Microhabitat heterogeneity results in significant variations in the thermal environment on a small spatial scale,leading to different intensities of cold stress during extreme low-temperature events.Investigating vari...Microhabitat heterogeneity results in significant variations in the thermal environment on a small spatial scale,leading to different intensities of cold stress during extreme low-temperature events.Investigating variations in body temperature and metabolomic responses of organisms inhabiting different microhabitats emerges as an important task for understanding how organisms respond to more frequent extreme low-temperature events in the face of climate change.In the present study,we measured substrate temperature,air temperature,wind speed,light intensity,and body temperature to evaluate the relative importance of drivers that affect body temperature in different microhabitats,and determined the metabolomic responses of intertidal snails Littorina brevicula and limpets Cellana toreuma from different microhabitats(snail:exposed vs.shaded rock;limpet,rock vs.tidal pool)during extreme low-temperature event in winter.Results showed that microhabitat type,substrate temperature,air temperature,wind speed,and light intensity contribute notably to the body temperatures.During extreme low-temperature events,mollusks collected from different microhabitats exhibited microhabitat-specific metabolomic responses that are associated with cellular stress response,energy metabolism,immune response,nucleotide metabolism,and osmoregulation.These metabolic pathways were highly induced in the more exposed areas(exposed rock for snails and rocky environment for limpets).Notably,in different microhabitats,the metabolites enriched from these pathways showed significant correlations with microclimate environmental variables(i.e.,substrate temperature,wind speed,and body tem-perature).Overall,these findings highlight the importance of microhabitat heterogeneity for intertidal species surviving extreme cold events and are essential for understanding cold adaptation of intertidal species in the context of climate change.展开更多
The physiological performance of a mid-intertidal limpet Cellana toreuma was determined to study the physiological adaptation of intertidal animals to rapid changes and extreme temperatures during emersion.The relatio...The physiological performance of a mid-intertidal limpet Cellana toreuma was determined to study the physiological adaptation of intertidal animals to rapid changes and extreme temperatures during emersion.The relationship between the Arrhenius breakpoint temperature(ABT)and in situ operative body temperature was studied to predict the possible impact of climate change on the species.The temperature coefficient(Q10)of emersed animals was higher than that of submersed animals and the ratio of aerial:aquatic heart rate rose with increasing temperature.The ABTs of submersed and emersed animals were 30.2 and 34.2°C,respectively.The heart rate and levels of molecular biomarkers(hsps,ampkα,ampkβand sirt1 mRNA)were determined in 48 h simulated semi-diurnal tides.There were no obvious changes of heart rate and gene expression during the transition between emersion and submersion at room temperature,although expressions of hsp70 and hsp90 were induced significantly after thermal stress.These results indicate that C.toreuma can effectively utilize atmospheric oxygen,and the higher Q10 and ABT of emersed animals are adaptations to the rapid change and extreme thermal stress during emersion.However,the in situ operative body temperature frequently exceeds the aerial ABT of C.toreuma,indicating the occurrence of large-scale mortality of C.toreuma in summer,and this species should be sensitive to increasing temperature in the scenario of climate change.展开更多
The predictability of high temperatures is important for intertidal species for coping with thermal stress.To investigate the roles of high temperature and its predictability on the population abundance and reproducti...The predictability of high temperatures is important for intertidal species for coping with thermal stress.To investigate the roles of high temperature and its predictability on the population abundance and reproductive output of an intertidal rocky shore limpet,Siphonaria japonica,we monitored the operative temperature,recorded the population abundance of egg ribbons and adults,and quantified the expression of heat shock protein 70(hsp70)mRNA on two warm-temperate rocky shores with different thermal environments.Abundances of limpets and egg ribbons in the hotter but more predictable(HP)habitats were higher than those in the relatively benign and unpredictable(BU)habitats.In the HP habitats,there was a strong positive correlation between habitat temperature and population abundance.For limpets living in the HP habitats,the expression of hsp70 exhibited a smaller increase with rising body temperature than for BU limpets,indicating the existence in HP limpets of a preparatory strategy in cellular stress response against thermal stress.Our results dem on strate that the predictability of operative temperature can affect physiological responses and population dynamics.The importance of predictability should be considered in analyses of the ecological consequences of climate warming.展开更多
基金supported by the National Natu-ral Science Foundation of China(42025604)the Fundamental Research Funds for the Central Universities of the Ocean University of China.
文摘Microhabitat heterogeneity results in significant variations in the thermal environment on a small spatial scale,leading to different intensities of cold stress during extreme low-temperature events.Investigating variations in body temperature and metabolomic responses of organisms inhabiting different microhabitats emerges as an important task for understanding how organisms respond to more frequent extreme low-temperature events in the face of climate change.In the present study,we measured substrate temperature,air temperature,wind speed,light intensity,and body temperature to evaluate the relative importance of drivers that affect body temperature in different microhabitats,and determined the metabolomic responses of intertidal snails Littorina brevicula and limpets Cellana toreuma from different microhabitats(snail:exposed vs.shaded rock;limpet,rock vs.tidal pool)during extreme low-temperature event in winter.Results showed that microhabitat type,substrate temperature,air temperature,wind speed,and light intensity contribute notably to the body temperatures.During extreme low-temperature events,mollusks collected from different microhabitats exhibited microhabitat-specific metabolomic responses that are associated with cellular stress response,energy metabolism,immune response,nucleotide metabolism,and osmoregulation.These metabolic pathways were highly induced in the more exposed areas(exposed rock for snails and rocky environment for limpets).Notably,in different microhabitats,the metabolites enriched from these pathways showed significant correlations with microclimate environmental variables(i.e.,substrate temperature,wind speed,and body tem-perature).Overall,these findings highlight the importance of microhabitat heterogeneity for intertidal species surviving extreme cold events and are essential for understanding cold adaptation of intertidal species in the context of climate change.
基金supported by grants from Nature Science funds for Distinguished Young Scholars of Fujian Province,China(2011J06017)National Natural Science Foundation of China(41076083,41276126)the Fundamental Research Funds for the Central Universities and the Program for New Century Excellent Talents in University of Fujian Province.We thank Dr Colin Little for constructive discussions and help with preparation of the manuscript.
文摘The physiological performance of a mid-intertidal limpet Cellana toreuma was determined to study the physiological adaptation of intertidal animals to rapid changes and extreme temperatures during emersion.The relationship between the Arrhenius breakpoint temperature(ABT)and in situ operative body temperature was studied to predict the possible impact of climate change on the species.The temperature coefficient(Q10)of emersed animals was higher than that of submersed animals and the ratio of aerial:aquatic heart rate rose with increasing temperature.The ABTs of submersed and emersed animals were 30.2 and 34.2°C,respectively.The heart rate and levels of molecular biomarkers(hsps,ampkα,ampkβand sirt1 mRNA)were determined in 48 h simulated semi-diurnal tides.There were no obvious changes of heart rate and gene expression during the transition between emersion and submersion at room temperature,although expressions of hsp70 and hsp90 were induced significantly after thermal stress.These results indicate that C.toreuma can effectively utilize atmospheric oxygen,and the higher Q10 and ABT of emersed animals are adaptations to the rapid change and extreme thermal stress during emersion.However,the in situ operative body temperature frequently exceeds the aerial ABT of C.toreuma,indicating the occurrence of large-scale mortality of C.toreuma in summer,and this species should be sensitive to increasing temperature in the scenario of climate change.
基金The study was support by National Natural Science Foundation of China(nos.41776135,41976142)Nature Science funds fbr Distinguished Young Scholars of Fujian Province,China(no.2017J07003).
文摘The predictability of high temperatures is important for intertidal species for coping with thermal stress.To investigate the roles of high temperature and its predictability on the population abundance and reproductive output of an intertidal rocky shore limpet,Siphonaria japonica,we monitored the operative temperature,recorded the population abundance of egg ribbons and adults,and quantified the expression of heat shock protein 70(hsp70)mRNA on two warm-temperate rocky shores with different thermal environments.Abundances of limpets and egg ribbons in the hotter but more predictable(HP)habitats were higher than those in the relatively benign and unpredictable(BU)habitats.In the HP habitats,there was a strong positive correlation between habitat temperature and population abundance.For limpets living in the HP habitats,the expression of hsp70 exhibited a smaller increase with rising body temperature than for BU limpets,indicating the existence in HP limpets of a preparatory strategy in cellular stress response against thermal stress.Our results dem on strate that the predictability of operative temperature can affect physiological responses and population dynamics.The importance of predictability should be considered in analyses of the ecological consequences of climate warming.
