As it was rear to find out detailed description, a study on biology and control measures ofP. marginatus was carried out at National Plant Quarantine Service, Katunayake Sri Lanka. Average length and width of differen...As it was rear to find out detailed description, a study on biology and control measures ofP. marginatus was carried out at National Plant Quarantine Service, Katunayake Sri Lanka. Average length and width of different stages, number of eggs in an egg sac, hatchability rate and duration of life cycle were studied. Control measures were tested using herbal oils and it was arranged in Completely Randomized Design with six replicates. Experiments were carried out in laboratory conditions under 28 ±2℃ and 70% RH. Range of length and width of different life stages revealed that, egg 0.3-0.1 mm ×0.15-0.10 mm, 1st instar 0.4-0.2 mm × 0.20-0.10 mm, 2nd instar 0.6-0.5 mm × 0.29-0.20 mm, 3rd instar male 0.8-0.5 mm × 0.30-0.20 mm, 3rd instar female 0.7-0.5 mm × 0.29-0.20 mm, adult male 0.9-0.7 mm× 0.20-0.10 mm and adult female 2.8-1.9 mm × 1.40-0.80 mm. A range of 100-200 eggs were in an ovisac and hatchability rate was 76-80%. Twenty to twenty-four days were taken to complete their life cycle. Cinnamon and Neem oil in cooperated with Surfactant and Kerosene oil could be effectively used as potential chemical agents for control of P. marginatus.展开更多
This study was to establish a molecular identification method to distinguish Dermacentor nuttalli(D. nuttalli) and Dermacentor marginatus(D. marginatus),and to study their phylogenetic relationship. The ticks were col...This study was to establish a molecular identification method to distinguish Dermacentor nuttalli(D. nuttalli) and Dermacentor marginatus(D. marginatus),and to study their phylogenetic relationship. The ticks were collected from domestic animals in Xinjiang Uygur Autonomous Region for morphological identification,then their genomic DNAs were isolated for amplifying mitochondrial 16 S rRNA and cytochrome oxidase subunit I gene(COI). The phylogenetic tree was constructed based on the sequencing results of PCR products by employing Mega 5. 0 and Mrbayes 3. 2 for homology analysis. Clustering analysis PCR product for 16 S rRNA from both D. marginatus and D. nuttalli was clustered together with their respective 16 S rRNA sequences previously accessed in GenBank,and that for COI gene as well. These are basically identical with morphological identification results. Our results indicate that morphological identification,combined with molecular markers,would be a simple and accurate method for distinguishing D. nuttalli and D. marginatus.展开更多
用磁珠-生物素标记的微卫星探针(ACA)15与白缘缺(Leiobagrus marginatus)基因组酶切片段杂交,捕获含有微卫星序列的DNA片段,连接到T载体中,构建富集微卫星序列的小片段插入文库,再用γ-32P标记的探针进行二次筛选,获得阳性克隆785个,对...用磁珠-生物素标记的微卫星探针(ACA)15与白缘缺(Leiobagrus marginatus)基因组酶切片段杂交,捕获含有微卫星序列的DNA片段,连接到T载体中,构建富集微卫星序列的小片段插入文库,再用γ-32P标记的探针进行二次筛选,获得阳性克隆785个,对其中的140个克隆进行了测序,获得完整的微卫星序列132个．用引物设计软件Primer Premier 5．0设计引物64对．对其中的20对引物进行了多态性检测,筛选到稳定扩增的标记13对,用此13对微卫星引物分析了缺属白缘缺(L．marginalus)、拟缘缺(L．marginatoides)(大、小各1种)、黑尾缺(L．nigricauda)共4个群体的遗传多样性,其中10对微卫星引物在种内或种间表现出多态性．展开更多
【目的】木瓜秀粉蚧Paracoccus marginatus Williams and Granara de Willink是我国一种重要的新入侵害虫,为筛选出防治木瓜秀粉蚧的高效药剂,明确其对不同杀虫剂的敏感性。【方法】采用浸叶法测定了2龄若虫和雌成虫对15种常用杀虫剂的...【目的】木瓜秀粉蚧Paracoccus marginatus Williams and Granara de Willink是我国一种重要的新入侵害虫,为筛选出防治木瓜秀粉蚧的高效药剂,明确其对不同杀虫剂的敏感性。【方法】采用浸叶法测定了2龄若虫和雌成虫对15种常用杀虫剂的敏感性。【结果】从15种药剂中筛选出5种对木瓜秀粉蚧毒力较高的杀虫剂,分别为25 g·L^-1高效氯氟氰菊酯乳油、10%虫螨腈悬浮剂、4.5%高效氯氰菊酯乳油、15%哒螨灵水乳剂和15%唑虫酰胺悬浮剂;其中,25 g·L^-1高效氯氟氰菊酯乳油对木瓜秀粉蚧的致死中浓度(LC50)最小,2龄若虫和雌成虫的致死中浓度LC50分别为0.243 0、0.782 1 mg·L^-1,其次为10%虫螨腈悬浮剂、4.5%高效氯氰菊酯乳油和15%哒螨灵水乳剂,15%唑虫酰胺悬浮剂的LC50值最大;木瓜秀粉蚧2龄若虫对药剂的敏感性明显高于雌成虫。【结论】就生物活性测定而言,在木瓜秀粉蚧爆发时,可选用25 g·L^-1高效氯氟氰菊酯乳油、10%虫螨腈悬浮剂、4.5%高效氯氰菊酯乳油、15%哒螨灵水乳剂和15%唑虫酰胺悬浮剂作为当前防治木瓜秀粉蚧的主要防治药剂,可有效遏制入侵害虫木瓜秀粉蚧的危害。展开更多
文摘As it was rear to find out detailed description, a study on biology and control measures ofP. marginatus was carried out at National Plant Quarantine Service, Katunayake Sri Lanka. Average length and width of different stages, number of eggs in an egg sac, hatchability rate and duration of life cycle were studied. Control measures were tested using herbal oils and it was arranged in Completely Randomized Design with six replicates. Experiments were carried out in laboratory conditions under 28 ±2℃ and 70% RH. Range of length and width of different life stages revealed that, egg 0.3-0.1 mm ×0.15-0.10 mm, 1st instar 0.4-0.2 mm × 0.20-0.10 mm, 2nd instar 0.6-0.5 mm × 0.29-0.20 mm, 3rd instar male 0.8-0.5 mm × 0.30-0.20 mm, 3rd instar female 0.7-0.5 mm × 0.29-0.20 mm, adult male 0.9-0.7 mm× 0.20-0.10 mm and adult female 2.8-1.9 mm × 1.40-0.80 mm. A range of 100-200 eggs were in an ovisac and hatchability rate was 76-80%. Twenty to twenty-four days were taken to complete their life cycle. Cinnamon and Neem oil in cooperated with Surfactant and Kerosene oil could be effectively used as potential chemical agents for control of P. marginatus.
基金Supported by National Key Technology R&D Program of China(2012BAK11B04)
文摘This study was to establish a molecular identification method to distinguish Dermacentor nuttalli(D. nuttalli) and Dermacentor marginatus(D. marginatus),and to study their phylogenetic relationship. The ticks were collected from domestic animals in Xinjiang Uygur Autonomous Region for morphological identification,then their genomic DNAs were isolated for amplifying mitochondrial 16 S rRNA and cytochrome oxidase subunit I gene(COI). The phylogenetic tree was constructed based on the sequencing results of PCR products by employing Mega 5. 0 and Mrbayes 3. 2 for homology analysis. Clustering analysis PCR product for 16 S rRNA from both D. marginatus and D. nuttalli was clustered together with their respective 16 S rRNA sequences previously accessed in GenBank,and that for COI gene as well. These are basically identical with morphological identification results. Our results indicate that morphological identification,combined with molecular markers,would be a simple and accurate method for distinguishing D. nuttalli and D. marginatus.
文摘用磁珠-生物素标记的微卫星探针(ACA)15与白缘缺(Leiobagrus marginatus)基因组酶切片段杂交,捕获含有微卫星序列的DNA片段,连接到T载体中,构建富集微卫星序列的小片段插入文库,再用γ-32P标记的探针进行二次筛选,获得阳性克隆785个,对其中的140个克隆进行了测序,获得完整的微卫星序列132个．用引物设计软件Primer Premier 5．0设计引物64对．对其中的20对引物进行了多态性检测,筛选到稳定扩增的标记13对,用此13对微卫星引物分析了缺属白缘缺(L．marginalus)、拟缘缺(L．marginatoides)(大、小各1种)、黑尾缺(L．nigricauda)共4个群体的遗传多样性,其中10对微卫星引物在种内或种间表现出多态性．
文摘【目的】木瓜秀粉蚧Paracoccus marginatus Williams and Granara de Willink是我国一种重要的新入侵害虫,为筛选出防治木瓜秀粉蚧的高效药剂,明确其对不同杀虫剂的敏感性。【方法】采用浸叶法测定了2龄若虫和雌成虫对15种常用杀虫剂的敏感性。【结果】从15种药剂中筛选出5种对木瓜秀粉蚧毒力较高的杀虫剂,分别为25 g·L^-1高效氯氟氰菊酯乳油、10%虫螨腈悬浮剂、4.5%高效氯氰菊酯乳油、15%哒螨灵水乳剂和15%唑虫酰胺悬浮剂;其中,25 g·L^-1高效氯氟氰菊酯乳油对木瓜秀粉蚧的致死中浓度(LC50)最小,2龄若虫和雌成虫的致死中浓度LC50分别为0.243 0、0.782 1 mg·L^-1,其次为10%虫螨腈悬浮剂、4.5%高效氯氰菊酯乳油和15%哒螨灵水乳剂,15%唑虫酰胺悬浮剂的LC50值最大;木瓜秀粉蚧2龄若虫对药剂的敏感性明显高于雌成虫。【结论】就生物活性测定而言,在木瓜秀粉蚧爆发时,可选用25 g·L^-1高效氯氟氰菊酯乳油、10%虫螨腈悬浮剂、4.5%高效氯氰菊酯乳油、15%哒螨灵水乳剂和15%唑虫酰胺悬浮剂作为当前防治木瓜秀粉蚧的主要防治药剂,可有效遏制入侵害虫木瓜秀粉蚧的危害。
