目的:通过对C3H10T1/2细胞分化为成熟棕色脂肪细胞的培养、诱导分化及鉴定,深入探讨棕色脂肪细胞的生物学特性,为人类棕色脂肪细胞的相关研究提供实验参考与理论依据。方法:C3H10T1/2细胞经细胞接种、培养及诱导分化处理,利用光学显微...目的:通过对C3H10T1/2细胞分化为成熟棕色脂肪细胞的培养、诱导分化及鉴定,深入探讨棕色脂肪细胞的生物学特性,为人类棕色脂肪细胞的相关研究提供实验参考与理论依据。方法:C3H10T1/2细胞经细胞接种、培养及诱导分化处理,利用光学显微镜观察细胞形态变化,并通过油红O染色、免疫荧光法、线粒体探针法以及线粒体电镜技术对分化细胞进行鉴定分析。结果:未分化的C3H10T1/2细胞形态多样,具有突触伸展特征;分化后的细胞逐渐变为圆形或椭圆形,形成环形脂滴;未分化组细胞形态无明显变化。油红O染色显示,未分化组细胞基本无染色,而分化组中约90%的细胞红染,脂滴分布于细胞核周围,吸光度值显著升高(P<0.05)。分化组解偶联蛋白1(uncoupling protein 1,UCP1)、过氧化物酶体增殖激活受体γ(peroxisome proliferator-activated receptorγ,PPARγ)辅激活因子1α(PPARγco-activator-1α,PGC-1α)、PPARγ和转录因子PRDM16的相对荧光强度和蛋白表达量显著高于未分化组(P<0.05),且线粒体活性增强。结论:本研究成功诱导C3H10T1/2细胞分化为成熟棕色脂肪细胞,结合细胞形态观察、关键蛋白表达检测及线粒体功能分析进行综合评估,证实了细胞的有效分化及成熟棕色脂肪细胞的功能特性。展开更多
This paper proposes an adaptive synchronization problem for the master and slave structure of linear systems with nonlinear perturbations and mixed time-varying delays comprising different discrete and distributed tim...This paper proposes an adaptive synchronization problem for the master and slave structure of linear systems with nonlinear perturbations and mixed time-varying delays comprising different discrete and distributed time delays. Using an appropriate Lyapunov-Krasovskii functional, some delay-dependent sufficient conditions and an adaptation law including the master-slave parame- ters are established for designing a delayed synchronization law in terms of linear matrix inequalities（LMIs）. The time-varying controller guarantees the H ∞ synchronization of the two coupled master and slave systems regardless of their initial states. Particularly, it is shown that the synchronization speed can be controlled by adjusting the updated gain of the synchronization signal. Two numerical examples are given to demonstrate the effectiveness of the method.展开更多
本文旨在探究富含天冬氨酸尾1的单通道膜蛋白(Single-pass Membrane Protein With Aspartate Rich Tail1,Smdt1)对C3H10T1/2细胞增殖和成脂分化的调控效应。本研究将Smdt1基因的过表达和干扰载体转染至C3H10T1/2细胞模型,采用qPCR方法...本文旨在探究富含天冬氨酸尾1的单通道膜蛋白(Single-pass Membrane Protein With Aspartate Rich Tail1,Smdt1)对C3H10T1/2细胞增殖和成脂分化的调控效应。本研究将Smdt1基因的过表达和干扰载体转染至C3H10T1/2细胞模型,采用qPCR方法量化了增殖和成脂分化关键基因的表达水平变化,利用EdU染色检测细胞增殖活力,油红O染色方法鉴定脂滴积累的状态;进一步通过String database、Bio GRID、Int Act、GeneMANIA、DAVID和Genecard数据库构建Smdt1蛋白互作网络图。结果显示,在C3H10T1/2细胞中过表达Smdt1,极显著提升了增殖标志基因Pcna、Ki67、Cdk1及Cdk4的表达,EdU阳性细胞比例反映了细胞增殖速率加快;Smdt1极显著促进成脂分化关键基因Pparγ、Fabp4、-Adipoq的表达量,显著促进了Cebpα、Cebpβ的表达量,脂滴数量变多。在C3H10T1/2细胞体系中,干扰Smdt1,与增殖紧密相关的标志基因,包括Ki67、Pcna及Cdk1,其表达水平极显著降低,Cdk4的表达也呈现显著降低的趋势,反映在EdU增殖检测中,阳性细胞数量明显减少,细胞增殖活性受到抑制。进一步干扰Smdt1后,成脂分化途径的关键调控基因Cebpα、Pparγ、Cebpβ、Fabp4、Adipoq的表达均极显著降低,细胞内脂滴的数量也显著减少,细胞成脂分化能力削弱。蛋白功能预测发现,Smdt1能够与Mcu相互作用,参与线粒体钙离子转运、摄取和稳态。本研究发现Smdt1可以促进C3H10T1/2细胞增殖和成脂分化,为脂肪沉积的研究提供了新的方向。展开更多
文摘目的:通过对C3H10T1/2细胞分化为成熟棕色脂肪细胞的培养、诱导分化及鉴定,深入探讨棕色脂肪细胞的生物学特性,为人类棕色脂肪细胞的相关研究提供实验参考与理论依据。方法:C3H10T1/2细胞经细胞接种、培养及诱导分化处理,利用光学显微镜观察细胞形态变化,并通过油红O染色、免疫荧光法、线粒体探针法以及线粒体电镜技术对分化细胞进行鉴定分析。结果:未分化的C3H10T1/2细胞形态多样,具有突触伸展特征;分化后的细胞逐渐变为圆形或椭圆形,形成环形脂滴;未分化组细胞形态无明显变化。油红O染色显示,未分化组细胞基本无染色,而分化组中约90%的细胞红染,脂滴分布于细胞核周围,吸光度值显著升高(P<0.05)。分化组解偶联蛋白1(uncoupling protein 1,UCP1)、过氧化物酶体增殖激活受体γ(peroxisome proliferator-activated receptorγ,PPARγ)辅激活因子1α(PPARγco-activator-1α,PGC-1α)、PPARγ和转录因子PRDM16的相对荧光强度和蛋白表达量显著高于未分化组(P<0.05),且线粒体活性增强。结论:本研究成功诱导C3H10T1/2细胞分化为成熟棕色脂肪细胞,结合细胞形态观察、关键蛋白表达检测及线粒体功能分析进行综合评估,证实了细胞的有效分化及成熟棕色脂肪细胞的功能特性。
文摘This paper proposes an adaptive synchronization problem for the master and slave structure of linear systems with nonlinear perturbations and mixed time-varying delays comprising different discrete and distributed time delays. Using an appropriate Lyapunov-Krasovskii functional, some delay-dependent sufficient conditions and an adaptation law including the master-slave parame- ters are established for designing a delayed synchronization law in terms of linear matrix inequalities（LMIs）. The time-varying controller guarantees the H ∞ synchronization of the two coupled master and slave systems regardless of their initial states. Particularly, it is shown that the synchronization speed can be controlled by adjusting the updated gain of the synchronization signal. Two numerical examples are given to demonstrate the effectiveness of the method.
文摘本文旨在探究富含天冬氨酸尾1的单通道膜蛋白(Single-pass Membrane Protein With Aspartate Rich Tail1,Smdt1)对C3H10T1/2细胞增殖和成脂分化的调控效应。本研究将Smdt1基因的过表达和干扰载体转染至C3H10T1/2细胞模型,采用qPCR方法量化了增殖和成脂分化关键基因的表达水平变化,利用EdU染色检测细胞增殖活力,油红O染色方法鉴定脂滴积累的状态;进一步通过String database、Bio GRID、Int Act、GeneMANIA、DAVID和Genecard数据库构建Smdt1蛋白互作网络图。结果显示,在C3H10T1/2细胞中过表达Smdt1,极显著提升了增殖标志基因Pcna、Ki67、Cdk1及Cdk4的表达,EdU阳性细胞比例反映了细胞增殖速率加快;Smdt1极显著促进成脂分化关键基因Pparγ、Fabp4、-Adipoq的表达量,显著促进了Cebpα、Cebpβ的表达量,脂滴数量变多。在C3H10T1/2细胞体系中,干扰Smdt1,与增殖紧密相关的标志基因,包括Ki67、Pcna及Cdk1,其表达水平极显著降低,Cdk4的表达也呈现显著降低的趋势,反映在EdU增殖检测中,阳性细胞数量明显减少,细胞增殖活性受到抑制。进一步干扰Smdt1后,成脂分化途径的关键调控基因Cebpα、Pparγ、Cebpβ、Fabp4、Adipoq的表达均极显著降低,细胞内脂滴的数量也显著减少,细胞成脂分化能力削弱。蛋白功能预测发现,Smdt1能够与Mcu相互作用,参与线粒体钙离子转运、摄取和稳态。本研究发现Smdt1可以促进C3H10T1/2细胞增殖和成脂分化,为脂肪沉积的研究提供了新的方向。
