The burr is one of the common phenomena occurring i n metal cutting operations The mathematical mechanical model of two side dir ection burr formation and transformation is established with plane stress strain th...The burr is one of the common phenomena occurring i n metal cutting operations The mathematical mechanical model of two side dir ection burr formation and transformation is established with plane stress strain theory,based on the orthogonal cutting The main laws of formation and change of the burr are revealed,and it is confirmed by experiment result,which first realizes prediction of the forming and changing of the two side direction burr in metal cutting operation.展开更多
Gambogic acid （GA） is an anticancer agent in phase IIb clinical trial in China but its mechanism of action has not been fully clarified. The present study was designed to search the possible target-related proteins ...Gambogic acid （GA） is an anticancer agent in phase IIb clinical trial in China but its mechanism of action has not been fully clarified. The present study was designed to search the possible target-related proteins of GA in cancer cells using proteomic method and establish possible network using bioinformatic analysis. Cytotoxicity and anti-migration effects of GA in MDA-MB-231 cells were checked using MTT assay, flow cytometry, wound migration assay, and chamber migration assay. Possible target-related proteins of GA at early （3 h） and late stage （24 h） of treatment were searched using a proteomic technology, two-dimensional electro- phoresis （2-DE）. The possible network of GA was established using bioinformatic analysis. The intracellular expression levels of vimentin, keratin 18, and calumenin were determined using Western blotting. GA inhibited cell proliferation and induced cell cycle arrest at G2/M phase and apoptosis in MDA-MB-231 cells. Additionally, GA exhibited anti-migration effects at non-toxic doses. In 2-DE analysis, totally 23 possible GA targeted proteins were found, including those with functions in cytoskeleton and transport, regulation of redox state, metabolism, ubiquitin-proteasome system, transcription and translation, protein transport and modification, and cytokine. Network analysis of these proteins suggested that cytoskeleton-related proteins might play important roles in the effects of GA. Results of Western blotting confirmed the cleavage of vimentin, increase in keratin 18, and decrease in calumenin levels in GA-treated cells. In summary, GA is a multi-target compound and its anti-cancer effects may be based on several target-related pro- teins such as cytoskeleton-related proteins.展开更多
Circadian rhythms have a period of~24 h and are manifested in almost all living organisms [1].The discovery of the molecular genetic mechanisms underlying circadian rhythmicity was one of the most exciting and inspiri...Circadian rhythms have a period of~24 h and are manifested in almost all living organisms [1].The discovery of the molecular genetic mechanisms underlying circadian rhythmicity was one of the most exciting and inspiring chapters in the history of biology and won the Nobel Prize in Physiology or Medicine in 2017 [2].Equally exciting and inspiring was the discovery that the hypothalamic suprachiasmatic nucleus (SCN) is involved in orchestrating the daily rhythmic activity of physiology。展开更多
In order to solve the problem of intraperitoneal haemorrhage and bile leakage caused by the iatrogenic trauma, sixty-four sequence of puncture were performed on twenty canine livers and thirty-two sequences selected b...In order to solve the problem of intraperitoneal haemorrhage and bile leakage caused by the iatrogenic trauma, sixty-four sequence of puncture were performed on twenty canine livers and thirty-two sequences selected by chance were perfused with thrombin solution. The results revealed that the bleeding time was shortened and amounts lessened significantly after application of thrombin compared with counterparts of control (P< 0. 001,t test).There was no significant difference between the levels of plasma fibringgeu prc-and Post-thrombin's using (P > 0.4,t test).The thrombin clotting time of patients with obstructive jaundice was similar to that of the group of control(P> 0. 05, t test).We conclude that thrombin is characterized by the ability of rapid,effective and safe haemostasis to the traumatic channel made by the liver puncture,and it's adaptive to most of the patients with hepatobiliary disease,and worth using and developing in clinical practice.展开更多
We present high quality long slit spectra along the major and minor axes out to 1.5 effective radii of the massive galaxy NGC 4636 taken by the Hobby-Eberly Telescope. Using the Fourier Correlation Quotient method, we...We present high quality long slit spectra along the major and minor axes out to 1.5 effective radii of the massive galaxy NGC 4636 taken by the Hobby-Eberly Telescope. Using the Fourier Correlation Quotient method, we measured the stellar line-of-sight velocity distribution along the axes. Furthermore, six Lick/IDS indices （Hβ, Mgb, Fe5015, Fe5270, Fe5335, Fe5406） are derived from the clean spectrum. By comparing the measured absorption line strengths with the predictions of Simple Stellar Population （SSP） models, we derived ages, total metallicity and a abundance profiles of the galaxy. This galaxy presents old and [α/Fe] overabundant stellar populations. Indeed, using the SSP model, we obtained the broadband color profiles. The theoretical colors match well with the measured colors and present red sharp peaks at the galaxy center. The sharp peaks of the colors are mainly shaped by the high metallicity in the galaxy＇s center. Interestingly, the galaxy has steep negative metaUicity gradients, but the trend flattens outwards. This result likely suggests that the center and outer regions of the galaxy formed through different formation processes.展开更多
基金This project is supported by National Natural Science Foundation of China(No.59775071).
文摘The burr is one of the common phenomena occurring i n metal cutting operations The mathematical mechanical model of two side dir ection burr formation and transformation is established with plane stress strain theory,based on the orthogonal cutting The main laws of formation and change of the burr are revealed,and it is confirmed by experiment result,which first realizes prediction of the forming and changing of the two side direction burr in metal cutting operation.
基金supported by the Twelfth Five-Year National Science & Technology Support Program(No.2012BAI 29B06)Shanghai Science & Technology Support Program(No.13431900 401)+4 种基金China Postdoctoral Science Foundation Funded Project(No.2012M5 10907)Shanghai Postdoctoral Scientific Program(No.13R21417800)the Postdoctor Research Program of Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences(No.2012KIP516)the Sanofi-Aventis-Shanghai Institutes for Biological Sciences Scholarship Programthe National Nature Science Foundation(Nos.81302809 and 81373964)
文摘Gambogic acid （GA） is an anticancer agent in phase IIb clinical trial in China but its mechanism of action has not been fully clarified. The present study was designed to search the possible target-related proteins of GA in cancer cells using proteomic method and establish possible network using bioinformatic analysis. Cytotoxicity and anti-migration effects of GA in MDA-MB-231 cells were checked using MTT assay, flow cytometry, wound migration assay, and chamber migration assay. Possible target-related proteins of GA at early （3 h） and late stage （24 h） of treatment were searched using a proteomic technology, two-dimensional electro- phoresis （2-DE）. The possible network of GA was established using bioinformatic analysis. The intracellular expression levels of vimentin, keratin 18, and calumenin were determined using Western blotting. GA inhibited cell proliferation and induced cell cycle arrest at G2/M phase and apoptosis in MDA-MB-231 cells. Additionally, GA exhibited anti-migration effects at non-toxic doses. In 2-DE analysis, totally 23 possible GA targeted proteins were found, including those with functions in cytoskeleton and transport, regulation of redox state, metabolism, ubiquitin-proteasome system, transcription and translation, protein transport and modification, and cytokine. Network analysis of these proteins suggested that cytoskeleton-related proteins might play important roles in the effects of GA. Results of Western blotting confirmed the cleavage of vimentin, increase in keratin 18, and decrease in calumenin levels in GA-treated cells. In summary, GA is a multi-target compound and its anti-cancer effects may be based on several target-related pro- teins such as cytoskeleton-related proteins.
基金supported by the National Key R&D Program of China(2019YFA0802400)the National Natural Science Foundation of China(31961133026,31871187,and 81570171)the Priority Academic Program Development of Jiangsu Higher Education Institutions(YX13400214)。
文摘Circadian rhythms have a period of~24 h and are manifested in almost all living organisms [1].The discovery of the molecular genetic mechanisms underlying circadian rhythmicity was one of the most exciting and inspiring chapters in the history of biology and won the Nobel Prize in Physiology or Medicine in 2017 [2].Equally exciting and inspiring was the discovery that the hypothalamic suprachiasmatic nucleus (SCN) is involved in orchestrating the daily rhythmic activity of physiology。
文摘In order to solve the problem of intraperitoneal haemorrhage and bile leakage caused by the iatrogenic trauma, sixty-four sequence of puncture were performed on twenty canine livers and thirty-two sequences selected by chance were perfused with thrombin solution. The results revealed that the bleeding time was shortened and amounts lessened significantly after application of thrombin compared with counterparts of control (P< 0. 001,t test).There was no significant difference between the levels of plasma fibringgeu prc-and Post-thrombin's using (P > 0.4,t test).The thrombin clotting time of patients with obstructive jaundice was similar to that of the group of control(P> 0. 05, t test).We conclude that thrombin is characterized by the ability of rapid,effective and safe haemostasis to the traumatic channel made by the liver puncture,and it's adaptive to most of the patients with hepatobiliary disease,and worth using and developing in clinical practice.
基金Supported by the National Natural Science Foundation of China rant No. 10821061the National Basic Research Program of China (Grant Nos. 11033008 nd 2007CB815406)the pport of the Chinese Academy of Sciences (Grant No. KJCX2-YW-T24)
文摘We present high quality long slit spectra along the major and minor axes out to 1.5 effective radii of the massive galaxy NGC 4636 taken by the Hobby-Eberly Telescope. Using the Fourier Correlation Quotient method, we measured the stellar line-of-sight velocity distribution along the axes. Furthermore, six Lick/IDS indices （Hβ, Mgb, Fe5015, Fe5270, Fe5335, Fe5406） are derived from the clean spectrum. By comparing the measured absorption line strengths with the predictions of Simple Stellar Population （SSP） models, we derived ages, total metallicity and a abundance profiles of the galaxy. This galaxy presents old and [α/Fe] overabundant stellar populations. Indeed, using the SSP model, we obtained the broadband color profiles. The theoretical colors match well with the measured colors and present red sharp peaks at the galaxy center. The sharp peaks of the colors are mainly shaped by the high metallicity in the galaxy＇s center. Interestingly, the galaxy has steep negative metaUicity gradients, but the trend flattens outwards. This result likely suggests that the center and outer regions of the galaxy formed through different formation processes.
