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Deep Learning-Based Toolkit Inspection:Object Detection and Segmentation in Assembly Lines
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作者 Arvind Mukundan Riya Karmakar +1 位作者 Devansh Gupta Hsiang-Chen Wang 《Computers, Materials & Continua》 2026年第1期1255-1277,共23页
Modern manufacturing processes have become more reliant on automation because of the accelerated transition from Industry 3.0 to Industry 4.0.Manual inspection of products on assembly lines remains inefficient,prone t... Modern manufacturing processes have become more reliant on automation because of the accelerated transition from Industry 3.0 to Industry 4.0.Manual inspection of products on assembly lines remains inefficient,prone to errors and lacks consistency,emphasizing the need for a reliable and automated inspection system.Leveraging both object detection and image segmentation approaches,this research proposes a vision-based solution for the detection of various kinds of tools in the toolkit using deep learning(DL)models.Two Intel RealSense D455f depth cameras were arranged in a top down configuration to capture both RGB and depth images of the toolkits.After applying multiple constraints and enhancing them through preprocessing and augmentation,a dataset consisting of 3300 annotated RGB-D photos was generated.Several DL models were selected through a comprehensive assessment of mean Average Precision(mAP),precision-recall equilibrium,inference latency(target≥30 FPS),and computational burden,resulting in a preference for YOLO and Region-based Convolutional Neural Networks(R-CNN)variants over ViT-based models due to the latter’s increased latency and resource requirements.YOLOV5,YOLOV8,YOLOV11,Faster R-CNN,and Mask R-CNN were trained on the annotated dataset and evaluated using key performance metrics(Recall,Accuracy,F1-score,and Precision).YOLOV11 demonstrated balanced excellence with 93.0%precision,89.9%recall,and a 90.6%F1-score in object detection,as well as 96.9%precision,95.3%recall,and a 96.5%F1-score in instance segmentation with an average inference time of 25 ms per frame(≈40 FPS),demonstrating real-time performance.Leveraging these results,a YOLOV11-based windows application was successfully deployed in a real-time assembly line environment,where it accurately processed live video streams to detect and segment tools within toolkits,demonstrating its practical effectiveness in industrial automation.The application is capable of precisely measuring socket dimensions by utilising edge detection techniques on YOLOv11 segmentation masks,in addition to detection and segmentation.This makes it possible to do specification-level quality control right on the assembly line,which improves the ability to examine things in real time.The implementation is a big step forward for intelligent manufacturing in the Industry 4.0 paradigm.It provides a scalable,efficient,and accurate way to do automated inspection and dimensional verification activities. 展开更多
关键词 Tool detection image segmentation object detection assembly line automation Industry 4.0 Intel RealSense deep learning toolkit verification RGB-D imaging quality assurance
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In vitro Plant Regeneration of Pepper Cytoplasmic Male Sterility (CMS) Lines via Cotyledon Culture 被引量:14
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作者 邓明华 文锦芬 邹学校 《Agricultural Science & Technology》 CAS 2009年第1期39-42,共4页
An in vitro shoot regeneration procedure was developed in pepper ( Capsicum annuum L. ) cytoplasmic male sterility (CMS) lines 9704A and 8214A using cotyledon as explant. The callus and bud cluster derived from co... An in vitro shoot regeneration procedure was developed in pepper ( Capsicum annuum L. ) cytoplasmic male sterility (CMS) lines 9704A and 8214A using cotyledon as explant. The callus and bud cluster derived from cotyledon tissue explants were proliferated on Murashige and Skoog (MS) medium supplemented with different combinations of 6-benzladenine (6-BA), indole-3-acetic acid (IAA), gibberellic acid (GA3) and silver nitrate (AgNO3). From the formula of MS appended with 5.0 mg/L 6-BA, 1.0 mg/L IAA and 5.0 mg/L AgNO3, for the explants callus and bud cluster, the maximum differentiation rates ( respectively 100.0% and 58.3% ) and average number of adventitious bud from each explant (respectively 18.8 and 13.2) were obtained. The optimum medium combination for the elongation of adventitious bud was determined to be: MS + 3.0 mg/L 6-BA + 1.0 mg/L IAA + 5.0 mg/L AgNO3 + 2.0 mg/L GA3, from which the elongation rates of buds from callus and bud cluster were both 100%, and the average number of per explant adventitious bud number reached 6.3 and 5.8, respectively. And all the elongated shoots were successfully rooted on half-strength MS medium supplemented with 0.3-0.5 mg/L IAA. 展开更多
关键词 Capsicum annuum L. CMS line Cotyledon culture Plant regeneration
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Characterization of Fertility Alteration in 8 Environment-sensitive Genic Male-sterile Lines of indica Rice 被引量:21
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作者 牟同敏 曹兵 +3 位作者 黄宗洪 杨国才 林尤珍 向关伦 《Acta Botanica Sinica》 CSCD 2001年第3期238-242,共5页
Eight indica ( Oryza sativa L.) environment-sensitive genic male-sterile (EGMS) lines, 2-2S, K1405S, F131S, 2136S, Pei-Ai 64S, 1290S, GD-IS and N17S, were sequentially seeded with 10-15 d interval at three sites, Wuha... Eight indica ( Oryza sativa L.) environment-sensitive genic male-sterile (EGMS) lines, 2-2S, K1405S, F131S, 2136S, Pei-Ai 64S, 1290S, GD-IS and N17S, were sequentially seeded with 10-15 d interval at three sites, Wuhan in 1997, Guiyang in 1997 and Sanya in 1997 and 1998, China. The results of investigations on self-sterilities showed that all of eight EGMS lines had stable sterile periods of longer than 30 d at Wuhan. They can be used for seed production of two-line hybrid rice, but can not reproduce themselves. Their stable sterile periods were shorter than 30 d at Guiyang, they can reproduce themselves and can not be used for hybrid seed production. In Sanya, their stable sterile periods were longer than 150 d, all of eight lines can be used for seed production in summer and autumn and reproduce themselves in winter. The fertility of all eight lines were sensitive to temperature. The sensitive stages, sensitive duration and critical point of temperatures (CPT) of fertility alteration in various lines were different. The sensitive stages of 2-2S and K1405S were from 18 d to 9 d before heading, the sensitive durations were 7-10 d and the CPTs were 23.7-24.5 degreesC. The sensitive stage, sensitive duration and CPT of F131S were from 17 to 5 d before heading, 13 d and 24.3-24.7 degreesC, respectively. The sensitive stage, sensitive duration and CPT of 2136S were from 18 to 12 d before heading, 7 d and 24.6-25.1 degreesC:, respectively. The sensitive stages, sensitive durations of Pei-Ai 64S, 1290S, N17S and GD-1S were from 24 to 13 d before heading and 10-13 d. And their CPTs were 24.6-25.1 degreesC, 25.5-26.2 degreesC, 25.4-26.1 degreesC,, and 24.1-24.7 degreesC, respectively. 展开更多
关键词 RICE environment-sensitive genic male-sterile lines fertility expression sensitive stage and duration critical point temperature of fertility alteration
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Correlation between Stigma Characteristics and Outcrossing Rate of Five Photo-thermo Sensitive Genic Male Sterile(PTGMS) Rice Lines 被引量:2
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作者 舒志芬 陈勇 +3 位作者 刘钊 袁露 熊朝 张海清 《Agricultural Science & Technology》 CAS 2016年第3期573-576,583,共5页
[Objective] This study aimed to investigate the correlation between stigma characteristics and outcrossing rate of photo-thermo sensitive genic male sterile(PTGMS) rice lines. [Method] The stigma exsertion rate, sti... [Objective] This study aimed to investigate the correlation between stigma characteristics and outcrossing rate of photo-thermo sensitive genic male sterile(PTGMS) rice lines. [Method] The stigma exsertion rate, stigma size and stigma vitality of Chuang 5S, Yun 9S, Peiai 64 S, P88 S and Y58 S were analyzed. Using R608 as a male parent, outcrossing rate of these five PTGMS lines was determined. [Result]There were differences in stigma exsertion rate, stigma size and stigma vitality among five PTGMS lines. P88 S exhibited the best stigma characteristics and relatively strong stigma vitality, with the stigma exsertion rate of 95.94%, stigma vitality coefficient of 2.71, outcrossing rate of 84.02% in the field. Peiai 64 s had the minimum stigma length(2.41 mm) and stigma width(1.10 mm) and the weakest stigma vitality, with the stigma exsertion rate of 93.04%, outcrossing rate of 65.47%. Yun 9S,Y58 S and Chuang 5S displayed medium stigma characteristics, among which Yun9 S exhibited higher outcrossing rate than other two PTGMS lines. Correlation analysis showed that stigma exsertion rate, stigma length, stigma width, stigma area and stigma volume were significantly correlated to outcrossing rate(P〈0.05), with correlation coefficients of 0.897, 0.934, 0.934, 0.939 and 0.937, respectively. However,there was no significant correlation between stigma vitality coefficient and outcrossing rate. [Conclusion] Based on stigma characteristics of five PTGMS lines, auxiliary pollination measures should be adopted reasonably to save production costs and improve hybrid rice seed yield. 展开更多
关键词 RICE PTGMS lines Stigma characteristics Outcrossing rate
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Recurrent Selection for Improving Quality Characters of Male Sterile Restorer Lines of Brassica napus
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作者 沈奇 秦信蓉 +2 位作者 向阳 赵跃 杜才富 《Agricultural Science & Technology》 CAS 2013年第5期763-766,共4页
[Objective] This study aimed to improve the quality characters of the male sterile restorer lines of Brassica napus by recurrent selection. [Method] The dominant male sterile alleles were used as outcross medium to be... [Objective] This study aimed to improve the quality characters of the male sterile restorer lines of Brassica napus by recurrent selection. [Method] The dominant male sterile alleles were used as outcross medium to be transformed with the favourable genes from thirteen materials, and finally excellent restorer materials were bred by recurrent selection. After that, the genetic diversity, genetic gains, fatty acid chain length and unsaturarion ratios of the improved populations were analyzed. [Result] The genetic diversity and genetic gains were obviously improved by the recurrent selection. Nine materials whose oil content exceeding 50% and 14 materials whose protein content exceeding 30% were acquired, and crossed with sterile lines. The comparative experiment showed that there were 11 combinations whose oil content was obviously enhanced compared with the control. [Conclusion] Recurrent selection is an effective way to improve the quality characters of the objective pop- ulations and breed favourable male sterile restorer lines of B. napus. 展开更多
关键词 Brassica napus Recurrent selection Male sterile lines Population improvement
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Flowering Characteristics of Japonica Rice Male Sterile Lines Adaptive to High Temperature and Summer Drought
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作者 肖人鹏 李经勇 +3 位作者 姚雄 张现伟 文明 唐永群 《Agricultural Science & Technology》 CAS 2015年第8期1621-1627,1647,共8页
[Objective] This study was conducted to screen japonica rice male sterile lines with good flowering characteristics under high temperature and summer drought. [Method] The flowering habits of 23 japonica male sterile ... [Objective] This study was conducted to screen japonica rice male sterile lines with good flowering characteristics under high temperature and summer drought. [Method] The flowering habits of 23 japonica male sterile lines, such as flowering peak, single-panicle flowering duration, single-plant flowering duration, en- closed glume rate, glume-opening angle, the distance between glumes, stigma exer- tion rate and sigma vitality, were compared with II-32A as a control under above weather condition. [Result] The flowering characteristics had significant difference a- mong the tested rice lines. Compared with the control, three japonica rice male ster- ile lines 35478A, 35489A and 35502A had better flowering traits, such as earlier flowering peak, longer single-panicle flowering duration, longer single-plant flowering duration, lower rate of enclosed glumes, larger glume angle and larger distance be- tween glumes. 35478A performed its flowering peak from 10:00-11:00 am, 1.5 h earlier than the control; 35489A exhibited higher stigma exertion rate, up to 76.23%; and 35502A had higher stigma vitality, up to 84.68%, which kept longer time than that of the CK. [Conclusion] The results will provide high-quality resources for the breeding of new japonica rice varieties with high-yield and high-temperature toler- ance. 展开更多
关键词 Japonica rice Male Sterile line High temperature Summer drought Flowering characteristic
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Characterization of Reverse Thermo-sensitive Genic Male Sterile Lines in Cold Regions of China
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作者 高扬 谭炎宁 +2 位作者 孟昭河 段美娟 徐秋生 《Agricultural Science & Technology》 CAS 2017年第7期1183-1186,1201,共5页
Yannong S, a reverse thermo-sensitive genic male sterile (TGMS) line, is sterile at high temperature and fertile at low temperature. In the present study, the fertility of Yannong S and the japonica reverse TGMS lin... Yannong S, a reverse thermo-sensitive genic male sterile (TGMS) line, is sterile at high temperature and fertile at low temperature. In the present study, the fertility of Yannong S and the japonica reverse TGMS lines derived from it was in- vestigated in Harbin, Heilongjiang from 2013 to 2015. The results showed that Yan- nong S was completely male sterile in July and August (the hottest months of the year in Harbin), and the seed setting rate of bagged panicles was 0. We also in- ferred that the critical temperature for inducing the sterility of Yannong S was higher than 28 ℃. Most of the japonica reverse TGMS lines derived from Yannong S were completely aborted in July and August, with a seed setting rate of 0. It can be con- cluded that the reverse TGMS lines derived from Yannong S are genetically stable lines that have a long period of sterility in cold regions, so they may be the ideal genetic materials for the development and in-situ seed production of japonica hybrid rice. 展开更多
关键词 Japonica hybrid rice Reverse thermo-sensitive genic male sterile lines Cold regions Yannong S FERTILITY
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Effect of High Temperature on Outcrossing Characteristics at the Fertility Sensitive Period of Photo-thermo Sensitive Genic Male Sterile (PTGMS) Rice Lines
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作者 王明 刘烨 +6 位作者 张海清 刘爱民 肖层林 唐荣 杨永标 蒋凌云 李雪雪 《Agricultural Science & Technology》 CAS 2017年第11期2019-2025,共7页
Four sterile lines (Peiai64S, Y58S, Guangzhan 63-2S and H638S) and the restoring line R527 were used as materials. Five temperature gradients (24, 27, 30, 33 and 36 ℃ in artificial climate chamber) and the natura... Four sterile lines (Peiai64S, Y58S, Guangzhan 63-2S and H638S) and the restoring line R527 were used as materials. Five temperature gradients (24, 27, 30, 33 and 36 ℃ in artificial climate chamber) and the natural temperature (as control) were treated to the four sterile lines for 6 d in the fertility sensitive period of heading to flowering stage, respectively, to study the effects of temperature on physiological biochemical characteristics of young panicles and outcrossing characteristics. The results showed that the percentages of exerted stigma of Peiai 64S and Y58S were the highest at 27 ℃, which were 86.81% and 86.06%, respectively, while the percentages of exerted stigma of Guangzhan 63-2S and H638S were the highest at 24 ℃, which were 76.24% and 81.76%, respectively; the stigma viability of Peiai 64S, Y58S, Guangzhan 63-2S and H638S were the best at 24 ℃, which were 1.96, 2.12, 1.74 and 1.94, respectively; the outcrossing rates of Peiai 64S, Guangzhan 63-2S and H638S were the highest at 24 ℃, which were 58.87%, 54.22% and 50.50%, respectively, while the outcrossing rate of Y58S was the highest at 27 ℃, which was 58.96%; and the contents of peroxidase (POD) of the four sterile lines increased significantly at 33 ℃ compared with the control, while the contents of malondialdehyde (MDA) and proline increased significantly at 36 ℃ compared with the control. There were differences in temperature sensitivity between the male sterile lines, and the 24 ℃ treatment during the sensitive period was the best for the fertility sensitive period of Peiai 64S, while 27 ℃ was the best temperature for Y58S, Gangzhan 63-2S and H638S. 展开更多
关键词 RICE Photo-thermo sensitive male sterile line Fertility sensitive period Temperature Outcrossing characteristics Physiological and biochemical characteristics
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Comparisons on Genetic Diversity among the Isonuclear-Alloplasmic Male Sterile Lines and Their Maintainer Lines in Rice 被引量:1
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作者 LI Jin-quan CAI Shan-xin FENG Jiu-huan LI Wei CHENG Gui-ping 《Rice science》 SCIE 2007年第2期94-100,共7页
Four sets of rice isonuclear-alloplasmic lines including 16 male sterile lines and their maintainer lines were analyzed by using 91 pairs of SSR primers to study the genetic diversity of nuclear genome and their relat... Four sets of rice isonuclear-alloplasmic lines including 16 male sterile lines and their maintainer lines were analyzed by using 91 pairs of SSR primers to study the genetic diversity of nuclear genome and their relative relationships. A total of 169 alleles were detected in the 16 lines, with a frequency of polymorphic loci of 53.85% and an average number of alleles per locus of 1.8, and the average gene diversity was 0.228. Four sets of the isonuclear-alloplasmic male sterile lines shared 146 identical alleles, corresponding to 86.39% of the total alleles; meanwhile, there are 23 different alleles among the tested materials, being 13.61% of the total alleles. On average, 78.70% identical alleles and 21.30% different alleles of the total alleles were detected between the isonuclear-alloplasmic male sterile lines and their maintainer lines. There were 53.85% identical alleles and 46.15% different alleles of the total alleles among the homozygous allonucleus male sterile lines. The fingerprints were established for some male sterile lines and maintainer lines. All the materials tested were divided into three groups at the 0.2 genetic distance based on the cluster analysis. Eight lines of Huanong A and Huayu A (including Huanong B and Huayu B) were in the first group, four lines of Kezhen A (including Kezhen B) in the second group, and four lines of Zhenshan 97A (including Zhenshan 97B) in the third group. For the isonuclear-alloplasmic male sterile lines, the similarity coefficient between Y (Yegong) type and WA (wild abortive) type or between CW (Raoping wild rice) and WA type reached 87-98%. 展开更多
关键词 hybrid rice isonuclear-alloplasmic male sterile line maintainer line genetic diversity
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Differences in Nuclear DNA Between Male-Sterile and Male-Fertile Lines of Sorghum bicolor 被引量:1
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作者 FANChang-fa SUNChun-yun +2 位作者 GUOXiao-cai NIUTian-tang ZHANGFu-yao 《Agricultural Sciences in China》 CAS CSCD 2003年第7期736-741,共6页
Cytoplasmic male sterility(cms)is determined by nuclear-cytoplasmic interactions. Up to now, most studies are focused on the comparison of cytoplasmic DNAs of male-sterile lines and male-fertile lines, and analysis of... Cytoplasmic male sterility(cms)is determined by nuclear-cytoplasmic interactions. Up to now, most studies are focused on the comparison of cytoplasmic DNAs of male-sterile lines and male-fertile lines, and analysis of nuclear DNA has not been documented yet. In order to find out the possible difference in nuclear genome of male-sterile line A1 Tx623 and corresponding male-fertile line Tx623 of sorghum, random amplified polymorphic DNA(RAPD)approach was used to analyze their cytoplasmic and nuclear genomes. Total DNAs of them were amplified at first to screen primers, which were able to generate reproducible bands specific to male-sterile line or male-fertile line. Then the selected primers were used to amplify their mitochon-drial DNA(mtDNA)and chloroplast DNA(cpDNA). The origins of all the polymorphic fragments were analyzed. After ruling out those amplified from cytoplasmic DNA, seventeen polymorphic fragments were determined to be amplified from nuclear DNA. These fragments originated from nuclear DNA indicate that differences in sequence exist between the nuclear DNA of male-sterile line and male-fertile line of sorghum, which do not agree with the traditional standpoint that they have identical nucleus. 展开更多
关键词 Cytoplasmic male sterility Sorghum bicolor RAPD Male-sterile line Male-fertile line Difference in nuclear DNA
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Development and Application of SCAR Markers for Discriminating Cytoplasmic Male Sterile Lines from Their Cognate Maintainer Lines in Indica Rice 被引量:1
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作者 LU Chao LIU Jian +4 位作者 JIANG Jian-hua Caleb Manamik BRERIA TAN He-lin Masahiko ICHII HONG De-lin 《Rice science》 SCIE 2013年第3期191-199,共9页
The DNAfragments about 1 600 bp were amplified using random amplified polymorphism DNA (RAPD) primer OPAl2 with the templates of mitochondrial DNA of Zhenshan 97A and Zhenshan 97B, and were sequenced. The nucleotide... The DNAfragments about 1 600 bp were amplified using random amplified polymorphism DNA (RAPD) primer OPAl2 with the templates of mitochondrial DNA of Zhenshan 97A and Zhenshan 97B, and were sequenced. The nucleotide sequences and lengths of the fragments from Zhenshan 97A and Zhenshan 97B showed no difference. The precise length of the fragment was 1 588 bp. Sequence characterized amplification region (SCAR) primers were then developed to discriminate the cytoplasmic male sterile (CMS) lines and their maintainer lines. A specific 1 588 bp fragment could be amplified with SCAR primers, CHI19F2/CHI19R2 and CHI20F3/CHI23R3, in the mitochondrial DNA of Zhenshan 97A, but not Zhenshan 97B. Furthermore, the specific fragment could be also amplified from the total DNA from green leaf tissues of Zhenshan 97A with SCAR primers, but not Zhenshan 97B. With the corresponding primers, the specific fragment could also be amplified from the total DNA of green leaves of other two CMS lines with wild abortive type cytoplasm (CMS-WA), namely Zhenpin A and Tianfeng A, but not in their maintainer lines. Moreover, using total DNA as template, each of the four pairs of SCAR primers could also be used to amplify the 1 588 bp fragment in CMS-ID (Indonesia paddy type) line 11-32A but not in 11-32B, and the specific fragment was amplified from the DNA of both F1 and F2 seedlings of Shanyou 63. The results of detecting the genetic purity of a man-made mixture of the seeds of Zhenshan 97A using CHI20F3/CHI23R3 were completely consistent with the phenotypes. Taken together, these results indicated that the specific 1 588 bp-fragment amplified by CHI20F3/CHI23R3 was the unique amplification products of CMS mitochondrial DNA, and could be used to distinguish CMS-WA and CMS-ID lines from their corresponding maintainer lines at the seedling stage. 展开更多
关键词 Oryza safiva cytoplasmic male sterile line maintainer line genetic purity sequence characterized amplification region marker
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New Temperature Sensitive Genic Male Sterile Lines with Better Outcrossing Ability for Production of Two-Line Hybrid Rice 被引量:1
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作者 S.J.ARASAKESARY S.MANONMANI +1 位作者 R.PUSHPAM S.ROBIN 《Rice science》 SCIE CSCD 2015年第1期49-52,共4页
An investigation was carried out with three newly developed temperature sensitive genic male sterile (TGMS) lines for their floral traits, seed production potential and outcrossing ability in ten cross combinations.... An investigation was carried out with three newly developed temperature sensitive genic male sterile (TGMS) lines for their floral traits, seed production potential and outcrossing ability in ten cross combinations. In the TGMS lines, fertile pollens had an average diameter of 0.89 mm while the sterile pollens was with 0.02 mm diameter.TS-29-150GY produced the biggest fertile pollens with 0.92 mm and other two lines produced relatively smaller pollens with 0.91 and 0.85 ram. Pollen fertility during the fertility reversion period was an average of 60.7%. TS-29-150GY had the maximum of 66.9% spikelet fertility whereas other two lines (TNAU18S and TNAU60S) had relatively lower spikelet fertility of 27.8% and 26.7%, respectively. Average of 17.00 g of seed yield was obtained in the TGMS lines during the fertility reversion period. TS-29- 150GY had the highest value of 21.20 g of seed yield while TNAU18S and TNAU60S produced 16.6 g and 13.2 g of seed yield, respectively. The low seed production ability of these three TGMS lines was attributed only to the environmental conditions prevailing during the period. All three TGMS lines had considerable outcrossing potential of 41.2%, 24.6% and 25.0%, respectively. The cross combinations viz. TNAU18S/IET21508 (36 g/plant), TNAU18S/IET21044 (13 g/plant), TNAU18S/IET21009 (26.5 g/plant), TNAU60S/CB-09-106 (26.2 g/plant), TNAU60S/IET21009 (14 g/plant) and TS29-150-GY/DRR 3306 (39.2 g/plant) showed perfect synchronization with acceptable hybrid seed yield, indicating suitability of TGMS system under Indian condition. Based on the outcrossing related traits viz. panicle exertion, angle of glume opening, stigma length and pollen size, TNAU18S was identified as the best, followed by TS-29-150GY. 展开更多
关键词 temperature sensitive genic male sterile line OUTCROSSING floral trait hybrid rice
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Correlation Analysis on Male Sterile Lines Characters in Rapeseed(Brassica napus L.)
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作者 黄泽素 Laosuwan Paisan +4 位作者 Machikowa Thitiporn 陈泽辉 代文东 唐容 李德珍 《Agricultural Science & Technology》 CAS 2010年第9期183-187,共5页
[Objective] This study was conducted to evaluate the associations of many characters of male sterile lines in Brassica napus L.[Method]10 recessive genetic male sterile(RGMS)lines were used as parents to cross in a ... [Objective] This study was conducted to evaluate the associations of many characters of male sterile lines in Brassica napus L.[Method]10 recessive genetic male sterile(RGMS)lines were used as parents to cross in a half diallel cross method to produce 45 single cross hybrids.These 45 crosses and their 10 parents were evaluated at Guiyang during 2007-2008.[Result]The positive correlations of seed yield with plant height,pods per plant,days to flowering and days to maturity were significant.Their respective correlation coefficients(r)were 0.644,0.583,0.281 and 0.341.Highly significant and negative correlation was observed between seeds per pod and seed size(-0.533).Direct path coefficients were observed for pods per plant(0.556 2),seeds per pod(0.407 4),1 000-seed weight(0.481 3),and plant height(0.401 7)to seed yield.[Conclusion]Pods per plant,seeds per pod,1 000-seed weight and plant height were positive correlated with seed yield and showed high direct contributions to yield.Other characters including branches per plant,days to flowering,days to maturity and oil content had low contributions to seed yield. 展开更多
关键词 Brassica napus L. Male sterile line Correlation analysis CHARACTERS
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Analysis of Introgressed Segments in Near-isogenic Lines for F_1 Pollen Sterility in Rice (Oryza sativa)
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作者 Akshay TALUKDAR 《Rice science》 SCIE 2003年第1期1-5,共5页
One hundred and fifty-eight microsatellite markers showing polymorphism among parents were used to survey the introgressed segments in the 50 near-isogenic lines of F_1 pollen sterility. Two hundred and sixty introgre... One hundred and fifty-eight microsatellite markers showing polymorphism among parents were used to survey the introgressed segments in the 50 near-isogenic lines of F_1 pollen sterility. Two hundred and sixty introgressed segments were detected in 50 near-isogenic lines, each carrying 5.2 introgressed segments on an average. Among the 260 segments, one hundred carrying F_1 pollen sterility loci concentrated on the region of F_1 pollen sterility genes, and the remaining one hundred and sixty without F_1 pollen sterility loci distributed randomly over 12 chromosomes. Both the average number and length of the introgressed segments decreased along with the increase of backcross generations. The number of introgressed segments was less than four and the length was less than 20 cM in the near-isogenic lines after backcrossing for four or more times. 展开更多
关键词 introgressed segment near-isogenie line pollen sterility RICE
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Analysis on Combining Ability for Characters of Male Sterile Lines in Rapeseed(Brassica napus L.)
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作者 Huang Ze-su Laosuwan Paisan +4 位作者 Machikowa Thitipom Chen Ze-hui Dai Wen-dong Tang Rong Li De-zhen 《Journal of Northeast Agricultural University(English Edition)》 CAS 2012年第3期28-37,共10页
The male sterile line is very important in the hybrid breeding program of rapeseed. This study was conducted to evaluate the combining ability of many characters of male sterile lines in Brassica napus L. Ten recessiv... The male sterile line is very important in the hybrid breeding program of rapeseed. This study was conducted to evaluate the combining ability of many characters of male sterile lines in Brassica napus L. Ten recessive genetic male sterile (RGMS) lines were used as parents to produce 45 single cross hybrids by using a half diallel cross method. These 45 crosses and their 10 parents were evaluated at Guiyang during 2007-2008. The results showed that both general combining ability (GCA) and specific combiing ability (SCA) effects were important for all characters, but additive gene effects were more predominant than non-additive gene effects. Qianyou 8AB and You 2894AB gave respective highly significant GCA effects of 230.94 and 127.65 kg-hm-2 for seed yield. Lines You 2894AB, QH303-4AB, You 157AB and You 2341AB gave highly significant GCA effects for oil content of 0.99, 1.62, 1.20 and 1.53%, respectively. The crosses among lines Qianyou 3AxQianyou 8B, Qianyou 8AxYou 2894B, You 2894AxQianyou 6B, Qianyou 8AxQH303-4B and Qianyou 8Ax Qianyou 6B gave high SCA effects of 616.29, 398.71,356.48, 394.24 and 303.79 kg hm-2 for seed yield, respectively. All these crosses also gave high seed yield indicating that these crosses could be used in the breeding program. 展开更多
关键词 Brassica napus L. male sterile line combining ability CHARACTER
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Genetic Mechanism of Dominant Earliness in Kefeng A,a New Rice Cytoplasmic Male Sterile Line
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作者 XIAO Yu-long Yu Chuan-yuan +3 位作者 LEI Jian-guo LI Ma-zhong JIANG Lin WAN Jian-min 《Rice science》 SCIE 2009年第4期267-273,共7页
Kefeng A is an early maturing indica cytoplasmic male sterile (CMS) line of rice. Combinations derived from Kefeng A and late maturing indica restorer lines showed dominant earliness to various extents. To understan... Kefeng A is an early maturing indica cytoplasmic male sterile (CMS) line of rice. Combinations derived from Kefeng A and late maturing indica restorer lines showed dominant earliness to various extents. To understand the genetic basis of dominant earliness, the genotype of photoperiod-sensitive genes in Kefeng A was analyzed using a complete set of heading time near isogenic lines (NILs) EGO to EG7, ER, LR, T65, T65E^b, T65E^bm, T65m, NIL(Hd1) and NIL(Hd4). Results indicated that Kefeng A contained two dominant photoperiod-sensitive alleles E1 and Se-1^U on E1 and Se-1 loci, respectively, and the genotype of photoperiod-sensitivity genes for heading time in Kefeng A was E1E1e2e2E3E3Se-1^USe-1UEf-1Ef-1. Based on the detected heading time genotype, in combination with the heading time of Kefeng A and the early maturing phenomenon in its derived F1 hybrids, it is speculated that Kefeng A might carry a dominant inhibitor gene Su-E7 for the dominant photoperiod- sensitive gene E1, and a recessive inhibitor gene i-Se-1 for another dominant photoperiod-sensitive gene Se-1. The reason why F1 hybrids from Kefeng A exhibited early maturing was hereby analyzed and the breeding value of dominant earliness related genes in Kefeng A was discussed. 展开更多
关键词 dominant earliness heading time GENOTYPE genetic mechanism cytoplasmic male sterile line RICE
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利用FAD2分子标记构建花生荚果大小RIL群体
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作者 胡畅丽 李鑫 +7 位作者 李晶晶 纪红昌 唐艳艳 邹晓霞 姜德锋 郭宝太 王晶珊 乔利仙 《分子植物育种》 北大核心 2025年第20期6751-6756,共6页
遗传群体是作物遗传图谱构建、新基因挖掘以及种质资源利用的基础。本研究为花生荚果大小基因定位构建重组近交系(recombinant inbred line,RIL)遗传群体,首先利用A hFAD2基因型为aaBB的大果花生品种‘鲁花11’和基因型为aabb的小果花... 遗传群体是作物遗传图谱构建、新基因挖掘以及种质资源利用的基础。本研究为花生荚果大小基因定位构建重组近交系(recombinant inbred line,RIL)遗传群体,首先利用A hFAD2基因型为aaBB的大果花生品种‘鲁花11’和基因型为aabb的小果花生品系BC54进行杂交获得F_(1),利用KASP标记检测F_(1)基因型筛选真杂种aaBb,然后单粒播种杂种F_(1),收获F_(2)株系。测量每个F_(2)株系荚果长度,选留荚果长度存在分离表现的株系。对于荚果长度无明显分离的株系,取F_(2)各单株叶片等量混合后提取DNA,利用KASP标记检测FAD2的基因型,保留出现FAD2b位点的杂合株系、淘汰基因型为aaBB的纯合株系。经二次筛选的真杂种后代通过连续自交选育出包含520个家系的RIL群体。本研究利用非目标性状FAD2分子标记进行F_(1)杂种鉴定,有效节约时间和成本,成功构建RIL群体,为花生荚果大小基因定位和新品种选育提供了定位群体及基因资源。 展开更多
关键词 花生 ril群体 FAD2标记 KASP 荚果大小
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GFRF R-CNN:Object Detection Algorithm for Transmission Lines
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作者 Xunguang Yan Wenrui Wang +3 位作者 Fanglin Lu Hongyong Fan Bo Wu Jianfeng Yu 《Computers, Materials & Continua》 SCIE EI 2025年第1期1439-1458,共20页
To maintain the reliability of power systems,routine inspections using drones equipped with advanced object detection algorithms are essential for preempting power-related issues.The increasing resolution of drone-cap... To maintain the reliability of power systems,routine inspections using drones equipped with advanced object detection algorithms are essential for preempting power-related issues.The increasing resolution of drone-captured images has posed a challenge for traditional target detection methods,especially in identifying small objects in high-resolution images.This study presents an enhanced object detection algorithm based on the Faster Regionbased Convolutional Neural Network(Faster R-CNN)framework,specifically tailored for detecting small-scale electrical components like insulators,shock hammers,and screws in transmission line.The algorithm features an improved backbone network for Faster R-CNN,which significantly boosts the feature extraction network’s ability to detect fine details.The Region Proposal Network is optimized using a method of guided feature refinement(GFR),which achieves a balance between accuracy and speed.The incorporation of Generalized Intersection over Union(GIOU)and Region of Interest(ROI)Align further refines themodel’s accuracy.Experimental results demonstrate a notable improvement in mean Average Precision,reaching 89.3%,an 11.1%increase compared to the standard Faster R-CNN.This highlights the effectiveness of the proposed algorithm in identifying electrical components in high-resolution aerial images. 展开更多
关键词 Faster R-CNN transmission line object detection GIOU GFR
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利用籼粳交RILs群体定位水稻秧苗素质性状QTL
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作者 刘丹 刘进 +6 位作者 冯章丽 王嘉宇 隋常玲 邓力喜 冯博 杨林林 娄惠婕 《分子植物育种》 北大核心 2025年第6期1856-1863,共8页
秧苗素质影响水稻移栽返青和苗期长势,壮秧对水稻产量和品质的形成具有至关重要的作用。为了解析秧苗素质相关性状的遗传机理,本研究以粳型超级稻品种‘龙稻5’和典型籼稻品种‘中优早8’为亲本构建的重组自交系群体为试验材料,测定秧... 秧苗素质影响水稻移栽返青和苗期长势,壮秧对水稻产量和品质的形成具有至关重要的作用。为了解析秧苗素质相关性状的遗传机理,本研究以粳型超级稻品种‘龙稻5’和典型籼稻品种‘中优早8’为亲本构建的重组自交系群体为试验材料,测定秧苗株高(SH)、总根数(TRN)、茎基宽(BCW)、根鲜重(FRW)和茎叶鲜重(FSLW)等9个秧苗素质相关性状,进行表型分析和QTL(quantitative trait locus)定位。结果表明,亲本间秧苗素质存在明显差异‘,中优早8’具有较好的早生快发特性,综合秧苗素质强于‘龙稻5’;RIL(recombinant inbred lines)群体中秧苗素质相关指标间存在较大变异,呈现双向超亲分离,符合连续的正态分布;除根冠比和茎基宽株高比性状外,其他不同指标间均呈现正相关关系。共检测到8个控制秧苗素质性状的QTL,包括1个总根数QTL、1个根鲜重QTL、3个根冠比QTL和3个茎基宽株高比QTL,分布于第1、2、3、4、5和10号染色体上,QTL的贡献率在2.98~6.34%之间。研究结果将为深入挖掘和克隆水稻秧苗素质性状相关基因提供理论基础。 展开更多
关键词 水稻 重组自交系 秧苗素质 QTL定位
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基于自适应EDLines和鲁棒位姿优化的视觉SLAM算法
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作者 王浩森 张丽杰 《计算机仿真》 2025年第9期355-361,共7页
同时定位与地图构建(SLAM)广泛应用于无人驾驶、机器人等多个领域,为了提高ORB-SLAM3在光照不均和弱纹理场景中的定位精度和鲁棒性,提出了一种基于自适应EDLines和鲁棒位姿优化的视觉SLAM算法。首先,提出了一种自适应EDLines线段检测算... 同时定位与地图构建(SLAM)广泛应用于无人驾驶、机器人等多个领域,为了提高ORB-SLAM3在光照不均和弱纹理场景中的定位精度和鲁棒性,提出了一种基于自适应EDLines和鲁棒位姿优化的视觉SLAM算法。首先,提出了一种自适应EDLines线段检测算法,根据像素梯度的变化更新梯度阈值,并设计相似线段删除和分割线段合并策略,提高EDLines提取线特征的准确性和完整性。其次,提出一种与线段端点无关的线特征残差计算方法,避免后端优化过程中不同视角对位姿估计结果的影响。实验结果表明,提出的视觉SLAM算法比ORB-SLAM3具有更高的定位精度和鲁棒性。 展开更多
关键词 同时定位与地图构建 线特征 线特征提取 位姿优化
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