Rheumatoid arthritis(RA)is a refractory autoimmune disease with limited treatment options.Plantderived exosomes-like nanovesicles(PDENs)have emerged as a novel nanomedical approach,with the inherent bioactive compound...Rheumatoid arthritis(RA)is a refractory autoimmune disease with limited treatment options.Plantderived exosomes-like nanovesicles(PDENs)have emerged as a novel nanomedical approach,with the inherent bioactive compounds from their source plants.The roots of Morinda officinalis How.(MO),a Chinese herb,exhibit notable anti-inflammatory activities and hold promising therapeutic value.We engineered a joint-targe ting delivery system(termed MOE@EM)by masking MO-derived exosomes-like nanovesicles(MOE)with erythrocyte membrane(EM).This biomimetic strategy,using EM camouflage,is intended to improve the in vivo fate of MOE.We investigated the antioxidative and anti-inflammatory activities,immunogenicity,drug accumulation in the joint,and therapeutic efficacy to ascertain its suitability for RA therapy.UV irradiation significantly increased the activities of catalase and peroxidase of MOE,and enhanced the anti-inflammatory effects via the Wnt/β-catenin pathway.Furthermore,MOE@EM markedly attenuated dendritic cell activation.MOE@EM exhibited joint-specific delivery,with substantial reduction in paw swelling,and favorable modulation of immune microenvironment.展开更多
基金supported by the National Key Research and Development Program of China(Nos.2021YFC2400600,2022YFE0203600)National Natural Science Foundation of China(Nos.81925035,82304842,82204628)+6 种基金High-level Innovative Research Institute(No.2021B0909050003)Chinese Academy of Sciences President’s International Fellowship Initiative(No.2024VBB0004)the Scientific and Technological Innovation Projects in Zhongshan City(Nos.LJ2021001,CXTD2022011)the Social Welfare and Basic Research Projects in Zhongshan(No.221014134359625)the Special Projects in Key Areas of Colleges and Universities in Guangdong Province(No.2022ZDZX2015)the Science and Technology Program of Guangzhou(No.2024A04J4899)Young Talent Project of Guangzhou University of Chinese Medicine(No.A1–2601–24–414–110Z76)。
文摘Rheumatoid arthritis(RA)is a refractory autoimmune disease with limited treatment options.Plantderived exosomes-like nanovesicles(PDENs)have emerged as a novel nanomedical approach,with the inherent bioactive compounds from their source plants.The roots of Morinda officinalis How.(MO),a Chinese herb,exhibit notable anti-inflammatory activities and hold promising therapeutic value.We engineered a joint-targe ting delivery system(termed MOE@EM)by masking MO-derived exosomes-like nanovesicles(MOE)with erythrocyte membrane(EM).This biomimetic strategy,using EM camouflage,is intended to improve the in vivo fate of MOE.We investigated the antioxidative and anti-inflammatory activities,immunogenicity,drug accumulation in the joint,and therapeutic efficacy to ascertain its suitability for RA therapy.UV irradiation significantly increased the activities of catalase and peroxidase of MOE,and enhanced the anti-inflammatory effects via the Wnt/β-catenin pathway.Furthermore,MOE@EM markedly attenuated dendritic cell activation.MOE@EM exhibited joint-specific delivery,with substantial reduction in paw swelling,and favorable modulation of immune microenvironment.
