目的检测人类细胞表面糖蛋白2(SCUBE2)在乳腺癌组织中的表达情况,探讨SCUBE2表达与乳腺癌患者临床病理特征及乳腺癌关键基因的相关性。方法应用液相芯片基因检测技术检测152例乳腺癌组织样本SCUBE2、Bcl-2、Survivin及PTEN基因m RNA表达...目的检测人类细胞表面糖蛋白2(SCUBE2)在乳腺癌组织中的表达情况,探讨SCUBE2表达与乳腺癌患者临床病理特征及乳腺癌关键基因的相关性。方法应用液相芯片基因检测技术检测152例乳腺癌组织样本SCUBE2、Bcl-2、Survivin及PTEN基因m RNA表达,分析上述指标与乳腺癌患者临床病理学资料及它们之间的相关性。结果在乳腺癌中SCUBE2 m RNA的表达与ER、PR及Ki67状态密切相关(P<0.05);进一步分析结果显示,中—高表达SCUBE2的患者Ki67活跃程度明显低于低表达SCUBE2的患者(P<0.05)。Survivin m RNA的表达与Ki67状态密切相关(P<0.05);PTEN m RNA表达与ER及PR状态密切相关(P<0.05);Bcl-2 m RNA表达与淋巴结转移、ER、PR及Ki67状态密切相关(P<0.05)。Spearsman等级相关法分析表明,SCUBE2表达与Bcl-2及PTEN表达呈正相关(r=0.434、0.348,P<0.01),而SCUBE2表达与Survivin表达呈负相关(r=-0.249,P<0.01)。结论在乳腺癌中SCUBE2表达与肿瘤增殖密切相关,可能是乳腺癌重要预后指标。展开更多
Background Personalized medicine becomes essential in lung cancer treatment, however lung-cancer-related gene expression profiles in Chinese patients remain unknown. In this study, the correlation of gene expression p...Background Personalized medicine becomes essential in lung cancer treatment, however lung-cancer-related gene expression profiles in Chinese patients remain unknown. In this study, the correlation of gene expression profiles and clinical characteristics in non-small-cell lung cancer （NSCLC） was investigated. Methods Seventy-six Chinese patients with NSCLC were enrolled in the study to investigate mRNA expression profiles of excision repair cross complement group 1 （ERCC1）, thymidylate synthetase （TYMS）, ribonucleotide reductase （RRM1）, class Ill 13-tubulin （TUBB3）, and epidermal growth factor receptor （EGFR） genes and their correlation with patient clinical characteristics. A novel liquidchip technology was used to detect mRNA expression levels in formalin fixed paraffin embedded tumor pathology samples. The relationships between gene expression and clinical characteristics were assessed using the Mann-Whitney test. Results ERCC1 mRNA levels were higher in tumors from patients with metastatic disease than patients with non- metastatic disease （P=-0.021）, and higher in adenocarcinomas than squamous cell carcinomas （P=0.006）. Increased TUBB3 mRNA expression levels were found in patients with performance status （PS） 1 in comparison with PS 0 （P=0.049）, with poorly differentiated tumors in comparison with tumors that were moderately and well differentiated （P 〈0.000 1）, and with advanced stage in comparison with early stage disease （P 〈0.000 1）. Conclusions ERCC1 mRNA levels were higher in metastatic adenocarcinoma NSCLC; TUBB3 mRNA levels were significantly higher in poorly differentiated tumors and in advanced stage NSCLC, which indicates the poor prognosis.展开更多
文摘目的检测人类细胞表面糖蛋白2(SCUBE2)在乳腺癌组织中的表达情况,探讨SCUBE2表达与乳腺癌患者临床病理特征及乳腺癌关键基因的相关性。方法应用液相芯片基因检测技术检测152例乳腺癌组织样本SCUBE2、Bcl-2、Survivin及PTEN基因m RNA表达,分析上述指标与乳腺癌患者临床病理学资料及它们之间的相关性。结果在乳腺癌中SCUBE2 m RNA的表达与ER、PR及Ki67状态密切相关(P<0.05);进一步分析结果显示,中—高表达SCUBE2的患者Ki67活跃程度明显低于低表达SCUBE2的患者(P<0.05)。Survivin m RNA的表达与Ki67状态密切相关(P<0.05);PTEN m RNA表达与ER及PR状态密切相关(P<0.05);Bcl-2 m RNA表达与淋巴结转移、ER、PR及Ki67状态密切相关(P<0.05)。Spearsman等级相关法分析表明,SCUBE2表达与Bcl-2及PTEN表达呈正相关(r=0.434、0.348,P<0.01),而SCUBE2表达与Survivin表达呈负相关(r=-0.249,P<0.01)。结论在乳腺癌中SCUBE2表达与肿瘤增殖密切相关,可能是乳腺癌重要预后指标。
文摘Background Personalized medicine becomes essential in lung cancer treatment, however lung-cancer-related gene expression profiles in Chinese patients remain unknown. In this study, the correlation of gene expression profiles and clinical characteristics in non-small-cell lung cancer （NSCLC） was investigated. Methods Seventy-six Chinese patients with NSCLC were enrolled in the study to investigate mRNA expression profiles of excision repair cross complement group 1 （ERCC1）, thymidylate synthetase （TYMS）, ribonucleotide reductase （RRM1）, class Ill 13-tubulin （TUBB3）, and epidermal growth factor receptor （EGFR） genes and their correlation with patient clinical characteristics. A novel liquidchip technology was used to detect mRNA expression levels in formalin fixed paraffin embedded tumor pathology samples. The relationships between gene expression and clinical characteristics were assessed using the Mann-Whitney test. Results ERCC1 mRNA levels were higher in tumors from patients with metastatic disease than patients with non- metastatic disease （P=-0.021）, and higher in adenocarcinomas than squamous cell carcinomas （P=0.006）. Increased TUBB3 mRNA expression levels were found in patients with performance status （PS） 1 in comparison with PS 0 （P=0.049）, with poorly differentiated tumors in comparison with tumors that were moderately and well differentiated （P 〈0.000 1）, and with advanced stage in comparison with early stage disease （P 〈0.000 1）. Conclusions ERCC1 mRNA levels were higher in metastatic adenocarcinoma NSCLC; TUBB3 mRNA levels were significantly higher in poorly differentiated tumors and in advanced stage NSCLC, which indicates the poor prognosis.
