为探究大豆基于株高抗旱系数(drought resistance coefficient based on plant height,DCPH)和主茎节数抗旱系数(drought resistance coefficient based on number of main stem nodes,DCNS)的抗性遗传基础,本研究选取由113份大豆品种(...为探究大豆基于株高抗旱系数(drought resistance coefficient based on plant height,DCPH)和主茎节数抗旱系数(drought resistance coefficient based on number of main stem nodes,DCNS)的抗性遗传基础,本研究选取由113份大豆品种(系)组成的自然群体作为研究材料,在全生育期干旱胁迫和正常供水条件下测定了株高和主茎节数,分别计算两种类型的抗旱系数,并利用全基因组关联分析技术(Genome-Wide Association Study,GWAS),挖掘与大豆株高和主茎节数抗旱性相关的基因和位点。结果显示:利用1882531个SNP标记进行GWAS分析,DCPH显著关联的位点全部位于9号染色体上,而DCNS显著关联的位点全部位于6号染色体上。进一步分析确定了DCPH和DCNS的候选基因区间,分别筛选出41个与DCPH相关的候选基因和15个与DCNS相关的候选基因。这些基因可能参与大豆生长发育的调控、激素信号传导、细胞分裂和生长等过程。本研究不仅为深入解析大豆抗旱性的分子机制提供了重要线索,还为培育抗旱性强的大豆品种提供了宝贵的基因资源。展开更多
As the ecologically important recipient channels for riverine ecosystems, tributaries provide unique microhabitats for microorganisms, among which zooplankton constitutes the most important heterotrophic organisms. In...As the ecologically important recipient channels for riverine ecosystems, tributaries provide unique microhabitats for microorganisms, among which zooplankton constitutes the most important heterotrophic organisms. In particular, the reduced water velocity caused by dams is more favorable for zooplankton development;therefore, dammed rivers are expected to support extremely diverse and abundant zooplankton communities and notably different spatiotemporal distribution patterns. So far,however, only very few molecular studies support these assumptions. Using high-throughput sequencing, a high number of 350 operational taxonomic units(OTUs;97% cutoff) were retrieved from 30 samples collected in the Xiangxi River, the nearest large tributary upstream of the Three Gorges Dam. Zooplankton did not show significant spatial distribution in the channel. Instead,the community structures varied significantly over sampling dates, corroborating the seasonal patterns found in lakes and ponds in the subtropical zone. As expected, the community compositions were deterministically governed by environmental filtering processes(phylogenetic clustering), in which water velocity appeared to be much less important than other investigated environmental factors. Moreover, most of the detected phylotypes(OTUs) had a relatively high(>90%) sequence similarity to previously deposited sequences, suggesting a mediocre degree of genetic novelty within the zooplankton communities in the Xiangxi River.展开更多
Objective: The aim was to show that Minimally Invasive total arterial revascularization for left main stem coronary artery disease, via a left anterior Mini thoracotomy using bilateral internal thoracic arteries is fe...Objective: The aim was to show that Minimally Invasive total arterial revascularization for left main stem coronary artery disease, via a left anterior Mini thoracotomy using bilateral internal thoracic arteries is feasible, reproducible and safe. Further, there has been no exclusive data or experience with minimally invasive coronary artery bypass grafting in left main stem disease. Methods: From April 2019 to March 2024, 41 patients with left main stem stenosis, left main equivalent disease and unprotected left main with triple vessel disease underwent off pump minimally invasive multivessel coronary artery bypass grafting using either in situ pedicled Bilateral Internal Thoracic arteries or Left and Right Internal Thoracic artery Y composite conduits at three centers. Bilateral Internal Thoracic arteries were harvested under direct vision. All patients had an Intra-Aortic Balloon Pump inserted via the femoral artery prior to induction of anesthesia, to prevent any hemodynamic instability, arrhythmias, and was removed following completion of the procedure in the operating room without any complications. Efficacy and outcomes were evaluated by i) Primary (MACCE)-Major Adverse Cardiac and Cardiovascular events and ii) Secondary outcome measures including total length of stay, return to full physical activity and quality of life. Mean follow-up was 1.4 years (Maximum was 2.5 years). Results: 41 patients with left main stem coronary artery stenosis, underwent total arterial revascularization using bilateral internal thoracic arteries. Left main stem stenosis was present in 29 patients, Unprotected left main stem stenosis with triple vessel disease in 7 and left main equivalence in 5 patients. In this cohort, 29 patients with only left main stem stenosis had 2 grafts each, 7 patients with left main and triple vessel disease had 3 grafts and 5 patients with left main equivalent disease had 2 grafts respectively. The average number of grafts was 2.2. One patient was converted to open sternotomy as an emergency because of hemodynamic instability and myocardial revascularization was done on cardiopulmonary bypass (2.2%). The average hospital stay was 3.7 days. Ejection fraction was 45% ± 5%. There was one mortality (2.2%) but no major morbidity. The average ICU and hospital stay was 24 ± 4 hours and 3.7 days. All patients were free from major adverse cardiac and cerebrovascular events at follow-up. Conclusions: Multivessel total arterial revascularization using left and right internal thoracic arteries, was performed via a left anterior Mini thoracotomy on patients with left main stem disease and showed that it was safe, reproducible and will help extend the armamentarium of the surgeon in minimally invasive Coronary artery bypass grafting. Concomitantly it helped enhance the potential for shorter hospital stay, increased survival, decreased morbidity, and earlier return to full activity. Furthermore, the safety, efficacy, and outcomes of minimally invasive coronary artery bypass grafting in this high-risk group evaluated by primary and secondary outcome measures have been good in this study.展开更多
选用美棉33B和泗棉3号两个叶色深浅差异明显的品种,设置氮素水平(0、120、240、360和480 kg hm-2纯氮)试验,研究棉花主茎叶SPAD值的叶位分布,对棉花进行氮素营养诊断。结果表明,棉花主茎顶部4张定型叶片的SPAD值对施氮水平反应的敏感程...选用美棉33B和泗棉3号两个叶色深浅差异明显的品种,设置氮素水平(0、120、240、360和480 kg hm-2纯氮)试验,研究棉花主茎叶SPAD值的叶位分布,对棉花进行氮素营养诊断。结果表明,棉花主茎顶部4张定型叶片的SPAD值对施氮水平反应的敏感程度存在显著差异,随施氮量增加,棉花主茎顶1、2、3、4定型叶SPAD值的大小位次发生明显变化,其中以顶1定型叶对施氮量增加的响应最为敏感,顶4定型叶最为迟钝。利用顶1定型叶与顶4定型叶SPAD值的相对差值,可较精确地诊断棉花的氮素营养水平,并能克服品种和生育时期不同的影响,是一种具有普适性的棉花氮素营养诊断指标。展开更多
文摘为探究大豆基于株高抗旱系数(drought resistance coefficient based on plant height,DCPH)和主茎节数抗旱系数(drought resistance coefficient based on number of main stem nodes,DCNS)的抗性遗传基础,本研究选取由113份大豆品种(系)组成的自然群体作为研究材料,在全生育期干旱胁迫和正常供水条件下测定了株高和主茎节数,分别计算两种类型的抗旱系数,并利用全基因组关联分析技术(Genome-Wide Association Study,GWAS),挖掘与大豆株高和主茎节数抗旱性相关的基因和位点。结果显示:利用1882531个SNP标记进行GWAS分析,DCPH显著关联的位点全部位于9号染色体上,而DCNS显著关联的位点全部位于6号染色体上。进一步分析确定了DCPH和DCNS的候选基因区间,分别筛选出41个与DCPH相关的候选基因和15个与DCNS相关的候选基因。这些基因可能参与大豆生长发育的调控、激素信号传导、细胞分裂和生长等过程。本研究不仅为深入解析大豆抗旱性的分子机制提供了重要线索,还为培育抗旱性强的大豆品种提供了宝贵的基因资源。
基金supported by the National Natural Science Foundation of China (31772433, 31801984)Biodiversity survey of aquatic organisms in Three Gorges Reservoir area and the illustrated handbook compilation (2014FY120200)
文摘As the ecologically important recipient channels for riverine ecosystems, tributaries provide unique microhabitats for microorganisms, among which zooplankton constitutes the most important heterotrophic organisms. In particular, the reduced water velocity caused by dams is more favorable for zooplankton development;therefore, dammed rivers are expected to support extremely diverse and abundant zooplankton communities and notably different spatiotemporal distribution patterns. So far,however, only very few molecular studies support these assumptions. Using high-throughput sequencing, a high number of 350 operational taxonomic units(OTUs;97% cutoff) were retrieved from 30 samples collected in the Xiangxi River, the nearest large tributary upstream of the Three Gorges Dam. Zooplankton did not show significant spatial distribution in the channel. Instead,the community structures varied significantly over sampling dates, corroborating the seasonal patterns found in lakes and ponds in the subtropical zone. As expected, the community compositions were deterministically governed by environmental filtering processes(phylogenetic clustering), in which water velocity appeared to be much less important than other investigated environmental factors. Moreover, most of the detected phylotypes(OTUs) had a relatively high(>90%) sequence similarity to previously deposited sequences, suggesting a mediocre degree of genetic novelty within the zooplankton communities in the Xiangxi River.
文摘Objective: The aim was to show that Minimally Invasive total arterial revascularization for left main stem coronary artery disease, via a left anterior Mini thoracotomy using bilateral internal thoracic arteries is feasible, reproducible and safe. Further, there has been no exclusive data or experience with minimally invasive coronary artery bypass grafting in left main stem disease. Methods: From April 2019 to March 2024, 41 patients with left main stem stenosis, left main equivalent disease and unprotected left main with triple vessel disease underwent off pump minimally invasive multivessel coronary artery bypass grafting using either in situ pedicled Bilateral Internal Thoracic arteries or Left and Right Internal Thoracic artery Y composite conduits at three centers. Bilateral Internal Thoracic arteries were harvested under direct vision. All patients had an Intra-Aortic Balloon Pump inserted via the femoral artery prior to induction of anesthesia, to prevent any hemodynamic instability, arrhythmias, and was removed following completion of the procedure in the operating room without any complications. Efficacy and outcomes were evaluated by i) Primary (MACCE)-Major Adverse Cardiac and Cardiovascular events and ii) Secondary outcome measures including total length of stay, return to full physical activity and quality of life. Mean follow-up was 1.4 years (Maximum was 2.5 years). Results: 41 patients with left main stem coronary artery stenosis, underwent total arterial revascularization using bilateral internal thoracic arteries. Left main stem stenosis was present in 29 patients, Unprotected left main stem stenosis with triple vessel disease in 7 and left main equivalence in 5 patients. In this cohort, 29 patients with only left main stem stenosis had 2 grafts each, 7 patients with left main and triple vessel disease had 3 grafts and 5 patients with left main equivalent disease had 2 grafts respectively. The average number of grafts was 2.2. One patient was converted to open sternotomy as an emergency because of hemodynamic instability and myocardial revascularization was done on cardiopulmonary bypass (2.2%). The average hospital stay was 3.7 days. Ejection fraction was 45% ± 5%. There was one mortality (2.2%) but no major morbidity. The average ICU and hospital stay was 24 ± 4 hours and 3.7 days. All patients were free from major adverse cardiac and cerebrovascular events at follow-up. Conclusions: Multivessel total arterial revascularization using left and right internal thoracic arteries, was performed via a left anterior Mini thoracotomy on patients with left main stem disease and showed that it was safe, reproducible and will help extend the armamentarium of the surgeon in minimally invasive Coronary artery bypass grafting. Concomitantly it helped enhance the potential for shorter hospital stay, increased survival, decreased morbidity, and earlier return to full activity. Furthermore, the safety, efficacy, and outcomes of minimally invasive coronary artery bypass grafting in this high-risk group evaluated by primary and secondary outcome measures have been good in this study.
文摘选用美棉33B和泗棉3号两个叶色深浅差异明显的品种,设置氮素水平(0、120、240、360和480 kg hm-2纯氮)试验,研究棉花主茎叶SPAD值的叶位分布,对棉花进行氮素营养诊断。结果表明,棉花主茎顶部4张定型叶片的SPAD值对施氮水平反应的敏感程度存在显著差异,随施氮量增加,棉花主茎顶1、2、3、4定型叶SPAD值的大小位次发生明显变化,其中以顶1定型叶对施氮量增加的响应最为敏感,顶4定型叶最为迟钝。利用顶1定型叶与顶4定型叶SPAD值的相对差值,可较精确地诊断棉花的氮素营养水平,并能克服品种和生育时期不同的影响,是一种具有普适性的棉花氮素营养诊断指标。
