[Objectives]To establish quality standards for traditional Yi medicine of the Anaphalis margaritacea.[Methods]The original plants,traits,identification and TLC was used to identify L.franchetii Beauv.water content,tot...[Objectives]To establish quality standards for traditional Yi medicine of the Anaphalis margaritacea.[Methods]The original plants,traits,identification and TLC was used to identify L.franchetii Beauv.water content,total ash,acid-insoluble ash and extract were checked according to the Chinese Pharmacopoeia(2020 Edition).The content of 3,5-dicaffeoylquinic acid was determined by HPLC.[Results]The microscopic identification features are obvious,epidermal cells,stomata,non-glandular hairs,annular vessel,conduit wood fiber,pollen grains,starch grains,calcium oxalate clusters were observed,etc.TLC showed clear spots and good resolution.The extract content of acid-insoluble ash aqueous solution in 16 batches of samples was 5.22%-9.33%,6.79%-14.47%,0.39%-3.30%,20.49%-32.93%,respectively.3,5-O-dicafeylquinic acid showed good linear relationships within their own ranges(R^(2)=1.0),whose average recoveries were 99.11%with the RSD of 1.35%,respectively.[Conclusions]The method adopted was reasonable and feasible,and could be used for quality control of A.margaritacea.展开更多
基金Support by National Key R&D Program(2018YFC1706101)Sichuan Science and Technology Plan Project(2021YFS0043)+2 种基金Chinese Medicine(Ethnic Medicine)Standard Promotion Project of Sichuan Drug Administration(510201202102305)2021 Leading Talent Support Program of State Ethnic Affairs CommissionFundamental Research Funds for the Central Universities—Special University-level Innovation Team Project(ZYN2022067)。
文摘[Objectives]To establish quality standards for traditional Yi medicine of the Anaphalis margaritacea.[Methods]The original plants,traits,identification and TLC was used to identify L.franchetii Beauv.water content,total ash,acid-insoluble ash and extract were checked according to the Chinese Pharmacopoeia(2020 Edition).The content of 3,5-dicaffeoylquinic acid was determined by HPLC.[Results]The microscopic identification features are obvious,epidermal cells,stomata,non-glandular hairs,annular vessel,conduit wood fiber,pollen grains,starch grains,calcium oxalate clusters were observed,etc.TLC showed clear spots and good resolution.The extract content of acid-insoluble ash aqueous solution in 16 batches of samples was 5.22%-9.33%,6.79%-14.47%,0.39%-3.30%,20.49%-32.93%,respectively.3,5-O-dicafeylquinic acid showed good linear relationships within their own ranges(R^(2)=1.0),whose average recoveries were 99.11%with the RSD of 1.35%,respectively.[Conclusions]The method adopted was reasonable and feasible,and could be used for quality control of A.margaritacea.
