Objective： Laser capture microdisection has become indispensable to the analysis of the difference of gene expression between human bladder transitional cell and bladder transitional cell carcinoma （BTCC）. However,...Objective： Laser capture microdisection has become indispensable to the analysis of the difference of gene expression between human bladder transitional cell and bladder transitional cell carcinoma （BTCC）. However, to obtain sufficient RNA from laser-capture microdissected cells is quite difficult. The study was designed to determinc a feasible technical routine to isolate transitional cells from bladder membrane, separate carcinoma cclls from stromal cells and to amplify the RNA isolated from laser-capture microdissected cells. Methods： Bladder transitional cell were obtained from frozen sections of bladder membrane applying LCM, by the same token, BTCC cells from frozen sections of BTCC tissue. Then RNA was extracted and linearly amplified in vitro. The expression levels of β-actin in primary total RNA and amplified RNA were detected using RT-PCR. Results： That RNA integrity was good after LCM was confirmed by control experiment Ⅰ; By control experiment Ⅱ, the correlation between the number of LCM-shooting and RNA quantity undcr arranged conditions was preliminarily confirmed. About 0.5-2.5kb RNA fragments were obtained after RNA amplification and β-actin levels were integral. Conclusion： Laser capture microdissection combined with RNA linear amplification in vitro can be successfully applied to obtain pure objective cells for research. The integrity of the amplified RNA is good and can be employed in further research.展开更多
Noiseless linear amplification （NLA）, first proposed by Ralpha et al., is a nondeterministic amplification process which gives gain to the Fock state ｜n） → gn｜n）, with g being the amplification gain. We here gi...Noiseless linear amplification （NLA）, first proposed by Ralpha et al., is a nondeterministic amplification process which gives gain to the Fock state ｜n） → gn｜n）, with g being the amplification gain. We here give a general frame- work for improving the NLA scheme with arbitrary general local unitary operations. We derive the improvement in the amplification gain in 0 1 photon subspace. In particular, we study if the local unitary is composed of sin- gle mode squeezing and coherent displacement operation. Finally, numerical simulations show that local unitary operation could give a further enhancement in the amplification gain as well as the success probability, making the NLA more feasible in future optic quantum communications.展开更多
Quantum states are essential resource for quantum-enhanced applications.Loss incurred in the distribution channel,however,dissipates the high signal-to-noise ratio advantage enjoyed by the squeezed state.Here,we first...Quantum states are essential resource for quantum-enhanced applications.Loss incurred in the distribution channel,however,dissipates the high signal-to-noise ratio advantage enjoyed by the squeezed state.Here,we first demonstrate noise immunity enhancement by using phase-sensitive amplifier(PSA)with measurement-based noiseless linear amplifier(MB-NLA).We explore the signal transfer capability with the amplifier in a noisy channel.The MB-NLA enhanced PSA has obvious suppression effect on channel noises,especially it has improvement for the noise contaminated signal.Better performance can be achieved by flexibly adjusting amplifier parameters.With the amplifier,it is promising to overcome the entanglement-distribution loss and show its superiority in squeezing based quantum sensing.展开更多
In order to overcome the bottleneck of low linearity and low resolution, an improved delay line structure is proposed with a calibration algorithm to conquer PVT （process, voltage and temperature） variations for an ...In order to overcome the bottleneck of low linearity and low resolution, an improved delay line structure is proposed with a calibration algorithm to conquer PVT （process, voltage and temperature） variations for an all- digital design. The chip is implemented in 0.13 μm CMOS technology. Measurement results show that the proposed structure with the calibration algorithm can evidently improve the linearity and resolution of the delay line. The delay resolution is 2 ps and the root mean square jitter of the delay is 4.71 ps, leading to an error vector magnitude enhancement of 1.32 dB.展开更多
The linear amplification with nonlinear component transmitter is a promising solution to high efficiency and high linearity amplification for non-constant envelope signals. An all-digital synthesizable baseband for a ...The linear amplification with nonlinear component transmitter is a promising solution to high efficiency and high linearity amplification for non-constant envelope signals. An all-digital synthesizable baseband for a delay-based LINC transmitter is implemented. This paper proposes a standard-cell based synthesizable methodology which can be applied in the ASIC process efficiently without performance degradation compared to the manual layout. A scheme to overcome the limited resolution of conventional phase detectors is proposed. It employs alter- native phase detector structures to provide reconfigurability for higher resolution after fabricating, resulting in an 11 ps resolution improvement. Due to the PVT variation, an adaptive calibration scheme focusing on the inherent imbalance between two delay lines is depicted, which reveals an effective EVM enhancement of 5.37 dB. This baseband chip is implemented in 0.13 μm CMOS technology, and the transmitter with the baseband has an EVM of-28.96 dB and an ACPR of-29.51 dB, meeting the design requirement.展开更多
文摘Objective： Laser capture microdisection has become indispensable to the analysis of the difference of gene expression between human bladder transitional cell and bladder transitional cell carcinoma （BTCC）. However, to obtain sufficient RNA from laser-capture microdissected cells is quite difficult. The study was designed to determinc a feasible technical routine to isolate transitional cells from bladder membrane, separate carcinoma cclls from stromal cells and to amplify the RNA isolated from laser-capture microdissected cells. Methods： Bladder transitional cell were obtained from frozen sections of bladder membrane applying LCM, by the same token, BTCC cells from frozen sections of BTCC tissue. Then RNA was extracted and linearly amplified in vitro. The expression levels of β-actin in primary total RNA and amplified RNA were detected using RT-PCR. Results： That RNA integrity was good after LCM was confirmed by control experiment Ⅰ; By control experiment Ⅱ, the correlation between the number of LCM-shooting and RNA quantity undcr arranged conditions was preliminarily confirmed. About 0.5-2.5kb RNA fragments were obtained after RNA amplification and β-actin levels were integral. Conclusion： Laser capture microdissection combined with RNA linear amplification in vitro can be successfully applied to obtain pure objective cells for research. The integrity of the amplified RNA is good and can be employed in further research.
基金Supported by the National Natural Science Foundation of China under Grant Nos 11304013,11204197,11204379 and 11074244the National Basic Research Program of China under Grant No 2011CBA00200+1 种基金the Doctor Science Research Foundation of Ministry of Education of China under Grant No 20113402110059Civil Aerospace 2013669
文摘Noiseless linear amplification （NLA）, first proposed by Ralpha et al., is a nondeterministic amplification process which gives gain to the Fock state ｜n） → gn｜n）, with g being the amplification gain. We here give a general frame- work for improving the NLA scheme with arbitrary general local unitary operations. We derive the improvement in the amplification gain in 0 1 photon subspace. In particular, we study if the local unitary is composed of sin- gle mode squeezing and coherent displacement operation. Finally, numerical simulations show that local unitary operation could give a further enhancement in the amplification gain as well as the success probability, making the NLA more feasible in future optic quantum communications.
基金the National Natural Science Foundation of China(Grant No.12074233)the National Key Research and Development Program of China(Grant No.2021YFC2201802)Shanxi(1331 Project)。
文摘Quantum states are essential resource for quantum-enhanced applications.Loss incurred in the distribution channel,however,dissipates the high signal-to-noise ratio advantage enjoyed by the squeezed state.Here,we first demonstrate noise immunity enhancement by using phase-sensitive amplifier(PSA)with measurement-based noiseless linear amplifier(MB-NLA).We explore the signal transfer capability with the amplifier in a noisy channel.The MB-NLA enhanced PSA has obvious suppression effect on channel noises,especially it has improvement for the noise contaminated signal.Better performance can be achieved by flexibly adjusting amplifier parameters.With the amplifier,it is promising to overcome the entanglement-distribution loss and show its superiority in squeezing based quantum sensing.
文摘In order to overcome the bottleneck of low linearity and low resolution, an improved delay line structure is proposed with a calibration algorithm to conquer PVT （process, voltage and temperature） variations for an all- digital design. The chip is implemented in 0.13 μm CMOS technology. Measurement results show that the proposed structure with the calibration algorithm can evidently improve the linearity and resolution of the delay line. The delay resolution is 2 ps and the root mean square jitter of the delay is 4.71 ps, leading to an error vector magnitude enhancement of 1.32 dB.
文摘The linear amplification with nonlinear component transmitter is a promising solution to high efficiency and high linearity amplification for non-constant envelope signals. An all-digital synthesizable baseband for a delay-based LINC transmitter is implemented. This paper proposes a standard-cell based synthesizable methodology which can be applied in the ASIC process efficiently without performance degradation compared to the manual layout. A scheme to overcome the limited resolution of conventional phase detectors is proposed. It employs alter- native phase detector structures to provide reconfigurability for higher resolution after fabricating, resulting in an 11 ps resolution improvement. Due to the PVT variation, an adaptive calibration scheme focusing on the inherent imbalance between two delay lines is depicted, which reveals an effective EVM enhancement of 5.37 dB. This baseband chip is implemented in 0.13 μm CMOS technology, and the transmitter with the baseband has an EVM of-28.96 dB and an ACPR of-29.51 dB, meeting the design requirement.
