The haploid-inducing line Stock 6 was used to produce haploid maize and expected to obtain maize haploid plants successfully.The detailed meiotic studies on selected haploid maize(n=x=10)were conducted.Cytogenetic ana...The haploid-inducing line Stock 6 was used to produce haploid maize and expected to obtain maize haploid plants successfully.The detailed meiotic studies on selected haploid maize(n=x=10)were conducted.Cytogenetic analysis revealed a high frequency of meiotic abnormality occurred in both meiosisⅠand meiosisⅡ.During the prophaseⅠ,univalents were common configurations,and there were bivalents or trivalents in some pollen mother cells,however,a few cells containing five bivalents were also observed.After prophaseⅠ,chromosomes did not congregate in a single metaphase plate but they were scattered in the cytoplasm.At anaphaseⅠ,the chromosome distribution was highly irregular with almost all possible combinations.In some cells,chromosomes were grouped into the three or four masses and several spindles appeared.At the tetrad stage of meiosisⅡ,eytokinesis splitting abnormality occurred,and a variety of diad,triad,tetrad,pentad,hexad,as well as decury microspores were easily observed.As a consequence of abnormalities of the two meiotic stages,various microspores and the pollen were almost completely sterile.grains with different size were formed,and its pollen grains were almost completely sterile.展开更多
基金supported by the National 973 Program of China(2007CB108900)the National Natural Science Foundation of China(30671308)+2 种基金SichuanYouth Science and Technology Fund,China(2007Q14-032)Ministry of Education Innovation Team Development Plan,China(IRT0453)Sichuan Forage Grass Breeding Project,China
文摘The haploid-inducing line Stock 6 was used to produce haploid maize and expected to obtain maize haploid plants successfully.The detailed meiotic studies on selected haploid maize(n=x=10)were conducted.Cytogenetic analysis revealed a high frequency of meiotic abnormality occurred in both meiosisⅠand meiosisⅡ.During the prophaseⅠ,univalents were common configurations,and there were bivalents or trivalents in some pollen mother cells,however,a few cells containing five bivalents were also observed.After prophaseⅠ,chromosomes did not congregate in a single metaphase plate but they were scattered in the cytoplasm.At anaphaseⅠ,the chromosome distribution was highly irregular with almost all possible combinations.In some cells,chromosomes were grouped into the three or four masses and several spindles appeared.At the tetrad stage of meiosisⅡ,eytokinesis splitting abnormality occurred,and a variety of diad,triad,tetrad,pentad,hexad,as well as decury microspores were easily observed.As a consequence of abnormalities of the two meiotic stages,various microspores and the pollen were almost completely sterile.grains with different size were formed,and its pollen grains were almost completely sterile.
