Edible plants are increasingly recognised as promising nutraceutical options for various diseases,including cancer.We were the first to discover that Crithmum maritimum L.,an edible wild plant,inhibits the growth of h...Edible plants are increasingly recognised as promising nutraceutical options for various diseases,including cancer.We were the first to discover that Crithmum maritimum L.,an edible wild plant,inhibits the growth of hepatocellular carcinoma cells by targeting multiple metabolic pathways,such as lactic acid fermentation and biosynthetic pathways characteristic of tumour cells.In this study,we investigated the effects of ethyl acetate extract of Crithmum maritimum on the growth inhibition of four different cancer types:colon,pancreatic,breast,and glioblastoma.Our results demonstrate that Crithmum maritimum significantly suppresses the in vitro growth of all four tumour types by inducing a metabolic shift from fermentation to respiration,which correlates with improved metabolic enzyme markers.In particular,we demonstrated that Crithmum maritimum reduces lactic acid fermentation by decreasing the gene expression ratio of two lactate dehydrogenase isoforms(LDHA/LDHB),thereby lowering the production of L-lactate.We also showed that Crithmum maritimum promotes the restoration of a more normal cellular redox potential by enhancing NAD^(+)production at the expense of NADH.Furthermore,we observed Crithmum maritimum-mediated recovery of mitochondrial function through stimulation of cellular respiration and modulation of the gene expression of cytochrome c oxidase subunits(COXs),specifically COX I,COX IV-1,and COX IV-2.Notably,we described the anti-inflammatory and anti-cholesterolaemic effects of Crithmum maritimum.Taken together,our findings underscore the potential of Crithmum maritimum L.as a valuable nutraceutical supplement for conventional pharmacological therapies for various types of tumours.展开更多
Objective: To examine the antioxidant potential of Tunisian Eryngium maritimum (E. maritimum) leaf, root and stems extracts, as well as their phenolic compositions. Methods: The antioxidant activity of different extra...Objective: To examine the antioxidant potential of Tunisian Eryngium maritimum (E. maritimum) leaf, root and stems extracts, as well as their phenolic compositions. Methods: The antioxidant activity of different extracts was assessed using DPPH free radical and hydrogen peroxide scavenging assays. Phenolic profiles were determined by means of liquid chromatography (HPLC-DAD). Results: All plant parts were a rich source of phenolics. Polyphenols and flavonoids were present in leaf extracts. E. maritimum leaf extracts displayed the strongest H2O2 scavenging activity (IC50 = 76.83 μg/mL) and the highest DPPH scavenging activity value (IC50 = 47.87 μg/mL) compared to other extracts. Good relationships were observed between antioxidant activities and the total phenolic and flavonoid contents. Nine bioactive compounds were detected in E. maritimum extracts. Conclusions: Our results provided evidence that E. maritimum could be an interesting source of natural antioxidant that can be used to treat divers diseases.展开更多
The present study examined the antilithiasic activity of the hydromethanolic extract of Polygonum maritimum aerial parts(Pm).The chromatographic analysis made it possible to identify 30 compounds,namely gallic acid,(+...The present study examined the antilithiasic activity of the hydromethanolic extract of Polygonum maritimum aerial parts(Pm).The chromatographic analysis made it possible to identify 30 compounds,namely gallic acid,(+)-catechin,three isomers of myricetin deoxyhexoside,quercetin pentoside,and quercitrin as the major molecules.The microscopic and macroscopic observations together with the plasma and urinary parameters as well as the histological analysis revealed that the use of Pm extracts(37.5 and 50 mg/kg)during 15 days significantly improved the elevated pH,hyperoxaluria,hypercalcemia,and decreased the urinary volume,when compared to the ethylene glycol group(EG-group).In addition,the microscopic examination revealed that the large crystals and oxalic aggregates detected in urinary,induced by EG(ethylene glycol),were mitigated by Pm-administration.Furthermore,the Pm supplement significantly restored the levels of urea,uric acid,and creatinine,and also led to a strong diuresis which helped remove crystal debris.These results were reinforced by the histological study.The current study highlighted the potential use of Pm for its remarkable beneficial effect during the various lithogenic stages as well as for improving kidney function.展开更多
基金supported by the“Tecnonidi”project,promoted by Regione Pugliafunded by Olivhealth Srl,Italy.
文摘Edible plants are increasingly recognised as promising nutraceutical options for various diseases,including cancer.We were the first to discover that Crithmum maritimum L.,an edible wild plant,inhibits the growth of hepatocellular carcinoma cells by targeting multiple metabolic pathways,such as lactic acid fermentation and biosynthetic pathways characteristic of tumour cells.In this study,we investigated the effects of ethyl acetate extract of Crithmum maritimum on the growth inhibition of four different cancer types:colon,pancreatic,breast,and glioblastoma.Our results demonstrate that Crithmum maritimum significantly suppresses the in vitro growth of all four tumour types by inducing a metabolic shift from fermentation to respiration,which correlates with improved metabolic enzyme markers.In particular,we demonstrated that Crithmum maritimum reduces lactic acid fermentation by decreasing the gene expression ratio of two lactate dehydrogenase isoforms(LDHA/LDHB),thereby lowering the production of L-lactate.We also showed that Crithmum maritimum promotes the restoration of a more normal cellular redox potential by enhancing NAD^(+)production at the expense of NADH.Furthermore,we observed Crithmum maritimum-mediated recovery of mitochondrial function through stimulation of cellular respiration and modulation of the gene expression of cytochrome c oxidase subunits(COXs),specifically COX I,COX IV-1,and COX IV-2.Notably,we described the anti-inflammatory and anti-cholesterolaemic effects of Crithmum maritimum.Taken together,our findings underscore the potential of Crithmum maritimum L.as a valuable nutraceutical supplement for conventional pharmacological therapies for various types of tumours.
文摘Objective: To examine the antioxidant potential of Tunisian Eryngium maritimum (E. maritimum) leaf, root and stems extracts, as well as their phenolic compositions. Methods: The antioxidant activity of different extracts was assessed using DPPH free radical and hydrogen peroxide scavenging assays. Phenolic profiles were determined by means of liquid chromatography (HPLC-DAD). Results: All plant parts were a rich source of phenolics. Polyphenols and flavonoids were present in leaf extracts. E. maritimum leaf extracts displayed the strongest H2O2 scavenging activity (IC50 = 76.83 μg/mL) and the highest DPPH scavenging activity value (IC50 = 47.87 μg/mL) compared to other extracts. Good relationships were observed between antioxidant activities and the total phenolic and flavonoid contents. Nine bioactive compounds were detected in E. maritimum extracts. Conclusions: Our results provided evidence that E. maritimum could be an interesting source of natural antioxidant that can be used to treat divers diseases.
基金The authors express their appreciation to the Researchers Supporting Project(RSP 2023R17)at King Saud University,Riyadh,Saudi Arabiathe Ministry of Science and Innovation of Spain for the Ram´on y Cajal grant RYC2020-030546-I/AEI/10.13039/501100011033 and the European Social Fund。
文摘The present study examined the antilithiasic activity of the hydromethanolic extract of Polygonum maritimum aerial parts(Pm).The chromatographic analysis made it possible to identify 30 compounds,namely gallic acid,(+)-catechin,three isomers of myricetin deoxyhexoside,quercetin pentoside,and quercitrin as the major molecules.The microscopic and macroscopic observations together with the plasma and urinary parameters as well as the histological analysis revealed that the use of Pm extracts(37.5 and 50 mg/kg)during 15 days significantly improved the elevated pH,hyperoxaluria,hypercalcemia,and decreased the urinary volume,when compared to the ethylene glycol group(EG-group).In addition,the microscopic examination revealed that the large crystals and oxalic aggregates detected in urinary,induced by EG(ethylene glycol),were mitigated by Pm-administration.Furthermore,the Pm supplement significantly restored the levels of urea,uric acid,and creatinine,and also led to a strong diuresis which helped remove crystal debris.These results were reinforced by the histological study.The current study highlighted the potential use of Pm for its remarkable beneficial effect during the various lithogenic stages as well as for improving kidney function.
