Bupleuri Radix has been widely used in traditional Chinese medicine.In the current herbal market,the species Bupleurum marginatum Wall.ex DC.is the main source of Bupleuri Radix.Although Bupleuri Radix from the roots ...Bupleuri Radix has been widely used in traditional Chinese medicine.In the current herbal market,the species Bupleurum marginatum Wall.ex DC.is the main source of Bupleuri Radix.Although Bupleuri Radix from the roots of B.marginatum grown wild in the North West of Hubei province has higher quality compared with those from other regions according to the previous investigations,the exhaustive exploitation driven by increasing demand has drastically reduced the wild resource.As a result,germplasm evaluation and quality resource exploration are important for the sustainable utilization and cultivation of B.marginatum.A preliminary study indicated differences in the tissue structure of B.marginatum grown in different areas of North Western Hubei province.In the current study,various tissues of the roots of B.marginatum grown in different areas of North Western Hubei were subjected to laser microdissection and analyzed by microscopy and ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry(UHPLC–Q-TOF-MS).The results show that wild plants from Maqiao Town,Baokang County contain the most saikosaponins distributed mainly in cork,cortex and phloem.This study provides key chemical information for evaluating the quality of B.marginatum roots.展开更多
In China,some species of Bupleurum (family Umbelliferae) have been used as Chinese traditional medicine under the name “Chai-Hu” for the treatment of fevers and influenza.In the present paper,the authors report a st...In China,some species of Bupleurum (family Umbelliferae) have been used as Chinese traditional medicine under the name “Chai-Hu” for the treatment of fevers and influenza.In the present paper,the authors report a study on the investigation and exploitation of new medicinal resources of “Chai-Hu” in Qinghai Province and Xizang (Tibet) Autonomous Region where no one has studied this hitherto.18 species,7 varieties,I form of Bupleurum and a new species (Bupleurum qinghaiense) have been discovered.The taxonomy and preliminary phytochemical studies of the roots of three medicinal plants,B.qinghaiense,B.rnarginatum var.stenophyllum and B.smithii var.parvifolium.are reported in this paper.展开更多
Seventeen compounds were isolated from aerial part of Bupleurum marginatum Wall. ex DC. by column chromatography method, and thirteen compounds were identified by means of physical and spectral data. These compounds w...Seventeen compounds were isolated from aerial part of Bupleurum marginatum Wall. ex DC. by column chromatography method, and thirteen compounds were identified by means of physical and spectral data. These compounds were quercetin (Ⅰ), stigmasta-7, 25-dien-3-ol (Ⅱ), 7-stigmasten-3β-ol (Ⅲ), cholest-7-en-3β-ol (Ⅳ), α-spinasterol (Ⅴ), octacosanoic acid (Ⅵ), β-sitosterol (Ⅶ), daucosterol (Ⅷ), isorhamnetin (Ⅸ), quercetin-3-O-β-D-glucoside (Ⅹ), rutin (Ⅺ), saikochromone A (Ⅻ) and adonitol (). Compound Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ, Ⅵ, Ⅸ, Ⅹ and were firstly isolated from this species.展开更多
基金This work was supported by the Hong Kong Research Grants Council(GRF)(HKBU-263412).
文摘Bupleuri Radix has been widely used in traditional Chinese medicine.In the current herbal market,the species Bupleurum marginatum Wall.ex DC.is the main source of Bupleuri Radix.Although Bupleuri Radix from the roots of B.marginatum grown wild in the North West of Hubei province has higher quality compared with those from other regions according to the previous investigations,the exhaustive exploitation driven by increasing demand has drastically reduced the wild resource.As a result,germplasm evaluation and quality resource exploration are important for the sustainable utilization and cultivation of B.marginatum.A preliminary study indicated differences in the tissue structure of B.marginatum grown in different areas of North Western Hubei province.In the current study,various tissues of the roots of B.marginatum grown in different areas of North Western Hubei were subjected to laser microdissection and analyzed by microscopy and ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry(UHPLC–Q-TOF-MS).The results show that wild plants from Maqiao Town,Baokang County contain the most saikosaponins distributed mainly in cork,cortex and phloem.This study provides key chemical information for evaluating the quality of B.marginatum roots.
文摘In China,some species of Bupleurum (family Umbelliferae) have been used as Chinese traditional medicine under the name “Chai-Hu” for the treatment of fevers and influenza.In the present paper,the authors report a study on the investigation and exploitation of new medicinal resources of “Chai-Hu” in Qinghai Province and Xizang (Tibet) Autonomous Region where no one has studied this hitherto.18 species,7 varieties,I form of Bupleurum and a new species (Bupleurum qinghaiense) have been discovered.The taxonomy and preliminary phytochemical studies of the roots of three medicinal plants,B.qinghaiense,B.rnarginatum var.stenophyllum and B.smithii var.parvifolium.are reported in this paper.
文摘Seventeen compounds were isolated from aerial part of Bupleurum marginatum Wall. ex DC. by column chromatography method, and thirteen compounds were identified by means of physical and spectral data. These compounds were quercetin (Ⅰ), stigmasta-7, 25-dien-3-ol (Ⅱ), 7-stigmasten-3β-ol (Ⅲ), cholest-7-en-3β-ol (Ⅳ), α-spinasterol (Ⅴ), octacosanoic acid (Ⅵ), β-sitosterol (Ⅶ), daucosterol (Ⅷ), isorhamnetin (Ⅸ), quercetin-3-O-β-D-glucoside (Ⅹ), rutin (Ⅺ), saikochromone A (Ⅻ) and adonitol (). Compound Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ, Ⅵ, Ⅸ, Ⅹ and were firstly isolated from this species.
