Objective:To investigate the therapeutic effects of methanol extract of Citrus macroptera Montr,fruit inα-amylase inhibitory activity(in vitro)and hypoglycemic activity in normal and glucose induced hyperglycemic rat...Objective:To investigate the therapeutic effects of methanol extract of Citrus macroptera Montr,fruit inα-amylase inhibitory activity(in vitro)and hypoglycemic activity in normal and glucose induced hyperglycemic rats(in vivo).Methods:Fruits of Citrus macroptera without rind was extracted with pure methanol following cold extraction and tested for presence of phytochemical constituents,α-amylase inhibitory activity,and hypoglycemic effect in normal rats and glucose induced hyperglycemic rats.Results:Presence of saponin,steroid and terpenoid were identified in the extract.The results showed that fruit extract had moderateα-amylase inhibitory activity[IC_(50)value=(3.638±0.190)mg/mL]as compared to acarbose.Moreover at 500 mg/kg and 1000 mg/kg doses fruit extract significantly(P<0.05 and P<0.01 respectively)reduced fasting blood glucose level in normal rats as compared to glibenclamide(5 mg/kg).In oral glucose tolerance test,500 mg/kg dose significantly reduced blood glucose level(P<0.05)at 2 h but 1000 mg/kg dose significantly reduced blood glucose level at 2 h and 3 h(P<0.05 and P<0.01 respectively)whereas glibenclamide(5 mg/kg)significantly reduced glucose level at every hour after administration.Overall time effect is also considered extremely significant with F value=23.83 and P value=0.0001 in oral glucose tolerance test.Conclusion:These findings suggest that the plant may be a potential source for the development of new oral hypoglycemic agent.展开更多
Objective:To analyze the phytochemicals that are present in two medicinal plants which are used for the treatment of respiratory tract infections by gas chromatography-mass spectrometer.Methods:The plant leaves were e...Objective:To analyze the phytochemicals that are present in two medicinal plants which are used for the treatment of respiratory tract infections by gas chromatography-mass spectrometer.Methods:The plant leaves were extracted with n-hexane and methanol separately.Both extracts were analyzed for present phytochemicals using the method described by Harborne,1985 while only methanol extracts were subjected to gas chromatography-mass spectrometer analysis.Results:Phytochemical screening of the methanolic extracts of Terminalia macroptera(T.macroptera)revealed the presence of glycosides,tannins,flavonoids,saponins and steroids while that of Dioclea reflexa(D.reflexa)showed the presence of flavonoids,saponins and steroids.The n-hexane extracts were devoid of the screened phytochemicals.Twelve and twenty-five compounds were identified in the leaves of T.macroptera and D.reflexa respectively.These compounds were fatty acids,fatty acid esters,other esters,heterocyclics and phenolics.The most abundant compound in T.macroptera was benzenetriol(53.30%)while the predominant compounds in D.reflexa were dodecanoic acid,methyl ester(15.31%),5,5,8a-trimethyl-3,5,6,7,8,8a-hexahydro-2H-chromene(9.73%),10-octadecenoic acid,methyl ester and 2-hexadecanoic acid,methyl ester(8.95%).Benzofuran,2,3-dihydro,3,7,11,15-tetramethyl-2-hexadecen-1-ol and hexadecanoic acid,methyl ester were common in both plant extracts.The antimicrobial properties of the leaves of these plants could be responsible for their use in the treatment of respiratory tract infections.Conclusions:Some of the identified phytochemicals in the plant leaves are responsible for its use in the treatment of respiratory tract infections.展开更多
基金Supported by Laboratory of Natural Products Research.Jahangirnagar University,Dhaka,Bangladesh
文摘Objective:To investigate the therapeutic effects of methanol extract of Citrus macroptera Montr,fruit inα-amylase inhibitory activity(in vitro)and hypoglycemic activity in normal and glucose induced hyperglycemic rats(in vivo).Methods:Fruits of Citrus macroptera without rind was extracted with pure methanol following cold extraction and tested for presence of phytochemical constituents,α-amylase inhibitory activity,and hypoglycemic effect in normal rats and glucose induced hyperglycemic rats.Results:Presence of saponin,steroid and terpenoid were identified in the extract.The results showed that fruit extract had moderateα-amylase inhibitory activity[IC_(50)value=(3.638±0.190)mg/mL]as compared to acarbose.Moreover at 500 mg/kg and 1000 mg/kg doses fruit extract significantly(P<0.05 and P<0.01 respectively)reduced fasting blood glucose level in normal rats as compared to glibenclamide(5 mg/kg).In oral glucose tolerance test,500 mg/kg dose significantly reduced blood glucose level(P<0.05)at 2 h but 1000 mg/kg dose significantly reduced blood glucose level at 2 h and 3 h(P<0.05 and P<0.01 respectively)whereas glibenclamide(5 mg/kg)significantly reduced glucose level at every hour after administration.Overall time effect is also considered extremely significant with F value=23.83 and P value=0.0001 in oral glucose tolerance test.Conclusion:These findings suggest that the plant may be a potential source for the development of new oral hypoglycemic agent.
文摘Objective:To analyze the phytochemicals that are present in two medicinal plants which are used for the treatment of respiratory tract infections by gas chromatography-mass spectrometer.Methods:The plant leaves were extracted with n-hexane and methanol separately.Both extracts were analyzed for present phytochemicals using the method described by Harborne,1985 while only methanol extracts were subjected to gas chromatography-mass spectrometer analysis.Results:Phytochemical screening of the methanolic extracts of Terminalia macroptera(T.macroptera)revealed the presence of glycosides,tannins,flavonoids,saponins and steroids while that of Dioclea reflexa(D.reflexa)showed the presence of flavonoids,saponins and steroids.The n-hexane extracts were devoid of the screened phytochemicals.Twelve and twenty-five compounds were identified in the leaves of T.macroptera and D.reflexa respectively.These compounds were fatty acids,fatty acid esters,other esters,heterocyclics and phenolics.The most abundant compound in T.macroptera was benzenetriol(53.30%)while the predominant compounds in D.reflexa were dodecanoic acid,methyl ester(15.31%),5,5,8a-trimethyl-3,5,6,7,8,8a-hexahydro-2H-chromene(9.73%),10-octadecenoic acid,methyl ester and 2-hexadecanoic acid,methyl ester(8.95%).Benzofuran,2,3-dihydro,3,7,11,15-tetramethyl-2-hexadecen-1-ol and hexadecanoic acid,methyl ester were common in both plant extracts.The antimicrobial properties of the leaves of these plants could be responsible for their use in the treatment of respiratory tract infections.Conclusions:Some of the identified phytochemicals in the plant leaves are responsible for its use in the treatment of respiratory tract infections.
