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Pharmacognosy Identification of Xiaohuangcao (Dendrobium loddigesi Rolfe)
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作者 Miao ZHANG Li LI +3 位作者 Jianbei TENG Hua ZHU Zhonghua DAI Long CHEN 《Agricultural Biotechnology》 CAS 2019年第3期177-179,共3页
[Objectives]Pharmacognosy identification was performed on Xiaohuangcao ( Dendrobium loddigesi Rolfe).[Methods]The medicinal materials were identified through original plants,characters,microscopic structure and thin-l... [Objectives]Pharmacognosy identification was performed on Xiaohuangcao ( Dendrobium loddigesi Rolfe).[Methods]The medicinal materials were identified through original plants,characters,microscopic structure and thin-layer identification characteristics.[Results]D.loddigesi has obvious plant morphology,characters,microscopic structure and thin-layer identification characteristics.The stem of Xiaohuangcao is slender and cylindrical,and the surface is golden yellow;and the fiber bundles outside the vascular bundles are crescent-shaped or semi-circular in the stem transection.For the powder,crystal fiber can be observed;the vascular bundles are embedded with siliceous block cells;and there are more starch grains.In the thin layer chromatography,petroleum ether-ethyl acetate-butanone-glacial acetic acid (8.5∶ 3.5∶ 1.5∶ 5 d) was used as a developing solvent,and 10% sulfuric acid ethanol solution was used as a color developing agent.[Conclusions]The research results provide reference for the application of the medicinal material and the formulation of its related quality standards. 展开更多
关键词 DENDROBIUM loddigesi Rolfe Character IDENTIFICATION MICROSCOPIC IDENTIFICATION THIN-LAYER CHROMATOGRAPHY
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基于UPLC-MS/MS代谢组学的美花石斛与流苏石斛成分分析 被引量:2
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作者 任佳妮 黎理 +1 位作者 黄玉妹 滕建北 《现代中药研究与实践》 CAS 2023年第1期17-22,共6页
目的研究美花石斛和流苏石斛的化学成分异同,明确其特征性差异代谢物,发现共性药效的物质作用基础。方法以统一化种植管理的美花石斛和流苏石斛组培苗为试材,两种石斛的代谢物采用UPLC-MS/MS和matware数据库对二级谱图数据定性分析,从... 目的研究美花石斛和流苏石斛的化学成分异同,明确其特征性差异代谢物,发现共性药效的物质作用基础。方法以统一化种植管理的美花石斛和流苏石斛组培苗为试材,两种石斛的代谢物采用UPLC-MS/MS和matware数据库对二级谱图数据定性分析,从代谢组水平对石斛茎中的小分子化合物成分和变化进行分析,结合多元变量统计学分析方法,对石斛的代谢物进行主成分分析,通过最小二乘判别分析,以VIP>1和P<0.05为条件进行筛选和鉴定,得出2种石斛的差异代谢物,并通过KEGG数据库进行代谢通路富集分析。结果共检测出代谢物1077个,差异代谢物594个。流苏石斛相对于美花石斛分别有172个下调、422个上调代谢物,其中被KEGG注释到的差异代谢物有176个,涉及代谢通路77条,主要分布在19条途径中,其中P<0.05的有4条,分别是精氨酸和脯氨酸代谢、色氨酸代谢、ABC转运体和硫代葡萄糖苷生物合成途径。石斛的共有代谢物483个,主要包含酚酸类、游离脂肪酸类、有机酸类、糖及醇类等化合物。结论鉴定了流苏石斛和美花石斛的差异性代谢物和共有代谢物,并推测出相关代谢通路,有助于揭示两种石斛共同功效的主要成分、寻找质量标志物,从而为多物种来源中药的共同药效物质研究提供参考。 展开更多
关键词 美花石斛 流苏石斛 代谢组学 主成分分析 UPLC-MS/MS
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