[Objectives] To study the effects of maca extract on the exercise endurance and ultrastructure of mitochondria in spinal neurons.[Methods]First,50 Wistar rats were randomly divided into 5 groups. The control group: no...[Objectives] To study the effects of maca extract on the exercise endurance and ultrastructure of mitochondria in spinal neurons.[Methods]First,50 Wistar rats were randomly divided into 5 groups. The control group: no swimming,administered intragastrically the same volume of distilled water; simple swimming group: free swimming,administered intragastrically the same volume of distilled water; maca extract groups: free swimming,and treated with 4. 0,5. 3,and 8. 0 g/kg dose of maca extracts. Swimming rats swam freely in the circulating water flow of swimming pool and administered for 15 d. On the 16 th d,after the swimming endurance test,the rats were killed painlessly. The ultrastructure of the mitochondria in the spinal neurons was observed with a projection electron microscope,muscle glycogen,malondialdehyde( MDA),superoxide dismutase( SOD),glutathione peroxidase( GSH-Px) and free calcium in muscle were measured by radioimmunoassay.[Results] Compared with the simple swimming group,the swimming time before sinking and total swimming time extended 19. 83%,60. 28%,77. 55% and 55. 34%,73. 91% and 94. 47% respectively,and the differences were statistically significant( P < 0. 01). The sinking times decreased by 34. 35%,51. 18% and 57. 96% respectively,the differences were statistically significant( P < 0. 01). MDA and free calcium decreased by 20. 10%,31. 49% and 38. 72%,respectively with statistically significant differences( P < 0. 01) and 6. 42%,17. 58%and 26. 35% respectively. The levels of SOD,GSH-Px and muscle glycogen increased by 5. 12%,22. 74%,52. 53%,44. 22%,77. 79%and 98. 45% respectively,with statistically significant differences( P < 0. 01) and 35. 08%,47. 83% and 81. 88%,respectively,with statistically significant differences( P < 0. 01). The volume density( Vd),surface density( Sd) and number density( Nd) of mitochondria of spinal neurons decreased by 7. 79%,18. 18%,31. 17%,16. 95%,27. 34%,43. 31% and 13. 51%,23. 19% and 43. 15% respectively.[Conclusions]Maca extract can protect ultrastructure of the mitochondria in the spinal neurons,antioxidant activity,increase muscle glycogen,and improving the exercise capacity.展开更多
[Objectives] The aim was to investigate the effects of maca extract on endurance and blood antioxidative enzyme activity of rats.[Methods]The Wistar rats treated with/without maca extract( 8. 0,16. 0,and 32. 0 g/kg) w...[Objectives] The aim was to investigate the effects of maca extract on endurance and blood antioxidative enzyme activity of rats.[Methods]The Wistar rats treated with/without maca extract( 8. 0,16. 0,and 32. 0 g/kg) were given with free access to circulating water.The administration was conducted for 15 consecutive d. On the 16 th d,the endurance and blood malondialdehyde( MDA),superoxide dismutase( SOD) and glutathione peroxidase( GSH-Px) levels of the rats were determined. [Results] Compared with those of the rats without maca extract treatment,the swimming time before sinking of the rats administered with 8. 0,16. 0,and 32. 0 g/kg of maca extract was increased by 34. 38%,84. 25% and 126. 07%( P < 0. 01),the total swimming time was increased by 37. 42%,47. 03% and 107. 24%( P <0. 01),the times of sinking was reduced by 17. 65%,33. 28% and 55. 50%( P < 0. 05,P < 0. 01),the blood MDA level was reduced by 34.63%,54. 13% and 63. 73%( P < 0. 01),the blood SOD activity was increased by 67. 78%,108. 25% and 154. 49%( P < 0. 01),and the blood GSH-Px level was increased by 48. 57%,110. 28% and 154. 86%( P < 0. 01). [Conclusions]Maca extract has the functions of resisting fatigue and enhancing athletic,and the mechanisms are related to the reduction of blood MDA level and the increase of blood SOD and GSH-Px activity.展开更多
Aim： To determine the effect of two different extracts of red maca in male rats. Methods： Prostatic hyperplasia was induced in male rats with testosterone enanthate （TE）. The study comprised six groups： one contr...Aim： To determine the effect of two different extracts of red maca in male rats. Methods： Prostatic hyperplasia was induced in male rats with testosterone enanthate （TE）. The study comprised six groups： one control group （group 1）, one group treated with TE （group 2）, two groups treated with TE and aqueous extract of red maca （groups 3 and 4）, one group treated with hydroalcoholic extract of red maca （group 5） and one group treated with finasteride （0.1 mg, group 6）. Differences in the aqueous extract dependent on the length of time of boiling, whether for 2 or 3 hours, for groups 3 and 4 was assessed. Extracts of red maca contained 0.1 mg of benzylglucosinolate. Thereafter, a doseresponse effect of different doses of benzylglucosinolates （0.02-0.08 mg） in red maca extracts was assessed. Results： Prostate weight was similar in rats treated with freeze-dried aqueous extract of red maca prepared after 2 and 3 hours of boiling. Freeze-dried aqueous extract of red maca, hydroalcoholic extract of red maca and finasteride reduced prostate weight in rats with prostatic hyperplasia. No difference was observed between the data obtained from aqueous extract or hydroalcoholic extract of red maca. A dose dependent reduction of prostate weight was observed with the increase of the dose of benzylglucosinolates in red maca extracts. Conclusion： The present study showed that hydroalcoholic or aqueous extract of red maca containing 0.1 mg of benzylglucosinolate can reduce prostate size in male rats in which prostatic hyperplasia had been induced by TE.展开更多
研究不同温度和超声密度下循环超声提取玛咖生物碱的动力学及热力学。分别采用双指数模型和范特霍夫方程用于提取动力学过程拟合和热力学参数计算。结果表明双指数模型能较好的拟合玛咖生物碱提取过程,生物碱得率和速率常数随着温度或...研究不同温度和超声密度下循环超声提取玛咖生物碱的动力学及热力学。分别采用双指数模型和范特霍夫方程用于提取动力学过程拟合和热力学参数计算。结果表明双指数模型能较好的拟合玛咖生物碱提取过程,生物碱得率和速率常数随着温度或超声密度的增加而增加,洗涤和扩散阶段的活化能分别为16.41 k J/mol和17.52 k J/mol。循化超声提取过程是吸热、不可逆和自发的。与传统浸提相比,循环超声提取在20℃和50℃时,生物碱得率均显著高于传统浸提(p<0.01)。因此循环超声提取是一种高效的提取技术,可用于玛咖生物碱提取。展开更多
基金Supported by Science and Technology Planning Project of Lanzhou City(2015-3-80)Science and Technology Innovation Team Project for Universities in Gansu Province(2016C-09)+3 种基金Talent Innovation and Entrepreneurship Project of Lanzhou City(2016-RC-85)Gansu Natural Science Foundation Project(17JR5RA158)Investigation Theory and Practice Research Center Project of Northwest Minority AreasGansu Province Evidence Science Characteristic Discipline Project
文摘[Objectives] To study the effects of maca extract on the exercise endurance and ultrastructure of mitochondria in spinal neurons.[Methods]First,50 Wistar rats were randomly divided into 5 groups. The control group: no swimming,administered intragastrically the same volume of distilled water; simple swimming group: free swimming,administered intragastrically the same volume of distilled water; maca extract groups: free swimming,and treated with 4. 0,5. 3,and 8. 0 g/kg dose of maca extracts. Swimming rats swam freely in the circulating water flow of swimming pool and administered for 15 d. On the 16 th d,after the swimming endurance test,the rats were killed painlessly. The ultrastructure of the mitochondria in the spinal neurons was observed with a projection electron microscope,muscle glycogen,malondialdehyde( MDA),superoxide dismutase( SOD),glutathione peroxidase( GSH-Px) and free calcium in muscle were measured by radioimmunoassay.[Results] Compared with the simple swimming group,the swimming time before sinking and total swimming time extended 19. 83%,60. 28%,77. 55% and 55. 34%,73. 91% and 94. 47% respectively,and the differences were statistically significant( P < 0. 01). The sinking times decreased by 34. 35%,51. 18% and 57. 96% respectively,the differences were statistically significant( P < 0. 01). MDA and free calcium decreased by 20. 10%,31. 49% and 38. 72%,respectively with statistically significant differences( P < 0. 01) and 6. 42%,17. 58%and 26. 35% respectively. The levels of SOD,GSH-Px and muscle glycogen increased by 5. 12%,22. 74%,52. 53%,44. 22%,77. 79%and 98. 45% respectively,with statistically significant differences( P < 0. 01) and 35. 08%,47. 83% and 81. 88%,respectively,with statistically significant differences( P < 0. 01). The volume density( Vd),surface density( Sd) and number density( Nd) of mitochondria of spinal neurons decreased by 7. 79%,18. 18%,31. 17%,16. 95%,27. 34%,43. 31% and 13. 51%,23. 19% and 43. 15% respectively.[Conclusions]Maca extract can protect ultrastructure of the mitochondria in the spinal neurons,antioxidant activity,increase muscle glycogen,and improving the exercise capacity.
基金Supported by Program for Innovative Research Team in Science and Technology in University of Gansu Province(2016C-09)Natural Science Foundation of Gansu Province(17JR5RA158)+1 种基金Innovative and Entrepreneurial Talent Program of Lanzhou City(2016-RC-85)Project of National Research Center for Investigation Theory and Practice in Northwest Ethnic Regions
文摘[Objectives] The aim was to investigate the effects of maca extract on endurance and blood antioxidative enzyme activity of rats.[Methods]The Wistar rats treated with/without maca extract( 8. 0,16. 0,and 32. 0 g/kg) were given with free access to circulating water.The administration was conducted for 15 consecutive d. On the 16 th d,the endurance and blood malondialdehyde( MDA),superoxide dismutase( SOD) and glutathione peroxidase( GSH-Px) levels of the rats were determined. [Results] Compared with those of the rats without maca extract treatment,the swimming time before sinking of the rats administered with 8. 0,16. 0,and 32. 0 g/kg of maca extract was increased by 34. 38%,84. 25% and 126. 07%( P < 0. 01),the total swimming time was increased by 37. 42%,47. 03% and 107. 24%( P <0. 01),the times of sinking was reduced by 17. 65%,33. 28% and 55. 50%( P < 0. 05,P < 0. 01),the blood MDA level was reduced by 34.63%,54. 13% and 63. 73%( P < 0. 01),the blood SOD activity was increased by 67. 78%,108. 25% and 154. 49%( P < 0. 01),and the blood GSH-Px level was increased by 48. 57%,110. 28% and 154. 86%( P < 0. 01). [Conclusions]Maca extract has the functions of resisting fatigue and enhancing athletic,and the mechanisms are related to the reduction of blood MDA level and the increase of blood SOD and GSH-Px activity.
文摘Aim： To determine the effect of two different extracts of red maca in male rats. Methods： Prostatic hyperplasia was induced in male rats with testosterone enanthate （TE）. The study comprised six groups： one control group （group 1）, one group treated with TE （group 2）, two groups treated with TE and aqueous extract of red maca （groups 3 and 4）, one group treated with hydroalcoholic extract of red maca （group 5） and one group treated with finasteride （0.1 mg, group 6）. Differences in the aqueous extract dependent on the length of time of boiling, whether for 2 or 3 hours, for groups 3 and 4 was assessed. Extracts of red maca contained 0.1 mg of benzylglucosinolate. Thereafter, a doseresponse effect of different doses of benzylglucosinolates （0.02-0.08 mg） in red maca extracts was assessed. Results： Prostate weight was similar in rats treated with freeze-dried aqueous extract of red maca prepared after 2 and 3 hours of boiling. Freeze-dried aqueous extract of red maca, hydroalcoholic extract of red maca and finasteride reduced prostate weight in rats with prostatic hyperplasia. No difference was observed between the data obtained from aqueous extract or hydroalcoholic extract of red maca. A dose dependent reduction of prostate weight was observed with the increase of the dose of benzylglucosinolates in red maca extracts. Conclusion： The present study showed that hydroalcoholic or aqueous extract of red maca containing 0.1 mg of benzylglucosinolate can reduce prostate size in male rats in which prostatic hyperplasia had been induced by TE.
文摘研究不同温度和超声密度下循环超声提取玛咖生物碱的动力学及热力学。分别采用双指数模型和范特霍夫方程用于提取动力学过程拟合和热力学参数计算。结果表明双指数模型能较好的拟合玛咖生物碱提取过程,生物碱得率和速率常数随着温度或超声密度的增加而增加,洗涤和扩散阶段的活化能分别为16.41 k J/mol和17.52 k J/mol。循化超声提取过程是吸热、不可逆和自发的。与传统浸提相比,循环超声提取在20℃和50℃时,生物碱得率均显著高于传统浸提(p<0.01)。因此循环超声提取是一种高效的提取技术,可用于玛咖生物碱提取。
