Eight new diterpenoids,Isodons A-H(1-8),comprising seco-abietane and abietane-type structures,together with 13 known analogues(9-21),were isolated from Isodon lophanthoides(Buch.-Ham.ex D.Don)Hara.The compounds(+)-3/(...Eight new diterpenoids,Isodons A-H(1-8),comprising seco-abietane and abietane-type structures,together with 13 known analogues(9-21),were isolated from Isodon lophanthoides(Buch.-Ham.ex D.Don)Hara.The compounds(+)-3/(-)-3,(+)-4/(-)-4,and(+)-5/(-)-5 were identified as three enantiomeric pairs.The planar structures and absolute configurations of 1-8 were determined through high-resolution electrospray ionization mass spectrometry(HR-ESI-MS),1D&2D nuclear magnetic resonance(NMR)spectroscopy,electronic circular dichroism(ECD)calculations,and X-ray diffraction crystallography.A cholesterol 7α-hydroxylase(Cyp7a1)luciferase reporter assay revealed significant anti-cholestatic activities for compounds 1,(+)-4,6,7,12-14,and 16.Additionally,compound 6 demonstrated anti-cholestatic effects through the farnesoid X receptor(FXR)-associated signaling pathways in vitro and in vivo.These findings suggest potential applications for I.Lophanthoides in pharmaceutical development.展开更多
A new lignan, together with a known one, was isolated from Isodon lophanthoides var. gerardianus [Bentham] H. Hara. The structure of the new lignan was elucidated as 1_acetoxyl_2e_piperonyl_6e_[6_methoxyl_pi...A new lignan, together with a known one, was isolated from Isodon lophanthoides var. gerardianus [Bentham] H. Hara. The structure of the new lignan was elucidated as 1_acetoxyl_2e_piperonyl_6e_[6_methoxyl_piperonyl]_3,7_dioxabicyclo_[3,3,0]_octane mainly by 1D and 2D NMR techniques.展开更多
[ Objective] This study aimed to conduct the karyotype analysis of Rabdosia lophanthoides var. gerardians and R. lophanthoides Hara var. gracilifloruschromosomes. [ Method] Vigorously growing shoot tips of R. lophanth...[ Objective] This study aimed to conduct the karyotype analysis of Rabdosia lophanthoides var. gerardians and R. lophanthoides Hara var. gracilifloruschromosomes. [ Method] Vigorously growing shoot tips of R. lophanthoides var. gerardianus and R. lophanthoides Hara var. graciliflorus were stained for section preparation to observe and analyze the chromosomes. [ Result] Karyotype formula of R. lophanthoides var. gerardianus is K （2n） =2x =24 = 18m ＋4Sm, sugges- ting that this species belongs to category 2A of Stebbins; karyotype formula of R. lophanthoides Hara var. graciliflorus is K （2n） =2x =24 = 16m ＋8Sm, sugges- ting that this species belongs to category 1B of Stebbins. [Conclusion] R. lophanthoides var. gerardianus is more primitive compared with R. lophanthoides Hara var. graciliflorus.展开更多
A new phenylethanoid glycoside, 3-hydroxy-4-methoxy-β-phenylethoxy-O-α-L-rhamnopyranosyl-（1 → 3）-2-O-acetyl-O-β-D- glucopyranoside, named lophanthoside A, was isolated from Rabdosia lophanthoides （Buch.-Ham.ex ...A new phenylethanoid glycoside, 3-hydroxy-4-methoxy-β-phenylethoxy-O-α-L-rhamnopyranosyl-（1 → 3）-2-O-acetyl-O-β-D- glucopyranoside, named lophanthoside A, was isolated from Rabdosia lophanthoides （Buch.-Ham.ex D.Don） Hara. Its structure was determined by spectroscopic evidences.展开更多
The structures of two new abietane quinones, named micranthins A and B, were determined to be 7a-methoxy-14, 16-epoxy-8, 13-abietadiene-11, 12-dione (1) and 16-acetoxy-6, 7-dehydroroyleanone (2) respectively, which we...The structures of two new abietane quinones, named micranthins A and B, were determined to be 7a-methoxy-14, 16-epoxy-8, 13-abietadiene-11, 12-dione (1) and 16-acetoxy-6, 7-dehydroroyleanone (2) respectively, which were isolated from Isodon lophanthoides var. micranthus.展开更多
Gas chromatography/mass spectrometry (GC MS) of the essential oil from the aerial parts of Rabdosia lophanthoides resulted in the identification of 108 compounds representing 78.120% of the oil. Hydro distillati...Gas chromatography/mass spectrometry (GC MS) of the essential oil from the aerial parts of Rabdosia lophanthoides resulted in the identification of 108 compounds representing 78.120% of the oil. Hydro distillation of Rabdosia lophanthoides yielded a pale yellow oil. The compounds identified and their relative proportions are listed in Table 1 according to their order of elution on an HP 5MS capillary column. .展开更多
基金supported by the National Key R&D Program of China(No.2023YFD1601400)Jiangsu Outstanding Youth Fund Project(No.BK20231535)+2 种基金the National Natural Science Foundation of China(Nos.82074068 and 82430118)the Basic Research Project for the Development of Modern Industrial College of Traditional Chinese Medicine and Health at Lishui University,Specialized Research Funds from the State Key Laboratory of Natural Medicines,China Pharmaceutical University(SKLNMZZ2024JS25)the 111 Project(No.B18056)。
文摘Eight new diterpenoids,Isodons A-H(1-8),comprising seco-abietane and abietane-type structures,together with 13 known analogues(9-21),were isolated from Isodon lophanthoides(Buch.-Ham.ex D.Don)Hara.The compounds(+)-3/(-)-3,(+)-4/(-)-4,and(+)-5/(-)-5 were identified as three enantiomeric pairs.The planar structures and absolute configurations of 1-8 were determined through high-resolution electrospray ionization mass spectrometry(HR-ESI-MS),1D&2D nuclear magnetic resonance(NMR)spectroscopy,electronic circular dichroism(ECD)calculations,and X-ray diffraction crystallography.A cholesterol 7α-hydroxylase(Cyp7a1)luciferase reporter assay revealed significant anti-cholestatic activities for compounds 1,(+)-4,6,7,12-14,and 16.Additionally,compound 6 demonstrated anti-cholestatic effects through the farnesoid X receptor(FXR)-associated signaling pathways in vitro and in vivo.These findings suggest potential applications for I.Lophanthoides in pharmaceutical development.
文摘A new lignan, together with a known one, was isolated from Isodon lophanthoides var. gerardianus [Bentham] H. Hara. The structure of the new lignan was elucidated as 1_acetoxyl_2e_piperonyl_6e_[6_methoxyl_piperonyl]_3,7_dioxabicyclo_[3,3,0]_octane mainly by 1D and 2D NMR techniques.
文摘[ Objective] This study aimed to conduct the karyotype analysis of Rabdosia lophanthoides var. gerardians and R. lophanthoides Hara var. gracilifloruschromosomes. [ Method] Vigorously growing shoot tips of R. lophanthoides var. gerardianus and R. lophanthoides Hara var. graciliflorus were stained for section preparation to observe and analyze the chromosomes. [ Result] Karyotype formula of R. lophanthoides var. gerardianus is K （2n） =2x =24 = 18m ＋4Sm, sugges- ting that this species belongs to category 2A of Stebbins; karyotype formula of R. lophanthoides Hara var. graciliflorus is K （2n） =2x =24 = 16m ＋8Sm, sugges- ting that this species belongs to category 1B of Stebbins. [Conclusion] R. lophanthoides var. gerardianus is more primitive compared with R. lophanthoides Hara var. graciliflorus.
基金supported by the Key Science-Technology Project of the National '11th Five-Year Plan' of China(No.2006BAI06A15-3).
文摘A new phenylethanoid glycoside, 3-hydroxy-4-methoxy-β-phenylethoxy-O-α-L-rhamnopyranosyl-（1 → 3）-2-O-acetyl-O-β-D- glucopyranoside, named lophanthoside A, was isolated from Rabdosia lophanthoides （Buch.-Ham.ex D.Don） Hara. Its structure was determined by spectroscopic evidences.
文摘The structures of two new abietane quinones, named micranthins A and B, were determined to be 7a-methoxy-14, 16-epoxy-8, 13-abietadiene-11, 12-dione (1) and 16-acetoxy-6, 7-dehydroroyleanone (2) respectively, which were isolated from Isodon lophanthoides var. micranthus.
文摘Gas chromatography/mass spectrometry (GC MS) of the essential oil from the aerial parts of Rabdosia lophanthoides resulted in the identification of 108 compounds representing 78.120% of the oil. Hydro distillation of Rabdosia lophanthoides yielded a pale yellow oil. The compounds identified and their relative proportions are listed in Table 1 according to their order of elution on an HP 5MS capillary column. .
