Objective: To verify that Proliverenol has a potential ability in protecting cells from ethanol-induced hepatotoxicity.Methods: Activity of Proliverenol against ethanol-induced apoptosis was evaluated at m RNA and pro...Objective: To verify that Proliverenol has a potential ability in protecting cells from ethanol-induced hepatotoxicity.Methods: Activity of Proliverenol against ethanol-induced apoptosis was evaluated at m RNA and protein levels in Hep G2 cell exposed to Proliverenol for 1 and 3 h.Results: Proliverenol conferred hepatoprotective activity through increasing cell survival up to 53%–69% via up-regulation of APEX1 DNA repair enzyme for 3.0–4.7 fold and down-regulating of nuclear factor-kB, tumor necrosis factora and caspase-8 expression,allowing them to prevent 4.5–6.9 fold of alanine aminotransferase(ALT) leakage in Hep G2 cells. Our finding revealed that Proliverenol repressed expression of ALT, which is significantly important as possible alternative mechanism for increased blood transaminase activities. In addition, the result also showed that caspase-8 pathway seemed to be involved in the molecular pathway rather than directly inducing mitochondrial damage.Conclusions: The data support our hypothesis that Proliverenol has a potential ability in protecting cells from ethanol-induced hepatotoxicity. We propose that Proliverenol provides hepatoprotective activity through up-regulating expression of APEX1 that repress DNA fragmentation, and down-regulating expression of nuclear factor-kB, tumor necrosis factora and caspase-8, which therefore repress ALT leakage and its expression.展开更多
J. curcas and J. macrocarpa are useful for restoring degraded areas and their seeds contain oils for biodiesel production. The aim of the work was to determine the reserve substances in the endosperm and the embryo of...J. curcas and J. macrocarpa are useful for restoring degraded areas and their seeds contain oils for biodiesel production. The aim of the work was to determine the reserve substances in the endosperm and the embryo of J. curcas and J. macrocarpa which is important in understanding the germination process, the establishment of these species and its industrial employment. Seeds were imbibed in distilled water for 24 h, to facilitate removal of seed coat with the aim to separate the embryo and nutritive tissues. In both species, the endosperm contained aleurone grains consisting of a crystalloid and globoid, lipids of red color and the starch was not observed. Four major fatty acids were determined in J. curcas seed: oleic, palmitic, stearic, palmitoleic and oleic fatty acid represents about 70% oil content. Oleic acid was the most abundant in J. macrocarpa seeds, while, there was not palmitoleic acid. Seed with predominantly unsaturated fatty acids is ideal for biodiesel industry. The means of the sugar content were: 14.3 μg/mg in endosperm and 104.76 μg/mg in embryo of J. curcas and 6.48 μg/mg in endosperm and 59.20 μg/mg in embryo of J. macrocarpa. The means of the protein content were: 4.2 μg/mg in endosperm and 45.02 μg/mg in embryo of J. curcas and 3.26 μg/mg in endosperm and 31.08 μg/mg in embryo of J. macrocarpa. Sugar and protein contents of Jatropha seeds were significantly higher in embryo in both species (p 0.1), which suggests early mobilization towards the embryo during imbibition period.展开更多
Objective: To isolate new endophytic fungus from Phaleria macrocarpa(P. macrocarpa)that is able to produce E2.2 compound.Methods: Endophytic fungi were isolated from P. macrocarpa. Morphological and molecular identifi...Objective: To isolate new endophytic fungus from Phaleria macrocarpa(P. macrocarpa)that is able to produce E2.2 compound.Methods: Endophytic fungi were isolated from P. macrocarpa. Morphological and molecular identification was done to determine the species of the endophytic fungus.High performance liquid chromatography was used to determine the ability of this fungus to produce E2.2 compound and to quantify the total yield of E2.2 from fungal fermentation. Fermentation process was optimized by observing suitable medium, p H and length of fermentation process. Phloroglucinol and gallic acid addition were examined to determine the effect of each compound on E2.2 production.Results: One endophytic fungus was successfully isolated from P. macrocarpa plant.Morphological and molecular identification showed that it was a Colletotrichum gloeosporioides which belonged to Glomerellaceae family. This fungus showed highest production of E2.2 when incubated in potato dextrose broth with initial p H value of the medium at 5, and was incubated for 15 days. Phloroglucinol was found to better enhance E2.2 production.Conclusions: Colletotrichum gloeosporioides found in P. macrocarpa plant is promising as a potential alternative source of E2.2.展开更多
Objective: To investigate L-3, 4-dihydroxyphenylalanine(L-dopa, anti-Parkinson drug),anti-inflammatory activity, proximate nutritional composition and antioxidant potential of Mucuna macrocarpa(M. macrocarpa) beans.Me...Objective: To investigate L-3, 4-dihydroxyphenylalanine(L-dopa, anti-Parkinson drug),anti-inflammatory activity, proximate nutritional composition and antioxidant potential of Mucuna macrocarpa(M. macrocarpa) beans.Methods: L-dopa content was determined and quantified by high performance thin layer chromatography and reversed phase high-performance liquid chromatography(RPHPLC) methods. Anti-inflammatory activity was performed by in vitro protein denaturation inhibition and human red blood cell membrane stabilisation activity. Proximate composition and elemental analysis were also investigated. The antioxidant potential(2,2-diphenyl-1-picrylhydrazyl, N-N-dimethyl-phenylenediamine and ferric-reducing antioxidant power) of M. macrocarpa beans were evaluated by using different extraction solvents. The RP-HPLC analysis also quantified significant phenolics such as gallic acid, tannic acid, p-hydroxybenzoic acid and p-coumaric acid.Results: RP-HPLC quantification revealed that M. macrocarpa beans contain a high level of L-dopa [(115.41 ± 0.985) mg/g] which was the highest among the Mucuna species from Indian sub-continent. Water extract of seed powder showed strong antiinflammatory and antioxidant potential. Proximate composition of M. macrocarpa beans revealed numerous nutritional and anti-nutritional components. RP-HPLC analysis of major phenolics such as tannic acid(43.795 mg/g), gallic acid(0.864 mg/g), p-coumaric acid(0.364 mg/g) and p-hydroxybenzoic acid(0.036 mg/g) quantified successfully from M. macrocarpa beans respectively.Conclusions: This study suggests that M. macrocarpa is a potential source of L-dopa with promising anti-inflammatory, antioxidant and nutritional benefits.展开更多
Objective: To prove the molecular mechanisms of Mahkota Dewa(Phaleria macrocarpa) in suppressing proliferation of human retinoblastoma cells through suppression of cell cycle's gene-regulators expression.Methods: ...Objective: To prove the molecular mechanisms of Mahkota Dewa(Phaleria macrocarpa) in suppressing proliferation of human retinoblastoma cells through suppression of cell cycle's gene-regulators expression.Methods: In this study, the molecular mechanism of anti-tumor effect of fractioned extract of Phaleria macrocarpa(DLBS1425) in human retinoblastoma cells Y-79 was investigated by measuring the tumor cells viability, the assessment of population profiles of tumor cells in the cell cycle, and the mRNA concentration of p16, p21, p53, cyclin D,cyclin E, and E2 F.Results: DLBS1425 showed an inhibition effects towards proliferation of Y-79 cell line.Inhibition of proliferation was shown by suppression of cell cycle progression.DLBS1425 downregulated cyclin E, a G1 phase regulator gene of cell cycle, in dosedependent manner without affecting p53–p21 pathway.In the other word, DLBS1425 inhibits cell proliferation through suppression of cyclin E independently towards conventional proliferation pathway.Conclusions: Our results suggest that DLBS1425 is a potential anticancer agent which targets genes involved in cell proliferation in human retinoblastoma cells which make it pharmacologically ideal for the prevention and/or treatment of retinoblastoma cancer.展开更多
Objective: To identify and isolate the chemical compounds of Phaleria macrocarpa(P.macrocarpa) fruit ethanolic extract.Methods: Dried fruit of P.macrocarpa was extracted with 90% ethanol and partitioned between n-hexa...Objective: To identify and isolate the chemical compounds of Phaleria macrocarpa(P.macrocarpa) fruit ethanolic extract.Methods: Dried fruit of P.macrocarpa was extracted with 90% ethanol and partitioned between n-hexane/H_2O and ethyl acetate/H_2O.The organic layer was fractionated by various stationary phase and identified by using combined data of 1D [(proton nuclear magnetic resonance(NMR), carbon-13 NMR)], 2D-NMR(heteronuclear multiplequantum correlation and heteronuclear multiple-bond correlation), and mass spectrum.Results: Purification of n-hexane and ethyl acetate fractions from ethanolic extract of P.macrocarpa fruit resulted in isolation of nine compounds.Conclusions: A new compound was isolated and identified as glyceryl pentacosanoate.Also, two xanthones, which are 1,7-dihydroxy-3,6-dimethoxyxanthone and 1,6,7-trihydroxy-3-methoxyxanthone, are firstly reported to be isolated from P.macrocarpa.展开更多
This study compared the lipid yield,chemical composition,thermal and rheological behavior of Pachira macrocarpa seed oil(PSO)extracted with five different solvents(petroleum ether,n-hexane,ethyl acetate,isopropanol an...This study compared the lipid yield,chemical composition,thermal and rheological behavior of Pachira macrocarpa seed oil(PSO)extracted with five different solvents(petroleum ether,n-hexane,ethyl acetate,isopropanol and Folch solution(methanol/chloroform,v/v=1:2)).The results showed that the lipid yield of PSO extracted with ethyl acetate was the highest(29.25%).The main fatty acids of PSOs extracted by different solvents were palmitic acid(48.82-49.24%),linoleic acid(31.23-31.62%)and oleic acid(13.88-14.26%).Ethyl acetate was the most suitable solvent for extracting phytosterols(1756.43 mg/kg),n-hexane was the best solvent for enriching squalene(906.50 mg/kg),and isopropanol extraction was the most suitable for enriching tocopherols(436.90 mg/kg).Folch solution extraction can efficiently extract polyphenols and its oil had the best oxidative stability in all samples.Furthermore,PSOs showed high thermal stability and exhibited Newtonian flow behavior.Overall,this study provides guidance for the industrial production of PSO,and Folch solution extraction was the most suitable method for the development of PSO for the potential healthcare application.展开更多
Objective:To evaluate the cytotoxicity of Phaleria macrocarpa fruits extracts.Methods:The cytotoxicity test was carried out by in vitro MCF-7 cell line and in vivo brine shrimp lethality bioassay.Results:The prelimina...Objective:To evaluate the cytotoxicity of Phaleria macrocarpa fruits extracts.Methods:The cytotoxicity test was carried out by in vitro MCF-7 cell line and in vivo brine shrimp lethality bioassay.Results:The preliminary phytochemical test showed the presence of alkaloids,carbohydrate,glycosides,saponin,terpene,steroids,phenols and flavonoids.The MTT-assay results showed that the highest percentage of cell viability was 106.23%at concentration of 1.25μL and the lowest percentage was 13.04%at concentration of 10μL.Conclusions:The MTT-assay and brine shrimp lethality bioassay results showed that the extract was non-toxic and it would be consumable as a herbal remedy.展开更多
基金Supported by PT Dexa Medica(Grant No.125/MP/DLBS/2015)
文摘Objective: To verify that Proliverenol has a potential ability in protecting cells from ethanol-induced hepatotoxicity.Methods: Activity of Proliverenol against ethanol-induced apoptosis was evaluated at m RNA and protein levels in Hep G2 cell exposed to Proliverenol for 1 and 3 h.Results: Proliverenol conferred hepatoprotective activity through increasing cell survival up to 53%–69% via up-regulation of APEX1 DNA repair enzyme for 3.0–4.7 fold and down-regulating of nuclear factor-kB, tumor necrosis factora and caspase-8 expression,allowing them to prevent 4.5–6.9 fold of alanine aminotransferase(ALT) leakage in Hep G2 cells. Our finding revealed that Proliverenol repressed expression of ALT, which is significantly important as possible alternative mechanism for increased blood transaminase activities. In addition, the result also showed that caspase-8 pathway seemed to be involved in the molecular pathway rather than directly inducing mitochondrial damage.Conclusions: The data support our hypothesis that Proliverenol has a potential ability in protecting cells from ethanol-induced hepatotoxicity. We propose that Proliverenol provides hepatoprotective activity through up-regulating expression of APEX1 that repress DNA fragmentation, and down-regulating expression of nuclear factor-kB, tumor necrosis factora and caspase-8, which therefore repress ALT leakage and its expression.
文摘J. curcas and J. macrocarpa are useful for restoring degraded areas and their seeds contain oils for biodiesel production. The aim of the work was to determine the reserve substances in the endosperm and the embryo of J. curcas and J. macrocarpa which is important in understanding the germination process, the establishment of these species and its industrial employment. Seeds were imbibed in distilled water for 24 h, to facilitate removal of seed coat with the aim to separate the embryo and nutritive tissues. In both species, the endosperm contained aleurone grains consisting of a crystalloid and globoid, lipids of red color and the starch was not observed. Four major fatty acids were determined in J. curcas seed: oleic, palmitic, stearic, palmitoleic and oleic fatty acid represents about 70% oil content. Oleic acid was the most abundant in J. macrocarpa seeds, while, there was not palmitoleic acid. Seed with predominantly unsaturated fatty acids is ideal for biodiesel industry. The means of the sugar content were: 14.3 μg/mg in endosperm and 104.76 μg/mg in embryo of J. curcas and 6.48 μg/mg in endosperm and 59.20 μg/mg in embryo of J. macrocarpa. The means of the protein content were: 4.2 μg/mg in endosperm and 45.02 μg/mg in embryo of J. curcas and 3.26 μg/mg in endosperm and 31.08 μg/mg in embryo of J. macrocarpa. Sugar and protein contents of Jatropha seeds were significantly higher in embryo in both species (p 0.1), which suggests early mobilization towards the embryo during imbibition period.
文摘Objective: To isolate new endophytic fungus from Phaleria macrocarpa(P. macrocarpa)that is able to produce E2.2 compound.Methods: Endophytic fungi were isolated from P. macrocarpa. Morphological and molecular identification was done to determine the species of the endophytic fungus.High performance liquid chromatography was used to determine the ability of this fungus to produce E2.2 compound and to quantify the total yield of E2.2 from fungal fermentation. Fermentation process was optimized by observing suitable medium, p H and length of fermentation process. Phloroglucinol and gallic acid addition were examined to determine the effect of each compound on E2.2 production.Results: One endophytic fungus was successfully isolated from P. macrocarpa plant.Morphological and molecular identification showed that it was a Colletotrichum gloeosporioides which belonged to Glomerellaceae family. This fungus showed highest production of E2.2 when incubated in potato dextrose broth with initial p H value of the medium at 5, and was incubated for 15 days. Phloroglucinol was found to better enhance E2.2 production.Conclusions: Colletotrichum gloeosporioides found in P. macrocarpa plant is promising as a potential alternative source of E2.2.
基金supported by Department of Biotechnology,Government of India for Interdisciplinary Programme of Life Sciences for the Advanced Research and Education(IPLS–Reference No:BT/PR4572/INF/22/147/2012)
文摘Objective: To investigate L-3, 4-dihydroxyphenylalanine(L-dopa, anti-Parkinson drug),anti-inflammatory activity, proximate nutritional composition and antioxidant potential of Mucuna macrocarpa(M. macrocarpa) beans.Methods: L-dopa content was determined and quantified by high performance thin layer chromatography and reversed phase high-performance liquid chromatography(RPHPLC) methods. Anti-inflammatory activity was performed by in vitro protein denaturation inhibition and human red blood cell membrane stabilisation activity. Proximate composition and elemental analysis were also investigated. The antioxidant potential(2,2-diphenyl-1-picrylhydrazyl, N-N-dimethyl-phenylenediamine and ferric-reducing antioxidant power) of M. macrocarpa beans were evaluated by using different extraction solvents. The RP-HPLC analysis also quantified significant phenolics such as gallic acid, tannic acid, p-hydroxybenzoic acid and p-coumaric acid.Results: RP-HPLC quantification revealed that M. macrocarpa beans contain a high level of L-dopa [(115.41 ± 0.985) mg/g] which was the highest among the Mucuna species from Indian sub-continent. Water extract of seed powder showed strong antiinflammatory and antioxidant potential. Proximate composition of M. macrocarpa beans revealed numerous nutritional and anti-nutritional components. RP-HPLC analysis of major phenolics such as tannic acid(43.795 mg/g), gallic acid(0.864 mg/g), p-coumaric acid(0.364 mg/g) and p-hydroxybenzoic acid(0.036 mg/g) quantified successfully from M. macrocarpa beans respectively.Conclusions: This study suggests that M. macrocarpa is a potential source of L-dopa with promising anti-inflammatory, antioxidant and nutritional benefits.
基金Supported by The Dexa Laboratories of Biomolecular Sciences(DLBS),PT.Dexa Medica,Cikarang,West Java,Indonesia,Grant No.135/MP/DLBS/2016
文摘Objective: To prove the molecular mechanisms of Mahkota Dewa(Phaleria macrocarpa) in suppressing proliferation of human retinoblastoma cells through suppression of cell cycle's gene-regulators expression.Methods: In this study, the molecular mechanism of anti-tumor effect of fractioned extract of Phaleria macrocarpa(DLBS1425) in human retinoblastoma cells Y-79 was investigated by measuring the tumor cells viability, the assessment of population profiles of tumor cells in the cell cycle, and the mRNA concentration of p16, p21, p53, cyclin D,cyclin E, and E2 F.Results: DLBS1425 showed an inhibition effects towards proliferation of Y-79 cell line.Inhibition of proliferation was shown by suppression of cell cycle progression.DLBS1425 downregulated cyclin E, a G1 phase regulator gene of cell cycle, in dosedependent manner without affecting p53–p21 pathway.In the other word, DLBS1425 inhibits cell proliferation through suppression of cyclin E independently towards conventional proliferation pathway.Conclusions: Our results suggest that DLBS1425 is a potential anticancer agent which targets genes involved in cell proliferation in human retinoblastoma cells which make it pharmacologically ideal for the prevention and/or treatment of retinoblastoma cancer.
基金Supported by PT Dexa Medica with grant number 120/IMC/DLBS/2014
文摘Objective: To identify and isolate the chemical compounds of Phaleria macrocarpa(P.macrocarpa) fruit ethanolic extract.Methods: Dried fruit of P.macrocarpa was extracted with 90% ethanol and partitioned between n-hexane/H_2O and ethyl acetate/H_2O.The organic layer was fractionated by various stationary phase and identified by using combined data of 1D [(proton nuclear magnetic resonance(NMR), carbon-13 NMR)], 2D-NMR(heteronuclear multiplequantum correlation and heteronuclear multiple-bond correlation), and mass spectrum.Results: Purification of n-hexane and ethyl acetate fractions from ethanolic extract of P.macrocarpa fruit resulted in isolation of nine compounds.Conclusions: A new compound was isolated and identified as glyceryl pentacosanoate.Also, two xanthones, which are 1,7-dihydroxy-3,6-dimethoxyxanthone and 1,6,7-trihydroxy-3-methoxyxanthone, are firstly reported to be isolated from P.macrocarpa.
基金supported by National Natural Science Foundation of China(No.32160572)the Central Government Guides the Local Science and Technology Development Fund Project(20221ZDF04047 and 20221ZDF03029)+1 种基金the Research Program of State Key Laboratory of Food Science and Technology,Nanchang University(SKLF-ZZB-202114 and SKLF-ZZB-201922)the Science and Technology Plan Special Project of Ji′an City,China(20211-055347 and 20211-055486).
文摘This study compared the lipid yield,chemical composition,thermal and rheological behavior of Pachira macrocarpa seed oil(PSO)extracted with five different solvents(petroleum ether,n-hexane,ethyl acetate,isopropanol and Folch solution(methanol/chloroform,v/v=1:2)).The results showed that the lipid yield of PSO extracted with ethyl acetate was the highest(29.25%).The main fatty acids of PSOs extracted by different solvents were palmitic acid(48.82-49.24%),linoleic acid(31.23-31.62%)and oleic acid(13.88-14.26%).Ethyl acetate was the most suitable solvent for extracting phytosterols(1756.43 mg/kg),n-hexane was the best solvent for enriching squalene(906.50 mg/kg),and isopropanol extraction was the most suitable for enriching tocopherols(436.90 mg/kg).Folch solution extraction can efficiently extract polyphenols and its oil had the best oxidative stability in all samples.Furthermore,PSOs showed high thermal stability and exhibited Newtonian flow behavior.Overall,this study provides guidance for the industrial production of PSO,and Folch solution extraction was the most suitable method for the development of PSO for the potential healthcare application.
文摘Objective:To evaluate the cytotoxicity of Phaleria macrocarpa fruits extracts.Methods:The cytotoxicity test was carried out by in vitro MCF-7 cell line and in vivo brine shrimp lethality bioassay.Results:The preliminary phytochemical test showed the presence of alkaloids,carbohydrate,glycosides,saponin,terpene,steroids,phenols and flavonoids.The MTT-assay results showed that the highest percentage of cell viability was 106.23%at concentration of 1.25μL and the lowest percentage was 13.04%at concentration of 10μL.Conclusions:The MTT-assay and brine shrimp lethality bioassay results showed that the extract was non-toxic and it would be consumable as a herbal remedy.
