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A Q-Learning Improved Particle Swarm Optimization for Aircraft Pulsating Assembly Line Scheduling Problem Considering Skilled Operator Allocation
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作者 Xiaoyu Wen Haohao Liu +6 位作者 Xinyu Zhang Haoqi Wang Yuyan Zhang Guoyong Ye Hongwen Xing Siren Liu Hao Li 《Computers, Materials & Continua》 2026年第1期1503-1529,共27页
Aircraft assembly is characterized by stringent precedence constraints,limited resource availability,spatial restrictions,and a high degree of manual intervention.These factors lead to considerable variability in oper... Aircraft assembly is characterized by stringent precedence constraints,limited resource availability,spatial restrictions,and a high degree of manual intervention.These factors lead to considerable variability in operator workloads and significantly increase the complexity of scheduling.To address this challenge,this study investigates the Aircraft Pulsating Assembly Line Scheduling Problem(APALSP)under skilled operator allocation,with the objective of minimizing assembly completion time.A mathematical model considering skilled operator allocation is developed,and a Q-Learning improved Particle Swarm Optimization algorithm(QLPSO)is proposed.In the algorithm design,a reverse scheduling strategy is adopted to effectively manage large-scale precedence constraints.Moreover,a reverse sequence encoding method is introduced to generate operation sequences,while a time decoding mechanism is employed to determine completion times.The problem is further reformulated as a Markov Decision Process(MDP)with explicitly defined state and action spaces.Within QLPSO,the Q-learning mechanism adaptively adjusts inertia weights and learning factors,thereby achieving a balance between exploration capability and convergence performance.To validate the effectiveness of the proposed approach,extensive computational experiments are conducted on benchmark instances of different scales,including small,medium,large,and ultra-large cases.The results demonstrate that QLPSO consistently delivers stable and high-quality solutions across all scenarios.In ultra-large-scale instances,it improves the best solution by 25.2%compared with the Genetic Algorithm(GA)and enhances the average solution by 16.9%over the Q-learning algorithm,showing clear advantages over the comparative methods.These findings not only confirm the effectiveness of the proposed algorithm but also provide valuable theoretical references and practical guidance for the intelligent scheduling optimization of aircraft pulsating assembly lines. 展开更多
关键词 Aircraft pulsating assembly lines skilled operator reinforcement learning PSO reverse scheduling
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Deep Learning-Based Toolkit Inspection:Object Detection and Segmentation in Assembly Lines
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作者 Arvind Mukundan Riya Karmakar +1 位作者 Devansh Gupta Hsiang-Chen Wang 《Computers, Materials & Continua》 2026年第1期1255-1277,共23页
Modern manufacturing processes have become more reliant on automation because of the accelerated transition from Industry 3.0 to Industry 4.0.Manual inspection of products on assembly lines remains inefficient,prone t... Modern manufacturing processes have become more reliant on automation because of the accelerated transition from Industry 3.0 to Industry 4.0.Manual inspection of products on assembly lines remains inefficient,prone to errors and lacks consistency,emphasizing the need for a reliable and automated inspection system.Leveraging both object detection and image segmentation approaches,this research proposes a vision-based solution for the detection of various kinds of tools in the toolkit using deep learning(DL)models.Two Intel RealSense D455f depth cameras were arranged in a top down configuration to capture both RGB and depth images of the toolkits.After applying multiple constraints and enhancing them through preprocessing and augmentation,a dataset consisting of 3300 annotated RGB-D photos was generated.Several DL models were selected through a comprehensive assessment of mean Average Precision(mAP),precision-recall equilibrium,inference latency(target≥30 FPS),and computational burden,resulting in a preference for YOLO and Region-based Convolutional Neural Networks(R-CNN)variants over ViT-based models due to the latter’s increased latency and resource requirements.YOLOV5,YOLOV8,YOLOV11,Faster R-CNN,and Mask R-CNN were trained on the annotated dataset and evaluated using key performance metrics(Recall,Accuracy,F1-score,and Precision).YOLOV11 demonstrated balanced excellence with 93.0%precision,89.9%recall,and a 90.6%F1-score in object detection,as well as 96.9%precision,95.3%recall,and a 96.5%F1-score in instance segmentation with an average inference time of 25 ms per frame(≈40 FPS),demonstrating real-time performance.Leveraging these results,a YOLOV11-based windows application was successfully deployed in a real-time assembly line environment,where it accurately processed live video streams to detect and segment tools within toolkits,demonstrating its practical effectiveness in industrial automation.The application is capable of precisely measuring socket dimensions by utilising edge detection techniques on YOLOv11 segmentation masks,in addition to detection and segmentation.This makes it possible to do specification-level quality control right on the assembly line,which improves the ability to examine things in real time.The implementation is a big step forward for intelligent manufacturing in the Industry 4.0 paradigm.It provides a scalable,efficient,and accurate way to do automated inspection and dimensional verification activities. 展开更多
关键词 Tool detection image segmentation object detection assembly line automation Industry 4.0 Intel RealSense deep learning toolkit verification RGB-D imaging quality assurance
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GFRF R-CNN:Object Detection Algorithm for Transmission Lines
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作者 Xunguang Yan Wenrui Wang +3 位作者 Fanglin Lu Hongyong Fan Bo Wu Jianfeng Yu 《Computers, Materials & Continua》 SCIE EI 2025年第1期1439-1458,共20页
To maintain the reliability of power systems,routine inspections using drones equipped with advanced object detection algorithms are essential for preempting power-related issues.The increasing resolution of drone-cap... To maintain the reliability of power systems,routine inspections using drones equipped with advanced object detection algorithms are essential for preempting power-related issues.The increasing resolution of drone-captured images has posed a challenge for traditional target detection methods,especially in identifying small objects in high-resolution images.This study presents an enhanced object detection algorithm based on the Faster Regionbased Convolutional Neural Network(Faster R-CNN)framework,specifically tailored for detecting small-scale electrical components like insulators,shock hammers,and screws in transmission line.The algorithm features an improved backbone network for Faster R-CNN,which significantly boosts the feature extraction network’s ability to detect fine details.The Region Proposal Network is optimized using a method of guided feature refinement(GFR),which achieves a balance between accuracy and speed.The incorporation of Generalized Intersection over Union(GIOU)and Region of Interest(ROI)Align further refines themodel’s accuracy.Experimental results demonstrate a notable improvement in mean Average Precision,reaching 89.3%,an 11.1%increase compared to the standard Faster R-CNN.This highlights the effectiveness of the proposed algorithm in identifying electrical components in high-resolution aerial images. 展开更多
关键词 Faster R-CNN transmission line object detection GIOU GFR
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Rail Line Detection Algorithm Based on Improved CLRNet
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作者 ZHOU Bowei XING Guanyu LIU Yanli 《Journal of Shanghai Jiaotong university(Science)》 2025年第5期923-934,共12页
In smart driving for rail transit,a reliable obstacle detection system is an important guarantee for the safety of trains.Therein,the detection of the rail area directly affects the accuracy of the system to identify ... In smart driving for rail transit,a reliable obstacle detection system is an important guarantee for the safety of trains.Therein,the detection of the rail area directly affects the accuracy of the system to identify dangerous targets.Both the rail line and the lane are presented as thin line shapes in the image,but the rail scene is more complex,and the color of the rail line is more difficult to distinguish from the background.By comparison,there are already many deep learning-based lane detection algorithms,but there is a lack of public datasets and targeted deep learning detection algorithms for rail line detection.To address this,this paper constructs a rail image dataset RailwayLine and labels the rail line for the training and testing of models.This dataset contains rich rail images including single-rail,multi-rail,straight rail,curved rail,crossing rails,occlusion,blur,and different lighting conditions.To address the problem of the lack of deep learning-based rail line detection algorithms,we improve the CLRNet algorithm which has an excellent performance in lane detection,and propose the CLRNet-R algorithm for rail line detection.To address the problem of the rail line being thin and occupying fewer pixels in the image,making it difficult to distinguish from complex backgrounds,we introduce an attention mechanism to enhance global feature extraction ability and add a semantic segmentation head to enhance the features of the rail region by the binary probability of rail lines.To address the poor curve recognition performance and unsmooth output lines in the original CLRNet algorithm,we improve the weight allocation for line intersection-over-union calculation in the original framework and propose two loss functions based on local slopes to optimize the model’s local sampling point training constraints,improving the model’s fitting performance on curved rails and obtaining smooth and stable rail line detection results.Through experiments,this paper demonstrates that compared with other mainstream lane detection algorithms,the algorithm proposed in this paper has a better performance for rail line detection. 展开更多
关键词 rail line detection attention mechanism semantic segmentation loss function CLRNet algorithm
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Retraction:Puerarin inhibits proliferation and induces apoptosis by upregulation of miR-16 in bladder cancer cell line T24
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作者 Oncology Research Editorial Office 《Oncology Research》 2025年第4期993-993,共1页
The published article titled“Puerarin inhibits proliferation and induces apoptosis by upregulation of miR-16 in bladder cancer cell line T24”has been retracted from Oncology Research,Vol.26,No.8,2018,pp.1227–1234.
关键词 PUERARIN bladder cancer bladder cancer cell line T miR APOPTOSIS PROLIFERATION
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Research on the Optimization and Simulation of Assembly Line Balancing Based on Improved PSO Algorithm
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作者 Wenkang Zhang 《Journal of World Architecture》 2025年第3期159-168,共10页
In response to the deficiencies of commonly used optimization methods for assembly lines,a production demand-oriented optimization method for assembly lines is proposed.Taking a certain compressor assembly line as an ... In response to the deficiencies of commonly used optimization methods for assembly lines,a production demand-oriented optimization method for assembly lines is proposed.Taking a certain compressor assembly line as an example,the production rhythm and the number of workstations are calculated based on production requirements and working systems.With assembly rhythm and smoothing index as optimization goals,an improved particle swarm optimization algorithm is employed for process allocation.Subsequently,Flexsim simulation is used to analyze the assembly line.The final results show that after optimization using the improved particle swarm algorithm,the assembly line balance rate increased from 71.1%to 85.9%,and the assembly line smoothing index decreased from 47.4 to 29.8,significantly enhancing assembly efficiency.This demonstrates the effectiveness of the proposed optimization method for the assembly line and provides a reference for other products in the same industry. 展开更多
关键词 Assembly line balance Improve PSO Simulation optimization
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Optically-excited acoustic waves in Si nanowires probed by time-resolved HOLZ lines
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作者 He Wang Shuaishuai Sun +7 位作者 Yizhe Wang Qianming An Xianhui Ye Jun Li Huanfang Tian Huaixin Yang Jianqi Li Zian Li 《Chinese Physics B》 2025年第9期663-669,共7页
Exploring advanced techniques capable of probing nanometric acoustic waves in nanostructures is critically important for the development of miniaturized acoustic devices.In this study,we probe the optically-excited ac... Exploring advanced techniques capable of probing nanometric acoustic waves in nanostructures is critically important for the development of miniaturized acoustic devices.In this study,we probe the optically-excited acoustic waves in a single silicon nanowire(NW)using the time-resolved(tr-)high-order Laue-zone(HOLZ)lines under convergent-beam electron diffraction(CBED)conditions in an ultrafast transmission electron microscope(UTEM).We devise an experimental scheme to obtain tr-HOLZ lines under off-zone-axis CBED conditions.We also propose a geometric description of HOLZ line formation and use this alternative description to quantitatively evaluate the dynamics of optically-excited silicon NW.Using part of the deformation gradient tensor,our simulations of the dynamics of Si NW reproduce the experimental results.We further discuss the feasibility of a full retrieval of the deformation gradient tensor by using a set of HOLZ lines from three zone axes.Our findings illustrate a strategy for the quantitative access to dynamical acoustic waves optically excited in micro-and nano-structures using UTEM. 展开更多
关键词 ultrafast transmission electron microscope high-order Laue-zone lines pulsed laser excitation acoustic waves
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MAID:making accurate transmission line icing detector by enhancing inaccurate dataset
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作者 SUN Wei WANG Yu +3 位作者 GAO Bo ZHANG Shujuan WANG Xiaojin XING Lu 《Optoelectronics Letters》 2025年第10期606-611,共6页
Power transmission lines are a critical component of the entire power system,and ice accretion incidents caused by various types of power systems can result in immeasurable harm.Currently,network models used for ice d... Power transmission lines are a critical component of the entire power system,and ice accretion incidents caused by various types of power systems can result in immeasurable harm.Currently,network models used for ice detection on power transmission lines require a substantial amount of sample data to support their training,and their drawback is that detection accuracy is significantly affected by the inaccurate annotation among training dataset.Therefore,we propose a transformer-based detection model,structured into two stages to collectively address the impact of inaccurate datasets on model training.In the first stage,a spatial similarity enhancement(SSE)module is designed to leverage spatial information to enhance the construction of the detection framework,thereby improving the accuracy of the detector.In the second stage,a target similarity enhancement(TSE)module is introduced to enhance object-related features,reducing the impact of inaccurate data on model training,thereby expanding global correlation.Additionally,by incorporating a multi-head adaptive attention window(MAAW),spatial information is combined with category information to achieve information interaction.Simultaneously,a quasi-wavelet structure,compatible with deep learning,is employed to highlight subtle features at different scales.Experimental results indicate that the proposed model in this paper outperforms existing mainstream detection models,demonstrating superior performance and stability. 展开更多
关键词 power transmission lines ice detection ice accretion sample data spatial similarity enhancement power systemand transformer based model power systems
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Influence of Cy berKnife Prescription Isodose Line on the Discrepancy of Dose Results Calculated by the Ray Tracing and Monte Carlo Algorithms for Head and Lung Plans: A Phantom Study 被引量:2
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作者 Jing YANG Gang LIU +5 位作者 Hong-yuan LIU Xin NIE Zhi-yong YANG Jun HAN Sheng ZHANG Zhi-wen LIANG 《Current Medical Science》 SCIE CAS 2020年第2期301-306,共6页
Incorporation of the Monte Carlo(MC)algorithm in optimizing CyberKnife(CK)plans is cumbersome,and early models unconfigured MC calculations,therefore,this study investigated algorithm-based dose calculation discrepanc... Incorporation of the Monte Carlo(MC)algorithm in optimizing CyberKnife(CK)plans is cumbersome,and early models unconfigured MC calculations,therefore,this study investigated algorithm-based dose calculation discrepancies by selecting different prescription isodose lines(PIDLs)in head and lung CK plans.CK plans were based on anthropomorphic phantoms.Four shells were set at 2-60 mm from the target,and the constraint doses were adjusted according to the design stratcgy.After optimization,30%-90%PIDL plans were generated by ray tracing(RT).In the evaluation module,CK plans were recalculated using the MC algorithm.Therefore,the dosimetric parameters of different PIDL plans based on the RT and MC algorithms were obtained and analyzed.The discrepancies(mean+SD)were 3.72%+0.31%,3.40%+0.11%,3.47%+0.32%,0.17%+0.11%,0.64%+3.60%,7.73%+1.60%,14.62%+3.21%and 10.10%+1.57%for Djs,Dmeam),Dys,and coverage of the PTV,DGI,V,,V;and V,in the head plans and-6.32%+1.15%,-13.46%+0.98%,-20.63%+2.25%,-34.78%+25.03%,12248%+175.60%,-12.92%+5.41%,3.19%+4.67%and 7.13%+1.56%in the lung plans,respectively.The following parameters were significantly correlated with PIDL:dp98%at the 0.05 level and dpal,dys and dv3 at the 0.01 level for the head plans;dp98e%at the 0.05 level and do1e%,dpmeam,Ccoweange,dool,dvs and dv;at the 0.01 level for the lung plans.RT may be used to calculate the dose in CK head plans,but when the dose of organs at risk is close to the limit,it is necessary to refer to the MC results or to further optimize the CK plan to reduce the dose.For lung plans,the MC algorithm is recommended.For early models without the MC algorithm,a lower PIDL plan is recommended;otherwise,a large PIDL plan risks serious underdosage in the target area. 展开更多
关键词 CYBERKNIFE prescription isodose line Monte Carlo ray tracing PHANTOM
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Self-adaptive PID controller of microwave drying rotary device tuning on-line by genetic algorithms 被引量:6
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作者 杨彪 梁贵安 +5 位作者 彭金辉 郭胜惠 李玮 张世敏 李英伟 白松 《Journal of Central South University》 SCIE EI CAS 2013年第10期2685-2692,共8页
The control design, based on self-adaptive PID with genetic algorithms(GA) tuning on-line was investigated, for the temperature control of industrial microwave drying rotary device with the multi-layer(IMDRDWM) and wi... The control design, based on self-adaptive PID with genetic algorithms(GA) tuning on-line was investigated, for the temperature control of industrial microwave drying rotary device with the multi-layer(IMDRDWM) and with multivariable nonlinear interaction of microwave and materials. The conventional PID control strategy incorporated with optimization GA was put forward to maintain the optimum drying temperature in order to keep the moisture content below 1%, whose adaptation ability included the cost function of optimization GA according to the output change. Simulations on five different industrial process models and practical temperature process control system for selenium-enriched slag drying intensively by using IMDRDWM were carried out systematically, indicating the reliability and effectiveness of control design. The parameters of proposed control design are all on-line implemented without iterative predictive calculations, and the closed-loop system stability is guaranteed, which makes the developed scheme simpler in its synthesis and application, providing the practical guidelines for the control implementation and the parameter design. 展开更多
关键词 industrial microwave DRYING ROTARY device SELF-ADAPTIVE PID controller genetic algorithm ON-line tuning SELENIUM-ENRICHED SLAG
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On the Solution of the Eigenvalue Complementarity Problem by a Line Search Filter-SQP Algorithm
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作者 Qiu Yu Zhensheng Yu Yangchen Liu 《Journal of Applied Mathematics and Physics》 2017年第10期1986-1996,共11页
In this paper, the Eigenvalue Complementarity Problem (EiCP) with real symmetric matrices is addressed, which appears in the study of contact problem in mechanics. We discuss a quadratic programming formulation to the... In this paper, the Eigenvalue Complementarity Problem (EiCP) with real symmetric matrices is addressed, which appears in the study of contact problem in mechanics. We discuss a quadratic programming formulation to the problem. The resulting problems are nonlinear programs that can be solved by a line search filter-SQP algorithm. 展开更多
关键词 EIGENVALUE Complementarity PROBLEM Nonlinear PROGRAMMING line SEARCH FILTER Method
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The Construction of Heat-resistant Near Isogenic Line (NIL) of Bombyx mori and the Development of Molecular Marker Breeding Technique 被引量:5
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作者 赵远 吴阳春 张健 《Agricultural Science & Technology》 CAS 2010年第1期92-95,共4页
[Objective] The study aimed to provide theoretical basis for development and application of molecular marker breeding technique to obtain Bombyx mori near-isogenic lines (NILs). [Method] Thermotolerance gene was int... [Objective] The study aimed to provide theoretical basis for development and application of molecular marker breeding technique to obtain Bombyx mori near-isogenic lines (NILs). [Method] Thermotolerance gene was introduced into sensitive variety Ou17 by developing NILs and recurrent backcross,then through six generations of backcross,thermotolerance-assisted selection,and two generations of self-cross. [Result] Bombyx mori NILs carrying thermotolerance gene (new germplasm) were produced. Meanwhile,thermotolerance level of progenies of each backcross and molecular markers of NILs were determined,and then attempts were made to produce practical thermotolerance hybrids by using thermotolerance varieties whose thermotolerance gene is linked to SSR markers. [Conclusion] The study successfully construct thermotolerance NILs,monitor thermotolerance level and breeding results of progenies of each backcross,and determine molecular marker of NILs. 展开更多
关键词 Silkworm species CULTIVATION Near-isogenic lines SSR molecular marker
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Screening of the Metastasis-Associated Genes by Gene Chip in High Metastatic Human Ovarian Cancer Cell Lines 被引量:1
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作者 许沈华 牟瀚舟 +3 位作者 顾琳慧 苏丹 朱赤红 刘祥麟 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2007年第7期581-590,共10页
Affymetrix U133A oligonucleotide microarrays were used to study the differences of gene expressions between high (H) metastatic ovarian cancer cell line, HO-8910PM, and normal ovarian tissues (C). Bioinformatics w... Affymetrix U133A oligonucleotide microarrays were used to study the differences of gene expressions between high (H) metastatic ovarian cancer cell line, HO-8910PM, and normal ovarian tissues (C). Bioinformatics was used to identify their chromosomal localizations. A total of 1,237 genes were found to have a difference in expression levels more than eight times. Among them 597 were upregulated [Signal Log Ratio (SLR) ≥3], and 640 genes were downregulated (SLR≤-3). Except one gene, whose location was unknown, all these genes were randomly distributed on all the chromosomes. However, chromosome 1 contained the most differentially expressed genes (115 genes, 9.3%), followed by chromosome 2 (94 genes, 7.6%), chromosome 12 (88 genes, 7.1%), chromosome 11 (76 genes, 6.1%), chromosomes X (71 genes, 5.7%), and chromosomes l7 (69 genes, 5.6%). These genes were localized on short-arm of chromosome (q), which had 805 (65.1%) genes, and the short arms of No.13, 14, 15, 21, and 22 chromosomes were the only parts of the chromosomes where the differentially expressed genes were localized. Functional classification showed that most of the genes (306 genes, 24.7%) belonged to the enzymes and their regulator groups. The subsequent group was the nucleic acid binding genes (144 genes, 11.6%). The rest of the top two groups were signal transduction genes (137 genes, 11.1%) and proteins binding genes (116 genes, 9.4%). These comprised 56.8% of all the differentially expressed genes. There were also 207 genes whose functions were unknown (16.7 %). Therefore it was concluded that differentially expressed genes in high metastatic ovarian cancer cell were supposed to be randomly distributed across the genome, but the majority were found on chromosomes 1, 2, 12, 11, 17, and X. Abnormality in four groups of genes, including in enzyme and its regulator, nucleic acid binding, signal transduction and protein binding associated genes, might play important roles in ovarian cancer metastasis. Those genes need to be further studied. 展开更多
关键词 ovarian cancer cell line metastatic associated gene chromosomal localization molecular function
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非平衡大气辐射强度与透过率的Line-By-Line计算模式 被引量:1
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作者 朱世德 向安平 《计算物理》 CSCD 北大核心 1998年第5期110-118,共9页
导出了非局域热力学平衡(NLTE)大气辐射强度与透过率的基本公式,以及NLT和LTE的线强、光学厚度和透过率的关系。假设转动能级是热力学平衡(LTE)的,建立了一个精确的LinebyLineNLTE辐射强度与透过... 导出了非局域热力学平衡(NLTE)大气辐射强度与透过率的基本公式,以及NLT和LTE的线强、光学厚度和透过率的关系。假设转动能级是热力学平衡(LTE)的,建立了一个精确的LinebyLineNLTE辐射强度与透过率的有效算法来计算较高层行星大气红外活跃样品的辐射强度。计算了地球大气16O396μm带在白天、夜晚和NLTE、LTE条件下的临边光谱辐射强度、线积分辐射强度、带积分辐射强度和合成光谱辐射强度,揭示了NLTE辐射强度对LTE辐射强度有显著而重要的偏离。 展开更多
关键词 非局域热力学平衡(NLTE) 发射与透射 辐射强度与透过率 line-by-line算法
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IMPROVEMENTS IN HIDDEN LINE REMOVAL ALGORITHM FOR BI-PARAMETRIC SURFACES
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作者 李卫国 唐月红 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 1998年第2期93-97,共5页
A hidden line removal algorithm for bi parametric surfaces is presented and illustrated by some experimental results. The enclosure test is done using area coordinates. A technique of moving box of encirclement is p... A hidden line removal algorithm for bi parametric surfaces is presented and illustrated by some experimental results. The enclosure test is done using area coordinates. A technique of moving box of encirclement is presented. It is found that the algorithm is of general purpose, requires minimal computer storage, has high accuracy and simplicity, and is very easy to be implemented on a computer. 展开更多
关键词 computational geometry computer graphics computer aided design hidden line removal area coordinates bi parametric surfaces
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Characterization of Two Human Lung Adenocarcinoma Cell Lines by Reciprocal Chromosome Painting 被引量:4
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作者 彭昆靖 王金焕 +3 位作者 苏伟婷 王熙才 杨凤堂 佴文惠 《Zoological Research》 CAS CSCD 北大核心 2010年第2期113-121,共9页
Lung cancer is a leading cause of cancer death worldwide. Some lung cancer patients correlate with a gas of radon besides smoking. To search for common chromosomal aberrations in lung cancer cell lines established fro... Lung cancer is a leading cause of cancer death worldwide. Some lung cancer patients correlate with a gas of radon besides smoking. To search for common chromosomal aberrations in lung cancer cell lines established from patients induced by different factors, a combined approach of chromosome sorting, forward and reverse chromosome painting was used to characterize karyotypes of two lung adenocarcinoma cell lines: A549 and GLC-82 with the latter line derived from a patient who has suffered long-term exposure to environmental radon gas pollution. The chromosome painting results revealed that complex chromosomal rearrangements occurred in these two lung adenocarcinoma cell lines. Thirteen and twenty-four abnormal chromosomes were identified An A549 and GLC-82 cell lines, respectively. Almost half of abnormal chromosomes in these two cell lines were formed by non-reciprocal translocations, the others were derived from deletions and duplication/or amplification in some chromosomal regions. Furthermore, two apparently common breakpoints, HSA8q24 and 12q14 were found in these two lung cancer cell lines. 展开更多
关键词 Lung adenocarcinoma cell lines Chromosome sorting Chromosome painting Cytogenetic characterization
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基于转座元件分析揭示绵羊基因组中LINE转座子元件的特性和进化
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作者 梁千千 董华娇 +4 位作者 李甜甜 张洁 杨敏 万鹏程 韩吉龙 《内蒙古农业大学学报(自然科学版)》 北大核心 2025年第6期1-8,共8页
本研究采用不同的3种方法对绵羊基因组进行注释,筛选出适合绵羊转座元件注释的方法,并进行转座元件的演化历史分析。结果表明,采用从头预测(de novo prediction)和同源预测(homology?based prediction)相结合的策略对绵羊基因组进行注... 本研究采用不同的3种方法对绵羊基因组进行注释,筛选出适合绵羊转座元件注释的方法,并进行转座元件的演化历史分析。结果表明,采用从头预测(de novo prediction)和同源预测(homology?based prediction)相结合的策略对绵羊基因组进行注释效果最佳,鉴定出绵羊基因组中转座元件占比为47.34%,其中长散布核元件(LINE)占比最高(35.34%),其余依次为长末端重复序列(LTR,5.43%)、短散布核元件(SINE,3.66%)、DNA转座元件(2.44%)和未知家族(0.56%)。LINE中的牛B型逆转录转座元件(RTE?BovB)占比达16.47%。转座元件在绵羊基因组中广泛分布,但在X染色体上富集显著,占比高达57.22%。研究通过Kimura双参数模型分析转座元件的演化历史,发现绵羊基因组经历了两次转座“爆发”事件:第1次以LINE转座元件为主,第2次则同时涉及LINE和SINE转座元件的扩增,且具有时序性。综上,LINE转座元件的持续活跃是绵羊基因组扩增的主要驱动力,且其演化历史与反刍动物的基因组进化密切相关。 展开更多
关键词 绵羊 基因组 长散布核元件(line) 基因组扩增 适应性进化
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Apoptosis of neoplasm cell lines induced byhepatic peptides extracted from sucking porcine hepatocytes 被引量:11
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作者 Kong XP Zou QY +3 位作者 Li RB Zheng PL Yang LP Jin SW 《World Journal of Gastroenterology》 SCIE CAS CSCD 1999年第5期435-439,共5页
关键词 NEOPLASM cell lines APOPTOSIS HEPATIC PEPTIDES HEPATIC extracts liver neoplasms HEPATOCYTES
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Effective Hybrid Teaching-learning-based Optimization Algorithm for Balancing Two-sided Assembly Lines with Multiple Constraints 被引量:8
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作者 TANG Qiuhua LI Zixiang +2 位作者 ZHANG Liping FLOUDAS C A CAO Xiaojun 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2015年第5期1067-1079,共13页
Due to the NP-hardness of the two-sided assembly line balancing (TALB) problem, multiple constraints existing in real applications are less studied, especially when one task is involved with several constraints. In ... Due to the NP-hardness of the two-sided assembly line balancing (TALB) problem, multiple constraints existing in real applications are less studied, especially when one task is involved with several constraints. In this paper, an effective hybrid algorithm is proposed to address the TALB problem with multiple constraints (TALB-MC). Considering the discrete attribute of TALB-MC and the continuous attribute of the standard teaching-learning-based optimization (TLBO) algorithm, the random-keys method is hired in task permutation representation, for the purpose of bridging the gap between them. Subsequently, a special mechanism for handling multiple constraints is developed. In the mechanism, the directions constraint of each task is ensured by the direction check and adjustment. The zoning constraints and the synchronism constraints are satisfied by teasing out the hidden correlations among constraints. The positional constraint is allowed to be violated to some extent in decoding and punished in cost fimction. Finally, with the TLBO seeking for the global optimum, the variable neighborhood search (VNS) is further hybridized to extend the local search space. The experimental results show that the proposed hybrid algorithm outperforms the late acceptance hill-climbing algorithm (LAHC) for TALB-MC in most cases, especially for large-size problems with multiple constraints, and demonstrates well balance between the exploration and the exploitation. This research proposes an effective and efficient algorithm for solving TALB-MC problem by hybridizing the TLBO and VNS. 展开更多
关键词 two-sided assembly line balancing teaching-learning-based optimization algorithm variable neighborhood search positional constraints zoning constraints synchronism constraints
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Genetic Relation Analysis on Ramie[Boehmeria nivea(L.)Gaud.]Inbred Lines by SRAP Markers 被引量:15
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作者 LIU Li-jun PENG Ding-xiang WANG Bo 《Agricultural Sciences in China》 CAS CSCD 2008年第8期944-949,共6页
The objective of this article is to reveal the variations of ramie inbred lines in DNA level and discuss their molecular background to provide a theoretical basis for ramie cross breeding. In the present study, the ge... The objective of this article is to reveal the variations of ramie inbred lines in DNA level and discuss their molecular background to provide a theoretical basis for ramie cross breeding. In the present study, the genetic relationships among 33 inbred line accessions and two wild types that originated from China and Brazil were estimated using sequence-related amplified polymorphism (SRAP) markers. The results showed that 33 out of 81 primer combinations turned out to be polymorphic and 332 polymorphism bands were obtained. On the basis of the appearance of the markers, the genetic relationships were analyzed using unweighted pair-group method of arithmetic average cluster analysis (UPGMA), and the genetic Jaccard similarity coefficients were calculated. The inbred-lines originating from China and Brazil formed a cluster suggesting a possibility that the Brazilian cultivars could have developed from cultivars introduced from China. Within ramie inbred-lines, the groupings also indicated that the greatest genetic relationship among cultivars was correlated to the region of origin of cultivars. The results provided the evidence that SRAP was an efficient approach, suitable for taxonomic analysis of ramie inbred lines, To the authors' knowledge, this is the first application of SRAP marker on the systematics of ramie inbred lines. 展开更多
关键词 relatives of ramie inbred lines SRAP taxonomic analysis UPGMA
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