目的:建立Taq M an-MGB探针Real-tim e荧光PCR快速检测单增李斯特菌技术。方法:在对单增李斯特菌invA序列进行分析、比较基础上,设计一对特异性Taq M an-MGB探针法引物及探针,通过Real-tim ePCR反应条件和反应体系的优化,实现对单增李...目的:建立Taq M an-MGB探针Real-tim e荧光PCR快速检测单增李斯特菌技术。方法:在对单增李斯特菌invA序列进行分析、比较基础上,设计一对特异性Taq M an-MGB探针法引物及探针,通过Real-tim ePCR反应条件和反应体系的优化,实现对单增李斯特菌的快速检测;用克隆到pMD18-T载体上的李斯特菌invA基因阳参片段及不同菌株、食品标本验证方法的特异性和敏感性。结果:方法的灵敏性高,其循环阈值与模板浓度的对数值具有很好的对应关系,最低可检测57个拷贝数,经18 h增菌,可检测低至4.5 cfu/m l的细菌;特异性强,检测6株单增李斯特菌标准株和100株单增李斯特菌样品分离株的PCR循环域值(CT值)均小于25,而90株威氏李斯特菌、英诺克李斯特菌、绵羊李斯特菌以及非李斯特菌PCR循环域值(CT值)大于35;快速,最快1 h可出结果,实际样品20 h可出结果,而常规细菌培养法需要一周以上;对103份速冻米面食品进行检测,13份阳性,与常规方法无显著性差异(P>0.05)。结论:Taq M an-MGB探针的单增李斯特菌Real-tim e荧光PCR检测方法具有特异性强,敏感性高,易操作等优点,可推广应用。展开更多
Man-land relationship research, as the core of geographical research, runs through each development stage of Geography. Based on extensive literature review, this paper systematically generalizes the connotations, res...Man-land relationship research, as the core of geographical research, runs through each development stage of Geography. Based on extensive literature review, this paper systematically generalizes the connotations, research development and contents of man-land relationship in China. （1） It explores the connotations and evolvement rules of man-land rela- tionship in different social development stages in China, and finds that the core role of man-land relationship in geographical research has been strengthened continuously. Changing with times, its connotations have been considerably enriched by sustainable de- velopment and other notions, and so does its theoretical system. （2） It applies the bibliometric method to sketch out the basic research status of man-land relationship in China. Specifically, it quantitatively identifies the funding sources, major research teams and journals for publica- tion. It finds that the funding sources show a diversification trend with national funding being the primary source of research grants. The most competitive research teams are mainly concentrated in the Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences （CAS） and normal universities. Journals sponsored by The Geographical Society of China are most influential in publishing man-land relationship re- search. （3） As resources and environment are the fundamental carriers of man-land rela- tionship, this study focuses further on the research on resource-environmental base of man-land relationship, and finds that the resource-environmental base research in China has gone through an evolution process from single factor perspective research to comprehensive multiple perspective research gradually. Research themes have also experienced similar changes from land, water, energy minerals or other single factor research to comprehensive factor research of resources and environment. Empirical study on national and regional de- velopment strategies is the feature of man-land relationship in China. More emphasis should be put on considering and following the changes in features of ＂man＂ and ＂land＂ and research on the impacts of new factors on man-land relationship in a developing and dynamic manner in the future. Particularly, we should pay more attention to research on the impacts of spa- tio-temporal changes in resource-environment absolute location on modes of man-land in- teraction, and to strengthening interdisciplinary research and systematic research on comprehensive techniques so as to advance the development of application of man-land rela-tionship theories and practices.展开更多
Function allocation is one of the necessary stages in the design course of man-machine systems since appropriate function allocation makes the whole system more effective, reliable and inexpensive. Therefore, our rese...Function allocation is one of the necessary stages in the design course of man-machine systems since appropriate function allocation makes the whole system more effective, reliable and inexpensive. Therefore, our research mainly focuses on the problems of function allocation between man and machine in man-machine systems, analyses each capability advantage of man and machine according to their respective inherent characteristics and makes a comparison between them. In view of highly uncertain characteristics of decision attribute value in the practical process, we introduce the uncertain linguistic multiple attribute decision making （ULMADM） method in the function allocation process. Meanwhile, we also use the uncertain extended weighted arithmetic averaging （UEWAA） method to determine the automation level range of the operator functions. Then, we eventually estab- lish the automation level of man-machine function allocation by using the multi-attribute decision making algorithm, which is combined by UEWAA and uncertain linguistic hybrid aggregation （ULHA） operators. Finally, an example about function allocation is given, that is, fault diagnosis in the cockpit of civil aircraft. The final result of the example demonstrates that the proposed method about function allocation is feasible and effective.展开更多
This paper describes the interaction between deep-seated landslides and man-made structures such as dams, penstocks, viaducts, and tunnels. Selected case studies are reported first with the intent to gain insights int...This paper describes the interaction between deep-seated landslides and man-made structures such as dams, penstocks, viaducts, and tunnels. Selected case studies are reported first with the intent to gain insights into the complexities associated with the interaction of these structures with deep-seated landslides(generally referred to as deep-seated gravity slope deformations, DSGSDs). The main features, which characterize these landslides, are mentioned together with the interaction problems encountered in each case. Given the main objective of this paper, the numerical modeling methods adopted are outlined as means for increase in the understanding of the interaction problems being investigated. With the above in mind, the attention moves to an important and unique case history dealing with the interaction of a large-size twin-tunnel excavated with an earth pressure balance(EPB)tunnel boring machine(TBM) and a deep-seated landslide, which was reactivated due to the stress changes induced by tunnel excavation in landslide shear zone. The geological and geotechnical conditions are described together with the available monitoring data on the landslide movements, based on the advanced and conventional monitoring tools used. Numerical modeling is illustrated as an aid to back-analyze the monitored surface and subsurface deformations and to assist in finding the appropriate engineering solution for putting the tunnel into service and as a follow-up means for future understanding and control of the interaction problems. The simulation is based on a novel time-dependent model representing the landslide behavior.展开更多
A mathematical model of man-machine system is considered.Based on the reference [4],the direction and stability of the Hopf bifurcation are determined using the normal form method and the center manifold theory.Furthe...A mathematical model of man-machine system is considered.Based on the reference [4],the direction and stability of the Hopf bifurcation are determined using the normal form method and the center manifold theory.Furthermore,the existence of Hopf-zero bifurcation is discussed.In the end,some numerical simulations are carried out to illustrate the results found.展开更多
Man-made object detection is of great significance in both military and civil areas, such as search-and-rescue missions at sea, traffic signs recognition during visual navigation, and targets location in a military st...Man-made object detection is of great significance in both military and civil areas, such as search-and-rescue missions at sea, traffic signs recognition during visual navigation, and targets location in a military strike. Contours of man-made objects usually consist of straight lines, corner points, and simple curves. Motivated by this observation, a man-made object detection method is proposed based on complexity evaluation of object contours. After salient contours which keep the crucial information of objects are accurately extracted using an improved mean-shift clustering algorithm, a novel approach is presented to evaluate the complexity of contours. By comparing the entropy values of contours before/after sampling and linear interpolation, it is easy to distinguish between man-made objects and natural ones according to the complexity of their contours.Experimental results show that the presented method can effectively detect man-made objects when compared to the existing ones.展开更多
A system that allows computer interaction by disabled people with very low mobility and who cannot use the standard procedure based on keyboard and mouse is presented. The development device uses the patient’s volunt...A system that allows computer interaction by disabled people with very low mobility and who cannot use the standard procedure based on keyboard and mouse is presented. The development device uses the patient’s voluntary biomechanical signals, specifically, winks—which constitute an ability that generally remains in this kind of patients—, as interface to control the computer. A prototype based on robust and low-cost elements has been built and its performance has been validated through real trials by 16 users without previous training. The system can be optimized after a learning period in order to be adapted to every user. Also, good results were obtained in a subjective satisfaction survey that was completed by the users after carrying out the test trials.展开更多
A post- colonial reading of the story of Man- Man from Miguel Street by V.S. Nailpaul in the light of Homi K.Bhabha's views about mimicry reveals that to some extent mimicry in Naipaul's post-colonial writing ...A post- colonial reading of the story of Man- Man from Miguel Street by V.S. Nailpaul in the light of Homi K.Bhabha's views about mimicry reveals that to some extent mimicry in Naipaul's post-colonial writing reflects the double edged effect of mimicry discussed by Bhabha, but is more associated with the hopelessness and predicament suffered by the once colonized than the resistance to the authority of the colonizer.展开更多
文摘目的:建立Taq M an-MGB探针Real-tim e荧光PCR快速检测单增李斯特菌技术。方法:在对单增李斯特菌invA序列进行分析、比较基础上,设计一对特异性Taq M an-MGB探针法引物及探针,通过Real-tim ePCR反应条件和反应体系的优化,实现对单增李斯特菌的快速检测;用克隆到pMD18-T载体上的李斯特菌invA基因阳参片段及不同菌株、食品标本验证方法的特异性和敏感性。结果:方法的灵敏性高,其循环阈值与模板浓度的对数值具有很好的对应关系,最低可检测57个拷贝数,经18 h增菌,可检测低至4.5 cfu/m l的细菌;特异性强,检测6株单增李斯特菌标准株和100株单增李斯特菌样品分离株的PCR循环域值(CT值)均小于25,而90株威氏李斯特菌、英诺克李斯特菌、绵羊李斯特菌以及非李斯特菌PCR循环域值(CT值)大于35;快速,最快1 h可出结果,实际样品20 h可出结果,而常规细菌培养法需要一周以上;对103份速冻米面食品进行检测,13份阳性,与常规方法无显著性差异(P>0.05)。结论:Taq M an-MGB探针的单增李斯特菌Real-tim e荧光PCR检测方法具有特异性强,敏感性高,易操作等优点,可推广应用。
基金National Natural Science Foundation of China, No.41430636, No.41371141, No.41590841
文摘Man-land relationship research, as the core of geographical research, runs through each development stage of Geography. Based on extensive literature review, this paper systematically generalizes the connotations, research development and contents of man-land relationship in China. （1） It explores the connotations and evolvement rules of man-land rela- tionship in different social development stages in China, and finds that the core role of man-land relationship in geographical research has been strengthened continuously. Changing with times, its connotations have been considerably enriched by sustainable de- velopment and other notions, and so does its theoretical system. （2） It applies the bibliometric method to sketch out the basic research status of man-land relationship in China. Specifically, it quantitatively identifies the funding sources, major research teams and journals for publica- tion. It finds that the funding sources show a diversification trend with national funding being the primary source of research grants. The most competitive research teams are mainly concentrated in the Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences （CAS） and normal universities. Journals sponsored by The Geographical Society of China are most influential in publishing man-land relationship re- search. （3） As resources and environment are the fundamental carriers of man-land rela- tionship, this study focuses further on the research on resource-environmental base of man-land relationship, and finds that the resource-environmental base research in China has gone through an evolution process from single factor perspective research to comprehensive multiple perspective research gradually. Research themes have also experienced similar changes from land, water, energy minerals or other single factor research to comprehensive factor research of resources and environment. Empirical study on national and regional de- velopment strategies is the feature of man-land relationship in China. More emphasis should be put on considering and following the changes in features of ＂man＂ and ＂land＂ and research on the impacts of new factors on man-land relationship in a developing and dynamic manner in the future. Particularly, we should pay more attention to research on the impacts of spa- tio-temporal changes in resource-environment absolute location on modes of man-land in- teraction, and to strengthening interdisciplinary research and systematic research on comprehensive techniques so as to advance the development of application of man-land rela-tionship theories and practices.
文摘Function allocation is one of the necessary stages in the design course of man-machine systems since appropriate function allocation makes the whole system more effective, reliable and inexpensive. Therefore, our research mainly focuses on the problems of function allocation between man and machine in man-machine systems, analyses each capability advantage of man and machine according to their respective inherent characteristics and makes a comparison between them. In view of highly uncertain characteristics of decision attribute value in the practical process, we introduce the uncertain linguistic multiple attribute decision making （ULMADM） method in the function allocation process. Meanwhile, we also use the uncertain extended weighted arithmetic averaging （UEWAA） method to determine the automation level range of the operator functions. Then, we eventually estab- lish the automation level of man-machine function allocation by using the multi-attribute decision making algorithm, which is combined by UEWAA and uncertain linguistic hybrid aggregation （ULHA） operators. Finally, an example about function allocation is given, that is, fault diagnosis in the cockpit of civil aircraft. The final result of the example demonstrates that the proposed method about function allocation is feasible and effective.
基金support of Spea Ingegneria Europea SpA and Società Autostrade per l’Italia SpA
文摘This paper describes the interaction between deep-seated landslides and man-made structures such as dams, penstocks, viaducts, and tunnels. Selected case studies are reported first with the intent to gain insights into the complexities associated with the interaction of these structures with deep-seated landslides(generally referred to as deep-seated gravity slope deformations, DSGSDs). The main features, which characterize these landslides, are mentioned together with the interaction problems encountered in each case. Given the main objective of this paper, the numerical modeling methods adopted are outlined as means for increase in the understanding of the interaction problems being investigated. With the above in mind, the attention moves to an important and unique case history dealing with the interaction of a large-size twin-tunnel excavated with an earth pressure balance(EPB)tunnel boring machine(TBM) and a deep-seated landslide, which was reactivated due to the stress changes induced by tunnel excavation in landslide shear zone. The geological and geotechnical conditions are described together with the available monitoring data on the landslide movements, based on the advanced and conventional monitoring tools used. Numerical modeling is illustrated as an aid to back-analyze the monitored surface and subsurface deformations and to assist in finding the appropriate engineering solution for putting the tunnel into service and as a follow-up means for future understanding and control of the interaction problems. The simulation is based on a novel time-dependent model representing the landslide behavior.
基金Foundation item： Supported bY the Natural Science Foundation of Ningxia（NZ09204） Supported by the Youth Foundation of Ningxia Teacher＇s Universlty（QN2010002）
文摘A mathematical model of man-machine system is considered.Based on the reference [4],the direction and stability of the Hopf bifurcation are determined using the normal form method and the center manifold theory.Furthermore,the existence of Hopf-zero bifurcation is discussed.In the end,some numerical simulations are carried out to illustrate the results found.
基金co-supported by the National Natural Science Foundation of China (61473148)the Funding of Jiangsu Innovation Program for Graduate Education (No. KYLX16_0337)
文摘Man-made object detection is of great significance in both military and civil areas, such as search-and-rescue missions at sea, traffic signs recognition during visual navigation, and targets location in a military strike. Contours of man-made objects usually consist of straight lines, corner points, and simple curves. Motivated by this observation, a man-made object detection method is proposed based on complexity evaluation of object contours. After salient contours which keep the crucial information of objects are accurately extracted using an improved mean-shift clustering algorithm, a novel approach is presented to evaluate the complexity of contours. By comparing the entropy values of contours before/after sampling and linear interpolation, it is easy to distinguish between man-made objects and natural ones according to the complexity of their contours.Experimental results show that the presented method can effectively detect man-made objects when compared to the existing ones.
文摘A system that allows computer interaction by disabled people with very low mobility and who cannot use the standard procedure based on keyboard and mouse is presented. The development device uses the patient’s voluntary biomechanical signals, specifically, winks—which constitute an ability that generally remains in this kind of patients—, as interface to control the computer. A prototype based on robust and low-cost elements has been built and its performance has been validated through real trials by 16 users without previous training. The system can be optimized after a learning period in order to be adapted to every user. Also, good results were obtained in a subjective satisfaction survey that was completed by the users after carrying out the test trials.
文摘A post- colonial reading of the story of Man- Man from Miguel Street by V.S. Nailpaul in the light of Homi K.Bhabha's views about mimicry reveals that to some extent mimicry in Naipaul's post-colonial writing reflects the double edged effect of mimicry discussed by Bhabha, but is more associated with the hopelessness and predicament suffered by the once colonized than the resistance to the authority of the colonizer.
