Xyloglucan represents the primary hemicellulose component in higher plant cell walls,providing mechanical support.The XTH gene family encodes xyloglucan endotransferase/hydrolase,a crucial enzyme in cell wall remodeli...Xyloglucan represents the primary hemicellulose component in higher plant cell walls,providing mechanical support.The XTH gene family encodes xyloglucan endotransferase/hydrolase,a crucial enzyme in cell wall remodeling.Studies examining XTH family-related genes in apples remain limited.This study investigated the Md XTH30 gene,isolated from apple(Malus×domestica),which demonstrated responsiveness to abscisic acid,Na Cl,and polyethylene glycol(PEG)6000,with cytoplasmic localization confirmed through subcellular mapping.To elucidate the role of Md XTH30 in stress response,transgenic Md XTH30 apple calli were generated and the gene was heterologously expressed in Arabidopsis via Agrobacterium-mediated transformation.The findings revealed that Md XTH30 enhanced resistance to drought,salt stress,and pathogens through regulation of relevant genes in both apple calli and Arabidopsis.These results identify potentially significant candidate genes for improving biotic and abiotic stress resistance at the cell wall level.展开更多
In our recently published paper,[1]a typesetting error occurred during the production process.Figure 1 in the published version was incomplete.The processing of molecular dynamics(MD)simulation data into graph-structu...In our recently published paper,[1]a typesetting error occurred during the production process.Figure 1 in the published version was incomplete.The processing of molecular dynamics(MD)simulation data into graph-structured representations in the left bottom panel of thefigure was inadvertently omitted.展开更多
[目的]丙泊酚改善重症脓毒症小鼠脑保护作用的研究。[方法]将48只雄性小鼠随机分为对照组、LPS组、PF组、PF+ML385组,每组12只。通过苏木素-伊红染色评估各组小鼠海马组织的病理变化;通过原位末端标记法分析海马组织中神经元的凋亡率;...[目的]丙泊酚改善重症脓毒症小鼠脑保护作用的研究。[方法]将48只雄性小鼠随机分为对照组、LPS组、PF组、PF+ML385组,每组12只。通过苏木素-伊红染色评估各组小鼠海马组织的病理变化;通过原位末端标记法分析海马组织中神经元的凋亡率;通过酶联免疫吸附实验分析血清中TNF-α、IL-6、SOD和CAT的水平;通过蛋白免疫印迹实验分析海马组织中TLR4/MD-2的蛋白表达水平。[结果]与LPS组比较,PF组与PF+ML385组小鼠海马组织区域炎症细胞聚集减少,神经元损伤减轻,神经元凋亡率降低,血清炎症因子(TNF-α和IL-6)水平降低,血清抗氧化酶(SOD和CAT)活性水平增加,海马组织的TLR4、MD-2蛋白表达水平降低。与PF组比较,PF+ML385组小鼠海马组织神经元凋亡率降低(13.26%±2.19%vs 9.28%±1.58%;P<0.05),血清炎症因子(TNF-α、IL-6)水平降低(15.61±1.29 vs 10.28±1.46 pg/mL;30.28±1.11 vs 23.56±2.13 pg/mL;P<0.05),血清抗氧化酶(SOD、CAT)活性水平增加(13.69±1.72 vs 19.23±2.39 U/mg protein;12.97±2.26 vs 17.38±1.47 U/mg protein;P<0.05),海马组织的TLR4、MD-2蛋白表达水平降低(0.65±0.03 vs 0.33±0.09;0.72±0.08 vs 0.41±0.04;P<0.05)。[结论]丙泊酚能够改善脓毒症小鼠海马组织的神经元损伤,减少神经元凋亡率和炎症因子水平,增加抗氧化酶水平。丙泊酚的这一作用与抑制TLR4/MD-2通路密切相关。展开更多
【目的】系统探究柴达木盆地耕地耕层土壤盐碱化水平及养分状况,优化柴达木盆地耕地耕层土壤评价因子,构建评价与诊断模型,为区域粮食安全和生态可持续发展提供科学依据。【方法】以柴达木盆地耕作层土壤为对象,基于470个土壤样品的11...【目的】系统探究柴达木盆地耕地耕层土壤盐碱化水平及养分状况,优化柴达木盆地耕地耕层土壤评价因子,构建评价与诊断模型,为区域粮食安全和生态可持续发展提供科学依据。【方法】以柴达木盆地耕作层土壤为对象,基于470个土壤样品的11项土壤指标(全盐:TS、电导率:EC、全氮:TN、全磷:TP、速效氮:AN、速效磷:AP、pH、有机质:SOM、钠离子:Na^(+)、氯离子:Cl^(-)、硫酸根离子:SO_(4)^(2-)),通过主成分分析(PCA)与Norm值构建最小数据集(Minimum data set,MDS),结合多准则妥协解排序法(VIKOR法),系统评价土壤质量指数(Soil quality index,SQI)并诊断主要障碍因子。【结果】研究区土壤呈现显著盐碱化风险(TS均值2.449 g/kg,C_(V)=53.98%)和氮磷养分限制特征(TN均值为0.916 mg/kg,TP的C_(V)为118.38%);MDS筛选出TS、TN、TP、AP、Cl^(-)和SO_(4)^(2-)等6项核心指标,可替代全数据集(Total data set,TDS)精准评价土壤质量(R^(2)=0.904);VIKOR模型显示,格尔木和大柴旦地区土壤综合障碍度最高(群体效应值S>0.46),TP与SO_(4)^(2-)是主要限制因子,德令哈土壤质量最优(SQI=0.284)。【讨论】研究揭示了盐碱化与养分失衡对干旱区土壤质量的协同制约效应,为柴达木盆地耕地定向改良提供了科学依据。展开更多
基金supported by the National Key R&D Plan Project(2023YFD2301000)the National Key Research and Development Program of Shandong Province,China(2024CXGC0109032023CXPT013)+2 种基金the National Natural Science Foundation of China(32472705,32302513)the Young Talent of Lifting Engineering for Science and Technology in Shandong,China(SDAST2024QTA083)the Natural Science Foundation of Shandong Province,China(ZR2022JQ14,ZR2022QC112)。
文摘Xyloglucan represents the primary hemicellulose component in higher plant cell walls,providing mechanical support.The XTH gene family encodes xyloglucan endotransferase/hydrolase,a crucial enzyme in cell wall remodeling.Studies examining XTH family-related genes in apples remain limited.This study investigated the Md XTH30 gene,isolated from apple(Malus×domestica),which demonstrated responsiveness to abscisic acid,Na Cl,and polyethylene glycol(PEG)6000,with cytoplasmic localization confirmed through subcellular mapping.To elucidate the role of Md XTH30 in stress response,transgenic Md XTH30 apple calli were generated and the gene was heterologously expressed in Arabidopsis via Agrobacterium-mediated transformation.The findings revealed that Md XTH30 enhanced resistance to drought,salt stress,and pathogens through regulation of relevant genes in both apple calli and Arabidopsis.These results identify potentially significant candidate genes for improving biotic and abiotic stress resistance at the cell wall level.
文摘In our recently published paper,[1]a typesetting error occurred during the production process.Figure 1 in the published version was incomplete.The processing of molecular dynamics(MD)simulation data into graph-structured representations in the left bottom panel of thefigure was inadvertently omitted.
文摘[目的]丙泊酚改善重症脓毒症小鼠脑保护作用的研究。[方法]将48只雄性小鼠随机分为对照组、LPS组、PF组、PF+ML385组,每组12只。通过苏木素-伊红染色评估各组小鼠海马组织的病理变化;通过原位末端标记法分析海马组织中神经元的凋亡率;通过酶联免疫吸附实验分析血清中TNF-α、IL-6、SOD和CAT的水平;通过蛋白免疫印迹实验分析海马组织中TLR4/MD-2的蛋白表达水平。[结果]与LPS组比较,PF组与PF+ML385组小鼠海马组织区域炎症细胞聚集减少,神经元损伤减轻,神经元凋亡率降低,血清炎症因子(TNF-α和IL-6)水平降低,血清抗氧化酶(SOD和CAT)活性水平增加,海马组织的TLR4、MD-2蛋白表达水平降低。与PF组比较,PF+ML385组小鼠海马组织神经元凋亡率降低(13.26%±2.19%vs 9.28%±1.58%;P<0.05),血清炎症因子(TNF-α、IL-6)水平降低(15.61±1.29 vs 10.28±1.46 pg/mL;30.28±1.11 vs 23.56±2.13 pg/mL;P<0.05),血清抗氧化酶(SOD、CAT)活性水平增加(13.69±1.72 vs 19.23±2.39 U/mg protein;12.97±2.26 vs 17.38±1.47 U/mg protein;P<0.05),海马组织的TLR4、MD-2蛋白表达水平降低(0.65±0.03 vs 0.33±0.09;0.72±0.08 vs 0.41±0.04;P<0.05)。[结论]丙泊酚能够改善脓毒症小鼠海马组织的神经元损伤,减少神经元凋亡率和炎症因子水平,增加抗氧化酶水平。丙泊酚的这一作用与抑制TLR4/MD-2通路密切相关。
文摘【目的】系统探究柴达木盆地耕地耕层土壤盐碱化水平及养分状况,优化柴达木盆地耕地耕层土壤评价因子,构建评价与诊断模型,为区域粮食安全和生态可持续发展提供科学依据。【方法】以柴达木盆地耕作层土壤为对象,基于470个土壤样品的11项土壤指标(全盐:TS、电导率:EC、全氮:TN、全磷:TP、速效氮:AN、速效磷:AP、pH、有机质:SOM、钠离子:Na^(+)、氯离子:Cl^(-)、硫酸根离子:SO_(4)^(2-)),通过主成分分析(PCA)与Norm值构建最小数据集(Minimum data set,MDS),结合多准则妥协解排序法(VIKOR法),系统评价土壤质量指数(Soil quality index,SQI)并诊断主要障碍因子。【结果】研究区土壤呈现显著盐碱化风险(TS均值2.449 g/kg,C_(V)=53.98%)和氮磷养分限制特征(TN均值为0.916 mg/kg,TP的C_(V)为118.38%);MDS筛选出TS、TN、TP、AP、Cl^(-)和SO_(4)^(2-)等6项核心指标,可替代全数据集(Total data set,TDS)精准评价土壤质量(R^(2)=0.904);VIKOR模型显示,格尔木和大柴旦地区土壤综合障碍度最高(群体效应值S>0.46),TP与SO_(4)^(2-)是主要限制因子,德令哈土壤质量最优(SQI=0.284)。【讨论】研究揭示了盐碱化与养分失衡对干旱区土壤质量的协同制约效应,为柴达木盆地耕地定向改良提供了科学依据。
