For more than a decade numerous evidence has been reported on the mechanisms of toxicity of α-synuclein（αS） oligomers and aggregates in α-synucleinopathies.These species were thought to form freely in the cytopla...For more than a decade numerous evidence has been reported on the mechanisms of toxicity of α-synuclein（αS） oligomers and aggregates in α-synucleinopathies.These species were thought to form freely in the cytoplasm but recent reports of αS multimer conformations when bound to synaptic vesicles in physiological conditions,have raised the question about where αS aggregation initiates.In this review we focus on recent literature regarding the impact on membrane binding and subcellular localization of αS toxic species to understand how regular cellular function of αS contributes to pathology.Notably αS has been reported to mainly associate with specific membranes in neurons such as those of synaptic vesicles,ER/Golgi and the mitochondria,while toxic species of αS have been shown to inhibit,among others,neurotransmission,protein trafficking and mitochondrial function.Strategies interfering with αS membrane binding have shown to improve αS-driven toxicity in worms and in mice.Thus,a selective membrane binding that would result in a specific subcellular localization could be the key to understand how aggregation and pathology evolves,pointing out to αS functions that are primarily affected before onset of irreversible damage.展开更多
Few-shot learning has emerged as a crucial technique for coral species classification,addressing the challenge of limited labeled data in underwater environments.This study introduces an optimized few-shot learning mo...Few-shot learning has emerged as a crucial technique for coral species classification,addressing the challenge of limited labeled data in underwater environments.This study introduces an optimized few-shot learning model that enhances classification accuracy while minimizing reliance on extensive data collection.The proposed model integrates a hybrid similarity measure combining Euclidean distance and cosine similarity,effectively capturing both feature magnitude and directional relationships.This approach achieves a notable accuracy of 71.8%under a 5-way 5-shot evaluation,outperforming state-of-the-art models such as Prototypical Networks,FEAT,and ESPT by up to 10%.Notably,the model demonstrates high precision in classifying Siderastreidae(87.52%)and Fungiidae(88.95%),underscoring its effectiveness in distinguishing subtle morphological differences.To further enhance performance,we incorporate a self-supervised learning mechanism based on contrastive learning,enabling the model to extract robust representations by leveraging local structural patterns in corals.This enhancement significantly improves classification accuracy,particularly for species with high intra-class variation,leading to an overall accuracy of 76.52%under a 5-way 10-shot evaluation.Additionally,the model exploits the repetitive structures inherent in corals,introducing a local feature aggregation strategy that refines classification through spatial information integration.Beyond its technical contributions,this study presents a scalable and efficient approach for automated coral reef monitoring,reducing annotation costs while maintaining high classification accuracy.By improving few-shot learning performance in underwater environments,our model enhances monitoring accuracy by up to 15%compared to traditional methods,offering a practical solution for large-scale coral conservation efforts.展开更多
As an indispensable part of identity authentication,offline writer identification plays a notable role in biology,forensics,and historical document analysis.However,identifying handwriting efficiently,stably,and quick...As an indispensable part of identity authentication,offline writer identification plays a notable role in biology,forensics,and historical document analysis.However,identifying handwriting efficiently,stably,and quickly is still challenging due to the method of extracting and processing handwriting features.In this paper,we propose an efficient system to identify writers through handwritten images,which integrates local and global features from similar handwritten images.The local features are modeled by effective aggregate processing,and global features are extracted through transfer learning.Specifically,the proposed system employs a pre-trained Residual Network to mine the relationship between large image sets and specific handwritten images,while the vector of locally aggregated descriptors with double power normalization is employed in aggregating local and global features.Moreover,handwritten image segmentation,preprocessing,enhancement,optimization of neural network architecture,and normalization for local and global features are exploited,significantly improving system performance.The proposed system is evaluated on Computer Vision Lab(CVL)datasets and the International Conference on Document Analysis and Recognition(ICDAR)2013 datasets.The results show that it represents good generalizability and achieves state-of-the-art performance.Furthermore,the system performs better when training complete handwriting patches with the normalization method.The experimental result indicates that it’s significant to segment handwriting reasonably while dealing with handwriting overlap,which reduces visual burstiness.展开更多
基金supported by the Italian Ministry of University and Research(MIUR) through the Career Reintegration grant scheme(RLM Program for Young Researcher)and from Scuola Normale Superiore
文摘For more than a decade numerous evidence has been reported on the mechanisms of toxicity of α-synuclein（αS） oligomers and aggregates in α-synucleinopathies.These species were thought to form freely in the cytoplasm but recent reports of αS multimer conformations when bound to synaptic vesicles in physiological conditions,have raised the question about where αS aggregation initiates.In this review we focus on recent literature regarding the impact on membrane binding and subcellular localization of αS toxic species to understand how regular cellular function of αS contributes to pathology.Notably αS has been reported to mainly associate with specific membranes in neurons such as those of synaptic vesicles,ER/Golgi and the mitochondria,while toxic species of αS have been shown to inhibit,among others,neurotransmission,protein trafficking and mitochondrial function.Strategies interfering with αS membrane binding have shown to improve αS-driven toxicity in worms and in mice.Thus,a selective membrane binding that would result in a specific subcellular localization could be the key to understand how aggregation and pathology evolves,pointing out to αS functions that are primarily affected before onset of irreversible damage.
基金funded by theNational Science and TechnologyCouncil(NSTC),Taiwan,under grant numbers NSTC 112-2634-F-019-001 and NSTC 113-2634-F-A49-007.
文摘Few-shot learning has emerged as a crucial technique for coral species classification,addressing the challenge of limited labeled data in underwater environments.This study introduces an optimized few-shot learning model that enhances classification accuracy while minimizing reliance on extensive data collection.The proposed model integrates a hybrid similarity measure combining Euclidean distance and cosine similarity,effectively capturing both feature magnitude and directional relationships.This approach achieves a notable accuracy of 71.8%under a 5-way 5-shot evaluation,outperforming state-of-the-art models such as Prototypical Networks,FEAT,and ESPT by up to 10%.Notably,the model demonstrates high precision in classifying Siderastreidae(87.52%)and Fungiidae(88.95%),underscoring its effectiveness in distinguishing subtle morphological differences.To further enhance performance,we incorporate a self-supervised learning mechanism based on contrastive learning,enabling the model to extract robust representations by leveraging local structural patterns in corals.This enhancement significantly improves classification accuracy,particularly for species with high intra-class variation,leading to an overall accuracy of 76.52%under a 5-way 10-shot evaluation.Additionally,the model exploits the repetitive structures inherent in corals,introducing a local feature aggregation strategy that refines classification through spatial information integration.Beyond its technical contributions,this study presents a scalable and efficient approach for automated coral reef monitoring,reducing annotation costs while maintaining high classification accuracy.By improving few-shot learning performance in underwater environments,our model enhances monitoring accuracy by up to 15%compared to traditional methods,offering a practical solution for large-scale coral conservation efforts.
基金supported in part by the Postgraduate Research&Practice Innovation Program of Jiangsu Province under Grant KYCX 20_0758in part by the Science and Technology Research Project of Jiangsu Public Security Department under Grant 2020KX005+1 种基金in part by the General Project of Philosophy and Social Science Research in Colleges and Universities in Jiangsu Province under Grant 2022SJYB0473in part by“Cyberspace Security”Construction Project of Jiangsu Provincial Key Discipline during the“14th Five Year Plan”.
文摘As an indispensable part of identity authentication,offline writer identification plays a notable role in biology,forensics,and historical document analysis.However,identifying handwriting efficiently,stably,and quickly is still challenging due to the method of extracting and processing handwriting features.In this paper,we propose an efficient system to identify writers through handwritten images,which integrates local and global features from similar handwritten images.The local features are modeled by effective aggregate processing,and global features are extracted through transfer learning.Specifically,the proposed system employs a pre-trained Residual Network to mine the relationship between large image sets and specific handwritten images,while the vector of locally aggregated descriptors with double power normalization is employed in aggregating local and global features.Moreover,handwritten image segmentation,preprocessing,enhancement,optimization of neural network architecture,and normalization for local and global features are exploited,significantly improving system performance.The proposed system is evaluated on Computer Vision Lab(CVL)datasets and the International Conference on Document Analysis and Recognition(ICDAR)2013 datasets.The results show that it represents good generalizability and achieves state-of-the-art performance.Furthermore,the system performs better when training complete handwriting patches with the normalization method.The experimental result indicates that it’s significant to segment handwriting reasonably while dealing with handwriting overlap,which reduces visual burstiness.
