Objective:To evaluate the influence of–250G>A(rs2070895)polymorphism in hepatic lipase gene(LIPC)promoter on plasma lipid parameters of ischemic stroke patients.Methods:A total of 100 stroke patients and 100 contr...Objective:To evaluate the influence of–250G>A(rs2070895)polymorphism in hepatic lipase gene(LIPC)promoter on plasma lipid parameters of ischemic stroke patients.Methods:A total of 100 stroke patients and 100 control subjects matched for sex(59 men and 41 women)and age were selected.Hepatic lipase activity and lipid profiles were measured while lipoprotein ratios were calculated.Genotyping of the–250G>A promoter polymorphism of the LIPC was performed by the polymerase chain reaction and restriction fragment length polymorphism method combined with 2%gel electrophoresis and then confirmed by direct sequencing.The LIPC promoter gene sequencing data were compared with refseqNG011465.1 LIPC from GenBank.Results:The frequencies of GG,GA and AA genotypes of LIPC rs2070895 polymorphism were 39%,45%and 16%for the control,10%,37%and 53%for the stroke subjects(P<0.0001),respectively.The frequencies of G and A alleles were 61.5%and 38.5%for the control,and 28.5%and 71.5%for the stroke subjects(P<0.0001).Our study shows that the mutant allele of the LIPC promoter was associated with dyslipidemia,lower hepatic lipase activity,and this variation contributed to the increased defective plasma high-density lipoprotein-cholesterol(HDL-C),HDL2-C and HDL3-C concentration for both subjects.The control subjects had 6 single nucleotide polymorphism and 6 amino acid substitutions while the stroke subjects had 32 single nucleotide polymorphism and 20 amino acid substitutions.Conclusions:LIPC–250G>A polymorphism can influence plasma lipid profiles and lipoprotein ratios in patients with ischemic stroke.展开更多
Background and Objectives:We aimed to investigate the association of triglyceride–glucose(TyG)index with hypertension and compare the discriminative power of the TyG index,lipid,glycemic parameters for hypertension u...Background and Objectives:We aimed to investigate the association of triglyceride–glucose(TyG)index with hypertension and compare the discriminative power of the TyG index,lipid,glycemic parameters for hypertension using the China Health Examination Collaborative study(CHEC Study).Methods and Study Design:Data were collected at Ningbo Mingzhou Hospital and Beijing physical examination center from the CHEC Study during 2014 and 2021.Participants with≥2 medical check-up times were included.The TyG index is the logarithmized product of fasting triglyceride and glucose.Generalised estimation equation(GEE)model was used to evaluate the association between the TyG index,lipid parameters,glycemic parameters and hypertension.Receiver operating characteristic(ROC)analysis was performed to explore the predictive ability of TyG index on hypertension at different years of medical check-up.Results:112,902 participants with an average age of 42.8 years were recruited in the study,36,839 participants developed hypertension over the 8-year period.GEE model analysis showed that the ORs with 95%CI of hypertension were 3.35(3.15-3.57),1.86(1.76-1.95),1.67(1.58-1.78),1.45(1.33-1.58),1.24(1.19-1.29),0.92(0.86-0.99),and 1.90(1.83-1.97)in the highest versus lowest quintiles of TyG index,TG/HDL-C ratio,TG,TC,LDL-C,HDL-C and FPG in model 2.The area under the ROC curve of the overall years of medical check-up was significantly higher than a particular year in predicting hypertension(AUC:0.883,p<0.05).Conclusions:TyG index is associated with hypertension and shows the superior discriminative ability for hypertension compared with lipid and glycemic parameters.展开更多
文摘Objective:To evaluate the influence of–250G>A(rs2070895)polymorphism in hepatic lipase gene(LIPC)promoter on plasma lipid parameters of ischemic stroke patients.Methods:A total of 100 stroke patients and 100 control subjects matched for sex(59 men and 41 women)and age were selected.Hepatic lipase activity and lipid profiles were measured while lipoprotein ratios were calculated.Genotyping of the–250G>A promoter polymorphism of the LIPC was performed by the polymerase chain reaction and restriction fragment length polymorphism method combined with 2%gel electrophoresis and then confirmed by direct sequencing.The LIPC promoter gene sequencing data were compared with refseqNG011465.1 LIPC from GenBank.Results:The frequencies of GG,GA and AA genotypes of LIPC rs2070895 polymorphism were 39%,45%and 16%for the control,10%,37%and 53%for the stroke subjects(P<0.0001),respectively.The frequencies of G and A alleles were 61.5%and 38.5%for the control,and 28.5%and 71.5%for the stroke subjects(P<0.0001).Our study shows that the mutant allele of the LIPC promoter was associated with dyslipidemia,lower hepatic lipase activity,and this variation contributed to the increased defective plasma high-density lipoprotein-cholesterol(HDL-C),HDL2-C and HDL3-C concentration for both subjects.The control subjects had 6 single nucleotide polymorphism and 6 amino acid substitutions while the stroke subjects had 32 single nucleotide polymorphism and 20 amino acid substitutions.Conclusions:LIPC–250G>A polymorphism can influence plasma lipid profiles and lipoprotein ratios in patients with ischemic stroke.
文摘Background and Objectives:We aimed to investigate the association of triglyceride–glucose(TyG)index with hypertension and compare the discriminative power of the TyG index,lipid,glycemic parameters for hypertension using the China Health Examination Collaborative study(CHEC Study).Methods and Study Design:Data were collected at Ningbo Mingzhou Hospital and Beijing physical examination center from the CHEC Study during 2014 and 2021.Participants with≥2 medical check-up times were included.The TyG index is the logarithmized product of fasting triglyceride and glucose.Generalised estimation equation(GEE)model was used to evaluate the association between the TyG index,lipid parameters,glycemic parameters and hypertension.Receiver operating characteristic(ROC)analysis was performed to explore the predictive ability of TyG index on hypertension at different years of medical check-up.Results:112,902 participants with an average age of 42.8 years were recruited in the study,36,839 participants developed hypertension over the 8-year period.GEE model analysis showed that the ORs with 95%CI of hypertension were 3.35(3.15-3.57),1.86(1.76-1.95),1.67(1.58-1.78),1.45(1.33-1.58),1.24(1.19-1.29),0.92(0.86-0.99),and 1.90(1.83-1.97)in the highest versus lowest quintiles of TyG index,TG/HDL-C ratio,TG,TC,LDL-C,HDL-C and FPG in model 2.The area under the ROC curve of the overall years of medical check-up was significantly higher than a particular year in predicting hypertension(AUC:0.883,p<0.05).Conclusions:TyG index is associated with hypertension and shows the superior discriminative ability for hypertension compared with lipid and glycemic parameters.
