Background： Cellular non-coding RNAs are extensively modified post-transcriptionally, with more than 100 chemically distinct nucleotides identified to date. In the past five years, new sequencing based methods have r...Background： Cellular non-coding RNAs are extensively modified post-transcriptionally, with more than 100 chemically distinct nucleotides identified to date. In the past five years, new sequencing based methods have revealed widespread decoration of eukaryotic messenger RNA with diverse RNA modifications whose functions in mRNA metabolism are only beginning to be known. Results： Since most of the identified mRNA modifying enzymes are present in the nucleus, these modifications have the potential to function in nuclear pre-mRNA processing including alternative splicing. Here we review recent progress towards illuminating the role of pre-mRNA modifications in splicing and highlight key areas for future investigation in this rapidly growing field. Conclusions： Future studies to identify which modifications are added to nascent pre-mRNA and to interrogate the direct effects of individual modifications are likely to reveal new mechanisms by which nuclear pre-mRNA processing is regulated.展开更多
N4-acetylcytidine(ac^(4)C)is a conserved acetylation modification on messenger RNA(mRNA)that was recently identified in plants.It is deposited by the N-ACETYLTRANSFERASE FOR CYTIDINE IN RNA(ACYR)protein,which is homol...N4-acetylcytidine(ac^(4)C)is a conserved acetylation modification on messenger RNA(mRNA)that was recently identified in plants.It is deposited by the N-ACETYLTRANSFERASE FOR CYTIDINE IN RNA(ACYR)protein,which is homologous to the mammalian N-ACETYLTRANSFERASE NAT10(Li et al.,2023;Wang et al.,2023).展开更多
文摘Background： Cellular non-coding RNAs are extensively modified post-transcriptionally, with more than 100 chemically distinct nucleotides identified to date. In the past five years, new sequencing based methods have revealed widespread decoration of eukaryotic messenger RNA with diverse RNA modifications whose functions in mRNA metabolism are only beginning to be known. Results： Since most of the identified mRNA modifying enzymes are present in the nucleus, these modifications have the potential to function in nuclear pre-mRNA processing including alternative splicing. Here we review recent progress towards illuminating the role of pre-mRNA modifications in splicing and highlight key areas for future investigation in this rapidly growing field. Conclusions： Future studies to identify which modifications are added to nascent pre-mRNA and to interrogate the direct effects of individual modifications are likely to reveal new mechanisms by which nuclear pre-mRNA processing is regulated.
基金supported by the National Natural Science Foundation of China(grant nos.32441032 and 32371365)the Fundamental Research Funds for the Central Universities(grant no.KJYQ2025014)the Natural Science Foundation of Jiangsu Province,China(grant no.BK20230101).
文摘N4-acetylcytidine(ac^(4)C)is a conserved acetylation modification on messenger RNA(mRNA)that was recently identified in plants.It is deposited by the N-ACETYLTRANSFERASE FOR CYTIDINE IN RNA(ACYR)protein,which is homologous to the mammalian N-ACETYLTRANSFERASE NAT10(Li et al.,2023;Wang et al.,2023).
