A series of biodegradable nanoparticle-based drug delivery systems have been designed utilizing poly(β-amino ester)-guanidine-phenylboronic acid(PBAE-G)polymers.In this study,a novel Lentinan-Functionalized PBAE-G-na...A series of biodegradable nanoparticle-based drug delivery systems have been designed utilizing poly(β-amino ester)-guanidine-phenylboronic acid(PBAE-G)polymers.In this study,a novel Lentinan-Functionalized PBAE-G-nanodiamond system was developed to carry ovalbumin(LNT-PBAE-G-ND@OVA).The impact of this drug delivery system on the activation and maturation of macrophages was then assessed.Furthermore,LNT-PBAE-G-ND@OVA induced potent antibody response and showed no obvious toxicity in vitro and in vivo.Moreover,treatment with LNT-PBAE-G-ND@OVA was sufficient to alter the expression of genes associated with the cGAS-STING pathway,and the LNT-PBAE-G-ND@OVA induced upregulation of costimulatory molecules.LNT-PBAE-G-ND@OVA treatment was sufficient to induce macrophage activation through a complex mechanism in which cyclic guanosine monophosphate-adenosine monophosphate(cGAMP)synthase(cGAS)-stimulator of interferon genes(STING)signaling plays an integral role.展开更多
文摘A series of biodegradable nanoparticle-based drug delivery systems have been designed utilizing poly(β-amino ester)-guanidine-phenylboronic acid(PBAE-G)polymers.In this study,a novel Lentinan-Functionalized PBAE-G-nanodiamond system was developed to carry ovalbumin(LNT-PBAE-G-ND@OVA).The impact of this drug delivery system on the activation and maturation of macrophages was then assessed.Furthermore,LNT-PBAE-G-ND@OVA induced potent antibody response and showed no obvious toxicity in vitro and in vivo.Moreover,treatment with LNT-PBAE-G-ND@OVA was sufficient to alter the expression of genes associated with the cGAS-STING pathway,and the LNT-PBAE-G-ND@OVA induced upregulation of costimulatory molecules.LNT-PBAE-G-ND@OVA treatment was sufficient to induce macrophage activation through a complex mechanism in which cyclic guanosine monophosphate-adenosine monophosphate(cGAMP)synthase(cGAS)-stimulator of interferon genes(STING)signaling plays an integral role.
