Aim: To evaluate the effects of 1,3-dinitrobenznene (mDNB) on sperm motility of hamster and to correlate the re-sults with the fertility. Methods: Adult male hamsters were gavaged with one of the 3 dose regimes of mDN...Aim: To evaluate the effects of 1,3-dinitrobenznene (mDNB) on sperm motility of hamster and to correlate the re-sults with the fertility. Methods: Adult male hamsters were gavaged with one of the 3 dose regimes of mDNB(1.5 mg daily for 4 weeks ,1.5 mg one day a week for 4 weeks and 1.0 mg 3 days a week for 4 weeks). Computerassisted semen analysis (CASA) was used to analyse the sperm motility parameters, curvilinear velocity (VCL) andstraight line velocity (VSL) of sperm in distal corpus epididymides and distal cauda epididymides. In vitro fertilisationwas carried out only for 1.5 mg mDNB daily group to determine the sperm fertilising capacity. Results: There wasa significant reduction in sperm velocity parameters at weeks 3 and 4 after treatment, which was correlated with a de-cline in sperm fertility. Conclusion; Sperm velocity parameters may be used to determine the effect of a toxic insulton the sperm function.展开更多
文摘Aim: To evaluate the effects of 1,3-dinitrobenznene (mDNB) on sperm motility of hamster and to correlate the re-sults with the fertility. Methods: Adult male hamsters were gavaged with one of the 3 dose regimes of mDNB(1.5 mg daily for 4 weeks ,1.5 mg one day a week for 4 weeks and 1.0 mg 3 days a week for 4 weeks). Computerassisted semen analysis (CASA) was used to analyse the sperm motility parameters, curvilinear velocity (VCL) andstraight line velocity (VSL) of sperm in distal corpus epididymides and distal cauda epididymides. In vitro fertilisationwas carried out only for 1.5 mg mDNB daily group to determine the sperm fertilising capacity. Results: There wasa significant reduction in sperm velocity parameters at weeks 3 and 4 after treatment, which was correlated with a de-cline in sperm fertility. Conclusion; Sperm velocity parameters may be used to determine the effect of a toxic insulton the sperm function.
