A variety of luminol derivatives with N-and O-substitution have been synthesized with broad functional group tolerance. O-esterification has been demonstrated for the first time as a promising way to prepare enhanced ...A variety of luminol derivatives with N-and O-substitution have been synthesized with broad functional group tolerance. O-esterification has been demonstrated for the first time as a promising way to prepare enhanced CL reagents for sensing hemin, bloodstain and horseradish peroxidase(HRP). The most effective analogue with a deuterated acetyl group exhibited greater potential than luminol for bloodstain imaging and HRP imaging in western blotting(WB). In addition, O-etherification can greatly suppress CL signal that has been applied to design a CL probe for β-glucosidase(β-Glu). This study offers important and useful information regarding the luminol modification and shows great potential to use O-substituted analogues for enhanced CL analysis.展开更多
EDTA was used as an enhancer for Fe 2+ catalyzed light emission from luminol oxidation by dissolved oxygen. As a result, the limit of detection for ferrous ion with flow injection analysis was improved by a fact...EDTA was used as an enhancer for Fe 2+ catalyzed light emission from luminol oxidation by dissolved oxygen. As a result, the limit of detection for ferrous ion with flow injection analysis was improved by a factor of 160 by addition of EDTA to the luminol solution. Fe 2+ and Fe 3+ were determined simultaneously with a novel copper-coated zinc reductor minicolumn installed in one of the shunt after sample splitting in the manifold. The reductor minicolumn can be used for 3000 determinations at least. The dynamic range of determination was 1×10 -9 ～1×10 -5 mol·L -1 , with the limit of detection of 2.7×10 10 and 3.5×10 10 mol·L 1 ,for Fe 2+ and Fe 3+ , respectively. The preci sion for determination of 2×10 7 mol·L 1 of Fe 2+ and Fe 3+ was 2.3% and 4.0% (n=8), respectively, at a sampling rate of 60 h -1 . Cr 3+ and Co 2+ interfere. Fe 2+ and Fe 3+ in mixture were determined with satisfactory results. Samples of Fe 2+ and Fe 3+ were determined simultaneously and the results in good agreement with the standard spectrophotometric method. Indications were shown that EDTA functions as an enhancer, Fe 2+ as a catalyst, and oxygen is the oxidant of the chemiluminescent reaction, and the mechanism of the reaction was discussed.展开更多
基金projects of the Department of Education of Guangdong Province(Nos.2021ZDZX2030 and 2020ZDZX2017)Guangdong Basic and Applied Basic Research Foundation(No.2021A1515110115)Guangzhou Basic and Applied Basic Research Foundation(No.202102020548)for funding support。
文摘A variety of luminol derivatives with N-and O-substitution have been synthesized with broad functional group tolerance. O-esterification has been demonstrated for the first time as a promising way to prepare enhanced CL reagents for sensing hemin, bloodstain and horseradish peroxidase(HRP). The most effective analogue with a deuterated acetyl group exhibited greater potential than luminol for bloodstain imaging and HRP imaging in western blotting(WB). In addition, O-etherification can greatly suppress CL signal that has been applied to design a CL probe for β-glucosidase(β-Glu). This study offers important and useful information regarding the luminol modification and shows great potential to use O-substituted analogues for enhanced CL analysis.
文摘EDTA was used as an enhancer for Fe 2+ catalyzed light emission from luminol oxidation by dissolved oxygen. As a result, the limit of detection for ferrous ion with flow injection analysis was improved by a factor of 160 by addition of EDTA to the luminol solution. Fe 2+ and Fe 3+ were determined simultaneously with a novel copper-coated zinc reductor minicolumn installed in one of the shunt after sample splitting in the manifold. The reductor minicolumn can be used for 3000 determinations at least. The dynamic range of determination was 1×10 -9 ～1×10 -5 mol·L -1 , with the limit of detection of 2.7×10 10 and 3.5×10 10 mol·L 1 ,for Fe 2+ and Fe 3+ , respectively. The preci sion for determination of 2×10 7 mol·L 1 of Fe 2+ and Fe 3+ was 2.3% and 4.0% (n=8), respectively, at a sampling rate of 60 h -1 . Cr 3+ and Co 2+ interfere. Fe 2+ and Fe 3+ in mixture were determined with satisfactory results. Samples of Fe 2+ and Fe 3+ were determined simultaneously and the results in good agreement with the standard spectrophotometric method. Indications were shown that EDTA functions as an enhancer, Fe 2+ as a catalyst, and oxygen is the oxidant of the chemiluminescent reaction, and the mechanism of the reaction was discussed.
