In this paper, it was aimed to identified and quantified hesperidin and limonin compounds using HPLC （High Performance Liquid Chromatography） techniques against to developing of mal secco disease caused by Phoma tra...In this paper, it was aimed to identified and quantified hesperidin and limonin compounds using HPLC （High Performance Liquid Chromatography） techniques against to developing of mal secco disease caused by Phoma tracheiphila. Six citrus lemon varieties （Meyer, Kiitdiken, Enterdonato, Yediveren, Sweet lemon and Euroka） were infected by P. tracheiphila and artificial inoculation were applied in vivo conditions. Before and after inoculation, leaf, branch and stem samples were taken from each lemon varieties. The results show that the amount of hesperidin and limonin concentration was increased after the inoculations at various levels based on the lemon cultivars. Various concentrations （1, 5, 10, 25, 50, 75, 100 ppm） of hesperidin and limonin compounds were also tested under in vitro conditions to compare response of P. tracheiphila development. According to the results, hesperidin and limonin compounds play an important role against to P. tracheiphila development and Sweet Lemon variety was found to be the most resistance both observation and HPLC results.展开更多
Many economically important perennial species bear recalcitrant seeds, including tea, coffee, cocoa, mango,citrus, rubber, oil palm and coconut. Orthodox seeds can be dried almost completely without losing viability, ...Many economically important perennial species bear recalcitrant seeds, including tea, coffee, cocoa, mango,citrus, rubber, oil palm and coconut. Orthodox seeds can be dried almost completely without losing viability, but so-called recalcitrant seeds have a very limited storage life and die upon drying below a higher critical moisture content than orthodox seeds. As a result, the development of longterm storage methods for recalcitrant seeds is compromised.Lowering this critical moisture content would be very valuable since dry seed storage is the safest, most convenient and cheapest method for conserving plant genetic resources.Therefore, we have attempted to induce desiccation tolerance(DT) in the desiccation sensitive seeds of Citrus limon. We show that DT can be induced by paclobutrazol(an inhibitor of gibberellin biosynthesis) and we studied its associated transcriptome to delineate the molecular mechanisms underlying this induction of DT. Paclobutrazol not only interfered with gibberellin related gene expression but also caused extensive changes in expression of genes involved in the biosynthesis and signaling of other hormones. Paclobutrazol induced a transcriptomic switch encompassing suppression of biotic-and induction of abiotic responses.We hypothesize that this is the main driver of the induction of DT by paclobutrazol in C. limon seeds.展开更多
Plant long non-coding RNAs(lncRNAs)are emerging as important regulators of gene expression through various mechanisms viz.binding to double-stranded DNA(dsDNA),physically targeting mRNAs and also act as microRNA(miRNA...Plant long non-coding RNAs(lncRNAs)are emerging as important regulators of gene expression through various mechanisms viz.binding to double-stranded DNA(dsDNA),physically targeting mRNAs and also act as microRNA(miRNA)sponges.Characteristic secondary metabolites and their content determine the market value of Citrus plant products for culinary and industrial uses.Though lncRNAs are emerging as important molecular players that regulate secondary metabolites in plants but the roles of lncRNAs are yet unknown for the regulation of secondary metabolites in Citrus limon.In this study,a total of 11814 lncRNAs were identified from four RNA-seq datasets comprising of data from four different tissues of C.limon viz.leaf,bud,fruit and peel.Co-expression analysis of whole set of genes with identified lncRNAs revealed a total of 632 lncRNAs correlating with 5810 genes.Functional annotation of genes correlating with lncRNAs unveiled the association of 379 genes with the biosynthesis of secondary metabolite pathways and among them,36 and 12 genes are from terpenoids and flavonoids metabolism/biosynthesis pathways respectively.Expression correlation analysis unfolded the possible role of 113 lncRNAs in terpenoids metabolism and 29 lncRNAs in the flavonoids metabolic pathway.Seventy nine lncRNAs were predicted to take part in miRNA-mediated gene regulation,out of which 10 lncRNAs were predicted to act as endogenous target mimics(eTMs)for miRNAs and 74 lncRNAs were found to act as miRNA targets.Expression of six predicted lncRNAs and seven correlated genes involved in terpenoids and flavonoids metabolism were validated using semi-quantitative RT-PCR.展开更多
Seasonal incidence of citrus psylla, Diaphorina citri Kuwayama was studied in Assam Lemon (Citrus limon L. Burm) in two consecutive years in sub-Himalayan plains (terai zone) of West Bengal (India). The psyllids appea...Seasonal incidence of citrus psylla, Diaphorina citri Kuwayama was studied in Assam Lemon (Citrus limon L. Burm) in two consecutive years in sub-Himalayan plains (terai zone) of West Bengal (India). The psyllids appeared with the arrival of new flush which occurred three times a year, the first during January last to early February (4th-5th SW), second during April-May (17th - 18th SW) and the third during September (37th-38th SW). Three peaks were recorded—one at 11th SW (6.11 psyllids/shoot), the second at 21st SW (7.50 psyllids/shoot) and the third peak at 43rd SW (5.06 psyllids/shoot). The second peak recorded the highest psyllid population. The psylla were active throughout the whole year except during the cold winter period when their population was either low or absent. Temperature showed a positive association with psyllid population (r = 0.304 for TMax and r = 0.182 for TMin) while RH% (r = −0.200 for RHMor and r = −0.200 for RHEvn) and rainfall had a negative correlation (r = −0.013). During the 1st peak population (11th SW) the average temperature, RH% and rainfall were 30.82˚C (TMax) and 16.68˚C (TMin), 55.36% (RHMor) and 62.72% (RHEvn) and nought respectively;while the corresponding values were 33.43˚C (TMax) and 24.29˚C (TMin), 76.43% (RHMor) and 75% (RHEvn) and 7.85 mm respectively during the 2nd peak (21st SW). Likewise, at the 3rd peak (43rd SW), the respective values were 30.22˚C and 19.07˚C (TMax and TMin, respectively), 80% and 76.79% (RHMor and RHEvn, respectively) and zero mm respectively.展开更多
文摘In this paper, it was aimed to identified and quantified hesperidin and limonin compounds using HPLC （High Performance Liquid Chromatography） techniques against to developing of mal secco disease caused by Phoma tracheiphila. Six citrus lemon varieties （Meyer, Kiitdiken, Enterdonato, Yediveren, Sweet lemon and Euroka） were infected by P. tracheiphila and artificial inoculation were applied in vivo conditions. Before and after inoculation, leaf, branch and stem samples were taken from each lemon varieties. The results show that the amount of hesperidin and limonin concentration was increased after the inoculations at various levels based on the lemon cultivars. Various concentrations （1, 5, 10, 25, 50, 75, 100 ppm） of hesperidin and limonin compounds were also tested under in vitro conditions to compare response of P. tracheiphila development. According to the results, hesperidin and limonin compounds play an important role against to P. tracheiphila development and Sweet Lemon variety was found to be the most resistance both observation and HPLC results.
基金financial support from CNPq-National Council for Scientific and Technological Development Brazil(246220/2012-0)
文摘Many economically important perennial species bear recalcitrant seeds, including tea, coffee, cocoa, mango,citrus, rubber, oil palm and coconut. Orthodox seeds can be dried almost completely without losing viability, but so-called recalcitrant seeds have a very limited storage life and die upon drying below a higher critical moisture content than orthodox seeds. As a result, the development of longterm storage methods for recalcitrant seeds is compromised.Lowering this critical moisture content would be very valuable since dry seed storage is the safest, most convenient and cheapest method for conserving plant genetic resources.Therefore, we have attempted to induce desiccation tolerance(DT) in the desiccation sensitive seeds of Citrus limon. We show that DT can be induced by paclobutrazol(an inhibitor of gibberellin biosynthesis) and we studied its associated transcriptome to delineate the molecular mechanisms underlying this induction of DT. Paclobutrazol not only interfered with gibberellin related gene expression but also caused extensive changes in expression of genes involved in the biosynthesis and signaling of other hormones. Paclobutrazol induced a transcriptomic switch encompassing suppression of biotic-and induction of abiotic responses.We hypothesize that this is the main driver of the induction of DT by paclobutrazol in C. limon seeds.
基金KSB thankfully acknowledge fellowship support received from Council of Scientific and Industrial Research,India(Award no.09/059(0064)/2018-EMR-I).The authors are thankful to DST,Govt.of India for providing DST-FIST Support to Department of Botany,Gauhati University,where this research work was carried out.
文摘Plant long non-coding RNAs(lncRNAs)are emerging as important regulators of gene expression through various mechanisms viz.binding to double-stranded DNA(dsDNA),physically targeting mRNAs and also act as microRNA(miRNA)sponges.Characteristic secondary metabolites and their content determine the market value of Citrus plant products for culinary and industrial uses.Though lncRNAs are emerging as important molecular players that regulate secondary metabolites in plants but the roles of lncRNAs are yet unknown for the regulation of secondary metabolites in Citrus limon.In this study,a total of 11814 lncRNAs were identified from four RNA-seq datasets comprising of data from four different tissues of C.limon viz.leaf,bud,fruit and peel.Co-expression analysis of whole set of genes with identified lncRNAs revealed a total of 632 lncRNAs correlating with 5810 genes.Functional annotation of genes correlating with lncRNAs unveiled the association of 379 genes with the biosynthesis of secondary metabolite pathways and among them,36 and 12 genes are from terpenoids and flavonoids metabolism/biosynthesis pathways respectively.Expression correlation analysis unfolded the possible role of 113 lncRNAs in terpenoids metabolism and 29 lncRNAs in the flavonoids metabolic pathway.Seventy nine lncRNAs were predicted to take part in miRNA-mediated gene regulation,out of which 10 lncRNAs were predicted to act as endogenous target mimics(eTMs)for miRNAs and 74 lncRNAs were found to act as miRNA targets.Expression of six predicted lncRNAs and seven correlated genes involved in terpenoids and flavonoids metabolism were validated using semi-quantitative RT-PCR.
文摘Seasonal incidence of citrus psylla, Diaphorina citri Kuwayama was studied in Assam Lemon (Citrus limon L. Burm) in two consecutive years in sub-Himalayan plains (terai zone) of West Bengal (India). The psyllids appeared with the arrival of new flush which occurred three times a year, the first during January last to early February (4th-5th SW), second during April-May (17th - 18th SW) and the third during September (37th-38th SW). Three peaks were recorded—one at 11th SW (6.11 psyllids/shoot), the second at 21st SW (7.50 psyllids/shoot) and the third peak at 43rd SW (5.06 psyllids/shoot). The second peak recorded the highest psyllid population. The psylla were active throughout the whole year except during the cold winter period when their population was either low or absent. Temperature showed a positive association with psyllid population (r = 0.304 for TMax and r = 0.182 for TMin) while RH% (r = −0.200 for RHMor and r = −0.200 for RHEvn) and rainfall had a negative correlation (r = −0.013). During the 1st peak population (11th SW) the average temperature, RH% and rainfall were 30.82˚C (TMax) and 16.68˚C (TMin), 55.36% (RHMor) and 62.72% (RHEvn) and nought respectively;while the corresponding values were 33.43˚C (TMax) and 24.29˚C (TMin), 76.43% (RHMor) and 75% (RHEvn) and 7.85 mm respectively during the 2nd peak (21st SW). Likewise, at the 3rd peak (43rd SW), the respective values were 30.22˚C and 19.07˚C (TMax and TMin, respectively), 80% and 76.79% (RHMor and RHEvn, respectively) and zero mm respectively.
