structure. In this paper, a clan member signal method （CMSM） is proposed for use in structures consisting of groups （or clans） that have the same geometry, i.e., the same cross section and length, and identical boundary conditions. It is expected that signals measured on any undamaged member in a clan after an event could be used as a reference for any other members in the clan. To verify the applicability of the proposed method, a steel truss model is tested and the results show that the CMSM is very effective in detecting local damage in structures composed of identical slender members.展开更多
目的观察腓肠肌钝挫伤白兔的腓肠肌中的无翅型MMTV整合位点家族成员5a(wingless-type MMTV integration site family member 5a,Wnt5a)、糖原合成酶激酶3(glycogen synthase kinase 3,GSK3)/β-联蛋白(β-catenin)的mRNA和蛋白表达,以...目的观察腓肠肌钝挫伤白兔的腓肠肌中的无翅型MMTV整合位点家族成员5a(wingless-type MMTV integration site family member 5a,Wnt5a)、糖原合成酶激酶3(glycogen synthase kinase 3,GSK3)/β-联蛋白(β-catenin)的mRNA和蛋白表达,以及病理染色中呈现的肌肉纤维、脂肪组织的情况,为揭示按揉法治疗骨骼肌损伤的修复机制提供依据。方法选择健康新西兰白兔42只,雌雄各半。采用随机数字表法将其分为空白组、模型3 d组、模型7 d组、模型14 d组、按揉3 d组、按揉7 d组、按揉14 d组,每组6只。各模型组、按揉组在操作后第4、8、15天取材。采用实时定量聚合酶链反应和蛋白质印迹法检测Wnt5a、GSK3、β-catenin的mRNA和蛋白表达情况;采用苏木精-伊红(hematoxylin and eosin,HE)染色和油红O染色观察肌肉组织肌纤维及脂肪组织的情况。结果HE染色显示,模型7 d组发生显著纤维组织增生和炎性细胞浸润;模型14 d组可见部分肌纤维变性坏死、再生伴纤维组织增生及轻微炎性细胞浸润,轻微钙化;各按揉组可见明显肌纤维变性坏死、再生,炎性细胞浸润伴随显著纤维组织增生。油红O染色显示,各模型组可见脂肪细胞沉积,其中模型7 d组最重;各按揉组肌肉纤维及序列未见明显破坏,间隙可见少量脂肪细胞浸润。各组腓肠肌Wnt5a、GSK3、β-catenin的mRNA表达与蛋白表达比较,差异均有统计学意义(P<0.001)。结论腓肠肌损伤组织病理变化随着时间推移会逐渐恢复,按揉法刺激了Wnt5a、GSK3、β-catenin的mRNA的表达活性,从而可能减缓支架蛋白复合物(GSK3为其重要的组成部分)对β-catenin蛋白的降解,使β-catenin的蛋白水平始终维持在稳定范围,使得按揉法干预后的腓肠肌中脂肪变性减少,促进了损伤骨骼肌功能修复。展开更多
基金Chinese Ministry of Science and Technology and National Natural Science Foundation Under Grant No. 2006DFB71680
文摘It is well known that in most cases, a reference is necessary for structural health diagnosis, and it is very difficult to obtain such a reference for a given structure. In this paper, a clan member signal method （CMSM） is proposed for use in structures consisting of groups （or clans） that have the same geometry, i.e., the same cross section and length, and identical boundary conditions. It is expected that signals measured on any undamaged member in a clan after an event could be used as a reference for any other members in the clan. To verify the applicability of the proposed method, a steel truss model is tested and the results show that the CMSM is very effective in detecting local damage in structures composed of identical slender members.
