Intake of nutraceuticals containing milk fat globule membranes(MFGMs)and extracellular vesicles(EVs)are purported to ameliorate age-related cognitive decline,which varies from mild cognitive impairment to dementia and...Intake of nutraceuticals containing milk fat globule membranes(MFGMs)and extracellular vesicles(EVs)are purported to ameliorate age-related cognitive decline,which varies from mild cognitive impairment to dementia and is a growing burden for healthcare systems worldwide.This study explored whether intake of an MFGM/EV-based ingredient,rich in polar milk lipids,mitigates cognitive decline in aged rats and modulates their brain lipidome.To do so,we carried out a 25-week long intervention study where 88-week-old rats were supplemented with the MFGM/EV-rich ingredient or a control milk lipid-deficient ingredient.At the end of the study,the rats were subjected to the Morris water maze test to assess their cognitive performance and biopsies of hippocampus,cerebral cortex and cerebellum were analyzed by in-depth MSALL lipidomics.For comparative purposes,we also included young 8-week-old rats in the study.Altogether,we found that the MFGM/EV-rich ingredient did not ameliorate age-related cognitive decline and mitigated only to a minor extent the age-driven remodeling of the brain lipidome.Furthermore,we provide the community with a comprehensive lipidomic resource detailing the lipid landscape and metabolic changes that occur in the brain as rats age.This resource can serve as a valuable reference for designing future nutritional therapies to rejuvenate the brain lipidome and improve cognitive function.展开更多
基金funded by(Danish Ministry of Food,Agriculture and Fisheries,34009-18-1456)the VILLUM Center for Bioanalytical Sciences(VKR023179).
文摘Intake of nutraceuticals containing milk fat globule membranes(MFGMs)and extracellular vesicles(EVs)are purported to ameliorate age-related cognitive decline,which varies from mild cognitive impairment to dementia and is a growing burden for healthcare systems worldwide.This study explored whether intake of an MFGM/EV-based ingredient,rich in polar milk lipids,mitigates cognitive decline in aged rats and modulates their brain lipidome.To do so,we carried out a 25-week long intervention study where 88-week-old rats were supplemented with the MFGM/EV-rich ingredient or a control milk lipid-deficient ingredient.At the end of the study,the rats were subjected to the Morris water maze test to assess their cognitive performance and biopsies of hippocampus,cerebral cortex and cerebellum were analyzed by in-depth MSALL lipidomics.For comparative purposes,we also included young 8-week-old rats in the study.Altogether,we found that the MFGM/EV-rich ingredient did not ameliorate age-related cognitive decline and mitigated only to a minor extent the age-driven remodeling of the brain lipidome.Furthermore,we provide the community with a comprehensive lipidomic resource detailing the lipid landscape and metabolic changes that occur in the brain as rats age.This resource can serve as a valuable reference for designing future nutritional therapies to rejuvenate the brain lipidome and improve cognitive function.
