The main objective of this study was to investigate the presence of natural endophytic Beauveria bassiana within date palm tissues using molecular technique and measure their field efficacies in controlling Dubas bug,...The main objective of this study was to investigate the presence of natural endophytic Beauveria bassiana within date palm tissues using molecular technique and measure their field efficacies in controlling Dubas bug, Ornmatissus lybicus （Deberg）. Two entomopathogenic B. bassiana isolates （MARD 108 and 100） were isolated from date palm, Phoenix dactylifera L. leaves; in addition, one isolate （MARD 92） originally from soil was identified to have endophytic property. Concentration of 1 × 10^9 conidia/mL of each of three endophytic isolates was used in field experiments targeting Dubas bug nymphs via injection tree trunks. The results indicated that the high mortality rates reached 92%, 96% and 100% with infliction of the three endophytic isolates after 15 d from the treatment. The successful establishment of the fungal isolates in the date palm tissue was determined using B. bassiana species-specific primer for the first time via using conventional polymerase chain reaction （PCR） amplification technique before and after injection, and the positive gel band representation was the identification signs. The novel results depicted for the first time the presence of natural endophytic B. bassiana isolates within date palm tissues and their field efficacies in controlling Dubas bug, O. lybicus （Deberg） infestation.展开更多
文摘The main objective of this study was to investigate the presence of natural endophytic Beauveria bassiana within date palm tissues using molecular technique and measure their field efficacies in controlling Dubas bug, Ornmatissus lybicus （Deberg）. Two entomopathogenic B. bassiana isolates （MARD 108 and 100） were isolated from date palm, Phoenix dactylifera L. leaves; in addition, one isolate （MARD 92） originally from soil was identified to have endophytic property. Concentration of 1 × 10^9 conidia/mL of each of three endophytic isolates was used in field experiments targeting Dubas bug nymphs via injection tree trunks. The results indicated that the high mortality rates reached 92%, 96% and 100% with infliction of the three endophytic isolates after 15 d from the treatment. The successful establishment of the fungal isolates in the date palm tissue was determined using B. bassiana species-specific primer for the first time via using conventional polymerase chain reaction （PCR） amplification technique before and after injection, and the positive gel band representation was the identification signs. The novel results depicted for the first time the presence of natural endophytic B. bassiana isolates within date palm tissues and their field efficacies in controlling Dubas bug, O. lybicus （Deberg） infestation.
