Based on the analysis of the basic characteristics of medium-and large-sized marine gas fields in Sichuan Basin, combined with the division of major reservoir forming geological units in the marine craton stage and th...Based on the analysis of the basic characteristics of medium-and large-sized marine gas fields in Sichuan Basin, combined with the division of major reservoir forming geological units in the marine craton stage and their control on key hydrocarbon accumulation factors, the distribution law of medium-and large-sized marine carbonate gas fields in the basin was examined and the exploration direction was pointed out. Through the analysis of the periodic stretching-uplifting background, it is concluded that five large scale paleo-rifts, three large scale paleo-uplifts, five large scale paleo erosion surfaces were formed in the marine craton stage of Sichuan Basin, and these geological units control the key reservoir forming factors of medium and large sized gas fields:(1) Large-scale paleo-rifts control the distribution of high-quality hydrocarbon generation centers.(2) The margin of large-scale paleo-rifts, high position of paleo-uplifts and paleo erosion surfaces control the distribution of high-quality reservoirs.(3) Large-scale paleo-rifts, paleo-uplifts, paleo erosion surfaces and present tectonic setting jointly control the formation of many types of large and medium-sized traps.(4) Natural gas accumulation is controlled by the inheritance evolution of traps in large geological units. Based on the comparative analysis of the distribution characteristics of medium-and large-sized gas fields and large geological units, it is proposed that the superimposition relationship between single or multiple geological units and the present structure controls the distribution of medium-and large-sized gas fields, and the "three paleo" superimposed area is the most advantageous. According to the above rules, the main exploration fields and directions of medium-and large-sized marine carbonate gas fields in Sichuan Basin include periphery of Deyang-Anyue paleo-rift, eastern margin of Longmenshan paleo-rift, margins of Kaijiang-Liangping oceanic trough and Chengkou-western Hubei oceanic trough, the high part of the subaqueous paleo-uplifts around Central Sichuan, paleo erosion surfaces of the top boundary of Maokou Formation in eastern and southern Sichuan Basin, paleo erosion surfaces of the top boundary of the Leikoupo Formation in central and western Sichuan Basin.展开更多
Medium-and long-chain triglycerides(MLCT)rich in arachidonic acid(ARA)were synthesized via acidolysis of microbial oil(MO)with caprylic acid(CA)using bio-imprinted lipase(BIL)as a catalyst.The optimal preparation cond...Medium-and long-chain triglycerides(MLCT)rich in arachidonic acid(ARA)were synthesized via acidolysis of microbial oil(MO)with caprylic acid(CA)using bio-imprinted lipase(BIL)as a catalyst.The optimal preparation conditions for bio-imprinting were as follows:Lipozyme RM IM as the catalyst,CA as the bio-imprinting template at a concentration of 6 mg/mL,Tween 20 at 15 mg/mL,and a buffer pH of 7.Under these conditions,the initial activity of BIL was 1.99 times higher than that of the non-bioimprinted enzyme.Thermodynamic analysis showed a decrease in activation energy from 26.46 kJ/mol to 18.08 kJ/mol after bio-imprinting.The molecular docking results revealed that the imprinted template(CA)interacted with Ser-161 of Lipozyme RM IM via a hydrogen bond,and its binding was further stabilized through additional hydrogen bonding and hydrophobic interactions,thereby enhancing the enzyme's catalytic activity.Bio-imprinting had a lower binding energy and bound more tightly to substrates with shorter carbon chain lengths.The optimal conditions for MLCT synthesis were:BIL 8 wt%,60℃,CA/MO molar ratio of 2:1 and reaction time 4 h.The maximum incorporation rate of CA achieved was 27.82 mol%,and the contents of sn-2 ARA and sn-1,3CA in MLCT were 34.20 mol%and 37.44 mol%,respectively.These findings could contribute to advancing MLCT production and deepening the under-standing of the catalytic mechanism of lipase-mediated acidolysis reactions.展开更多
In this study,medium-and long-chain triacylglycerol(MLCT)structured lipids were synthesized by lipase catalyzed acidolysis of lauric acid(La)with four kinds of common edible oils of different fatty acid compositions,i...In this study,medium-and long-chain triacylglycerol(MLCT)structured lipids were synthesized by lipase catalyzed acidolysis of lauric acid(La)with four kinds of common edible oils of different fatty acid compositions,including palm oil(PO),camellia oleifera seed oil(CSO),soybean oil(SO)and flaxseed oil(FO).The lipases and reaction conditions including substrate molar ratio,reaction temperature,(time and enzyme loading)were optimized.The physicochemical properties of their MLCT products were determined.Moreover,the interaction mechanism between lipase and different MLCT molecules was revealed.The obtained optimum conditions were as follows:substrate molar ratios of 3:1(La/oil)for FO,SO,and CSO,7:1 for PO;enzyme loading of 8%,reaction temperature of 50℃,and reaction time of 6 h for PO,4 h for SO and 3 h for CSO and FO.Under the conditions,the product based on PO had the highest La content(40.37%)and MLCT yield(94.85%),followed by FO and SO,(29.86%,29.71%and 88.41%,87.55%,respectively),least on CSO(25.40%and 81.79%).The lipase established hydrogen bonds and hydrophobic interactions with triacylglycerol ligands.Triacylglycerol 1(3),2-Linolenin-3(1)-Laurin(LnLnLa,specific in FO-MLCT)exhibited strongest binding activity with Lipozyme RM.Compared with substrate oils,the corresponding MLCT structured lipids showed improved thermal behavior and significant differences in the crystallization temperature as a result of acidolysis esterification.This study developed diverse MLCTs with La,and provided an in-depth understanding of the relationships between substrate composition,interaction,and MLCT structured lipids.展开更多
Triacylglycerol(TAG)components in human milk,infant formulas with different fat sources,and plant oils(palm oil,flaxseed oil,sunflower oil,corn oil,soybean oil,coconut oil,low erucic acid rapeseed oil,and high oleic a...Triacylglycerol(TAG)components in human milk,infant formulas with different fat sources,and plant oils(palm oil,flaxseed oil,sunflower oil,corn oil,soybean oil,coconut oil,low erucic acid rapeseed oil,and high oleic acid rapeseed oil)were analyzed and compared using ultraperformance supercritical fluid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPSFC-Q-TOF-MS).Distinct TAG profiles were identified by lipidomics,principal component analysis(PCA),and heatmap visualization.Human milk was characterized by a high abundance of medium-and long-chain triacylglycerols(MLCTs),saturated-unsaturated-unsaturated(SUU)-type TAGs(sn-2 palmitic acid).In contrast,plant oils lacked MLCTs and SUU-type TAGs,containing mainly TAGs esterified long-chain polyunsaturated fatty acid.Breast milk contains about 27%MLCT,about 50%SUU-type triacylglycerols(which contain about 34%UPU-type triacylglycerols),which is significantly different from plant oils,structured fats and infant formulas.These findings provide a clear basis for optimizing the fat blend in infant formula to better mimic the unique TAG profile of human milk,thereby improving energy delivery and nutrient absorption for infants.展开更多
Medium-and long-chain triacylglycerols(MLCT)rich in arachidonic acid(ARA)at sn-2 position were synthesized by a two-step enzymatic method.Firstly,sn-2 monoacylglycerols(MAG)were synthesized at a temperature of 25℃ by...Medium-and long-chain triacylglycerols(MLCT)rich in arachidonic acid(ARA)at sn-2 position were synthesized by a two-step enzymatic method.Firstly,sn-2 monoacylglycerols(MAG)were synthesized at a temperature of 25℃ by enzymatic alcoholysis.The MAG with 69.42%ARA at sn-2 position were obtained by solvent extraction and low temperature solvent crystallization.Secondly,the MLCT rich in ARA at sn-2 position and capric acid(CA)at sn-1,3 positions were produced by enzymatic esterification.Under the optimal conditions(MAG:CA=1:3(mol/mol),0.05 MPa vacuum,8% Lipozyme RM IM,5 h,25℃),the content of triacylglycerol was up to 93.60%.The triacylglycerol in the form of C10:0-C20:4-C10:0(including isomers)was about 40.43%.The ARA contents in the total and sn-2 fatty acid composition of the final product were 32.35%and 51.12%,respectively.MLCT rich in ARA at sn-2 position were successfully produced and the product has the potential application for functional food and infant formula.展开更多
Medium-and long-chain structured lipids(MLSLs)rich in docosahexaenoic acid(DHA)and eicosapentaenoic acid(EPA)were synthesized by acidolysis of fish oil(Omax 1812,a mixture of sardine and anchovy oils)with caprylic aci...Medium-and long-chain structured lipids(MLSLs)rich in docosahexaenoic acid(DHA)and eicosapentaenoic acid(EPA)were synthesized by acidolysis of fish oil(Omax 1812,a mixture of sardine and anchovy oils)with caprylic acid(CA)using a lipase bio-imprinted with fatty acids as a catalyst.A bio-imprinted lipase for the acidolysis reaction was first prepared under optimal conditions,and its activity was 1.43 times higher than that of the non-bioimprinted.The bio-imprinted lipase was used as a catalyst to produce such MLSLs,and the reaction conditions were optimized.Under the optimal conditions,equilibrium was achieved in 8 h,and the total and the sn-1,3 CA contents in the obtained product were 28.72%and 42.26%.Additionally,the total and the sn-2 DHA contents were 14.23%and 21.10%,respectively,and the total and the sn-2 EPA contents were 16.43%and 10.14%,respectively.These obtained MLSLs,rich in sn-1,3 CA,and sn-2 DHA,EPA can deliver significant health benefits.展开更多
More than a half of strong earthquakes in the world are located in shallow depth at the subducting plate boundary in squeezed zones. Owing to the difference in speed between the moving sea plates, the strain energy is...More than a half of strong earthquakes in the world are located in shallow depth at the subducting plate boundary in squeezed zones. Owing to the difference in speed between the moving sea plates, the strain energy is accumulated and released cyclically in squeezed zones. Several methods were developed to analyze the medium-and short-term potential of main shocks. These methods can be classified into (1) less data systems using the theory of grey model for earthquake prediction, (2) quasi-periodic systems using earthquake activity analysis, (3) systems of grouped activity using order analysis, and (4) nonlinear systems using back propagation (BP) of neural network for prediction analysis. Based upon these analytic methods, risk maps for the prediction of strong earthquakes can be drawn using the records of strong earthquakes in Taiwan for the past 100 years. These risk maps include (1) a seismic risk map, (2) a loss risk map, (3) a hazard degree map, and (4) a loss degree map. These risk maps make it possible to do a medium-term prediction of main shocks on the 10-year scale.展开更多
Type 2 diabetes mellitus(T2DM)represents a metabolic paradox of“starvation in abundance,”necessitating a reliance on dietary lipids for energy.However,current fat consumption guidelines are oversimplified and largel...Type 2 diabetes mellitus(T2DM)represents a metabolic paradox of“starvation in abundance,”necessitating a reliance on dietary lipids for energy.However,current fat consumption guidelines are oversimplified and largely overlook the distinct metabolic consequences of different glyceride structures.This review critically re-evaluates dietary fat strategies and proposes a novel framework based on three principles:1)ameliorating insulin resis-tance,2)minimizing lipid-induced metabolic burden,and 3)controlling ketone body(KB)production-a key risk factor that is often disregarded.Specifically,medium-and long-chain triglycerides(MLCTs)align with these principles through their unique structural properties.They minimize ectopic fat accumulation via the rapid oxidation of medium-chain fatty acids(MCFAs),enhance insulin sensitivity through the synergistic activation of GPR40 and insulin signaling pathways,and attenuate ketogenic potential by modulating the hepatic influx of MCFAs through the presence of long-chain fatty acids(LCFAs).Collectively,MLCTs can serve as a mechanistic foundation for developing next-generation functional lipids toward personalized T2DM management.展开更多
Medium-and long-chain triglycerides(MLCT)have been widely concerned.In this study,lauric acid(La)was incorporated into flaxseed(Linum usitatissimum L.)oil(FO,aLna-riched)by lipase-catalyzed acidolysis.The synthesis ef...Medium-and long-chain triglycerides(MLCT)have been widely concerned.In this study,lauric acid(La)was incorporated into flaxseed(Linum usitatissimum L.)oil(FO,aLna-riched)by lipase-catalyzed acidolysis.The synthesis efficiency of four immobilized lipases(Lipozyme RM IM,TL IM,AOAB8 and Novozym 435)was compared,while Lipozyme AOAB8 reaction for 2 h was the best scheme.Further,the optimal conditions for synthesizing structural lipids(aLSLs,rich in aLna and La)were obtained by response surface methodology:the reaction temperature was 44.6℃,substrate molar ratio was 12(La/FO),and enzyme loading was 9.11%.After purification by molecular distillation,the triglycerides(TAGs)of aLSLs contained 75.35% of TAGs withα-linolenic acid(aLna),40.82%of MLCTs with La,and 26.75%(65.53% of the total MLCTs)of TAGs with both aLna and La(ɑ-MLCT).Meanwhile,the content of La and aLna in aLSLs were 22.38%and 39.78%,respectively.The chemical structure and microstructure of aLSLs were similar to FO.In addition,the thermo-oxidative decomposition behavior of aLSLs was significantly improved compared with physical mixture oil.This improvement might be related to the insertion of La and the structure of TAG.In all,this study firstly developed a novel thermo-oxidatively stable MLCT with high levels of aLna and La.展开更多
基金Supported by the China National Science and Technology Major Project(2016ZX05007004,2016ZX05004005)
文摘Based on the analysis of the basic characteristics of medium-and large-sized marine gas fields in Sichuan Basin, combined with the division of major reservoir forming geological units in the marine craton stage and their control on key hydrocarbon accumulation factors, the distribution law of medium-and large-sized marine carbonate gas fields in the basin was examined and the exploration direction was pointed out. Through the analysis of the periodic stretching-uplifting background, it is concluded that five large scale paleo-rifts, three large scale paleo-uplifts, five large scale paleo erosion surfaces were formed in the marine craton stage of Sichuan Basin, and these geological units control the key reservoir forming factors of medium and large sized gas fields:(1) Large-scale paleo-rifts control the distribution of high-quality hydrocarbon generation centers.(2) The margin of large-scale paleo-rifts, high position of paleo-uplifts and paleo erosion surfaces control the distribution of high-quality reservoirs.(3) Large-scale paleo-rifts, paleo-uplifts, paleo erosion surfaces and present tectonic setting jointly control the formation of many types of large and medium-sized traps.(4) Natural gas accumulation is controlled by the inheritance evolution of traps in large geological units. Based on the comparative analysis of the distribution characteristics of medium-and large-sized gas fields and large geological units, it is proposed that the superimposition relationship between single or multiple geological units and the present structure controls the distribution of medium-and large-sized gas fields, and the "three paleo" superimposed area is the most advantageous. According to the above rules, the main exploration fields and directions of medium-and large-sized marine carbonate gas fields in Sichuan Basin include periphery of Deyang-Anyue paleo-rift, eastern margin of Longmenshan paleo-rift, margins of Kaijiang-Liangping oceanic trough and Chengkou-western Hubei oceanic trough, the high part of the subaqueous paleo-uplifts around Central Sichuan, paleo erosion surfaces of the top boundary of Maokou Formation in eastern and southern Sichuan Basin, paleo erosion surfaces of the top boundary of the Leikoupo Formation in central and western Sichuan Basin.
基金supported by the National Natural Science Foundation of China(31601433)。
文摘Medium-and long-chain triglycerides(MLCT)rich in arachidonic acid(ARA)were synthesized via acidolysis of microbial oil(MO)with caprylic acid(CA)using bio-imprinted lipase(BIL)as a catalyst.The optimal preparation conditions for bio-imprinting were as follows:Lipozyme RM IM as the catalyst,CA as the bio-imprinting template at a concentration of 6 mg/mL,Tween 20 at 15 mg/mL,and a buffer pH of 7.Under these conditions,the initial activity of BIL was 1.99 times higher than that of the non-bioimprinted enzyme.Thermodynamic analysis showed a decrease in activation energy from 26.46 kJ/mol to 18.08 kJ/mol after bio-imprinting.The molecular docking results revealed that the imprinted template(CA)interacted with Ser-161 of Lipozyme RM IM via a hydrogen bond,and its binding was further stabilized through additional hydrogen bonding and hydrophobic interactions,thereby enhancing the enzyme's catalytic activity.Bio-imprinting had a lower binding energy and bound more tightly to substrates with shorter carbon chain lengths.The optimal conditions for MLCT synthesis were:BIL 8 wt%,60℃,CA/MO molar ratio of 2:1 and reaction time 4 h.The maximum incorporation rate of CA achieved was 27.82 mol%,and the contents of sn-2 ARA and sn-1,3CA in MLCT were 34.20 mol%and 37.44 mol%,respectively.These findings could contribute to advancing MLCT production and deepening the under-standing of the catalytic mechanism of lipase-mediated acidolysis reactions.
基金supported by the National Natural Science Foundation of China(32372469,32302223 and 31871763)Basic Scientific Research Project of Zhejiang University of Science and Technology(2023QN032)+1 种基金Zhejiang Province Key Science and Technology Projects(2023C02042,2023C02045)Agriculture and Social Development Projects of Hangzhou(202203A11).
文摘In this study,medium-and long-chain triacylglycerol(MLCT)structured lipids were synthesized by lipase catalyzed acidolysis of lauric acid(La)with four kinds of common edible oils of different fatty acid compositions,including palm oil(PO),camellia oleifera seed oil(CSO),soybean oil(SO)and flaxseed oil(FO).The lipases and reaction conditions including substrate molar ratio,reaction temperature,(time and enzyme loading)were optimized.The physicochemical properties of their MLCT products were determined.Moreover,the interaction mechanism between lipase and different MLCT molecules was revealed.The obtained optimum conditions were as follows:substrate molar ratios of 3:1(La/oil)for FO,SO,and CSO,7:1 for PO;enzyme loading of 8%,reaction temperature of 50℃,and reaction time of 6 h for PO,4 h for SO and 3 h for CSO and FO.Under the conditions,the product based on PO had the highest La content(40.37%)and MLCT yield(94.85%),followed by FO and SO,(29.86%,29.71%and 88.41%,87.55%,respectively),least on CSO(25.40%and 81.79%).The lipase established hydrogen bonds and hydrophobic interactions with triacylglycerol ligands.Triacylglycerol 1(3),2-Linolenin-3(1)-Laurin(LnLnLa,specific in FO-MLCT)exhibited strongest binding activity with Lipozyme RM.Compared with substrate oils,the corresponding MLCT structured lipids showed improved thermal behavior and significant differences in the crystallization temperature as a result of acidolysis esterification.This study developed diverse MLCTs with La,and provided an in-depth understanding of the relationships between substrate composition,interaction,and MLCT structured lipids.
基金supported by the National Key Research and Development Program of China(2021YFD2100700).
文摘Triacylglycerol(TAG)components in human milk,infant formulas with different fat sources,and plant oils(palm oil,flaxseed oil,sunflower oil,corn oil,soybean oil,coconut oil,low erucic acid rapeseed oil,and high oleic acid rapeseed oil)were analyzed and compared using ultraperformance supercritical fluid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPSFC-Q-TOF-MS).Distinct TAG profiles were identified by lipidomics,principal component analysis(PCA),and heatmap visualization.Human milk was characterized by a high abundance of medium-and long-chain triacylglycerols(MLCTs),saturated-unsaturated-unsaturated(SUU)-type TAGs(sn-2 palmitic acid).In contrast,plant oils lacked MLCTs and SUU-type TAGs,containing mainly TAGs esterified long-chain polyunsaturated fatty acid.Breast milk contains about 27%MLCT,about 50%SUU-type triacylglycerols(which contain about 34%UPU-type triacylglycerols),which is significantly different from plant oils,structured fats and infant formulas.These findings provide a clear basis for optimizing the fat blend in infant formula to better mimic the unique TAG profile of human milk,thereby improving energy delivery and nutrient absorption for infants.
基金supported by the National Natural Science Foundation of China(31601433)Jiangsu Province Natural Science Foundation(BK20140149).
文摘Medium-and long-chain triacylglycerols(MLCT)rich in arachidonic acid(ARA)at sn-2 position were synthesized by a two-step enzymatic method.Firstly,sn-2 monoacylglycerols(MAG)were synthesized at a temperature of 25℃ by enzymatic alcoholysis.The MAG with 69.42%ARA at sn-2 position were obtained by solvent extraction and low temperature solvent crystallization.Secondly,the MLCT rich in ARA at sn-2 position and capric acid(CA)at sn-1,3 positions were produced by enzymatic esterification.Under the optimal conditions(MAG:CA=1:3(mol/mol),0.05 MPa vacuum,8% Lipozyme RM IM,5 h,25℃),the content of triacylglycerol was up to 93.60%.The triacylglycerol in the form of C10:0-C20:4-C10:0(including isomers)was about 40.43%.The ARA contents in the total and sn-2 fatty acid composition of the final product were 32.35%and 51.12%,respectively.MLCT rich in ARA at sn-2 position were successfully produced and the product has the potential application for functional food and infant formula.
基金supported by the National Natural Science Foundation of China(31601433).
文摘Medium-and long-chain structured lipids(MLSLs)rich in docosahexaenoic acid(DHA)and eicosapentaenoic acid(EPA)were synthesized by acidolysis of fish oil(Omax 1812,a mixture of sardine and anchovy oils)with caprylic acid(CA)using a lipase bio-imprinted with fatty acids as a catalyst.A bio-imprinted lipase for the acidolysis reaction was first prepared under optimal conditions,and its activity was 1.43 times higher than that of the non-bioimprinted.The bio-imprinted lipase was used as a catalyst to produce such MLSLs,and the reaction conditions were optimized.Under the optimal conditions,equilibrium was achieved in 8 h,and the total and the sn-1,3 CA contents in the obtained product were 28.72%and 42.26%.Additionally,the total and the sn-2 DHA contents were 14.23%and 21.10%,respectively,and the total and the sn-2 EPA contents were 16.43%and 10.14%,respectively.These obtained MLSLs,rich in sn-1,3 CA,and sn-2 DHA,EPA can deliver significant health benefits.
文摘More than a half of strong earthquakes in the world are located in shallow depth at the subducting plate boundary in squeezed zones. Owing to the difference in speed between the moving sea plates, the strain energy is accumulated and released cyclically in squeezed zones. Several methods were developed to analyze the medium-and short-term potential of main shocks. These methods can be classified into (1) less data systems using the theory of grey model for earthquake prediction, (2) quasi-periodic systems using earthquake activity analysis, (3) systems of grouped activity using order analysis, and (4) nonlinear systems using back propagation (BP) of neural network for prediction analysis. Based upon these analytic methods, risk maps for the prediction of strong earthquakes can be drawn using the records of strong earthquakes in Taiwan for the past 100 years. These risk maps include (1) a seismic risk map, (2) a loss risk map, (3) a hazard degree map, and (4) a loss degree map. These risk maps make it possible to do a medium-term prediction of main shocks on the 10-year scale.
基金supported by the National Key Research and Devel-opment Project of China(2021YFD2100303).
文摘Type 2 diabetes mellitus(T2DM)represents a metabolic paradox of“starvation in abundance,”necessitating a reliance on dietary lipids for energy.However,current fat consumption guidelines are oversimplified and largely overlook the distinct metabolic consequences of different glyceride structures.This review critically re-evaluates dietary fat strategies and proposes a novel framework based on three principles:1)ameliorating insulin resis-tance,2)minimizing lipid-induced metabolic burden,and 3)controlling ketone body(KB)production-a key risk factor that is often disregarded.Specifically,medium-and long-chain triglycerides(MLCTs)align with these principles through their unique structural properties.They minimize ectopic fat accumulation via the rapid oxidation of medium-chain fatty acids(MCFAs),enhance insulin sensitivity through the synergistic activation of GPR40 and insulin signaling pathways,and attenuate ketogenic potential by modulating the hepatic influx of MCFAs through the presence of long-chain fatty acids(LCFAs).Collectively,MLCTs can serve as a mechanistic foundation for developing next-generation functional lipids toward personalized T2DM management.
基金The authors gratefully acknowledge the funding support of Key Project of Science and Technology Innovation 2025 program(2019B10060)the National Natural Science Foundation of China(32072224).
文摘Medium-and long-chain triglycerides(MLCT)have been widely concerned.In this study,lauric acid(La)was incorporated into flaxseed(Linum usitatissimum L.)oil(FO,aLna-riched)by lipase-catalyzed acidolysis.The synthesis efficiency of four immobilized lipases(Lipozyme RM IM,TL IM,AOAB8 and Novozym 435)was compared,while Lipozyme AOAB8 reaction for 2 h was the best scheme.Further,the optimal conditions for synthesizing structural lipids(aLSLs,rich in aLna and La)were obtained by response surface methodology:the reaction temperature was 44.6℃,substrate molar ratio was 12(La/FO),and enzyme loading was 9.11%.After purification by molecular distillation,the triglycerides(TAGs)of aLSLs contained 75.35% of TAGs withα-linolenic acid(aLna),40.82%of MLCTs with La,and 26.75%(65.53% of the total MLCTs)of TAGs with both aLna and La(ɑ-MLCT).Meanwhile,the content of La and aLna in aLSLs were 22.38%and 39.78%,respectively.The chemical structure and microstructure of aLSLs were similar to FO.In addition,the thermo-oxidative decomposition behavior of aLSLs was significantly improved compared with physical mixture oil.This improvement might be related to the insertion of La and the structure of TAG.In all,this study firstly developed a novel thermo-oxidatively stable MLCT with high levels of aLna and La.
