Peanut(Arachis hypogaea L.)exhibits an unusually asynchronous reproductive cycle,in which flowering,peg penetration,pod development,and seed filling occur over an extended period.This results in the simultaneous prese...Peanut(Arachis hypogaea L.)exhibits an unusually asynchronous reproductive cycle,in which flowering,peg penetration,pod development,and seed filling occur over an extended period.This results in the simultaneous presence of immature and preharvest sprouted(PHS)pods on the same plant a dual challenge that undermines yield,compromises seed quality,and complicates postharvest management.Immature pods reduce harvest efficiency,while PHS diminishes flavor,uniformity,and storage stability.Both genetic and environmental determinants ranging from temporal variation in peg initiation and hormonal gradients to microenvironmental heterogeneity and differential seed dormancy shape this variability.However,despite advances in pod biology,systematic field-based quantification of intra-plant temporal variation,genotype×environment interactions,and localized microclimatic influences remains limited.This review aims to synthesize current understanding of within-plant variability in pod maturation and PHS in peanut,to elucidate critical knowledge gaps at physiological and field scales,and to evaluate emerging strategies for mitigation.Particular emphasis is given for underexplored interface between physiological mechanisms and field-scale dynamics.Emerging innovations including hyperspectral imaging,soil and canopy moisture sensing,and molecular markers offer promising avenues for precise monitoring of pod maturity and early detection of PHS risk.Integrating these tools with targeted breeding strategies for synchronous flowering,enhanced dormancy,and late-season stress resilience,alongside adaptive agronomic practices such as optimized sowing,irrigation scheduling,nutrient management,and harvest timing,could substantially reduce yield and quality losses.Future progress will depend on bridging molecular insights with predictive models that capture mixed maturity and sprouting risk under variable environments.展开更多
In-situ heating conversion is the most practical recovery method for lacustrine low-to-medium maturity shale oil.However,the energy output-input ratio must exceed the economic threshold to achieve commercial developme...In-situ heating conversion is the most practical recovery method for lacustrine low-to-medium maturity shale oil.However,the energy output-input ratio must exceed the economic threshold to achieve commercial development.This paper systematically investigates the mechanism of super-rich accumulation of organic matter in continental shale,sweet spot evaluation,optimal heating windows,and appropriate well types and patterns from the perspectives of enhancing energy output and reducing energy input.(1)The super-rich accumulation of organic matter in lacustrine shale is primarily controlled by the intensity,frequency,and preservation of external material inputs,and is related to moderate volcanic and hydrothermal activities,marine transgressions,with total organic carbon content greater than or equal to 6%.(2)The quality of organic-rich intervals is related to the type of source material and hydrocarbon generation potential.The in-situ conversion-derived hydrocarbon quality index(HQI)is established,and the zones exhibiting HQI>450 are defined as sweet spots.(3)Considering the characteristics of the organic matter conversion material field and seepage field,the temperature interval 300-370℃is recommended as the optimal heating window for the Chang 7_(3)sub-member of the Triassic Yanchang Formation in the Ordos Basin.Based on the advantages of thermal conductivity,permeability,and hydrocarbon expulsion efficiency along the bedding direction during in-situ heating,the“horizontal well heating+vertical well development”scheme is proposed,which has demonstrated significant enhancement in both recovery factor and energy output-input ratio,making it the optimal in-situ conversion process.The research findings provide a theoretical and technical foundation for the economical and efficient development of low-to-medium maturity shale oil.展开更多
In addition to the organic matter type,abundance,thermal maturity,and shale reservoir space,the preservation conditions of source rocks play a key factor in affecting the quantity and quality of retained hydrocarbons ...In addition to the organic matter type,abundance,thermal maturity,and shale reservoir space,the preservation conditions of source rocks play a key factor in affecting the quantity and quality of retained hydrocarbons in source rocks of lacustrine shale,yet this aspect has received little attention.This paper,based on the case analysis,explores how preservation conditions influence the enrichment of mobile hydrocarbons in shale oil.Research showns that good preservation conditions play three key roles.(1)Ensure the retention of sufficient light hydrocarbons(C_(1)–C_(13)),medium hydrocarbons(C_(14)–C_(25))and small molecular aromatics(including 1–2 benzene rings)in the formation,which enhances the fluidity and flow of shale oil;(2)Maintain a high energy field(abnormally high pressure),thus facilitating the maximum outflow of shale oil;(3)Ensure that the retained hydrocarbons have the miscible flow condition of multi-component hydrocarbons(light hydrocarbons,medium hydrocarbons,heavy hydrocarbons,and heteroatomic compounds),so that the heavy hydrocarbons(C_(25+))and heavy components(non-hydrocarbons and asphaltenes)have improved fluidity and maximum flow capacity.In conclusion,in addition to the advantages of organic matter type,abundance,thermal maturity,and reservoir space,good preservation conditions of shale layers are essential for the formation of economically viable shale oil reservoirs,which should be incorporated into the evaluation criteria of shale oil-rich areas/segments and considered a necessary factor when selecting favorable exploration targets.展开更多
Savannah,Ga.,May 20,2025-Gulfstream Aerospace Corp.today announced the 50th customer delivery of the Gulfstream G700.The aircraft was outfitted at Gulfstream's Savannah completions center.Since entering service in...Savannah,Ga.,May 20,2025-Gulfstream Aerospace Corp.today announced the 50th customer delivery of the Gulfstream G700.The aircraft was outfitted at Gulfstream's Savannah completions center.Since entering service in April 2024,the G700 operates globally,and the fleet has accumulated more than 11,700 flight hours.展开更多
The study aimed to develop a customized Data Governance Maturity Model (DGMM) for the Ministry of Defence (MoD) in Kenya to address data governance challenges in military settings. Current frameworks lack specific req...The study aimed to develop a customized Data Governance Maturity Model (DGMM) for the Ministry of Defence (MoD) in Kenya to address data governance challenges in military settings. Current frameworks lack specific requirements for the defence industry. The model uses Key Performance Indicators (KPIs) to enhance data governance procedures. Design Science Research guided the study, using qualitative and quantitative methods to gather data from MoD personnel. Major deficiencies were found in data integration, quality control, and adherence to data security regulations. The DGMM helps the MOD improve personnel, procedures, technology, and organizational elements related to data management. The model was tested against ISO/IEC 38500 and recommended for use in other government sectors with similar data governance issues. The DGMM has the potential to enhance data management efficiency, security, and compliance in the MOD and guide further research in military data governance.展开更多
This study explores the relationship between corporate environmental,social,and governance(ESG)disagreements and corporate debt maturity.By examining panel samples from Chinese non-financial listed companies covering ...This study explores the relationship between corporate environmental,social,and governance(ESG)disagreements and corporate debt maturity.By examining panel samples from Chinese non-financial listed companies covering 2007 to 2020,we find that ESG disagreements negatively influence corporate debt maturity.Even after conducting a series of robustness tests and addressing endogeneity concerns,the adverse effects of ESG disagreements persisted.A heterogeneity analysis shows that this negative impact is more significant for non-state-owned enterprises,small enterprises,enterprises with high capital intensity,enterprises with low analyst attention,and enterprises in high-tech industries.Through a mechanism analysis,we discovered that ESG disagreements can lead to information asymmetry and heightened default risk,subsequently affecting the maturity of corporate debt.Further analysis confirms that the negative impact of ESG on the debt structure inhibits long-term investment and exacerbates the mismatch between investment and financing terms.展开更多
Background Boars undergo physiological and biochemical changes in semen composition as they grow from puberty to sexual maturity.However,comprehensive metabolomic profiles of boar semen remain uncharacterised.Understa...Background Boars undergo physiological and biochemical changes in semen composition as they grow from puberty to sexual maturity.However,comprehensive metabolomic profiles of boar semen remain uncharacterised.Understanding metabolic alterations in semen during this period is important for optimising reproductive performance in breeding programs.The aim of this study was to characterise the semen metabolome as boars mature,utilising an untargeted metabolomic approach.Semen samples were collected from 15 Duroc boars at three developmental ages:~7 months,8.5 months,and 10 months.Sperm and seminal plasma were separated and analysed by hydrophilic interaction and reversed-phase liquid chromatography coupled with mass spectrometry to capture a wide range of metabolites.Results We identified a total of 4,491 features in boar semen,annotating 92 distinct metabolites.Amino acids,peptides and analogues constituted the most abundant components,followed by fatty acid esters.Principal component analysis(PCA)and partial least squares discriminant analysis(PLS-DA)showed a clear separation between metabolomic profiles by age groups.PERMANOVA analysis of PCA scores confirmed statistically significant differences(P<0.05)between younger(7 months)and more mature boars(8.5 months and 10 months).Pathway analysis identified porphyrin metabolism,taurine and hypotaurine metabolism,and glycerolipid metabolism as significantly enriched pathways in sperm,while glutathione and nitrogen metabolism were prominently enriched in seminal plasma.Using linear modelling,partial Spearman correlation and random forest analyses,we identified homoisovanillic acid as a key metabolite discriminating age groups in both sperm and seminal plasma.Additionally,L-glutamic acid,decanoyl-L-carnitine and N-(1,3-Thiazol-2-yl)benzenesulfonamide emerged as important sperm metabolites,while glyceric acid,myo-inositol,glycerophosphocholine,and several other compounds were identified as critical seminal plasma metabolites.Conclusion This study provides a detailed characterisation of metabolic changes in Duroc boar semen during the transition from puberty to sexual maturity.Our findings enhance the understanding of reproductive development and could inform strategies to assess sexual maturity in breeding programs.展开更多
Organic matter(OM)is the primary gas occurrence carrier in shale reservoirs due to their abundant nanopores.To reveal the OM pore structure,adsorption capacity and evolution during thermal maturation,this study collec...Organic matter(OM)is the primary gas occurrence carrier in shale reservoirs due to their abundant nanopores.To reveal the OM pore structure,adsorption capacity and evolution during thermal maturation,this study collected data from samples spanning the entire evolution stage,from immature to over-mature.Scanning Electron Microscope(SEM)observation and low temperature gases adsorption experiments were used to qualitatively-semi-quantitatively and quantitatively analyze OM pore structure evolution,and CH_(4) isothermal adsorption experiments were used to reveal the adsorption capacity evolution.Then,the influence and mechanism of matu rity and hydrocarbon generation on pore development and adsorption capacity were quantitatively reviewed based on the experimental data.The results show that OM pores are poorly developed in the immature stage due to weak hydrocarbon generation,although micro-fractures are occasionally found at the edges of OM particles.In the low maturity stage,OM pores are partially developed due to liquid hydrocarbon generation,with liquid hydrocarbons also filling some OM pores.The contribution of total organic carbon content(TOC)to adsorption extent is not significant in these two stages.From high to high-over maturity stages,massive gaseous hydrocarbons are generated,significantly improving the surface porosity of OM.Clear positive linear correlations are observed between TOC and adsorption amount.However,the development of OM pores significantly declines when thermal maturity(R_(o))exceeds 3.5%due to excessive aromatization.The accuracy of research on the evolution of pore structure and adsorption capacity is limited by several factors:(ⅰ)errors caused by sample specification,calculation processes,parameter settings,and kerogen models in isothermal adsorption experiments and molecular simulations;(ⅱ)difficulty in achieving control variables due to the strong heterogeneity of natural maturation shale samples;and(ⅲ)the need to enhance compatibility between thermal simulation experiments and natural thermal evolution.Therefore,isothermal adsorption experiments on bulk shale and molecular simulations of intact shale model are necessary,taking into account the dynamic temperature and pressure of in-situ reservoirs.Moreover,shale samples with varying maturity,influenced by their distance from the paleo-thermal source,may provide significant verification for thermal simulation experiments.展开更多
Light hydrocarbons(LHs)are key components of petroleum,and the carbon isotopes composition(δ^(13)C)of individual LHs contains a wealth of geochemical information.Forty-four oil samples from five different basins were...Light hydrocarbons(LHs)are key components of petroleum,and the carbon isotopes composition(δ^(13)C)of individual LHs contains a wealth of geochemical information.Forty-four oil samples from five different basins were analyzed using gas chromatography(GC),gas chromatography-mass spectrometry(GC–MS),and gas chromatography-isotope ratio mass spectrometry(GC-IRMS).Theδ^(13)C values of forty-three LHs were recognized and determined by comparing the GC and GC-IRMS methods.The results revealed significant differences inδ^(13)C distribution characteristics among different LH compounds.Theδ^(13)C variation of individual LHs in iso-paraffins showed the widest range,followed by cycloalkanes and aromatics,whereas theδ^(13)C variation in n-paraffins showed the narrowest range.Theδ^(13)C values of most individual LHs are primarily affected by the source facies and thermal evolution.Among them,c-1,3-dimethylcyclohexane(c-1,3DMCH)is mainly sourced from higher plants but may also form through abiotic mechanisms such as catalysis or cyclization.Theδ^(13)C values of c-1,3DMCH(δ^(13)Cc-1,3DMCH)primarily exhibit parental genetic characteristics,enabling effective distinction of oil from different source facies.Specifically,theδ^(13)Cc-1,3DMCH in marine oils,lacustrine oils,terrigenous oils,and coal-formed oils are<–22‰,from–22‰to−20.2‰,from−20.2‰to−18.4‰,and>−18.4‰,respectively.Moreover,maturity is the primary controlling factor forδ^(13)C values of 3MC7(δ^(13)C3MC7,3MC7:3-methylheptane),while the source facies serve as a secondary influence.The plot ofδ^(13)Cc-1,3DMCH andδ^(13)C3MC7 was introduced to classify source facies.Asδ^(13)Cc-1,3DMCH andδ^(13)C3MC7 increase,the source facies transits from marine to lacustrine,then terrigenous,and finally coal facies.Additionally,increasingδ^(13)C3MC7 indicates a relative increase in maturity.Therefore,theδ^(13)Cc-1,3DMCH vs.δ^(13)C3MC7 plot serves as an effective tool for distinguishing source facies and assessing relative maturity.展开更多
In the era of global tourism and creative economies,digital technology maturity has emerged as a critical factor for the sustainable development of small and medium enterprises(SMEs)in Indonesia.This research is imple...In the era of global tourism and creative economies,digital technology maturity has emerged as a critical factor for the sustainable development of small and medium enterprises(SMEs)in Indonesia.This research is implemented to find out current digital technology maturity levels among SMEs in the subdistrict of Cangkringan,district of Sleman,Yogyakarta,Indonesia,highlighting their role in fostering sustainable tourism and creative economic growth.Using a mixed-method approach,the research combines qualitative insights from in-depth interviews with three local government representatives and Focus Group Discussion with 15 SMEs in the area.Additionally,a quantitative analysis of SMEs across various sectors,including crafts and culinary services,supports the study’s conclusions.The finding reveals a significant gap between government’s training programs and the digital literacy of SMEs as well as a lack of affordable technological resources which could impede the full realization of digital benefits.Strengthening digital technology maturity among SMEs is essential for enhancing their competitiveness and long-term sustainability,yielding both financial and non-financial benefits.This also contributes to find out digital literacy gaps,impact on competitiveness and sustainability,policy and stakeholder recommendations,and government action.These insights offer valuable guidance for policymakers and stakeholders striving to support the digital transformation of SMEs in similar settings.The study also sheds light on digital literacy gaps,their impact on competitiveness and offers policy recommendations for government and stakeholders to address these challenges such as through government and community collaboration.展开更多
In this study, peaches of cv. ＇Sui Yuan＇ with different maturities were treated with 0.05 mM sodium nitroprusside （SNP）, a nitric oxide donor, for 5 min, at 28℃, and then stored at 0-1.5℃ for 30 days. Untreated ...In this study, peaches of cv. ＇Sui Yuan＇ with different maturities were treated with 0.05 mM sodium nitroprusside （SNP）, a nitric oxide donor, for 5 min, at 28℃, and then stored at 0-1.5℃ for 30 days. Untreated fruits stored under the same conditions were used as control fruits in the experiment. The browning level, activities of polyphenol oxidase （PPO）, peroxydase （POD） and superoxide dismutase （SOD）, and quality parameters （firmness, titratable acid, total soluble solids and total sugar） were analyzed. The results showed that the firmness of SNP-treated fruit at different stage of maturity were higher than that of the control fruit after storage for 30 days. However, no significant effect was found on total soluble solid content. The titratable acid content of low maturity fruit was higher in SNP-treated one than that of the control, but the titratable acid content of high maturity fruit treated by SNP was lower than that of the control on 10th and 30th day. In addition, the combination of SNP treatment and high maturity resulted in higher activities of SOD, lower activity of PPO and POD, lower browning level. Therefore, the combination of high maturity and SNP treatment might be a useful method to well maintain honey peach fruit quality during cold storage.展开更多
[Objective] The effects of ploughing Astragalus sinicus at maturity stage as green manure on paddy soil properties were studied to provide references for ad- vancing the green manure production and field sustainable d...[Objective] The effects of ploughing Astragalus sinicus at maturity stage as green manure on paddy soil properties were studied to provide references for ad- vancing the green manure production and field sustainable development. [Methods] A three-year field positioning experiment was carried out. In the first year, five differ- ent levels （0, 2.81, 5.61, 8.42, 11.23 t/hm~） of Astragalus sinicus ploughed at matu- rity stage were designed. And in the next two years, the amounts of ploughed As- tragalus sinicus at maturity stage were based on the natural growth amounts of As- tragalus sinicus at maturity stage of the former year in each plot field. The yields of Astragalus sinicus at maturity stage were estimated and ploughed directly before rice transplanting. Rice yield, soil nutrients, biological properties and water-stable aggre- gates of paddy fields were measured as well. [Results] The effective panicles and rice yields increased significantly after ploughing Astragalus sinicus at maturity stage. Meanwhile, the organic acid content in paddy field increased and the activities of soil phosphatases, invertase and catalase were higher. The soil dissolved organic carbon also increased with the increasing application of green manure. However, excess amount of green manure inhibited the soil microbial biomass carbon content. The macro aggregates and micro aggregates gathered to middle aggregates. [Conclusion] As green manure, the use of Astragalus sinicus at maturity stage could improve soil physiochemical and biological properties, improve soil fertility and increase rice yield significantly. But the ploughing amount of Astragalus sinicus at maturity stage before rice transplanting should be less than 8.42 t/hm2 （dry weight.展开更多
Based on the concept of debt duration,this paper proposes the elasticity of cash flow.Then,the debt maturity structure in project financing is discussed.The results show that in the project financing structure,the deb...Based on the concept of debt duration,this paper proposes the elasticity of cash flow.Then,the debt maturity structure in project financing is discussed.The results show that in the project financing structure,the debt maturity structure is closely related with debt capacity.Higher debt ratio requires short term debt,and vise versa.展开更多
In the Jimusaer Sag of the Junggar Basin,crude oils from the upper and lower sweet-spot intervals of the Permian Lucaogou Formation display a pronounced“light-heavy reversal”in oil properties that indicates a fundam...In the Jimusaer Sag of the Junggar Basin,crude oils from the upper and lower sweet-spot intervals of the Permian Lucaogou Formation display a pronounced“light-heavy reversal”in oil properties that indicates a fundamental mismatch between oil composition and host rock maturity.To resolve this anomaly,this study integrates geological,geochemical,and petrophysical datasets and systematically evaluates the combined roles of thermal evolution,organofacies,wettability,abnormal overpressure,and migration-related fractionation on shale oil composition.On this basis,a“staged charging-cumulative charging”model is proposed to explain compositional heterogeneity in lacustrine shale oils.The results demonstrate that crude-oil compositions are jointly controlled by the extent of biomarker depletion,the temporal evolution of hydrocarbon charging,and the openness of the source-reservoir system,rather than by thermal maturity or organofacies alone.The upper sweet-spot interval is interpreted to have functioned as a semi-open system during early stages,in which hydrocarbon generation and expulsion were broadly synchronous,leading to preferential loss of early-generated,biomarker-rich heavy components,whereas progressive shale diagenesis at later stages promoted the retention of highly mature,light hydrocarbons.In contrast,the lower sweet-spot interval represents a relatively closed system,where hydrocarbons generated during multiple stages continuously accumulated and were preserved as mixed charges;overprinting by multi-phase fluids progressively weakened sterane isomerization signals,rendering them unreliable indicators of individual charging events or final thermal maturity.This charging behavior provides a reasonable explanation for anomalously low or distorted biomarker parameters observed in intervals of low or similar maturity.Overall,the proposed charging model reconciles the observed reversal in crude-oil properties and,by shifting the interpretive focus from static maturity assessment to charging dynamics,offers a new theoretical basis for understanding lacustrine shale oil accumulation processes,and guiding sweet-spot selection and exploration-development strategies.展开更多
The lesser spiny eel(Macrognathus aculeatus)is a significant species in Southeast Asia’s freshwater ecosystems,particularly in the Progo River,Indonesia.This study investigates the reproductive biology of the lesser ...The lesser spiny eel(Macrognathus aculeatus)is a significant species in Southeast Asia’s freshwater ecosystems,particularly in the Progo River,Indonesia.This study investigates the reproductive biology of the lesser spiny eel,focusing on size,weight,sex ratio,gonad maturity,fertility,and environmental influences on spawning.A total of 217 eels were sampled,and data on gonadal maturity index(GMI),fecundity,and oocyte size were collected and analyzed.The study found a balanced sex ratio of 0.95:1.0,with significant fluctuations during the spawning season.Female eels averaged 33.4 cm in length and 130.4 g in weight,while males averaged 31.6 cm and 107.7 g.Over 58%of females reached gonadal maturity level IV during peak spawning months,indicating synchronized reproductive cycles.The average fecundity at maturity level IV was 2062±605 grains,with larger oocytes being released during spawning.The findings highlight the importance of hydrological conditions in the reproductive success of lesser spiny eels.The study confirms that environmental factors,such as rainfall and water temperature,significantly influence spawning behavior.The low relative fecundity suggests vulnerability to overfishing and habitat degradation,emphasizing the need for targeted conservation efforts.The lesser spiny eel’s reproductive biology is intricately linked to environmental conditions,necessitating comprehensive conservation strategies that include habitat protection and community engagement.By fostering local involvement and awareness,conservation initiatives can enhance the sustainability of this species and its ecosystem,ensuring the long-term viability of the lesser spiny eel in the Progo River.展开更多
Marine pollution and overfishing induced the biodiversity loss and ecological degradation of the Beibu Gulf ecosystem in Guangxi,SE China.In an effort to restore the ecosystem and fishery resources,artificial reefs we...Marine pollution and overfishing induced the biodiversity loss and ecological degradation of the Beibu Gulf ecosystem in Guangxi,SE China.In an effort to restore the ecosystem and fishery resources,artificial reefs were deployed in the Beibu Gulf as the marine ranching area and their ecological performance need to be investigated.We constructed Ecopath ecological trophic models for the marine ranching area and a nearby control area to compare their ecosystem throughput and food web structure difference,and to calculate the ecological carrying capacity of various functional groups.Results indicate that the total system throughput of the marine ranching area was significantly higher than the control area,and the majority of system throughput occurred at trophic levelsⅠandⅡin both ecosystems.The system connectance indices for the marine ranching and control areas were 0.27 and 0.32,and the omnivory indices were 0.16 and 0.19,indicating simple food web structures;both areas are in a developmental stage with TPP/TR ratios of 2.69 and 9.36,respectively.Compared to the control area,marine ranching area exhibited a higher system maturity,and the ecological carrying capacity of“large and medium-sized demersal fish”and“other bivalves”functional groups in the marine ranching area increased by 43.83%and 233.62%,respectively,allowing for more high-trophic-level predators and large benthic animals.This study provided a reference for the formulation of fishery management policies in the Beibu Gulf,to maintain ecosystem stability and biodiversity.展开更多
Flowering time is a critical agronomic trait with a profound effect on the productivity and adaptabillity of rapeseed(Brassica napus L.).Strategically advancing flowering time can reduce the risk of yield losses due t...Flowering time is a critical agronomic trait with a profound effect on the productivity and adaptabillity of rapeseed(Brassica napus L.).Strategically advancing flowering time can reduce the risk of yield losses due to extreme climatic conditions and facilitate the cultivation of subsequent crops on the same land,thereby enhancing overall agricultural efficiency.In this review,we synthesize current information on flowering time regulation in rapeseed through an integrated analysis of its genetic,hormonal,and environmental dimensions,emphasizing their crosstalk and implications for yield.We consolidate multi-omics evidence from population genetics,functional genomics,and systems biology to create a haplotype-based framework that overcomes the trade-off between flowering time and yield,providing support for the precision breeding of early-maturing cultivars.The insights presented here could inform future research on flowering time regulation and guide strategies for increasing rapeseed productivity.展开更多
Chili fermentation is one of the most important processes in the production of Pixian Douban(PXDB),which determines the flavor and product quality of PXDB.However,the maturity of fermented chili mainly depends on empi...Chili fermentation is one of the most important processes in the production of Pixian Douban(PXDB),which determines the flavor and product quality of PXDB.However,the maturity of fermented chili mainly depends on empirical judgment,which can not satisfy the need for standardized production of PXDB.Therefore,this study aimed to investigate volatile and non-volatile substances during the maturation process and to find markers related to the maturity of fermented chili.Two-dimensional gas chromatography-mass spectrometry(GC×GC-MS)combined with multivariate statistical analysis and relative odor activity values(ROAV)analysis revealed that 2-methoxy-3-isobutyl pyrazine,linalool,3-(methylthio)propionaldehyde,myrcene,and decanal(ROAV≥1,VIP>1,P<0.05)were regarded as potentially active aromatic markers for differentiating fermentation time.Additionally,ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLCQTOF-MS)combined with multivariate statistical analysis revealed that 25 compounds could serve as differentiated non-volatile compounds.The correlation of maturity-related physicochemical indicators with volatile and nonvolatile compounds revealed that four volatile compounds(2-methoxy-3-isobutylpyrazine,linalool,myrcene,and decanal)along with seven non-volatile compounds could serve as markers for evaluating the maturity of fermented chili.This study is expected to establish a standard for the determination of the maturity the fermented chili and lay the foundation for intelligent production of PXDB.展开更多
Objectives:B-cell maturation antigen(BCMA)-targeted antibody–drug conjugates(ADCs)have emerged as promising therapies for relapsed/refractory multiple myeloma(RRMM),but the overall efficacy and safety profile is uncl...Objectives:B-cell maturation antigen(BCMA)-targeted antibody–drug conjugates(ADCs)have emerged as promising therapies for relapsed/refractory multiple myeloma(RRMM),but the overall efficacy and safety profile is unclear.This study aimed to synthesize the available evidence on the safety and efficacy of BCMA-ADCs in development for RRMM.Methods:A systematic search was conducted using six bibliographic databases and ClinicalTrials.gov up to November 2024.Studies were eligible if they were human clinical trials or animal studies evaluating BCMA-ADCs and reported efficacy and safety outcomes.Data extraction and quality assessments were conducted using validated tools,including ROBINS-I and SYRCLE’s risk of bias tool.Results:A total of 21 studies were included:16 clinical trials and five animal studies.Key findings included that belantamab mafodotin demonstrated variable but generally durable response rates(32%–85%)and a broad range of progression-free survival(PFS)(2.8–36.6 months),albeit with ocular toxicities in 51%–96%.Among newer candidates,MEDI2228 showed median PFS 5.1–6.6 months with 14%discontinuation for ocular symptoms,while AMG 224 had an overall response rate(ORR)of 23%(9/40)with anemia 21%,thrombocytopenia 24%,and ocular adverse events(AEs)21%.Animal studies supported the tumor-eradicating potential of all BCMA-ADC candidates,although safety signals such as hepatic and renal toxicity were noted with HDP-101.The risk of bias assessment revealed generally moderate to serious concerns in human trials,while the overall quality of the animal studies was acceptable.Conclusions:BCMA-targeted ADC candidates show encouraging efficacy in RRMM,particularly belantamab mafodotin.However,frequent AEs,especially ocular and hematologic toxicities,underscore the need for optimization in ADC design.Further research should prioritize enhancing safety while maintaining clinical benefit.展开更多
In vitro maturation(IvM)of human oocytes offers cost efficiency and minimal invasiveness,serving as a valuable supplementary tool in assisted reproduction for fertility preservation,ovarian hyperstimulation syndrome p...In vitro maturation(IvM)of human oocytes offers cost efficiency and minimal invasiveness,serving as a valuable supplementary tool in assisted reproduction for fertility preservation,ovarian hyperstimulation syndrome prevention,and other reproductive strategies.Despite its availability for three decades,the clinical use of IVM remains limited due to efficacy and safety concerns.This study examines the DNA methylation profile of IVM oocytes collected during laparoscopic/hysteroscopic surgeries compared to in vivo matured oocytes via reduced representation bisulfite sequencing.Results indicate IVM oocytes exhibit a higher global methylation level.Differentially methylated regions(DMRs)analysis reveals that the in vitro group displays more hypermethylated and fewer hypomethylated DMRs compared to the in vivo group.Additionally,the in vitro group exhibits a higher level of non-CpG methylation than the in vivo group.However,no significant correlation between methylation levels and transcriptional activity in these oocytes is found,especially for those specific imprinted genes or genes related to embryonic development.These findings shed light on the epigenetic landscape of IvM oocytes,contributing to the ongoing assessment of their clinical feasibility and safety in assisted reproduction.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.32171997)the Earmarked Fund for China Agricultural Research System(CARS-13)+2 种基金the Nanfan special project of CAAS(Grant No.YBXM2552)the Central Public-interest Scientific Institution Basal Research Fund(Grant No.Y2025YC112)the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(Grant No.CAAS-ASTIP-2021-OCRI)。
文摘Peanut(Arachis hypogaea L.)exhibits an unusually asynchronous reproductive cycle,in which flowering,peg penetration,pod development,and seed filling occur over an extended period.This results in the simultaneous presence of immature and preharvest sprouted(PHS)pods on the same plant a dual challenge that undermines yield,compromises seed quality,and complicates postharvest management.Immature pods reduce harvest efficiency,while PHS diminishes flavor,uniformity,and storage stability.Both genetic and environmental determinants ranging from temporal variation in peg initiation and hormonal gradients to microenvironmental heterogeneity and differential seed dormancy shape this variability.However,despite advances in pod biology,systematic field-based quantification of intra-plant temporal variation,genotype×environment interactions,and localized microclimatic influences remains limited.This review aims to synthesize current understanding of within-plant variability in pod maturation and PHS in peanut,to elucidate critical knowledge gaps at physiological and field scales,and to evaluate emerging strategies for mitigation.Particular emphasis is given for underexplored interface between physiological mechanisms and field-scale dynamics.Emerging innovations including hyperspectral imaging,soil and canopy moisture sensing,and molecular markers offer promising avenues for precise monitoring of pod maturity and early detection of PHS risk.Integrating these tools with targeted breeding strategies for synchronous flowering,enhanced dormancy,and late-season stress resilience,alongside adaptive agronomic practices such as optimized sowing,irrigation scheduling,nutrient management,and harvest timing,could substantially reduce yield and quality losses.Future progress will depend on bridging molecular insights with predictive models that capture mixed maturity and sprouting risk under variable environments.
基金Supported by the National Natural Science Foundation of China Enterprise Innovation and Development Joint Fund Project(U22B6004)National Natural Science Foundation of China and Youth Science Fund Project(4250021468)CNPC Changqing Oilfield Company Key Core Technology Research Project(KJZX2023-01)。
文摘In-situ heating conversion is the most practical recovery method for lacustrine low-to-medium maturity shale oil.However,the energy output-input ratio must exceed the economic threshold to achieve commercial development.This paper systematically investigates the mechanism of super-rich accumulation of organic matter in continental shale,sweet spot evaluation,optimal heating windows,and appropriate well types and patterns from the perspectives of enhancing energy output and reducing energy input.(1)The super-rich accumulation of organic matter in lacustrine shale is primarily controlled by the intensity,frequency,and preservation of external material inputs,and is related to moderate volcanic and hydrothermal activities,marine transgressions,with total organic carbon content greater than or equal to 6%.(2)The quality of organic-rich intervals is related to the type of source material and hydrocarbon generation potential.The in-situ conversion-derived hydrocarbon quality index(HQI)is established,and the zones exhibiting HQI>450 are defined as sweet spots.(3)Considering the characteristics of the organic matter conversion material field and seepage field,the temperature interval 300-370℃is recommended as the optimal heating window for the Chang 7_(3)sub-member of the Triassic Yanchang Formation in the Ordos Basin.Based on the advantages of thermal conductivity,permeability,and hydrocarbon expulsion efficiency along the bedding direction during in-situ heating,the“horizontal well heating+vertical well development”scheme is proposed,which has demonstrated significant enhancement in both recovery factor and energy output-input ratio,making it the optimal in-situ conversion process.The research findings provide a theoretical and technical foundation for the economical and efficient development of low-to-medium maturity shale oil.
基金Supported by the National Natural Science Foundation of China(U22B6004)Project of PetroChina Research Institute of Petroleum Exploration and Development(2022yjcq03)Core Technology Key Project of China Petroleum Changqing Oilfield Company(KJZX2023-01).
文摘In addition to the organic matter type,abundance,thermal maturity,and shale reservoir space,the preservation conditions of source rocks play a key factor in affecting the quantity and quality of retained hydrocarbons in source rocks of lacustrine shale,yet this aspect has received little attention.This paper,based on the case analysis,explores how preservation conditions influence the enrichment of mobile hydrocarbons in shale oil.Research showns that good preservation conditions play three key roles.(1)Ensure the retention of sufficient light hydrocarbons(C_(1)–C_(13)),medium hydrocarbons(C_(14)–C_(25))and small molecular aromatics(including 1–2 benzene rings)in the formation,which enhances the fluidity and flow of shale oil;(2)Maintain a high energy field(abnormally high pressure),thus facilitating the maximum outflow of shale oil;(3)Ensure that the retained hydrocarbons have the miscible flow condition of multi-component hydrocarbons(light hydrocarbons,medium hydrocarbons,heavy hydrocarbons,and heteroatomic compounds),so that the heavy hydrocarbons(C_(25+))and heavy components(non-hydrocarbons and asphaltenes)have improved fluidity and maximum flow capacity.In conclusion,in addition to the advantages of organic matter type,abundance,thermal maturity,and reservoir space,good preservation conditions of shale layers are essential for the formation of economically viable shale oil reservoirs,which should be incorporated into the evaluation criteria of shale oil-rich areas/segments and considered a necessary factor when selecting favorable exploration targets.
文摘Savannah,Ga.,May 20,2025-Gulfstream Aerospace Corp.today announced the 50th customer delivery of the Gulfstream G700.The aircraft was outfitted at Gulfstream's Savannah completions center.Since entering service in April 2024,the G700 operates globally,and the fleet has accumulated more than 11,700 flight hours.
文摘The study aimed to develop a customized Data Governance Maturity Model (DGMM) for the Ministry of Defence (MoD) in Kenya to address data governance challenges in military settings. Current frameworks lack specific requirements for the defence industry. The model uses Key Performance Indicators (KPIs) to enhance data governance procedures. Design Science Research guided the study, using qualitative and quantitative methods to gather data from MoD personnel. Major deficiencies were found in data integration, quality control, and adherence to data security regulations. The DGMM helps the MOD improve personnel, procedures, technology, and organizational elements related to data management. The model was tested against ISO/IEC 38500 and recommended for use in other government sectors with similar data governance issues. The DGMM has the potential to enhance data management efficiency, security, and compliance in the MOD and guide further research in military data governance.
基金STU Scientific Research Initiation Grant[Grant No.STF24004T]Natural Science Foundation of Guangdong Province of China[Grant No.2021B1515020103].
文摘This study explores the relationship between corporate environmental,social,and governance(ESG)disagreements and corporate debt maturity.By examining panel samples from Chinese non-financial listed companies covering 2007 to 2020,we find that ESG disagreements negatively influence corporate debt maturity.Even after conducting a series of robustness tests and addressing endogeneity concerns,the adverse effects of ESG disagreements persisted.A heterogeneity analysis shows that this negative impact is more significant for non-state-owned enterprises,small enterprises,enterprises with high capital intensity,enterprises with low analyst attention,and enterprises in high-tech industries.Through a mechanism analysis,we discovered that ESG disagreements can lead to information asymmetry and heightened default risk,subsequently affecting the maturity of corporate debt.Further analysis confirms that the negative impact of ESG on the debt structure inhibits long-term investment and exacerbates the mismatch between investment and financing terms.
基金Open access funding provided by University of Inland Norway The Research Council of Norway provided financial support(grant number 331878).
文摘Background Boars undergo physiological and biochemical changes in semen composition as they grow from puberty to sexual maturity.However,comprehensive metabolomic profiles of boar semen remain uncharacterised.Understanding metabolic alterations in semen during this period is important for optimising reproductive performance in breeding programs.The aim of this study was to characterise the semen metabolome as boars mature,utilising an untargeted metabolomic approach.Semen samples were collected from 15 Duroc boars at three developmental ages:~7 months,8.5 months,and 10 months.Sperm and seminal plasma were separated and analysed by hydrophilic interaction and reversed-phase liquid chromatography coupled with mass spectrometry to capture a wide range of metabolites.Results We identified a total of 4,491 features in boar semen,annotating 92 distinct metabolites.Amino acids,peptides and analogues constituted the most abundant components,followed by fatty acid esters.Principal component analysis(PCA)and partial least squares discriminant analysis(PLS-DA)showed a clear separation between metabolomic profiles by age groups.PERMANOVA analysis of PCA scores confirmed statistically significant differences(P<0.05)between younger(7 months)and more mature boars(8.5 months and 10 months).Pathway analysis identified porphyrin metabolism,taurine and hypotaurine metabolism,and glycerolipid metabolism as significantly enriched pathways in sperm,while glutathione and nitrogen metabolism were prominently enriched in seminal plasma.Using linear modelling,partial Spearman correlation and random forest analyses,we identified homoisovanillic acid as a key metabolite discriminating age groups in both sperm and seminal plasma.Additionally,L-glutamic acid,decanoyl-L-carnitine and N-(1,3-Thiazol-2-yl)benzenesulfonamide emerged as important sperm metabolites,while glyceric acid,myo-inositol,glycerophosphocholine,and several other compounds were identified as critical seminal plasma metabolites.Conclusion This study provides a detailed characterisation of metabolic changes in Duroc boar semen during the transition from puberty to sexual maturity.Our findings enhance the understanding of reproductive development and could inform strategies to assess sexual maturity in breeding programs.
基金the supports of the Na-tional Natural Science Foundation of China(No.U19B2007,42072202)the China Postdoctoral Science Foundation(No.2025MD774057)。
文摘Organic matter(OM)is the primary gas occurrence carrier in shale reservoirs due to their abundant nanopores.To reveal the OM pore structure,adsorption capacity and evolution during thermal maturation,this study collected data from samples spanning the entire evolution stage,from immature to over-mature.Scanning Electron Microscope(SEM)observation and low temperature gases adsorption experiments were used to qualitatively-semi-quantitatively and quantitatively analyze OM pore structure evolution,and CH_(4) isothermal adsorption experiments were used to reveal the adsorption capacity evolution.Then,the influence and mechanism of matu rity and hydrocarbon generation on pore development and adsorption capacity were quantitatively reviewed based on the experimental data.The results show that OM pores are poorly developed in the immature stage due to weak hydrocarbon generation,although micro-fractures are occasionally found at the edges of OM particles.In the low maturity stage,OM pores are partially developed due to liquid hydrocarbon generation,with liquid hydrocarbons also filling some OM pores.The contribution of total organic carbon content(TOC)to adsorption extent is not significant in these two stages.From high to high-over maturity stages,massive gaseous hydrocarbons are generated,significantly improving the surface porosity of OM.Clear positive linear correlations are observed between TOC and adsorption amount.However,the development of OM pores significantly declines when thermal maturity(R_(o))exceeds 3.5%due to excessive aromatization.The accuracy of research on the evolution of pore structure and adsorption capacity is limited by several factors:(ⅰ)errors caused by sample specification,calculation processes,parameter settings,and kerogen models in isothermal adsorption experiments and molecular simulations;(ⅱ)difficulty in achieving control variables due to the strong heterogeneity of natural maturation shale samples;and(ⅲ)the need to enhance compatibility between thermal simulation experiments and natural thermal evolution.Therefore,isothermal adsorption experiments on bulk shale and molecular simulations of intact shale model are necessary,taking into account the dynamic temperature and pressure of in-situ reservoirs.Moreover,shale samples with varying maturity,influenced by their distance from the paleo-thermal source,may provide significant verification for thermal simulation experiments.
基金funded by the National Natural Science Foundation of China(Grant No.42173054).
文摘Light hydrocarbons(LHs)are key components of petroleum,and the carbon isotopes composition(δ^(13)C)of individual LHs contains a wealth of geochemical information.Forty-four oil samples from five different basins were analyzed using gas chromatography(GC),gas chromatography-mass spectrometry(GC–MS),and gas chromatography-isotope ratio mass spectrometry(GC-IRMS).Theδ^(13)C values of forty-three LHs were recognized and determined by comparing the GC and GC-IRMS methods.The results revealed significant differences inδ^(13)C distribution characteristics among different LH compounds.Theδ^(13)C variation of individual LHs in iso-paraffins showed the widest range,followed by cycloalkanes and aromatics,whereas theδ^(13)C variation in n-paraffins showed the narrowest range.Theδ^(13)C values of most individual LHs are primarily affected by the source facies and thermal evolution.Among them,c-1,3-dimethylcyclohexane(c-1,3DMCH)is mainly sourced from higher plants but may also form through abiotic mechanisms such as catalysis or cyclization.Theδ^(13)C values of c-1,3DMCH(δ^(13)Cc-1,3DMCH)primarily exhibit parental genetic characteristics,enabling effective distinction of oil from different source facies.Specifically,theδ^(13)Cc-1,3DMCH in marine oils,lacustrine oils,terrigenous oils,and coal-formed oils are<–22‰,from–22‰to−20.2‰,from−20.2‰to−18.4‰,and>−18.4‰,respectively.Moreover,maturity is the primary controlling factor forδ^(13)C values of 3MC7(δ^(13)C3MC7,3MC7:3-methylheptane),while the source facies serve as a secondary influence.The plot ofδ^(13)Cc-1,3DMCH andδ^(13)C3MC7 was introduced to classify source facies.Asδ^(13)Cc-1,3DMCH andδ^(13)C3MC7 increase,the source facies transits from marine to lacustrine,then terrigenous,and finally coal facies.Additionally,increasingδ^(13)C3MC7 indicates a relative increase in maturity.Therefore,theδ^(13)Cc-1,3DMCH vs.δ^(13)C3MC7 plot serves as an effective tool for distinguishing source facies and assessing relative maturity.
文摘In the era of global tourism and creative economies,digital technology maturity has emerged as a critical factor for the sustainable development of small and medium enterprises(SMEs)in Indonesia.This research is implemented to find out current digital technology maturity levels among SMEs in the subdistrict of Cangkringan,district of Sleman,Yogyakarta,Indonesia,highlighting their role in fostering sustainable tourism and creative economic growth.Using a mixed-method approach,the research combines qualitative insights from in-depth interviews with three local government representatives and Focus Group Discussion with 15 SMEs in the area.Additionally,a quantitative analysis of SMEs across various sectors,including crafts and culinary services,supports the study’s conclusions.The finding reveals a significant gap between government’s training programs and the digital literacy of SMEs as well as a lack of affordable technological resources which could impede the full realization of digital benefits.Strengthening digital technology maturity among SMEs is essential for enhancing their competitiveness and long-term sustainability,yielding both financial and non-financial benefits.This also contributes to find out digital literacy gaps,impact on competitiveness and sustainability,policy and stakeholder recommendations,and government action.These insights offer valuable guidance for policymakers and stakeholders striving to support the digital transformation of SMEs in similar settings.The study also sheds light on digital literacy gaps,their impact on competitiveness and offers policy recommendations for government and stakeholders to address these challenges such as through government and community collaboration.
基金supported by National Key Technology R&D Program in the 11~(th) Five year Plan of China (2006BAD22B04)
文摘In this study, peaches of cv. ＇Sui Yuan＇ with different maturities were treated with 0.05 mM sodium nitroprusside （SNP）, a nitric oxide donor, for 5 min, at 28℃, and then stored at 0-1.5℃ for 30 days. Untreated fruits stored under the same conditions were used as control fruits in the experiment. The browning level, activities of polyphenol oxidase （PPO）, peroxydase （POD） and superoxide dismutase （SOD）, and quality parameters （firmness, titratable acid, total soluble solids and total sugar） were analyzed. The results showed that the firmness of SNP-treated fruit at different stage of maturity were higher than that of the control fruit after storage for 30 days. However, no significant effect was found on total soluble solid content. The titratable acid content of low maturity fruit was higher in SNP-treated one than that of the control, but the titratable acid content of high maturity fruit treated by SNP was lower than that of the control on 10th and 30th day. In addition, the combination of SNP treatment and high maturity resulted in higher activities of SOD, lower activity of PPO and POD, lower browning level. Therefore, the combination of high maturity and SNP treatment might be a useful method to well maintain honey peach fruit quality during cold storage.
基金Supported by the Important Science and Technology Program for Agriculture of Zhejiang Province(2009C2001-TZ)the Taizhou Research and Development of Applied Technology,Zhejiang Province(091TG06)~~
文摘[Objective] The effects of ploughing Astragalus sinicus at maturity stage as green manure on paddy soil properties were studied to provide references for ad- vancing the green manure production and field sustainable development. [Methods] A three-year field positioning experiment was carried out. In the first year, five differ- ent levels （0, 2.81, 5.61, 8.42, 11.23 t/hm~） of Astragalus sinicus ploughed at matu- rity stage were designed. And in the next two years, the amounts of ploughed As- tragalus sinicus at maturity stage were based on the natural growth amounts of As- tragalus sinicus at maturity stage of the former year in each plot field. The yields of Astragalus sinicus at maturity stage were estimated and ploughed directly before rice transplanting. Rice yield, soil nutrients, biological properties and water-stable aggre- gates of paddy fields were measured as well. [Results] The effective panicles and rice yields increased significantly after ploughing Astragalus sinicus at maturity stage. Meanwhile, the organic acid content in paddy field increased and the activities of soil phosphatases, invertase and catalase were higher. The soil dissolved organic carbon also increased with the increasing application of green manure. However, excess amount of green manure inhibited the soil microbial biomass carbon content. The macro aggregates and micro aggregates gathered to middle aggregates. [Conclusion] As green manure, the use of Astragalus sinicus at maturity stage could improve soil physiochemical and biological properties, improve soil fertility and increase rice yield significantly. But the ploughing amount of Astragalus sinicus at maturity stage before rice transplanting should be less than 8.42 t/hm2 （dry weight.
文摘Based on the concept of debt duration,this paper proposes the elasticity of cash flow.Then,the debt maturity structure in project financing is discussed.The results show that in the project financing structure,the debt maturity structure is closely related with debt capacity.Higher debt ratio requires short term debt,and vise versa.
基金Supported by the National Natural Science Foundation of China(42173030,42302161,42473034)State Science and Technology Major Project for New Oil and Gas Exploration and Development,Ministry of Science and Technology(2025ZD1400803)。
文摘In the Jimusaer Sag of the Junggar Basin,crude oils from the upper and lower sweet-spot intervals of the Permian Lucaogou Formation display a pronounced“light-heavy reversal”in oil properties that indicates a fundamental mismatch between oil composition and host rock maturity.To resolve this anomaly,this study integrates geological,geochemical,and petrophysical datasets and systematically evaluates the combined roles of thermal evolution,organofacies,wettability,abnormal overpressure,and migration-related fractionation on shale oil composition.On this basis,a“staged charging-cumulative charging”model is proposed to explain compositional heterogeneity in lacustrine shale oils.The results demonstrate that crude-oil compositions are jointly controlled by the extent of biomarker depletion,the temporal evolution of hydrocarbon charging,and the openness of the source-reservoir system,rather than by thermal maturity or organofacies alone.The upper sweet-spot interval is interpreted to have functioned as a semi-open system during early stages,in which hydrocarbon generation and expulsion were broadly synchronous,leading to preferential loss of early-generated,biomarker-rich heavy components,whereas progressive shale diagenesis at later stages promoted the retention of highly mature,light hydrocarbons.In contrast,the lower sweet-spot interval represents a relatively closed system,where hydrocarbons generated during multiple stages continuously accumulated and were preserved as mixed charges;overprinting by multi-phase fluids progressively weakened sterane isomerization signals,rendering them unreliable indicators of individual charging events or final thermal maturity.This charging behavior provides a reasonable explanation for anomalously low or distorted biomarker parameters observed in intervals of low or similar maturity.Overall,the proposed charging model reconciles the observed reversal in crude-oil properties and,by shifting the interpretive focus from static maturity assessment to charging dynamics,offers a new theoretical basis for understanding lacustrine shale oil accumulation processes,and guiding sweet-spot selection and exploration-development strategies.
文摘The lesser spiny eel(Macrognathus aculeatus)is a significant species in Southeast Asia’s freshwater ecosystems,particularly in the Progo River,Indonesia.This study investigates the reproductive biology of the lesser spiny eel,focusing on size,weight,sex ratio,gonad maturity,fertility,and environmental influences on spawning.A total of 217 eels were sampled,and data on gonadal maturity index(GMI),fecundity,and oocyte size were collected and analyzed.The study found a balanced sex ratio of 0.95:1.0,with significant fluctuations during the spawning season.Female eels averaged 33.4 cm in length and 130.4 g in weight,while males averaged 31.6 cm and 107.7 g.Over 58%of females reached gonadal maturity level IV during peak spawning months,indicating synchronized reproductive cycles.The average fecundity at maturity level IV was 2062±605 grains,with larger oocytes being released during spawning.The findings highlight the importance of hydrological conditions in the reproductive success of lesser spiny eels.The study confirms that environmental factors,such as rainfall and water temperature,significantly influence spawning behavior.The low relative fecundity suggests vulnerability to overfishing and habitat degradation,emphasizing the need for targeted conservation efforts.The lesser spiny eel’s reproductive biology is intricately linked to environmental conditions,necessitating comprehensive conservation strategies that include habitat protection and community engagement.By fostering local involvement and awareness,conservation initiatives can enhance the sustainability of this species and its ecosystem,ensuring the long-term viability of the lesser spiny eel in the Progo River.
基金Supported by the National Key R&D Program of China(No.2022YFD2401301)the Primary Research and Development Plan of Guangxi Province(No.GuiKe AB21220064)+1 种基金the National Natural Science Foundation of China(Nos.42106102,42306151)the Shandong Postdoctoral Science Foundation(No.SDCXZG202301009)。
文摘Marine pollution and overfishing induced the biodiversity loss and ecological degradation of the Beibu Gulf ecosystem in Guangxi,SE China.In an effort to restore the ecosystem and fishery resources,artificial reefs were deployed in the Beibu Gulf as the marine ranching area and their ecological performance need to be investigated.We constructed Ecopath ecological trophic models for the marine ranching area and a nearby control area to compare their ecosystem throughput and food web structure difference,and to calculate the ecological carrying capacity of various functional groups.Results indicate that the total system throughput of the marine ranching area was significantly higher than the control area,and the majority of system throughput occurred at trophic levelsⅠandⅡin both ecosystems.The system connectance indices for the marine ranching and control areas were 0.27 and 0.32,and the omnivory indices were 0.16 and 0.19,indicating simple food web structures;both areas are in a developmental stage with TPP/TR ratios of 2.69 and 9.36,respectively.Compared to the control area,marine ranching area exhibited a higher system maturity,and the ecological carrying capacity of“large and medium-sized demersal fish”and“other bivalves”functional groups in the marine ranching area increased by 43.83%and 233.62%,respectively,allowing for more high-trophic-level predators and large benthic animals.This study provided a reference for the formulation of fishery management policies in the Beibu Gulf,to maintain ecosystem stability and biodiversity.
基金supported by the National Key Research and Development Program of China(2022YFD1200400)the National Natural Science Foundation of China(32272111)+4 种基金Special fund for youth team of the Southwest Universities(SWU-XJPY202306)Chongqing Natural Science Foundation(CSTB2024NSCQLZX0012)Modern Agro-industry Technology Research System(CARS-12)Chongqing Modern Agricultural Industry Technology System(COMAITS202504)Biological Breeding-National Science and Technology Major Project(2022ZD04008).We sincerely appreciate the Plant Editors team for English language editing of the manuscript,which significantly improved its clarity and overall quality.
文摘Flowering time is a critical agronomic trait with a profound effect on the productivity and adaptabillity of rapeseed(Brassica napus L.).Strategically advancing flowering time can reduce the risk of yield losses due to extreme climatic conditions and facilitate the cultivation of subsequent crops on the same land,thereby enhancing overall agricultural efficiency.In this review,we synthesize current information on flowering time regulation in rapeseed through an integrated analysis of its genetic,hormonal,and environmental dimensions,emphasizing their crosstalk and implications for yield.We consolidate multi-omics evidence from population genetics,functional genomics,and systems biology to create a haplotype-based framework that overcomes the trade-off between flowering time and yield,providing support for the precision breeding of early-maturing cultivars.The insights presented here could inform future research on flowering time regulation and guide strategies for increasing rapeseed productivity.
基金supported by the Sichuan Provincial Department of Science and Technology(2024ZHCG0086)the Chongqing Science and Technology Commission(cstc2021jscx-cylhX0014).
文摘Chili fermentation is one of the most important processes in the production of Pixian Douban(PXDB),which determines the flavor and product quality of PXDB.However,the maturity of fermented chili mainly depends on empirical judgment,which can not satisfy the need for standardized production of PXDB.Therefore,this study aimed to investigate volatile and non-volatile substances during the maturation process and to find markers related to the maturity of fermented chili.Two-dimensional gas chromatography-mass spectrometry(GC×GC-MS)combined with multivariate statistical analysis and relative odor activity values(ROAV)analysis revealed that 2-methoxy-3-isobutyl pyrazine,linalool,3-(methylthio)propionaldehyde,myrcene,and decanal(ROAV≥1,VIP>1,P<0.05)were regarded as potentially active aromatic markers for differentiating fermentation time.Additionally,ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLCQTOF-MS)combined with multivariate statistical analysis revealed that 25 compounds could serve as differentiated non-volatile compounds.The correlation of maturity-related physicochemical indicators with volatile and nonvolatile compounds revealed that four volatile compounds(2-methoxy-3-isobutylpyrazine,linalool,myrcene,and decanal)along with seven non-volatile compounds could serve as markers for evaluating the maturity of fermented chili.This study is expected to establish a standard for the determination of the maturity the fermented chili and lay the foundation for intelligent production of PXDB.
文摘Objectives:B-cell maturation antigen(BCMA)-targeted antibody–drug conjugates(ADCs)have emerged as promising therapies for relapsed/refractory multiple myeloma(RRMM),but the overall efficacy and safety profile is unclear.This study aimed to synthesize the available evidence on the safety and efficacy of BCMA-ADCs in development for RRMM.Methods:A systematic search was conducted using six bibliographic databases and ClinicalTrials.gov up to November 2024.Studies were eligible if they were human clinical trials or animal studies evaluating BCMA-ADCs and reported efficacy and safety outcomes.Data extraction and quality assessments were conducted using validated tools,including ROBINS-I and SYRCLE’s risk of bias tool.Results:A total of 21 studies were included:16 clinical trials and five animal studies.Key findings included that belantamab mafodotin demonstrated variable but generally durable response rates(32%–85%)and a broad range of progression-free survival(PFS)(2.8–36.6 months),albeit with ocular toxicities in 51%–96%.Among newer candidates,MEDI2228 showed median PFS 5.1–6.6 months with 14%discontinuation for ocular symptoms,while AMG 224 had an overall response rate(ORR)of 23%(9/40)with anemia 21%,thrombocytopenia 24%,and ocular adverse events(AEs)21%.Animal studies supported the tumor-eradicating potential of all BCMA-ADC candidates,although safety signals such as hepatic and renal toxicity were noted with HDP-101.The risk of bias assessment revealed generally moderate to serious concerns in human trials,while the overall quality of the animal studies was acceptable.Conclusions:BCMA-targeted ADC candidates show encouraging efficacy in RRMM,particularly belantamab mafodotin.However,frequent AEs,especially ocular and hematologic toxicities,underscore the need for optimization in ADC design.Further research should prioritize enhancing safety while maintaining clinical benefit.
基金supported by funding from the National Natural Science Foundation of China(81971349 and 81300456).
文摘In vitro maturation(IvM)of human oocytes offers cost efficiency and minimal invasiveness,serving as a valuable supplementary tool in assisted reproduction for fertility preservation,ovarian hyperstimulation syndrome prevention,and other reproductive strategies.Despite its availability for three decades,the clinical use of IVM remains limited due to efficacy and safety concerns.This study examines the DNA methylation profile of IVM oocytes collected during laparoscopic/hysteroscopic surgeries compared to in vivo matured oocytes via reduced representation bisulfite sequencing.Results indicate IVM oocytes exhibit a higher global methylation level.Differentially methylated regions(DMRs)analysis reveals that the in vitro group displays more hypermethylated and fewer hypomethylated DMRs compared to the in vivo group.Additionally,the in vitro group exhibits a higher level of non-CpG methylation than the in vivo group.However,no significant correlation between methylation levels and transcriptional activity in these oocytes is found,especially for those specific imprinted genes or genes related to embryonic development.These findings shed light on the epigenetic landscape of IvM oocytes,contributing to the ongoing assessment of their clinical feasibility and safety in assisted reproduction.
