应用光学显微镜、显微光度计和定量细胞化学技术研究了一种游仆虫无性生殖周期中大核及其 DNA 含量的变化特征,获得如下结果:(1)游仆虫细胞周期分成 G_1期、S 期和 D 期,各个时期在整个细胞周期中所占的时间速率分别为36%、59%和5%。(2...应用光学显微镜、显微光度计和定量细胞化学技术研究了一种游仆虫无性生殖周期中大核及其 DNA 含量的变化特征,获得如下结果:(1)游仆虫细胞周期分成 G_1期、S 期和 D 期,各个时期在整个细胞周期中所占的时间速率分别为36%、59%和5%。(2)游仆虫细胞周期中,G_1期大核迅速增大,但此时大核无 DNA 合成现象,DNA 分布密度随大核增大而降低;S 期大核前期增大,此后变小,大核 DNA 含量连续增加,大核 DNA 分布密度也随着增高。8期结束后,大核 DNA 含量相当于 G_1大核的两倍;D期大核又显著增大,DNA 分布密度稍高于 G_1期大核的水平。(3)由游仆虫孚尔根反应大核的形态,大核 DNA 三维吸收图像和显微光度术定量分析数据表明,游仆虫大核 DNA 含量的变化仅发生在 S 期,S 期大核中 DNA 合成与大核改组带相联系。展开更多
Right before the excystment of an Euplotes encysti-cus sawtooth-like folds appeared among the pellicle plas- malemma, the inner and outer alveolar membranes were still sticking together, and were not distinguishable. ...Right before the excystment of an Euplotes encysti-cus sawtooth-like folds appeared among the pellicle plas- malemma, the inner and outer alveolar membranes were still sticking together, and were not distinguishable. Mi- crotubular layers already formed at the sites beneath the dorsal cortical pellicle corresponding to vegetative cells,but they still proceed to be organized on the ventral struc-tures. Cristae, highly-tangled with tubular-type struc-tures, appeared on the mitochondria, and were morpho-logically similar to that of vegetative cells. In the cor-tical ciliatures, such as ciliary shafts, kinetosomes, sur-rounding fibrillar cirral baskets, and attached structures of ciliatures, etc., they are different from those in rest- ing cysts which are degenerated or lost. All the cilia- ture microtubules of ciliary shafts are of the 9+2 pattern,but the microtubule-like structure aggregates at triplet - microtubule centers of many kinetosmes, are still under various stages of differentiation. Microtubules beneath the kinetosomal rows are of a developmentally elongated stage; crowded chromatins of various shapes and sizes are found in macronucleus, but there are no nuclear pores (formed by nuclear membrane as in resting cysts) on the nuclear membrane where these chromatins attached.展开更多
文摘应用光学显微镜、显微光度计和定量细胞化学技术研究了一种游仆虫无性生殖周期中大核及其 DNA 含量的变化特征,获得如下结果:(1)游仆虫细胞周期分成 G_1期、S 期和 D 期,各个时期在整个细胞周期中所占的时间速率分别为36%、59%和5%。(2)游仆虫细胞周期中,G_1期大核迅速增大,但此时大核无 DNA 合成现象,DNA 分布密度随大核增大而降低;S 期大核前期增大,此后变小,大核 DNA 含量连续增加,大核 DNA 分布密度也随着增高。8期结束后,大核 DNA 含量相当于 G_1大核的两倍;D期大核又显著增大,DNA 分布密度稍高于 G_1期大核的水平。(3)由游仆虫孚尔根反应大核的形态,大核 DNA 三维吸收图像和显微光度术定量分析数据表明,游仆虫大核 DNA 含量的变化仅发生在 S 期,S 期大核中 DNA 合成与大核改组带相联系。
文摘Right before the excystment of an Euplotes encysti-cus sawtooth-like folds appeared among the pellicle plas- malemma, the inner and outer alveolar membranes were still sticking together, and were not distinguishable. Mi- crotubular layers already formed at the sites beneath the dorsal cortical pellicle corresponding to vegetative cells,but they still proceed to be organized on the ventral struc-tures. Cristae, highly-tangled with tubular-type struc-tures, appeared on the mitochondria, and were morpho-logically similar to that of vegetative cells. In the cor-tical ciliatures, such as ciliary shafts, kinetosomes, sur-rounding fibrillar cirral baskets, and attached structures of ciliatures, etc., they are different from those in rest- ing cysts which are degenerated or lost. All the cilia- ture microtubules of ciliary shafts are of the 9+2 pattern,but the microtubule-like structure aggregates at triplet - microtubule centers of many kinetosmes, are still under various stages of differentiation. Microtubules beneath the kinetosomal rows are of a developmentally elongated stage; crowded chromatins of various shapes and sizes are found in macronucleus, but there are no nuclear pores (formed by nuclear membrane as in resting cysts) on the nuclear membrane where these chromatins attached.
