[Objectives] The research aimed to investigate the genetic rule of the disease resistance of tomato CLN2037,find molecular markers linked with disease-resistant genes and locate the disease-resistant genes. [Methods] ...[Objectives] The research aimed to investigate the genetic rule of the disease resistance of tomato CLN2037,find molecular markers linked with disease-resistant genes and locate the disease-resistant genes. [Methods] BCr,BCs and F2 were constructed from tomato CLN2037 and susceptible cultivar T2-03,and their resistance to late blight was identified and analyzed by inoculating the disease in the seedling stage of tomatoes. The genetic linkage analysis was made based on the genetic linkage map constructed by ICu GI using group segregation method and 90 pairs of tomato SSR primers. [Results]The resistance of CLN2037 to late blight was controlled by a single recessive gene. The polymorphic bands amplified by primer DM0231 had a linkage relationship with resistance genes. The size of the polymorphism fragment was 226 bp with the genetic linkage distance of 2. 67 c M,and the disease resistance gene was localized on CCPB272-03741.[Conclusions]DM0231 could be used as a molecular marker for molecular breeding of tomato against late blight,which laid the foundation for further fine localization of late blight resistance genes.展开更多
基金Supported by the Science and Technology Pilot Project of Xinjiang Academy of Agricultural Reclamation Science(50YYD201610)
文摘[Objectives] The research aimed to investigate the genetic rule of the disease resistance of tomato CLN2037,find molecular markers linked with disease-resistant genes and locate the disease-resistant genes. [Methods] BCr,BCs and F2 were constructed from tomato CLN2037 and susceptible cultivar T2-03,and their resistance to late blight was identified and analyzed by inoculating the disease in the seedling stage of tomatoes. The genetic linkage analysis was made based on the genetic linkage map constructed by ICu GI using group segregation method and 90 pairs of tomato SSR primers. [Results]The resistance of CLN2037 to late blight was controlled by a single recessive gene. The polymorphic bands amplified by primer DM0231 had a linkage relationship with resistance genes. The size of the polymorphism fragment was 226 bp with the genetic linkage distance of 2. 67 c M,and the disease resistance gene was localized on CCPB272-03741.[Conclusions]DM0231 could be used as a molecular marker for molecular breeding of tomato against late blight,which laid the foundation for further fine localization of late blight resistance genes.
