The development of 3D cell culturing toward labor saving and versatility is highly desired.Here,we propose a platform consisting of a multiwell plate and liquidmarbles(LMs).The inner walls of the plate are covered wit...The development of 3D cell culturing toward labor saving and versatility is highly desired.Here,we propose a platform consisting of a multiwell plate and liquidmarbles(LMs).The inner walls of the plate are covered with particle-detachable superhydrophobic coatings that serve as both the substrates and particle sources for LM production.A produced LM,which serves as aminireactor for the 3D culture,features a monolayer nanoparticulate shell and naked-droplet-like transparency.The LM-inplate platform provides a double-superhydrophobic environment that increases the stability of the 3D culture and reduces the necessary operational cautions.In addition,both cell observation and high-throughput applications can be conducted in situ,owing to the high LM transparency and the multiwell structure,respectively.This platform integrates the advantages of naked droplets(transparent and clean),LMs(stable nonwetting),and multiwell plates(high-throughput capability)and thus is promising for labor-saving and versatile 3D culturing.展开更多
基金National Natural Science Foundation of China,Grant/Award Numbers:52473323,11974280Projects of Interdisciplinary,NWPU,Grant/Award Number:0202022GH0306+2 种基金Natural Science Basic Research Plan in Shaanxi Province of China,Grant/Award Number:2024JC-YBMS-605Guangdong Basic and Applied Basic Research Foundation,Grant/Award Number:2023A1515030047National Undergraduate Training Programs for Innovation and Entrepreneurship,Grant/Award Number:S202210699549。
文摘The development of 3D cell culturing toward labor saving and versatility is highly desired.Here,we propose a platform consisting of a multiwell plate and liquidmarbles(LMs).The inner walls of the plate are covered with particle-detachable superhydrophobic coatings that serve as both the substrates and particle sources for LM production.A produced LM,which serves as aminireactor for the 3D culture,features a monolayer nanoparticulate shell and naked-droplet-like transparency.The LM-inplate platform provides a double-superhydrophobic environment that increases the stability of the 3D culture and reduces the necessary operational cautions.In addition,both cell observation and high-throughput applications can be conducted in situ,owing to the high LM transparency and the multiwell structure,respectively.This platform integrates the advantages of naked droplets(transparent and clean),LMs(stable nonwetting),and multiwell plates(high-throughput capability)and thus is promising for labor-saving and versatile 3D culturing.
