Since the Industrial Revolution,the pursuit of lighting for humanity has gradually evolved into an out-of-control disaster of light pollution.Artificial light at night(ALAN)scatters the constituents of the atmosphere(...Since the Industrial Revolution,the pursuit of lighting for humanity has gradually evolved into an out-of-control disaster of light pollution.Artificial light at night(ALAN)scatters the constituents of the atmosphere(such as gas molecules,aerosols,and clouds),causing the artificial glow to brighten the night sky during observations.1 The unrestrained expansion of urban lighting systems and the unrestrained flashing of commercial neon lights have led to an annual increase of 6%in the brightness of the night sky.Around the city,people can no longer see the Milky Way with the naked eye.This kind of light pollution not only obscures the starry sky but also disrupts the circadian rhythm that has formed in the ecosystem of Earth over millions of years;every year,millions of birds lose their way due to the strong light in cities and crash into tall buildings.The foraging and breeding behaviors of nocturnal animals have been severely disrupted,and biodiversity continues to decline.Human health itself is also threatened.Excessive night light can inhibit the secretion of melatonin and increase the risk of cancer and cardiovascular diseases.展开更多
Background Primary systemic light chain amyloidosis （AL） is a rare plasma cell disease,our purpose was to analyze the immunophenotypic characteristics of the plasma cells in bone marrow in AL patients,and explore wh...Background Primary systemic light chain amyloidosis （AL） is a rare plasma cell disease,our purpose was to analyze the immunophenotypic characteristics of the plasma cells in bone marrow in AL patients,and explore whether the detection of abnormal plasma cell clones in bone marrow by flow cytometry （FCM） could be used as an important indicator of AL diagnosis.Methods Fresh bone marrow samples were collected from 51 AL,21 multiple myeloma （MM）,and 5 Waldenstr（o）m＇s macroglobulinemia （WM） patients.The immunophenotype of bone marrow cells were analyzed and compared by FCM using a panel of antibodies including CD45,CD38,CD138,CD117,CD56,and CD19.Results In AL,light chain restriction could be identified in 31 cases （60.9%）,in which the λ light chain restriction was found in 24 cases （77.4%）.In MM,κ light chain restriction was found in 13 cases （61.9%）,and λ light chain restriction in eight cases.CD45 on abnormal plasma cells was negative to weakly positive in both AL and MM,but was positive to strongly positive in WM.In the bone marrow plasma cells of the 51 AL,78.4% were CD56＋,68.6% were CD117＋,and 88.2% were CD19-.While in the 21 MM cases,66.7% were CD56＋,38.1% were CD117＋,and 90.4% were CD19-.The plasmacytoid lymphocytes in the five WM patients were CD19＋ and CD56-,CD117-.Conclusion Detection of abnormal plasma cell clones in bone marrow by FCM is valuable for the diagnosis of AL.展开更多
基金supported by Strategic Priority Research Program of the Chinese Academy of Sciences grant XDB0550100by the Foundation of China Association for Science and Technology(CAST)under grants kpbwh-2023-3-03 and kpbwh-2024-2-08.
文摘Since the Industrial Revolution,the pursuit of lighting for humanity has gradually evolved into an out-of-control disaster of light pollution.Artificial light at night(ALAN)scatters the constituents of the atmosphere(such as gas molecules,aerosols,and clouds),causing the artificial glow to brighten the night sky during observations.1 The unrestrained expansion of urban lighting systems and the unrestrained flashing of commercial neon lights have led to an annual increase of 6%in the brightness of the night sky.Around the city,people can no longer see the Milky Way with the naked eye.This kind of light pollution not only obscures the starry sky but also disrupts the circadian rhythm that has formed in the ecosystem of Earth over millions of years;every year,millions of birds lose their way due to the strong light in cities and crash into tall buildings.The foraging and breeding behaviors of nocturnal animals have been severely disrupted,and biodiversity continues to decline.Human health itself is also threatened.Excessive night light can inhibit the secretion of melatonin and increase the risk of cancer and cardiovascular diseases.
基金This work was funded by the National Natural Science Foundation of China （No.81370612） and the Youth Fund Project of the National Natural Science Foundation of China （No.81102211）.
文摘Background Primary systemic light chain amyloidosis （AL） is a rare plasma cell disease,our purpose was to analyze the immunophenotypic characteristics of the plasma cells in bone marrow in AL patients,and explore whether the detection of abnormal plasma cell clones in bone marrow by flow cytometry （FCM） could be used as an important indicator of AL diagnosis.Methods Fresh bone marrow samples were collected from 51 AL,21 multiple myeloma （MM）,and 5 Waldenstr（o）m＇s macroglobulinemia （WM） patients.The immunophenotype of bone marrow cells were analyzed and compared by FCM using a panel of antibodies including CD45,CD38,CD138,CD117,CD56,and CD19.Results In AL,light chain restriction could be identified in 31 cases （60.9%）,in which the λ light chain restriction was found in 24 cases （77.4%）.In MM,κ light chain restriction was found in 13 cases （61.9%）,and λ light chain restriction in eight cases.CD45 on abnormal plasma cells was negative to weakly positive in both AL and MM,but was positive to strongly positive in WM.In the bone marrow plasma cells of the 51 AL,78.4% were CD56＋,68.6% were CD117＋,and 88.2% were CD19-.While in the 21 MM cases,66.7% were CD56＋,38.1% were CD117＋,and 90.4% were CD19-.The plasmacytoid lymphocytes in the five WM patients were CD19＋ and CD56-,CD117-.Conclusion Detection of abnormal plasma cell clones in bone marrow by FCM is valuable for the diagnosis of AL.
