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Fermented liquid feed for pigs: an ancient technique for the future 被引量:88
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作者 Joris AM Missotten Joris Michiels +1 位作者 Jeroen Degroote Stefaan De Smet 《Journal of Animal Science and Biotechnology》 CAS CSCD 2016年第1期1-9,共9页
Fermented liquid feed is feed that has been mixed with water at a ratio ranging from 1:1.5 to 1:4. By mixing with water lactic acid bacteria and yeasts naturally occurring in the feed proliferate and produce lactic ... Fermented liquid feed is feed that has been mixed with water at a ratio ranging from 1:1.5 to 1:4. By mixing with water lactic acid bacteria and yeasts naturally occurring in the feed proliferate and produce lactic acid, acetic acid and ethano which reduces the pH of the mixture. This reduction in pH inhibits pathogenic organisms from developing in the feed. In addition, when this low pH mixture is fed, it reduces the pH in the stomach of pigs and prevents the proliferation of pathogens such as coliforms and Salmonella in the gastrointestinal tract. For piglets, the use of fermented liquid feed offers the possibility of simultaneously providing feed and water, which may facilitate an easier transition from sow's milk to solid feed. Secondly, offering properly produced fermented liquid feed may strengthen the role of the stomach as the first line of defense against possible pathogenic infections by lowering the pH in the gastrointestinal tract thereby helping to exclude enteropathogens. Finally, feeding fermented liquid feed to pigs has been shown to improve the performance of suckling pigs, weaner pigs and growing-finishing pigs. In this review, current knowledge about the use of fermented liquid feed in pig diets will be discussed. This will include a discussion of the desirable properties of fermented liquid feed and factors affecting fermentation. In addition, advantages and disadvantages of fermented liquid feed will be discussed including its effects on gastrointestinal health, intestinal pH and the types of bacteria found in the gastrointestinal tract as well as the effects of fermented liquid feeds on pig performance. 展开更多
关键词 Fermented liquid feed Lactobacillus spp PIGS PROBIOTICS Yeasts
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Effects of liquid feeding of corn condensed distiller's solubles and whole stillage on growth performance, carcass characteristics, and sensory traits of pigs
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作者 Xiaojian Yang Carissa Nath +2 位作者 Alan Doering John Goihl Samuel Kofi Baidoo 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2017年第2期467-477,共11页
Background: The immense growth in global bioethanol production has greatly increased the supply of by-products such as whole stillage and condensed distiller's solubles, which could be potentially used for animal fe... Background: The immense growth in global bioethanol production has greatly increased the supply of by-products such as whole stillage and condensed distiller's solubles, which could be potentially used for animal feeding. The objective of this study was to investigate effects of liquid feeding high levels of corn condensed distiller's solubles(CCDS) and whole stillage(CWS) on growth performance, carcass characteristics, belly firmness and meat sensory traits of pigs.Methods: A total of 256 pigs were blocked by sex and initial BW(13.5 ± 2.5 kg), and pens of pigs(8 pigs/pen) were randomly allocated to 1 of 4 dietary treatments(8 pens/treatment): 1) corn-soybean meal based diet as control, 2) 25%CWS + 5% CCDS, 3) 19.5% CWS + 10.5% CCDS, and 4) 19.5, 26, and 32.5% CWS + 10.5, 14, and 17.5% CCDS in phases 1(28 d), 2(38 d), and 3(60 d), respectively. Inclusion levels of CCDS and CWS for Treatments 1, 2, and 3 were fixed during all the three phases of the experiment. Inclusion levels of CWS and CCDS were on 88% dry matter basis. The liquid feeding system delivered feed from the mixing tank to feed troughs by high-pressure air, had sensors inside feed troughs, and recorded daily feed intake on the basis of a reference feed intake curve. The pigs were fed 5 to 10 times per day with increasing frequency during the experiment.Results: Control pigs had greater(P 0.10) dressing percentage, loin muscle depth, and lean percentage were observed among the four treatments. Inclusion of CWS and CCDS reduced(P 0.10) the overall like,flavor, tenderness and juiciness of loin chops when compared with the control group.Conclusion: In conclusion, our results indicate that including 30–50% of a mixture of whole stillage and condensed distiller's solubles in the growing-finishing diets may reduce growth performance, carcass weight and belly firmness, but does not affect pork sensory traits. 展开更多
关键词 Carcass Condensed distiller's solubles liquid feeding Performance Pig Whole stillage
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Pyrosequencing investigation into the influence of Cu^(2+),Zn^(2+),Fe^(2+)and I^- mixtures on fungal diversity and toxigenic fungal growth in a fermented liquid feed 被引量:1
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作者 Yuyong He Zhiyu Chen +2 位作者 Hong Wen Wei Lu Huadong Wu 《Animal Nutrition》 SCIE 2016年第1期51-56,共6页
A L_9(3~4) orthogonal experiment was conducted to evaluate the influence of 9 mixtures which consisted of Cu^(2+), Zn^(2+), Fe^(2+) and I^- ions at different ion concentrations on fungal diversity and toxigenic fungal... A L_9(3~4) orthogonal experiment was conducted to evaluate the influence of 9 mixtures which consisted of Cu^(2+), Zn^(2+), Fe^(2+) and I^- ions at different ion concentrations on fungal diversity and toxigenic fungal growth in a Bacillus subtilis-fermented liquid feed(FLF) using pyrosequencing. The maximal Chao estimator and Shannon index were achieved in the FLF with a mixture of Cu^(2+)(200 mg/kg), Zn^(2+)(160 mg/kg),Fe^(2+)(150 mg/kg) and I^-(2.4 mg/kg). The minimal relative abundance of Aspergillus was achieved when a mixture of Cu^(2+)(200 mg/kg),Zn^(2+), Fe^(2+) and I^-was added to the FLF. Compared with Zn^(2+), Fe^(2+) and I^-,Cu^(2+) was the most important ion in inhibitingAspergillus growth. Adding Zn^(2+)(160 mg/kg), Cu^(2+), Fe^(2+) and I^- to the FLF minimized the relative abundance ofFusarium. It was Zn^(2+) instead of Cu^(2+) played a critical role in suppressing the growth of Fusarium. The proper use of the mixture of Cu^(2+), Zn^(2+), Fe^(2+) and I^-in FLF contributes to inhibit the growth of mycotoxin-producing fungi during storage. The new findings of this study help farmers properly use the mixture of Cu^(2+),Zn^(2+), Fe^(2+) and I^- to inhibit the growth of mycotoxinproducing fungi in the production of high quality FLF and alleviate mycotoxins damages to animals and humans. 展开更多
关键词 Trace elements Fungal growth Mixture of ions Fermented liquid feed PYROSEQUENCING
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Energy Saving on Distribution of Liquid Animal Feeds at Pigsty Farms
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作者 Ivan Ivanovich Girutski Andrei Grigorievich Senkov Natalja Mikhailovna Matsveichuk 《Journal of Agricultural Science and Technology(A)》 2015年第7期626-631,共6页
This paper focused on the task of reducing power consumption and improving energy efficiency in the technological process of distribution liquid feeds in a pigsty. The liquid feeds in a pigsty are distributed to the s... This paper focused on the task of reducing power consumption and improving energy efficiency in the technological process of distribution liquid feeds in a pigsty. The liquid feeds in a pigsty are distributed to the stables via pipelines. A centrifugal pump driven by an electric motor is used to supply the liquid feed and to move it through the pipeline. The electric motor is the major electric energy consumer in the process. As shown by experimental data and as following the theory, there is a non-linear relation between the electric motor power, the pump throughput and feed humidity. The purpose of the paper is to calculate such an optimal value of the electric motor power that provides the minimal energy consumption on the process of feed distribution. The problem is solved by the Lagrange multiplier method of nonlinear mathematical programming, taking into account geometrical parameters of the pipeline, humidity and portions volumes of distributed feed. The energy saving effect is estimated and reducing power consumption is shown. 展开更多
关键词 AUTOMATION energy saving pigs feeding liquid feed distribution.
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Restricted Daily Feeding of Liquid Meal Reduces the Motivation of Rats to Get Food
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作者 Toshihiro Yoshihara Yasutaka Yawaka 《Journal of Behavioral and Brain Science》 2015年第4期130-136,共7页
To corroborate the hypothesis that mastication affects the motivation to get food, we examined the effects of dietary consistency on the development of meal anticipation in locomotor activity of rats subjected to rest... To corroborate the hypothesis that mastication affects the motivation to get food, we examined the effects of dietary consistency on the development of meal anticipation in locomotor activity of rats subjected to restricted daily feeding (RF), in which food supply was restricted to a fixed time each day. RF of both liquid and solid meals produced anticipatory locomotor activity. However, the strength of the activity in rats subjected to RF of liquid meal was lower than that in rats subjected to RF of solid meal. These results suggest that the feeding-associated circadian oscillation that developed by feeding with liquid meal is less potent than that in the case of feeding with solid meal. Furthermore, mastication for digesting solid meal might be one of the factors that determine the strength of the feeding-associated circadian oscillation. 展开更多
关键词 liquid MEAL LOCOMOTOR Activity RESTRICTED feedING MASTICATION Rat
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First Systematic Testing Platform for Pressurization Feed System Developed for Liquid Rocket Propellant in China
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作者 Zhang Yi Beijing Aerospace System Engineering Institute of CALT 《Aerospace China》 2011年第3期-,共1页
Beijing Aerospace System Engineering Institute of China Academy of Launch Vehicle Technology (CALT) declared recently that theinstitute has set up a laboratory whichwould operate a newly
关键词 CALT feed First Systematic Testing Platform for Pressurization feed System Developed for liquid Rocket Propellant in China
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Synthesis of Iron Nanoparticles by Thermal Decomposition of Diironnonacarbonyl in Ionic Liquid and Their Potential Use as Nanotracers for Mixer Studies in Liquids Feeds
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作者 Irina Irgibaeva Nikolay Barashkov +4 位作者 Tamara Sakhno Artur Mantel Svetlana Mendigaliyeva Irina Barashkova Yuriy Sakhno 《Advances in Chemical Engineering and Science》 2020年第3期201-209,共9页
Iron nanoparticles with dynamic light scattering median diameter around 10 nm have been prepared by thermal decomposition under a nitrogen atmosphere from diironnonacarbonyl (DINC) dissolved in n-butyl-3-methylimidazo... Iron nanoparticles with dynamic light scattering median diameter around 10 nm have been prepared by thermal decomposition under a nitrogen atmosphere from diironnonacarbonyl (DINC) dissolved in n-butyl-3-methylimidazolium tetrafluoroborate (BMIMBF). The effect of temperature changes in the range of 170<span style="font-family:Verdana, Helvetica, Arial;white-space:normal;background-color:#FFFFFF;">&#176</span>C - 200<span style="font-family:Verdana, Helvetica, Arial;white-space:normal;background-color:#FFFFFF;">&#176</span>C and changes in concentration of DINC in BMIMBF in the range of 0.1% - 0.9% on the properties of obtained iron nanoparticles has been investigated. The stable dispersion of iron nanoparticle in ethanol has been prepared after separation of nanoparticles from ionic liquid by centrifugation following by their re-dispersion in ethanol. The possibility of quantitative analysis of iron content in ethanol dispersion by deposition of ferromagnetic nanoparticles on the surface of plastic-protected neodymium magnet, dissolution of iron in hydrochloric acid and addition of ammonium thiocyanate solution following by spectrophotometric determination of iron cations at wavelength of 490 nm has been investigated. The feasibility of using the same approach in case of addition of ethanol dispersion of iron nanoparticles to the liquid animal feeds for evaluation of efficiency of their mixing has been discussed. 展开更多
关键词 Ionic liquids Magnetic Nanoparticles SURFACTANT Mixing Performance Ani-mal feed
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液态饲喂发酵酱酒糟和谷物酒糟糖浆梯度替代日粮对杜长大育肥猪生长性能、胴体性状及肉品质的影响
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作者 林禹行 夏杨志航 +4 位作者 梁雯静 林柏羽 张文辉 马晓阳 沈水宝 《畜牧与饲料科学》 2026年第1期52-60,共9页
[目的]研究液态饲喂发酵酱酒糟和谷物酒糟糖浆梯度替代日粮对杜长大育肥猪生长性能、胴体性状及肉品质的影响,旨在为将其高效、安全地应用于生猪养殖提供数据支持和实践方案。[方法]选80头健康的“杜×长×大”阉割育肥猪随机分... [目的]研究液态饲喂发酵酱酒糟和谷物酒糟糖浆梯度替代日粮对杜长大育肥猪生长性能、胴体性状及肉品质的影响,旨在为将其高效、安全地应用于生猪养殖提供数据支持和实践方案。[方法]选80头健康的“杜×长×大”阉割育肥猪随机分为2组,每组包含4个重复(即4个独立的饲养单元),每个重复10头猪。试验周期77 d,分前期(1~49 d)和后期(50~77 d)两阶段实施梯度替代饲喂:对照组2个时期均使用基础日粮,前期试验组用6%发酵酱酒糟+6%谷物酒糟糖浆等量替代基础饲粮,后期替代比例调整为9%发酵酱酒糟+8%谷物酒糟糖浆,全程采用液态饲喂系统。试验结束后测定杜长大育肥猪生长性能指标、胴体性状指标、器官指数及肉品质指标。[结果]与对照组相比,试验组在试验全期的平均日增重显著(P<0.05)升高,料重比极显著(P<0.01)下降;试验组的油脂率显著(P<0.05)升高,瘦肉率显著(P<0.05)下降,眼肌面积极显著(P<0.01)下降,其余胴体性状无显著差异(P>0.05);试验组器官指数、肉品质均无显著差异(P>0.05)。[结论]在该试验条件下,采用梯度比例发酵酱酒糟与谷物酒糟糖浆替代基础饲粮对杜长大育肥猪的生长性能、胴体性状、器官指数以及肉品质的影响均无负面影响,且可改善育肥猪的生长性能,但需注意瘦肉率下降的问题。 展开更多
关键词 发酵酱酒糟 酒糟糖浆 液态饲喂 杜长大育肥猪 生长性能 胴体性状 肉品质
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高频脉冲振动铸造技术对铸钢件质量的影响机制与工艺优化研究
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作者 马娅玲 王才加尚 +1 位作者 王建鹏 高进喜 《中国铸造装备与技术》 2026年第1期28-35,共8页
本研究通过系统试验探究高频脉冲振动对铸钢金属液流动性、补缩性及缺陷抑制的作用机制。采用螺旋试样法、阶梯试样法及阀门小件试验,结合金相分析、力学性能测试和CAE模拟,揭示了高频脉冲振动的作用机制。试验结果表明:在铸造过程中实... 本研究通过系统试验探究高频脉冲振动对铸钢金属液流动性、补缩性及缺陷抑制的作用机制。采用螺旋试样法、阶梯试样法及阀门小件试验,结合金相分析、力学性能测试和CAE模拟,揭示了高频脉冲振动的作用机制。试验结果表明:在铸造过程中实施边浇边振工艺,能够显著提升金属液的流动性能,有效降低气孔率,大幅减小缩松体积,并明显提高铸件的抗拉强度及硬度。本研究进一步证实,高频振动通过高频振动力波促使气泡逸出、细化等轴晶以及延长补缩通道等关键作用,成为提升铸件综合质量的高效可行手段。 展开更多
关键词 高频脉冲振动 金属液流动性、补缩性 晶粒细化 缩松控制
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QuEChERS-超高效液相色谱-串联质谱法同时测定饲料中6类21种兽药
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作者 朱万燕 王金花 +4 位作者 倪永付 张伟 徐豪 徐久飞 张鸿伟 《分析试验室》 北大核心 2026年第1期142-148,共7页
建立了一种同时测定饲料中6类21种兽药的QuEChERS-超高效液相色谱-串联质谱(UHPLC-MS/MS)法。试样经水-1%氨化乙腈(4∶6,V/V)提取,饱和乙酸铅溶液沉淀蛋白,氯化钠、 C18和PSA净化,以XBridge peptide BEH C18(100 mm×2.1 mm,3.5μm... 建立了一种同时测定饲料中6类21种兽药的QuEChERS-超高效液相色谱-串联质谱(UHPLC-MS/MS)法。试样经水-1%氨化乙腈(4∶6,V/V)提取,饱和乙酸铅溶液沉淀蛋白,氯化钠、 C18和PSA净化,以XBridge peptide BEH C18(100 mm×2.1 mm,3.5μm)色谱柱分离,分别在正负离子模式下检测。结果显示,21种兽药在各自浓度区间内呈良好的线性关系(r>0.99),检出限为0.3~0.7μg/kg,定量限为1.0~2.5μg/kg,平均加标回收率为61.8%~110.7%,相对标准偏差为0.7%~17%。该方法可实现饲料中多类兽药的同时快速检测。 展开更多
关键词 QUECHERS 超高效液相色谱-串联质谱 饲料 兽药
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高效液相色谱-串联质谱法测定饲料中硝呋索尔代谢物残留量
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作者 魏云计 殷耀 +5 位作者 李瑞阳 刘利利 朱臻怡 冯民 徐松 丁涛 《化学分析计量》 2026年第3期94-99,共6页
建立高效液相色谱-串联质谱法测定饲料中硝呋索尔代谢物残留量。样品通过三氯乙酸水解,经2-硝基苯甲醛衍生,以乙酸乙酯提取,采用Agilent Polaris 5 C_(18)-A色谱柱分离,以乙腈-5 mmol/L乙酸铵溶液作为流动相梯度洗脱,柱温为35℃,进样体... 建立高效液相色谱-串联质谱法测定饲料中硝呋索尔代谢物残留量。样品通过三氯乙酸水解,经2-硝基苯甲醛衍生,以乙酸乙酯提取,采用Agilent Polaris 5 C_(18)-A色谱柱分离,以乙腈-5 mmol/L乙酸铵溶液作为流动相梯度洗脱,柱温为35℃,进样体积为10μL。采用电喷雾负离子源多反应监测模式进行检测,以内标法定量。硝呋索尔代谢物的质量浓度在0.5~25.0µg/L范围内与色谱峰面积线性关系良好,相关系数为0.9990,方法检出限为0.2μg/kg,定量限为0.5μg/kg。在低、中、高3个添加水平下,样品平均回收率为76.5%~104%,测定结果的相对标准偏差为5.8%~9.9%(n=6)。该方法适用于饲料中硝呋索尔代谢物残留量的检测。 展开更多
关键词 高效液相色谱-串联质谱法 饲料 硝呋索尔代谢物
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Influence of the Feed Gas Composition on the Fischer-Tropsch Synthesis in Commercial Operations 被引量:4
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作者 Theo Lee 《Journal of Natural Gas Chemistry》 EI CAS CSCD 2007年第4期329-341,共13页
Key technical challenges relating to the Fischer-Tropsch (F-T) synthesis applied in the commercialization of coal/gas-to-liquids (CTL/GTL) technologies have been reviewed. Based on the experiences accumulated from... Key technical challenges relating to the Fischer-Tropsch (F-T) synthesis applied in the commercialization of coal/gas-to-liquids (CTL/GTL) technologies have been reviewed. Based on the experiences accumulated from pilot plant, semi-work test and lab researches, the influences of the H2/CO ratio and the CO2 in the feed gas on the F-T process as well as on CTL/GTL complex in terms of product yields, energy efficiency and carbon utilization efficiency have been studied. Being contrary to the current design schemes for F-T process using the coal derived syngas and the iron-based cata lyst, it is suggested to feed the F-T synthesis unit with a syngas having a H2/CO ratio of 0.5 and then adjusting to 1.4 via the recycling process. As a result, the carbon efficiency of the whole plant could be reached to as high as 50%. For the issue of CO2 addition to the feed gas, it is proved that only a diluting role is played under the current commercial slurry phase F-T process. 展开更多
关键词 Fischer-Tropsch synthesis gas-to-liquids feed gas composition carbon efficiency
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Assessment of Poultry Feed Contamination Level by Aflatoxin B1: Quantification by Two Chromatographic Analysis Methods
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作者 Rokhaya Gueye Viviane Chatchueng Sandefo +5 位作者 Babacar Beye Elhadji Ousmane Faye Amadou Diop Serigne Omar Sarr Bara Ndiaye Yérim Mbagnick Diop 《Food and Nutrition Sciences》 CAS 2022年第11期950-961,共12页
Aflatoxin B1 is a mycotoxin that can contaminate a wide feedstuffs variety. Ingestion of contaminated feed by poultry can lead to impaired health and zootechnical performances but also a human diet safety problem rela... Aflatoxin B1 is a mycotoxin that can contaminate a wide feedstuffs variety. Ingestion of contaminated feed by poultry can lead to impaired health and zootechnical performances but also a human diet safety problem related to residues presence in animal origin products. Aflatoxin B1 contamination of poultry feed samples marketed in Dakar city and in peri-urban areas (Gorom, Sangalkam) was studied. A total of 15 samples were collected from Dakar city markets as well as from poultry farms in Gorom and Sangalkam areas. Aflatoxin B1 quantification was performed by high performance liquid chromatography and thin-layer chromatography. HPLC results showed that all samples were contaminated with levels ranging from 0.15 to 22 ppb, 0.099 to 2.05 ppb and 0.099 to 4.95 ppb respectively for Gorom, Sangalkam and Dakar. Only the finishing feed from Gorom had an aflatoxin B1 level above the maximum limit set by regulations. TLC is a suitable method for aflatoxins detection. However, it was associated with overestimation for aflatoxin B1 quantification. Results suggest that poultry feed represents a real source of human diet contamination. In addition, HPLC remains the most reliable quantification technique for quality control. 展开更多
关键词 Poultry feed AFLATOXIN High Performance liquid Chromatography Thin-Layer Chromatography DAKAR
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液体火箭推进剂交叉输送技术研究进展
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作者 魏祥庚 魏宇祺 +2 位作者 赵骁 王白岩 董译洹 《火箭推进》 北大核心 2025年第3期212-226,共15页
推进剂交叉输送技术能够有效提升飞行器的运载能力与动力冗余能力,是未来液体航天输运系统的重要发展方向。分析了交叉输送技术的优势,对其国内外应用现状开展了调研,总结了目前对交叉输送动静态特性方面的研究进展,并从应用需求的角度... 推进剂交叉输送技术能够有效提升飞行器的运载能力与动力冗余能力,是未来液体航天输运系统的重要发展方向。分析了交叉输送技术的优势,对其国内外应用现状开展了调研,总结了目前对交叉输送动静态特性方面的研究进展,并从应用需求的角度分析了交叉输送的方案设计与选择、与总体方案的匹配、动力冗余与控制以及关键部组件设计等重难点问题,对未来交叉输送技术可能的发展方向进行了展望,为未来大型液体运载火箭交叉输送技术的研究与应用提供参考。 展开更多
关键词 运载火箭 推进剂供应系统 推进剂交叉输送 液体火箭发动机
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饲料中盐酸氨丙啉、乙氧酰胺苯甲酯和磺胺喹噁啉的液相色谱-串联质谱测定方法研究
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作者 宋荣 李宏 +5 位作者 肖红年 严华 晁娟娟 魏书林 杨坤 雒亚璇 《中国饲料》 北大核心 2025年第23期108-114,共7页
本实验基于固相萃取和液相色谱-串联质谱仪(LC-MS/MS)技术,通过对目标化合物定性定量离子的筛选、色谱柱及流动相的选择、提取净化方法的优化、方法准确度和精密度等性能考察,旨在建立饲料中盐酸氨丙啉、乙氧酰胺苯甲酯和磺胺喹噁啉的... 本实验基于固相萃取和液相色谱-串联质谱仪(LC-MS/MS)技术,通过对目标化合物定性定量离子的筛选、色谱柱及流动相的选择、提取净化方法的优化、方法准确度和精密度等性能考察,旨在建立饲料中盐酸氨丙啉、乙氧酰胺苯甲酯和磺胺喹噁啉的液相色谱-串联质谱分析方法。试验结果表明:3种化合物的基质匹配定量曲线线性关系良好,相关系数(r)均大于0.999,检出限为0.02 mg/kg,定量限为0.05 mg/kg。饲料样品添加回收率为84%~112%,变异系数<10%。试验结果显示,该方法检出限低、灵敏度高、检测速度快,可以实现饲料中盐酸氨丙啉、乙氧酰胺苯甲酯和磺胺喹噁啉3种化合物的同步检测和准确定性定量分析。 展开更多
关键词 饲料 盐酸氨丙啉 乙氧酰胺苯甲酯 磺胺喹噁啉 液相色谱-串联质谱法
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构树叶液态发酵饲料对断奶仔猪生产性能及肠道菌群的影响 被引量:2
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作者 李嘉琪 朱绍伟 +2 位作者 夏尚远 夏尚飞 许西奎 《饲料工业》 北大核心 2025年第2期40-43,共4页
试验旨在研究添加构树叶的液态全价发酵饲料不同饲喂水平对断奶仔猪生产性能和肠道微生物菌群的影响,通过试验摸索出构树叶液态发酵饲料最佳饲喂水平。试验选取96头36日龄的杜长大三元健康断奶仔猪随机分为4个处理,每个处理4个重复,每... 试验旨在研究添加构树叶的液态全价发酵饲料不同饲喂水平对断奶仔猪生产性能和肠道微生物菌群的影响,通过试验摸索出构树叶液态发酵饲料最佳饲喂水平。试验选取96头36日龄的杜长大三元健康断奶仔猪随机分为4个处理,每个处理4个重复,每个重复6头猪,饲喂28 d,试验组为构树叶液态发酵饲料不同替代水平,即1/3替代固态全价日粮组(试验Ⅰ组)、2/3替代固态全价日粮(试验Ⅱ组)和全替代固态全价日粮(试验Ⅲ组),对照组为固态全价日粮,试验结束测定断奶仔猪生长性能及肠道大肠杆菌、乳酸菌的数量。试验结果表明,试验Ⅱ组的料重比、腹泻率显著低于对照组(P<0.05),十二指肠的大肠杆菌数量显著低于对照组(P<0.05),空肠段的乳酸菌数量显著高于对照组(P<0.05)。综上所述,构树叶液态全价发酵饲料替代2/3固态全价日粮,可提高断奶仔猪生长性能,改善断奶仔猪肠道菌群平衡。 展开更多
关键词 断奶仔猪 构树叶 液态 发酵饲料 肠道菌群
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液态发酵饲料对生长猪生长性能、肠道微生物区系及代谢产物的影响
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作者 王佰涛 雷高 +3 位作者 杨文玲 刁文涛 陈晓飞 刘德海 《饲料研究》 北大核心 2025年第22期28-32,共5页
试验旨在探究液态发酵饲料对生长猪生长性能、肠道微生物区系及代谢产物组成的影响。选取96头体重约为39 kg、健康状况良好的新丹系“长×大”二元生长猪,随机分为4组,每组3个重复,每个重复8头。A组采取干法饲喂基础饲粮,B组饲喂液... 试验旨在探究液态发酵饲料对生长猪生长性能、肠道微生物区系及代谢产物组成的影响。选取96头体重约为39 kg、健康状况良好的新丹系“长×大”二元生长猪,随机分为4组,每组3个重复,每个重复8头。A组采取干法饲喂基础饲粮,B组饲喂液态基础饲粮,C组饲喂液态发酵基础饲粮,D组饲喂液态发酵基础饲粮+0.005%复合酶制剂。预试期7 d,正式试验期28 d。结果显示:与A组相比,D组料重比显著降低(P<0.05);C组肠道微生物辛普森指数显著下降(P<0.05);C组和D组中特里斯孢菌属、布劳特氏菌属、罕见小球菌属的相对丰度明显增加,乳杆菌属的相对丰度明显降低。代谢组学分析表明,与A组相比,D组N-乙酰-L-谷氨酸-5-半醛、N-乙酰基-D-葡萄糖胺、四氢生物蝶呤等代谢产物显著上调(P<0.05),N-ω-乙酰基组胺、泛酸等代谢物显著下调(P<0.05);差异代谢物主要富集于精氨酸生物合成、核苷代谢、组胺代谢等与免疫及肠黏膜屏障密切相关的代谢通路。研究表明,液态发酵饲料可以有效降低生长猪的料重比,改善生长性能,调节肠道有益菌的相对丰度,改善肠道健康。 展开更多
关键词 生长猪 液态发酵饲料 生长性能 微生物区系 代谢组学
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猪肠道内源乳杆菌的筛选鉴定及其在液态发酵饲料中的应用 被引量:5
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作者 李耿辉 王雨君 +6 位作者 郑太龙 王松波 王丽娜 朱灿俊 吴睿帆 江青艳 束刚 《江西农业大学学报》 北大核心 2025年第1期152-166,共15页
【目的】筛选适用于猪液态发酵的肠道益生菌复合菌种,探究复合菌种制备的液态发酵饲料在育肥猪生产中的饲喂效果,为后续液态发酵猪饲料的应用提供研究基础。【方法】选取28日龄、体质量为8 kg左右的健康仔公猪肠道食糜,筛选出具备产酶... 【目的】筛选适用于猪液态发酵的肠道益生菌复合菌种,探究复合菌种制备的液态发酵饲料在育肥猪生产中的饲喂效果,为后续液态发酵猪饲料的应用提供研究基础。【方法】选取28日龄、体质量为8 kg左右的健康仔公猪肠道食糜,筛选出具备产酶和产酸特性的优势益生菌,对其进行分子生物学鉴定、安全性评价,并对其中一株益生菌进行全基因组测序与功能注释;根据优势益生菌制备的不同液态发酵饲料配方的发酵效果,采用一种最佳液态发酵方案用于饲养试验;选取120头120日龄、体质量约81 kg的丹系长白猪,随机分为对颗粒料照组(CON)、液态发酵料发酵组(FLF)两组,饲养28 d,统计育肥猪采食量、体增重、料重比等生长性能指标,并对粪便进行16S rRNA测序分析其微生物组成变化。【结果】(1)根据饲料底物降解酶活筛选出了活性最高的7株单克隆菌株:Hi04(谷糠乳杆菌,Lentilactobacillus farraginis)、Hi07(谷糠乳杆菌,Lentilactobacillus farraginis)、Hp06(鼠李糖乳杆菌,Lacticaseibacillus rhamnosus)、Hp09(鼠李糖乳杆菌,Lacticaseibacillus rhamnosus)、Hd02(希氏乳杆菌,Lactobacillus hilgardii)、Lm01(嘉义乳杆菌,Lacticaseibacillus chiayiensis)和Tc04(鼠李糖乳杆菌,Lacticaseibacillus rhamnosus),7株单克隆菌对抗生素耐药性均低于商业植物乳杆菌,其中,1株嘉义乳杆菌Lm01基因组中编码的碳水化合物代谢相关的功能基因丰度非常高,表明其具有非常强的代谢潜力,并其基因组中不含毒力基因;(2)以7株复合益生菌制备的液态发酵饲料,在添加10%起始菌种为1×107 CFU/mL发酵料二次发酵4 h后,饲料pH4.2,乳酸产量201.14 mmol/L,且跟商业乳杆菌相比,对饲料蛋白的降解能力更强,降解速度更快(P<0.05);(3)饲养试验结果表明:与对照组相比,液态发酵饲料组显著提高了育肥猪平均日增重,降低了耗料增重比(P<0.05);(4)16S rRNA测序结果发现:液态发酵饲料组显著提高了微生物群落的多样性(P<0.05),但降低了厚壁菌门和乳杆菌属的相对丰度(P<0.05)。【结论】基于产酸特征和产酶特性筛选出7株生物安全性好的猪肠道优势乳杆菌,该复合菌液态发酵饲料乳酸产量高且对饲料蛋白降解能力强,饲喂该复合益生菌液态发酵饲料可能通过调节肠道菌群,从而降低育肥猪的料重比。 展开更多
关键词 益生菌 液态发酵饲料 乳杆菌 育肥猪 生长性能
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应用液相色谱-串联质谱法检测饲料中5类41种抗生素的技术研究
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作者 王彬 《饲料工业》 北大核心 2025年第19期113-118,共6页
研究旨在探究饲料中5类(磺胺类、喹诺酮类、大环内酯类、青霉素类、烷胺类)41种抗生素的联合检测技术。选取市售饲料样品,采用PRiME HLB固相萃取柱法进行前处理,所得样液用液相色谱-串联质谱法进行测定,并计算该方法的准确性与精密度,... 研究旨在探究饲料中5类(磺胺类、喹诺酮类、大环内酯类、青霉素类、烷胺类)41种抗生素的联合检测技术。选取市售饲料样品,采用PRiME HLB固相萃取柱法进行前处理,所得样液用液相色谱-串联质谱法进行测定,并计算该方法的准确性与精密度,继而优化色谱条件和质谱条件并确定41种抗生素与对照品溶液浓度的线性关系。结果显示:基于Prime HLB固相萃取柱的饲料样品前处理,采用液相色谱-串联质谱检测5类41种抗生素,方法回收率为70%~110%,相对偏差≤15%。结果说明该方法操作便捷、准确、稳定、可靠。 展开更多
关键词 饲料 Prime HLB柱 抗生素 液相色谱-串联质谱法 联检 定性定量同步检测
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全自动免疫磁珠净化-高效液相色谱法测定饲料中脱氧雪腐镰刀菌烯醇
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作者 莫凡 金银姬 +5 位作者 姚婷 王枢昊 赵新雪 王石 邹宇飞 肖志明 《中国饲料》 北大核心 2025年第3期123-128,共6页
本研究旨在建立利用全自动免疫磁珠净化结合高效液相色谱测定饲料中脱氧雪腐镰刀菌烯醇(呕吐毒素)的方法。饲料样品用水提取,经全自动免疫磁珠净化后,采用高效液相色谱仪测定,外标法定量。结果表明:在浓度为0.05~2.0μg/mL呕吐毒素的线... 本研究旨在建立利用全自动免疫磁珠净化结合高效液相色谱测定饲料中脱氧雪腐镰刀菌烯醇(呕吐毒素)的方法。饲料样品用水提取,经全自动免疫磁珠净化后,采用高效液相色谱仪测定,外标法定量。结果表明:在浓度为0.05~2.0μg/mL呕吐毒素的线性关系良好(R^(2)=0.9999),方法的检出限为0.03 mg/kg,定量限为0.1 mg/kg;呕吐毒素在3个添加浓度(0.1、0.2、1.0 mg/kg)水平下的平均回收率为85.7%~102.5%,相对标准偏差为2.2%~9.6%。与传统固相萃取柱、免疫亲和柱净化相比,免疫磁珠净化集提取、分离、净化、浓缩于一体,可实现完全自动化处理,显著提高工作效率,避免因人为操作导致的结果偏差,适用于大批量饲料样本中呕吐毒素的测定。 展开更多
关键词 免疫磁珠净化 高效液相色谱 饲料 脱氧雪腐镰刀菌烯醇 呕吐毒素
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