In this paper,we numerically analyze the factors determining localization precision and resolution in single emitter localization-based imaging systems.While previous studies have considered a limited set of parameter...In this paper,we numerically analyze the factors determining localization precision and resolution in single emitter localization-based imaging systems.While previous studies have considered a limited set of parameters,our numerical approach incorporates additional parameters with significant reference values,yielding a more comprehensive analysis of the results.We differentiate between the effects of additive and multiplicative noise on localization precision using numerical modeling and take the influence of the sampling frequency into account,computing the optimal sampling frequency for varying resolution requirements.Leveraging a suite of derived equations,we systematically simulate and quantify how variations in these parameters influence system performance.Furthermore,we provide guidelines for optimizing signal-to-noise ratio(SNR)requirements and pixel size selection based on point spread function(PSF)width in single emitter localization-based imaging systems.This numerically driven research offers critical insights for the analysis of more complex imaging systems.展开更多
In this paper, we study the case of independent sums in multi-risk model. Assume that there exist k types of variables. The ith are denoted by （Xij,j ≥ 1）, which are i.i.d. with common density function fi（x） ∈ O...In this paper, we study the case of independent sums in multi-risk model. Assume that there exist k types of variables. The ith are denoted by （Xij,j ≥ 1）, which are i.i.d. with common density function fi（x） ∈ OR and finite mean, i =- 1,., k. We investigate local large deviations for partial sums ∑i=1^k Sni=∑i=1^k ∑j=1^ni Xij.展开更多
The national benchmarks on islands were mostly established by trigonometric leveling in Korea. This method results in inaccuracy, which is a serious problem in Geoga Grand Bridge construction work that tried to link t...The national benchmarks on islands were mostly established by trigonometric leveling in Korea. This method results in inaccuracy, which is a serious problem in Geoga Grand Bridge construction work that tried to link the mainland and the islands. The Geoga Grand Bridge （Pusan-Geoje fixed link project） was selected as the study area, a huge construction work in Korea that will connect the mainland （Pnsan） and an island （Gecje island）. However, the orthometric heights issued at benchmarks （JINH and GOFJ） were not consistent, because they did not refer to the same zero point, which would make the linking of the sections problematic. This paper introduces the precise local geoidas a vertical datum for the construction area in order to establish a consistent height system. To determine the precise local geoid for the construction area, we firstly developed a precise gravimetric geoid for Korea and its adjoining seas as a whole. This gravimetric geoid was developed by use of all available gravity data, including surface and satellite data on land and on the ocean. The gravimetrie gecid was computed by spherical fast fourier transform with modified Stokes＇ kernels. The remove-restore technique was used to eliminate the terrain effects by use of the RTM reduction and to determine the residual geoid by combining the GGM02S/EGM96 geopotential model, free-air gravity anomalies and high-resolutinn DEM data. Finally, the gravimetric model was fitted to the geoid heights obtained from GPS and tide observations （Ncps/Tiae） by least square coUocatian, to provide the final GPS-consistent local precise geoid model. The post-fit error （std. dev. ） of the final geoid to the NetS/Tide derived from GPS and tide observations was ± 2.2 cm for the construction area. We solved the height inconsistency problem by calculating the orthometric height of the benchmarks and the cnntrol points using the final geoid model. Also, the highly accurate orthometric height was estimated through the GPS/leveling technique by applying the developed local precise geoid. Therefore, the precise local geoid is expected to improve the quality of the construction procedure of the Geoga Grand Bridge.展开更多
Minimal photon fluxes(MINFLUX)nanoscopy has emerged as a transformative advancement in superresolution imaging,enabling unprecedented nanoscale observations across diverse biological scenarios.In this work,we propose,...Minimal photon fluxes(MINFLUX)nanoscopy has emerged as a transformative advancement in superresolution imaging,enabling unprecedented nanoscale observations across diverse biological scenarios.In this work,we propose,for the first time,that employing high-order vortex beams can significantly enhance the performance of MINFLUX,surpassing the limitations of the conventional MINFLUX using the first-order vortex beam.Our theoretical analysis indicates that,for standard MINFLUX,high-order vortex beams can improve the maximum localization precision by a factor corresponding to their order,which can approach a sub-nanometer scale under optimal conditions,and for raster scan MINFLUX,high-order vortex beams allow for a wider field of view while maintaining enhanced precision.These findings underscore the potential of high-order vortex beams to elevate the performance of MINFLUX,paving the way towards ultra-high resolution imaging for a broad range of applications.展开更多
Advanced fluorescence microscopy including single-molecule localization-based super-resolution imaging techniques requires bright and photostable dyes orproteins asfluorophores.The photophysical properties of fluoroph...Advanced fluorescence microscopy including single-molecule localization-based super-resolution imaging techniques requires bright and photostable dyes orproteins asfluorophores.The photophysical properties of fluorophores have been proven to be crucial for super-resolution microscopy's localization precision and imaging resolution.Fluorophores TAMRA and Atto Rho6 G,which can interact with macrocyclic host cucurbit[7]uril(CB7) to form host-vip compounds,were found to improve the fluorescence intensity and lifetimes of these dyes.We enhanced the localization precision of direct stochastic optical reconstruction microscopy(dSTORM) by introducing CB7 into the imaging buffer,and showed that the number of photons as well as localizations of both TAMRA and Atto Rho6 G increase over 2 times.展开更多
Recently,deeplearning based fingerprint localization has attracted significant interest due to its simplicity in implementation and effectiveness in complex multipath environments,especially for the Internet of Things...Recently,deeplearning based fingerprint localization has attracted significant interest due to its simplicity in implementation and effectiveness in complex multipath environments,especially for the Internet of Things(loT)devices in multiple-input multiple-output(MiMO)-orthogonal frequency-division multiplexing(OFDM)system.However,the huge amount of training data collection has become a challenge,which increases the labor burden of fingerprint localization heavily and hinders its large-scale implementation.In this paper,we propose a novel fingerprint localization system,termed as SiamResNet,which can be trained only on the radio map by contrastive self-supervised learning without the need for any other additional data.To be more specific,we first model the fingerprint localization problem as a dictionary look-up task.Subsequently,a channel fingerprint capturing the multipath angle and delay of wireless propagation is introduced,which exhibits excellent uniqueness,stability,and distinguishability.Meanwhile,we propose the corresponding data augmentation strategy to ensure data diversity when generating the training data from the radio map.Thus,the cost of data collection for training can be significantly reduced.Lastly,the Siamese architecture based SiamResNet is applied for location estimation,which can comprehensively extract the features of fingerprints and accurately compare the similarity of any fingerprint to the radio map in the representation space.The performance of the proposed localization method is validated through extensive simulations with a ray-tracing channel model,which demonstrates promising localization accuracy for our SiamResNet with reduced training costs.展开更多
基金Project supported by the National Key Research and Development Program of China(No.2022YFB3206000)the Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences(No.CI2023C009YG)the Research and Development Program of Jiaxing(No.2022AD10028)。
文摘In this paper,we numerically analyze the factors determining localization precision and resolution in single emitter localization-based imaging systems.While previous studies have considered a limited set of parameters,our numerical approach incorporates additional parameters with significant reference values,yielding a more comprehensive analysis of the results.We differentiate between the effects of additive and multiplicative noise on localization precision using numerical modeling and take the influence of the sampling frequency into account,computing the optimal sampling frequency for varying resolution requirements.Leveraging a suite of derived equations,we systematically simulate and quantify how variations in these parameters influence system performance.Furthermore,we provide guidelines for optimizing signal-to-noise ratio(SNR)requirements and pixel size selection based on point spread function(PSF)width in single emitter localization-based imaging systems.This numerically driven research offers critical insights for the analysis of more complex imaging systems.
文摘In this paper, we study the case of independent sums in multi-risk model. Assume that there exist k types of variables. The ith are denoted by （Xij,j ≥ 1）, which are i.i.d. with common density function fi（x） ∈ OR and finite mean, i =- 1,., k. We investigate local large deviations for partial sums ∑i=1^k Sni=∑i=1^k ∑j=1^ni Xij.
文摘The national benchmarks on islands were mostly established by trigonometric leveling in Korea. This method results in inaccuracy, which is a serious problem in Geoga Grand Bridge construction work that tried to link the mainland and the islands. The Geoga Grand Bridge （Pusan-Geoje fixed link project） was selected as the study area, a huge construction work in Korea that will connect the mainland （Pnsan） and an island （Gecje island）. However, the orthometric heights issued at benchmarks （JINH and GOFJ） were not consistent, because they did not refer to the same zero point, which would make the linking of the sections problematic. This paper introduces the precise local geoidas a vertical datum for the construction area in order to establish a consistent height system. To determine the precise local geoid for the construction area, we firstly developed a precise gravimetric geoid for Korea and its adjoining seas as a whole. This gravimetric geoid was developed by use of all available gravity data, including surface and satellite data on land and on the ocean. The gravimetrie gecid was computed by spherical fast fourier transform with modified Stokes＇ kernels. The remove-restore technique was used to eliminate the terrain effects by use of the RTM reduction and to determine the residual geoid by combining the GGM02S/EGM96 geopotential model, free-air gravity anomalies and high-resolutinn DEM data. Finally, the gravimetric model was fitted to the geoid heights obtained from GPS and tide observations （Ncps/Tiae） by least square coUocatian, to provide the final GPS-consistent local precise geoid model. The post-fit error （std. dev. ） of the final geoid to the NetS/Tide derived from GPS and tide observations was ± 2.2 cm for the construction area. We solved the height inconsistency problem by calculating the orthometric height of the benchmarks and the cnntrol points using the final geoid model. Also, the highly accurate orthometric height was estimated through the GPS/leveling technique by applying the developed local precise geoid. Therefore, the precise local geoid is expected to improve the quality of the construction procedure of the Geoga Grand Bridge.
基金supported in part by the Academic Research Fund(AcRF)-Tier 2(A-8000117-01-00)and Tier 1(A-8003279-00-00)from the Ministry of Education(MOE)of Singapore,Science and Technology Project of Jiangsu Province(BZ2022056),NUS(Suzhou)Research Institute/Biomedical and Health Technology Platform,2024 Tsinghua-NUS Joint Research Initiative Fund(A-8002557-00-00)the National Medical Research Council(NMRC)(A-0009502-01-00,and A-8001143-00-00),Singapore.
文摘Minimal photon fluxes(MINFLUX)nanoscopy has emerged as a transformative advancement in superresolution imaging,enabling unprecedented nanoscale observations across diverse biological scenarios.In this work,we propose,for the first time,that employing high-order vortex beams can significantly enhance the performance of MINFLUX,surpassing the limitations of the conventional MINFLUX using the first-order vortex beam.Our theoretical analysis indicates that,for standard MINFLUX,high-order vortex beams can improve the maximum localization precision by a factor corresponding to their order,which can approach a sub-nanometer scale under optimal conditions,and for raster scan MINFLUX,high-order vortex beams allow for a wider field of view while maintaining enhanced precision.These findings underscore the potential of high-order vortex beams to elevate the performance of MINFLUX,paving the way towards ultra-high resolution imaging for a broad range of applications.
基金supported by the National Natural Science Foundation of China(31330082,21373200,21525314)the Instrument Developing Project of the Chinese Academy of Sciences(YZ201455)
文摘Advanced fluorescence microscopy including single-molecule localization-based super-resolution imaging techniques requires bright and photostable dyes orproteins asfluorophores.The photophysical properties of fluorophores have been proven to be crucial for super-resolution microscopy's localization precision and imaging resolution.Fluorophores TAMRA and Atto Rho6 G,which can interact with macrocyclic host cucurbit[7]uril(CB7) to form host-vip compounds,were found to improve the fluorescence intensity and lifetimes of these dyes.We enhanced the localization precision of direct stochastic optical reconstruction microscopy(dSTORM) by introducing CB7 into the imaging buffer,and showed that the number of photons as well as localizations of both TAMRA and Atto Rho6 G increase over 2 times.
文摘Recently,deeplearning based fingerprint localization has attracted significant interest due to its simplicity in implementation and effectiveness in complex multipath environments,especially for the Internet of Things(loT)devices in multiple-input multiple-output(MiMO)-orthogonal frequency-division multiplexing(OFDM)system.However,the huge amount of training data collection has become a challenge,which increases the labor burden of fingerprint localization heavily and hinders its large-scale implementation.In this paper,we propose a novel fingerprint localization system,termed as SiamResNet,which can be trained only on the radio map by contrastive self-supervised learning without the need for any other additional data.To be more specific,we first model the fingerprint localization problem as a dictionary look-up task.Subsequently,a channel fingerprint capturing the multipath angle and delay of wireless propagation is introduced,which exhibits excellent uniqueness,stability,and distinguishability.Meanwhile,we propose the corresponding data augmentation strategy to ensure data diversity when generating the training data from the radio map.Thus,the cost of data collection for training can be significantly reduced.Lastly,the Siamese architecture based SiamResNet is applied for location estimation,which can comprehensively extract the features of fingerprints and accurately compare the similarity of any fingerprint to the radio map in the representation space.The performance of the proposed localization method is validated through extensive simulations with a ray-tracing channel model,which demonstrates promising localization accuracy for our SiamResNet with reduced training costs.
