The exogenous gene was integrated into Dunaliella salina successfully by using LiAc/PEG mediating method for the first time. According to the results of histochemical staining, transgenic D. salina was blue, showing t...The exogenous gene was integrated into Dunaliella salina successfully by using LiAc/PEG mediating method for the first time. According to the results of histochemical staining, transgenic D. salina was blue, showing that the exogenous GUS gene was successfully expressed in the cells of D. salina. Meanwhile, the effects of growth state of D. salina, plasmid concentration and temperature on its transformation efficiency were studied, and the transformation conditions were optimized. The results show that the optimum conditions for the genetic transformation of D. salina are shown as follows： D. salina was in the early logarithmic phase; plasmid DNA concentration was 600 μg/ml; temperature was 29 ℃, and transformation efficiency was up to 74.8‰ under the best conditions. According to the results of PCR amplification and PCR-Southern hybridization, the target gene had been integrated into genome and was hereditary.展开更多
The NaGd(WO4)2:Eu3+ phosphors were prepared by the microemulsion mediated hydrothermal method. The morphology and size of the samples could be dependent on the pH in the initial solution. These phosphors obtained had ...The NaGd(WO4)2:Eu3+ phosphors were prepared by the microemulsion mediated hydrothermal method. The morphology and size of the samples could be dependent on the pH in the initial solution. These phosphors obtained had a strong absorption at 395 nm matching with the popular emission of near-UV LED chips, and could emit intense red light at 616 nm. The small ellipsoid-like particles or short rods were provided with large parameters of oscillator strength?2 and asymmetry ratio. Compared to the ellip-soid-like phosphors, the rod-like phosphors took on stronger emission, longer lifetime and larger quantum efficiency. This work demonstrated that the one-dimension NaGd(WO4)2:Eu3+ phosphors might be a more favorable device than zero-dimension ones for photoluminescence.展开更多
Plasmonic nanoparticles are endowed profound capability for sensing,biomedicine,and cancer therapy.However,the inaccessibly adjustable wavelength in near infrared(NIR)region window and size limit for the particles pen...Plasmonic nanoparticles are endowed profound capability for sensing,biomedicine,and cancer therapy.However,the inaccessibly adjustable wavelength in near infrared(NIR)region window and size limit for the particles penetration in tumor strongly hinder their developments.Miniature gold nanorods(mini-Au NRs)with diameter less than 12 nm can effectively address this challenge due to the tiny size and tailorable NIR absorption.Herein,we adopt ternary surfactants(hexadecyl trimethyl ammonium bromide(CTAB),sodium oleate(NaOL),and sodium salicylate(NaSal))mediated growth strategy to precisely synthesize miniature Au NRs under micelle space-confinement.Importantly,the selectively dense accumulation of ternary surfactants can efficiently improve the micellar stacking parameters(p)and lower micellar free energy(F),further tends to achieve the formation of Au NRs with tiny diameter and high purity.Compared with that of conventional methods,the purity of mini-Au NRs up to 100%can be dramatically improved via varying the relative concentration of ternary surfactants.The diameter of Au NRs can be dynamically controlled to 6,8,and 11 nm through regulating the concentration of silver nitrate and the mole ratio of ternary surfactants.Such ternary surfactants system is favorable for the aging of tiny Au NRs,and further enables the aspect ratio-tunable in the region from 2.70 to 7.32,as well as tailorable plasmonic wavelength in wide NIR window from 700 to 1,147 nm.Therefore,our findings shed a light on the precise preparation of small sized plasmonic nanoparticles and pave the way to applications in biomedicine,imaging,and cancer therapy.展开更多
Background To investigate the relationship of age at first separation from parents(AFSP)with suicidal ideation(SI),suicide planning(SP)and suicide attempts(SA)among left-behind children(LBC)in China and the mediating ...Background To investigate the relationship of age at first separation from parents(AFSP)with suicidal ideation(SI),suicide planning(SP)and suicide attempts(SA)among left-behind children(LBC)in China and the mediating effect of traumatic events(TEs).Methods This cross-sectional study employed a multistage sampling method to conduct questionnaire surveys in six high-concentrated LBC provinces.Logistic regression and restricted cubic splines were performed to investigate the association of AFSP with SI,SP and SA,and meta-analysis(I2)was used to assess the heterogeneity.Causal mediation analysis(structural equation modelling)was used to evaluate the mediating effect of TEs,and bootstrap resampling was employed to assess the robustness of the causal mediation analysis.Results A total of 12162(6269 LBC)participants were included in this study;the prevalence rates of SI,SP and SA among LBC were 30.7%,18.6%and 11.9%;and LBC had a higher risk of SI(OR:1.32),SP(OR:1.40)and SA(OR:1.25)than non-LBC,all p<0.001.AFSP was negatively associated with SI,SP and SA,particularly peaking before 2 years old.LBC with AFSP<2 years old had a higher risk of SI(OR 2.65;95%CI 2.17 to 3.24;p<0.001),SP(OR 2.63;95%CI 3.36 to 4.29;p<0.001)and SA(OR 2.04;95%CI 2.73 to 3.63;p<0.001).TEs mediated the increased risk of SI(average causal mediation effect(ACME)0.03–0.07;proportion(Prop)40–54%;all p<0.001),SP(ACME 0.03–0.06;Prop 39–61%;all p<0.001)and SA(ACME 0.02–0.04;Prop 53–80%;all p<0.01).Conclusions We found that AFSP was associated with SI,SP and SA,mediated by TEs.Our findings supported the necessity of reducing SI,SP and SA risks among LBC and highlighted the need to consider AFSPs and TEs in intervention strategies.展开更多
基金Supported by National Natural Science Foundation of China(31472260)~~
文摘The exogenous gene was integrated into Dunaliella salina successfully by using LiAc/PEG mediating method for the first time. According to the results of histochemical staining, transgenic D. salina was blue, showing that the exogenous GUS gene was successfully expressed in the cells of D. salina. Meanwhile, the effects of growth state of D. salina, plasmid concentration and temperature on its transformation efficiency were studied, and the transformation conditions were optimized. The results show that the optimum conditions for the genetic transformation of D. salina are shown as follows： D. salina was in the early logarithmic phase; plasmid DNA concentration was 600 μg/ml; temperature was 29 ℃, and transformation efficiency was up to 74.8‰ under the best conditions. According to the results of PCR amplification and PCR-Southern hybridization, the target gene had been integrated into genome and was hereditary.
基金supported by the National Natural Science Foundation of China(61565006,61204003,51563009 and 11547229)Train Object Program of Jiangxi Province Young Scientists,Natural Science Foundation of Jiangxi Province(2011BAB206029,20142BAB216011,2015BAB213015)the Excellent Young Academic Talent Program of Jiangxi University of Finance and Economics
文摘The NaGd(WO4)2:Eu3+ phosphors were prepared by the microemulsion mediated hydrothermal method. The morphology and size of the samples could be dependent on the pH in the initial solution. These phosphors obtained had a strong absorption at 395 nm matching with the popular emission of near-UV LED chips, and could emit intense red light at 616 nm. The small ellipsoid-like particles or short rods were provided with large parameters of oscillator strength?2 and asymmetry ratio. Compared to the ellip-soid-like phosphors, the rod-like phosphors took on stronger emission, longer lifetime and larger quantum efficiency. This work demonstrated that the one-dimension NaGd(WO4)2:Eu3+ phosphors might be a more favorable device than zero-dimension ones for photoluminescence.
基金the financial support from the National Natural Science Foundation of China(Nos.52222316,52103325,and 52111530128)the Zhejiang Provincial Natural Science Foundation of China(No.Z22B050001)+1 种基金Ten Thousand People Plan of Zhejiang Province(No.2019R51012)China Postdoctoral Science Foundation(No.2022M713020).
文摘Plasmonic nanoparticles are endowed profound capability for sensing,biomedicine,and cancer therapy.However,the inaccessibly adjustable wavelength in near infrared(NIR)region window and size limit for the particles penetration in tumor strongly hinder their developments.Miniature gold nanorods(mini-Au NRs)with diameter less than 12 nm can effectively address this challenge due to the tiny size and tailorable NIR absorption.Herein,we adopt ternary surfactants(hexadecyl trimethyl ammonium bromide(CTAB),sodium oleate(NaOL),and sodium salicylate(NaSal))mediated growth strategy to precisely synthesize miniature Au NRs under micelle space-confinement.Importantly,the selectively dense accumulation of ternary surfactants can efficiently improve the micellar stacking parameters(p)and lower micellar free energy(F),further tends to achieve the formation of Au NRs with tiny diameter and high purity.Compared with that of conventional methods,the purity of mini-Au NRs up to 100%can be dramatically improved via varying the relative concentration of ternary surfactants.The diameter of Au NRs can be dynamically controlled to 6,8,and 11 nm through regulating the concentration of silver nitrate and the mole ratio of ternary surfactants.Such ternary surfactants system is favorable for the aging of tiny Au NRs,and further enables the aspect ratio-tunable in the region from 2.70 to 7.32,as well as tailorable plasmonic wavelength in wide NIR window from 700 to 1,147 nm.Therefore,our findings shed a light on the precise preparation of small sized plasmonic nanoparticles and pave the way to applications in biomedicine,imaging,and cancer therapy.
基金supported by the National Social Science Fund of China(NSSFC)(Grant No 20BSH066,JZ).
文摘Background To investigate the relationship of age at first separation from parents(AFSP)with suicidal ideation(SI),suicide planning(SP)and suicide attempts(SA)among left-behind children(LBC)in China and the mediating effect of traumatic events(TEs).Methods This cross-sectional study employed a multistage sampling method to conduct questionnaire surveys in six high-concentrated LBC provinces.Logistic regression and restricted cubic splines were performed to investigate the association of AFSP with SI,SP and SA,and meta-analysis(I2)was used to assess the heterogeneity.Causal mediation analysis(structural equation modelling)was used to evaluate the mediating effect of TEs,and bootstrap resampling was employed to assess the robustness of the causal mediation analysis.Results A total of 12162(6269 LBC)participants were included in this study;the prevalence rates of SI,SP and SA among LBC were 30.7%,18.6%and 11.9%;and LBC had a higher risk of SI(OR:1.32),SP(OR:1.40)and SA(OR:1.25)than non-LBC,all p<0.001.AFSP was negatively associated with SI,SP and SA,particularly peaking before 2 years old.LBC with AFSP<2 years old had a higher risk of SI(OR 2.65;95%CI 2.17 to 3.24;p<0.001),SP(OR 2.63;95%CI 3.36 to 4.29;p<0.001)and SA(OR 2.04;95%CI 2.73 to 3.63;p<0.001).TEs mediated the increased risk of SI(average causal mediation effect(ACME)0.03–0.07;proportion(Prop)40–54%;all p<0.001),SP(ACME 0.03–0.06;Prop 39–61%;all p<0.001)and SA(ACME 0.02–0.04;Prop 53–80%;all p<0.01).Conclusions We found that AFSP was associated with SI,SP and SA,mediated by TEs.Our findings supported the necessity of reducing SI,SP and SA risks among LBC and highlighted the need to consider AFSPs and TEs in intervention strategies.
