Objective To isolate and characterize indigenous algicidal bacteria and their algae-lysing compounds active against Microcystis aeruginosa, strains TH1, TH2, and FACHB 905. Methods The bacteria were identified using t...Objective To isolate and characterize indigenous algicidal bacteria and their algae-lysing compounds active against Microcystis aeruginosa, strains TH1, TH2, and FACHB 905. Methods The bacteria were identified using the Biolog automated microbial identification system and 16S rDNA sequence analysis. The algae-lysing compounds were isolated and purified by silica gel column chromatography and reverse-phase high performance liquid chromatography. Their structures were confirmed by Nuclear Magnetic Resonance （NMR） and Fourier Transform infrared （FT-IR） spectroscopy. Algae-lysing activity was observed using microscopy. Results The algae-lysing bacterium LTH-2 isolated from Lake Taihu was identified as Serratia marcescens. Strain LTH-2 secreted a red pigment identified as prodigiosin （C20H25N30）, which showed strong lytic activity with algal strains M. aeruginoso TH1, TH2, and FACHB 905 in a concentration-dependent manner. The 50% inhibitory concentration （ICs0） of prodigiosin with the algal strains was 4.8 （±0.4）×10^-2 μg/mL, 8.9 （±1.1）×10^-2μg/mL, and 1.7 （±0.1）×10^-1 μg/mL in 24 h, respectively. Conclusion The bacterium LTH-2 and its pigment related to damage of cell membranes. The bacterium for regulating blooms of harmful M. aeruginosa. had strong Microcystis-lysing activity probably LTH-2 and its red pigment are potentially useful展开更多
目的:研究红细胞(red blood cell,RBC)裂解液对CD34^+细胞相对计数的影响。方法:收集101份外周血干细胞采集物,CD34-PE及CD45-FITC双色免疫荧光染色后,分别用FACS裂解液(FACSTM lysing solution,FACS)、Optilyse C裂解液(optilyse C lys...目的:研究红细胞(red blood cell,RBC)裂解液对CD34^+细胞相对计数的影响。方法:收集101份外周血干细胞采集物,CD34-PE及CD45-FITC双色免疫荧光染色后,分别用FACS裂解液(FACSTM lysing solution,FACS)、Optilyse C裂解液(optilyse C lysing solution,Optilyse C)及自配裂解液溶解红细胞,采用洗涤程序和运用ISHAGE设门策略,检测CD34^+细胞、淋巴、粒、单核细胞相对数,CD45^+细胞占全部收集信号百分比及各类细胞前向散射光(forward scatter,FSC)及侧向散射光(side scatter,SSC)信号。结果:比较3种裂解液处理后样本的CD34^+细胞百分比,FACS裂解液处理的样本CD34^+细胞百分比最低,自配裂解液最高(FACS vs. Optilyse C vs.自配=0.49±0.43 vs. 0.67±0.50 vs. 0.73±0.66,P=0.000)。FACS裂解后样本CD45+细胞百分比远高于Optilyse C及自配裂解液(t=142.500,P=0.000)。3种裂解液对淋巴细胞百分比结果(F=35.340,P=0.000)具有统计学差异;而粒细胞百分比(F=0.610,P=0.540)、单核细胞百分比(F=1.590,P=0.210)差异无统计学意义。FACS裂解液处理后,CD34^+细胞、淋巴细胞的FSC和SSC信号强度均弱于Optilyse C裂解液及自配的裂解液;而粒、单核细胞的SSC信号,FACS裂解液处理后最强,Optilyse C裂解液处理后最弱。另外,动员后的白细胞数多少与CD34^+细胞百分比不存在相关性(r=0.108,P=0.312)。结论:红细胞裂解液导致CD34^+细胞相对计数差异的原因可能与CD45^+细胞比例有关,即获取的有效细胞数;裂解液对细胞形态的影响与造成淋巴细胞比例有显著性差异有关。鉴于不同红细胞裂解液对CD34等抗原阳性细胞的相对计数确实有不良影响,因此在应用流式细胞术检测或计数时应慎重选择。展开更多
基金supported by the National Science and Technology Major Project (2012ZX07101-005)the National Natural Science Foundation of China (30972440)Jiangsu Province Postgraduate Innovation Project (CX10B-087Z)
文摘Objective To isolate and characterize indigenous algicidal bacteria and their algae-lysing compounds active against Microcystis aeruginosa, strains TH1, TH2, and FACHB 905. Methods The bacteria were identified using the Biolog automated microbial identification system and 16S rDNA sequence analysis. The algae-lysing compounds were isolated and purified by silica gel column chromatography and reverse-phase high performance liquid chromatography. Their structures were confirmed by Nuclear Magnetic Resonance （NMR） and Fourier Transform infrared （FT-IR） spectroscopy. Algae-lysing activity was observed using microscopy. Results The algae-lysing bacterium LTH-2 isolated from Lake Taihu was identified as Serratia marcescens. Strain LTH-2 secreted a red pigment identified as prodigiosin （C20H25N30）, which showed strong lytic activity with algal strains M. aeruginoso TH1, TH2, and FACHB 905 in a concentration-dependent manner. The 50% inhibitory concentration （ICs0） of prodigiosin with the algal strains was 4.8 （±0.4）×10^-2 μg/mL, 8.9 （±1.1）×10^-2μg/mL, and 1.7 （±0.1）×10^-1 μg/mL in 24 h, respectively. Conclusion The bacterium LTH-2 and its pigment related to damage of cell membranes. The bacterium for regulating blooms of harmful M. aeruginosa. had strong Microcystis-lysing activity probably LTH-2 and its red pigment are potentially useful
文摘目的:研究红细胞(red blood cell,RBC)裂解液对CD34^+细胞相对计数的影响。方法:收集101份外周血干细胞采集物,CD34-PE及CD45-FITC双色免疫荧光染色后,分别用FACS裂解液(FACSTM lysing solution,FACS)、Optilyse C裂解液(optilyse C lysing solution,Optilyse C)及自配裂解液溶解红细胞,采用洗涤程序和运用ISHAGE设门策略,检测CD34^+细胞、淋巴、粒、单核细胞相对数,CD45^+细胞占全部收集信号百分比及各类细胞前向散射光(forward scatter,FSC)及侧向散射光(side scatter,SSC)信号。结果:比较3种裂解液处理后样本的CD34^+细胞百分比,FACS裂解液处理的样本CD34^+细胞百分比最低,自配裂解液最高(FACS vs. Optilyse C vs.自配=0.49±0.43 vs. 0.67±0.50 vs. 0.73±0.66,P=0.000)。FACS裂解后样本CD45+细胞百分比远高于Optilyse C及自配裂解液(t=142.500,P=0.000)。3种裂解液对淋巴细胞百分比结果(F=35.340,P=0.000)具有统计学差异;而粒细胞百分比(F=0.610,P=0.540)、单核细胞百分比(F=1.590,P=0.210)差异无统计学意义。FACS裂解液处理后,CD34^+细胞、淋巴细胞的FSC和SSC信号强度均弱于Optilyse C裂解液及自配的裂解液;而粒、单核细胞的SSC信号,FACS裂解液处理后最强,Optilyse C裂解液处理后最弱。另外,动员后的白细胞数多少与CD34^+细胞百分比不存在相关性(r=0.108,P=0.312)。结论:红细胞裂解液导致CD34^+细胞相对计数差异的原因可能与CD45^+细胞比例有关,即获取的有效细胞数;裂解液对细胞形态的影响与造成淋巴细胞比例有显著性差异有关。鉴于不同红细胞裂解液对CD34等抗原阳性细胞的相对计数确实有不良影响,因此在应用流式细胞术检测或计数时应慎重选择。
